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The map label for this gene is pcaB [H]

Identifier: 17546970

GI number: 17546970

Start: 2443255

End: 2444616

Strand: Reverse

Name: pcaB [H]

Synonym: RSc2251

Alternate gene names: 17546970

Gene position: 2444616-2443255 (Counterclockwise)

Preceding gene: 17546971

Following gene: 17546969

Centisome position: 65.78

GC content: 74.96

Gene sequence:

>1362_bases
ATGATTACCGCACTGCTCGATCGTACCTTCTCCACACCGGCCATGCTGGCGGTGTGGTCCGACGCGGCCACCGTGCGCGC
GATGCTCGACGTGGAGGCCGCGCTGGCGCAGGCCGAGGGCGAGGTCGGCGTGATCCCGGCGCAAGCCGTGGCGCCCATCC
GCGCGGTGTGCGCCAGGGCGACGCTGGACCTGGAGGCGCTCGGCGCGGCCGCGGCCGGCGCGGGCAACCTCGCGATTCCG
CTGGTCAAACAGCTGACCGCCGCCGTCGCGCAGCACGACGAACTCGCCGCGCGCTATGTGCACTGGGGCGCGACCAGTCA
GGACATCATCGATACCGGCCTGATGCTGCAATGGCGGCAGGCGGCCGCGCTGCTCGACGCCGATCTGCGGCAACTGGCCG
ACGCGCTGGCCGCGCTGGCTCGCGTGCATCGCGATACGCCGATGGTCGCGCGCACCTGGCTGCAGCAGGCGCTGCCGACC
ACCTTCGGCCGCAAGGTGGCCGGCTGGCTGGAGGCGGTGCATCGCGCGCAGGACCGCCTGGCCGCGCTGCGCGCGCATGT
GCCCGCGCTGCAGTTCGGCGGCGCGGCCGGCACGCTGGCGAGCCTCGGCACGCAAGGCCGGGCCGTGGCGCAGGCTTTGG
CGCAGGCGCTCGATCTGCCGCTGCCCGCGGCGTCGTGGCATGGCGAGCAGGACCGCATCGCCGACATCGGCGCCGCGCTG
GCTTTGCTGACCGGCACGCTTGGCCACATGGCGCGCGACCTGTCGCTGATGATGCAGACCGAGGTGGGCGAGGTGGCCGA
GCCGGCGGGTGCAGGCAAGGGCGGCTCATCCACCATGCCGCACAAGCGCAACCCGGTCGGCTGTGCCACCGTGCTGGCGG
CGGCCACGCGCATGCCGGGGCTGGCGGCGACCCTGCTGGCCGCGCTGCCGCAGGAGCACGAGCGCGCGCTCGGCGGCTGG
CAGGCGCAATGGGCCACGCTGCGCGAGATGGCGTGCCTCGCCGCCGGCGCGCTGGACCGCATGCGCGAGCTCATCGCCGG
GCTGAACGTCGACGCCGCGCGGATGCGCGCCAACCTCGACCTGACGCGCGGCCTGATCCTGGCCGAAGCGGTGATGCTCG
CCCTGGGCGCCGACCTGGGCCGCCTGCAGGCGCACCACGTGGTGGAGGCCGCCAGCCGCCTGGCTGTCCGGCAGCACCGG
ACGCTGCGCGACGTGCTGGCCGAAGACGCCGACGTGCTGCGCGCCTGGGGCGATGCGGCGCCGCAGCGGCTGGACGCGCT
GCTCGATCCCGCCCACTACCTCGGCGACGCGGCGGCCGCCGTCGACCGCGTGCTGGCGGAACACGACCGTCGCCGCGCCT
GA

Upstream 100 bases:

>100_bases
CTGCAGCGCACCGGCGGCCGCTATGCGCTGTGCACGATGTGCATCGGCGTCGGCCAGGGCATCGCGCTGGTGATCGAACG
CGTGTGAACACGGACGCGCC

Downstream 100 bases:

>100_bases
ACCCGACTCCCACGGAGACACCATGCCCTGGCTTGAACACGACAACGTCCGCCTCTACCACGAATTCGACGATGGCCCTG
CCCCGGCCAAGCCGGTGCTG

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MITALLDRTFSTPAMLAVWSDAATVRAMLDVEAALAQAEGEVGVIPAQAVAPIRAVCARATLDLEALGAAAAGAGNLAIP
LVKQLTAAVAQHDELAARYVHWGATSQDIIDTGLMLQWRQAAALLDADLRQLADALAALARVHRDTPMVARTWLQQALPT
TFGRKVAGWLEAVHRAQDRLAALRAHVPALQFGGAAGTLASLGTQGRAVAQALAQALDLPLPAASWHGEQDRIADIGAAL
ALLTGTLGHMARDLSLMMQTEVGEVAEPAGAGKGGSSTMPHKRNPVGCATVLAAATRMPGLAATLLAALPQEHERALGGW
QAQWATLREMACLAAGALDRMRELIAGLNVDAARMRANLDLTRGLILAEAVMLALGADLGRLQAHHVVEAASRLAVRQHR
TLRDVLAEDADVLRAWGDAAPQRLDALLDPAHYLGDAAAAVDRVLAEHDRRRA

Sequences:

>Translated_453_residues
MITALLDRTFSTPAMLAVWSDAATVRAMLDVEAALAQAEGEVGVIPAQAVAPIRAVCARATLDLEALGAAAAGAGNLAIP
LVKQLTAAVAQHDELAARYVHWGATSQDIIDTGLMLQWRQAAALLDADLRQLADALAALARVHRDTPMVARTWLQQALPT
TFGRKVAGWLEAVHRAQDRLAALRAHVPALQFGGAAGTLASLGTQGRAVAQALAQALDLPLPAASWHGEQDRIADIGAAL
ALLTGTLGHMARDLSLMMQTEVGEVAEPAGAGKGGSSTMPHKRNPVGCATVLAAATRMPGLAATLLAALPQEHERALGGW
QAQWATLREMACLAAGALDRMRELIAGLNVDAARMRANLDLTRGLILAEAVMLALGADLGRLQAHHVVEAASRLAVRQHR
TLRDVLAEDADVLRAWGDAAPQRLDALLDPAHYLGDAAAAVDRVLAEHDRRRA
>Mature_453_residues
MITALLDRTFSTPAMLAVWSDAATVRAMLDVEAALAQAEGEVGVIPAQAVAPIRAVCARATLDLEALGAAAAGAGNLAIP
LVKQLTAAVAQHDELAARYVHWGATSQDIIDTGLMLQWRQAAALLDADLRQLADALAALARVHRDTPMVARTWLQQALPT
TFGRKVAGWLEAVHRAQDRLAALRAHVPALQFGGAAGTLASLGTQGRAVAQALAQALDLPLPAASWHGEQDRIADIGAAL
ALLTGTLGHMARDLSLMMQTEVGEVAEPAGAGKGGSSTMPHKRNPVGCATVLAAATRMPGLAATLLAALPQEHERALGGW
QAQWATLREMACLAAGALDRMRELIAGLNVDAARMRANLDLTRGLILAEAVMLALGADLGRLQAHHVVEAASRLAVRQHR
TLRDVLAEDADVLRAWGDAAPQRLDALLDPAHYLGDAAAAVDRVLAEHDRRRA

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Escherichia coli, GI1790398, Length=322, Percent_Identity=27.639751552795, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=377, Percent_Identity=24.1379310344828, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323391, Length=375, Percent_Identity=24.5333333333333, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24647570, Length=381, Percent_Identity=27.0341207349081, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 47527; Mature: 47527

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITALLDRTFSTPAMLAVWSDAATVRAMLDVEAALAQAEGEVGVIPAQAVAPIRAVCARA
CCHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHH
TLDLEALGAAAAGAGNLAIPLVKQLTAAVAQHDELAARYVHWGATSQDIIDTGLMLQWRQ
HCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AAALLDADLRQLADALAALARVHRDTPMVARTWLQQALPTTFGRKVAGWLEAVHRAQDRL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALRAHVPALQFGGAAGTLASLGTQGRAVAQALAQALDLPLPAASWHGEQDRIADIGAAL
HHHHHHCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ALLTGTLGHMARDLSLMMQTEVGEVAEPAGAGKGGSSTMPHKRNPVGCATVLAAATRMPG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
LAATLLAALPQEHERALGGWQAQWATLREMACLAAGALDRMRELIAGLNVDAARMRANLD
HHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCH
LTRGLILAEAVMLALGADLGRLQAHHVVEAASRLAVRQHRTLRDVLAEDADVLRAWGDAA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQRLDALLDPAHYLGDAAAAVDRVLAEHDRRRA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITALLDRTFSTPAMLAVWSDAATVRAMLDVEAALAQAEGEVGVIPAQAVAPIRAVCARA
CCHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHH
TLDLEALGAAAAGAGNLAIPLVKQLTAAVAQHDELAARYVHWGATSQDIIDTGLMLQWRQ
HCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AAALLDADLRQLADALAALARVHRDTPMVARTWLQQALPTTFGRKVAGWLEAVHRAQDRL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALRAHVPALQFGGAAGTLASLGTQGRAVAQALAQALDLPLPAASWHGEQDRIADIGAAL
HHHHHHCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ALLTGTLGHMARDLSLMMQTEVGEVAEPAGAGKGGSSTMPHKRNPVGCATVLAAATRMPG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
LAATLLAALPQEHERALGGWQAQWATLREMACLAAGALDRMRELIAGLNVDAARMRANLD
HHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCH
LTRGLILAEAVMLALGADLGRLQAHHVVEAASRLAVRQHRTLRDVLAEDADVLRAWGDAA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQRLDALLDPAHYLGDAAAAVDRVLAEHDRRRA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9582432; 12597275 [H]