BacMap

Definition	Ralstonia solanacearum GMI1000, complete genome.
Accession	NC_003295
Length	3,716,413

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The map label for this gene is ligD [H]

Identifier: 17546488

GI number: 17546488

Start: 1896181

End: 1897083

Strand: Direct

Name: ligD [H]

Synonym: RSc1769

Alternate gene names: 17546488

Gene position: 1896181-1897083 (Clockwise)

Preceding gene: 17546487

Following gene: 17546489

Centisome position: 51.02

GC content: 67.88

Gene sequence:

>903_bases
ATGAAGCAATTCGCGGGCGGCGTGGCCGTCATCACGGGCGGTGCATCGGGCTTCGGCAAGGCATTCGCCAGGCTCGGCGC
CGGACTGGGCATGAAGCTGGTGCTGGCCGACATCCAGCAGGACGCGCTGGATGCCGCCGTGGCCGGGTTCCGGGCGCAGG
GCGTCGAGGTCATCGGCGTGCGCGTCGACGTGTCCAGGGAGGCGGACGTGCAGGCGCTGGCCGATGCGGCCATCCGGACC
TTCGGCAAGGTCAACCTGCTGTTCAACAACGCCGGGGTCGGGGCGGGCGGCCTGATCTGGGAGAACTCCCAACAGGACTG
GGACTGGGTGCTCGGCGTCAACCTGCATGGCGTGATCCACGGCGTGCGCATCTTCACGCCGCTGATGCTGGCCGAGGCCG
CGAAGGACCCGTCCTACGCGGGCCATATCGTCAACACGGCGTCGATGGCGGGGCTGCTCAATGCGCCGGCCATGGGCATC
TACAACGTCTCCAAGCACGCCGTGGTGGCGCTGACGGAGTCGCTCTACCAGGACCTGAGCCTGGTGACGGAGCAGATCCG
CTGCTCGGTGCTGTGTCCGTATTTCGTGCCGACCGGCATCACGCAGTCGCATCGCAACCGCCCGCAGGACCTGGCCAATG
CCGCGCCGCCCACCCGGTCGCAGCTGGTGGCCCAGGCCATGACCGACAAGGCCGTCAGCGCCGGCAAGGTGAGCGCCGAG
CAGGTCGGCCAGATGACGTTCGATGCCATCCGGGACGAACGTTTCTATATTTATTCGCATCCGCACGCGCTGGCGCCGGT
GCAGCACCGCTTTGAAGACATCGTCTCGCAGCGCAACCCGTCCGATCCGTTCGAGGGCAAGCCGGACGTGCGGGCGCGTC
TGGTCGAGTCGCTGCGCGGCTGA

Upstream 100 bases:

>100_bases
TCGGCTATGCGGTGCGCAAGGTCGAGCCGGCCGGCTGACCCGGCCGCGCCGATCGCTTTTTACAATCCGGGTCCATGACG
ACAACACGAGGAAGACACGT

Downstream 100 bases:

>100_bases
CCGGACACGCCGCCTGCCGACGCCCCCCGAAACCTGGAGATCGATATGACCGCTGCCACCGCCACCCCCGTTGCCGCAAT
CACGCATCCGCAGTTCGCCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKQFAGGVAVITGGASGFGKAFARLGAGLGMKLVLADIQQDALDAAVAGFRAQGVEVIGVRVDVSREADVQALADAAIRT
FGKVNLLFNNAGVGAGGLIWENSQQDWDWVLGVNLHGVIHGVRIFTPLMLAEAAKDPSYAGHIVNTASMAGLLNAPAMGI
YNVSKHAVVALTESLYQDLSLVTEQIRCSVLCPYFVPTGITQSHRNRPQDLANAAPPTRSQLVAQAMTDKAVSAGKVSAE
QVGQMTFDAIRDERFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVESLRG

Sequences:

>Translated_300_residues
MKQFAGGVAVITGGASGFGKAFARLGAGLGMKLVLADIQQDALDAAVAGFRAQGVEVIGVRVDVSREADVQALADAAIRT
FGKVNLLFNNAGVGAGGLIWENSQQDWDWVLGVNLHGVIHGVRIFTPLMLAEAAKDPSYAGHIVNTASMAGLLNAPAMGI
YNVSKHAVVALTESLYQDLSLVTEQIRCSVLCPYFVPTGITQSHRNRPQDLANAAPPTRSQLVAQAMTDKAVSAGKVSAE
QVGQMTFDAIRDERFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVESLRG
>Mature_300_residues
MKQFAGGVAVITGGASGFGKAFARLGAGLGMKLVLADIQQDALDAAVAGFRAQGVEVIGVRVDVSREADVQALADAAIRT
FGKVNLLFNNAGVGAGGLIWENSQQDWDWVLGVNLHGVIHGVRIFTPLMLAEAAKDPSYAGHIVNTASMAGLLNAPAMGI
YNVSKHAVVALTESLYQDLSLVTEQIRCSVLCPYFVPTGITQSHRNRPQDLANAAPPTRSQLVAQAMTDKAVSAGKVSAE
QVGQMTFDAIRDERFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVESLRG

Specific function: Catalyzes the C alpha dehydrogenation of arylglycerol- beta-aryl ether (C alpha alcohol type) (compound IV) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI31542939, Length=200, Percent_Identity=30, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI40807363, Length=204, Percent_Identity=29.9019607843137, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI142976729, Length=203, Percent_Identity=30.5418719211823, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI25282469, Length=253, Percent_Identity=28.8537549407115, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI5031737, Length=200, Percent_Identity=31, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI32483357, Length=200, Percent_Identity=28.5, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI33667109, Length=203, Percent_Identity=31.0344827586207, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI109715829, Length=205, Percent_Identity=28.780487804878, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI66933014, Length=249, Percent_Identity=26.1044176706827, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310128830, Length=215, Percent_Identity=25.1162790697674, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310115468, Length=215, Percent_Identity=25.1162790697674, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI210032110, Length=232, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI224922801, Length=171, Percent_Identity=29.8245614035088, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI40254992, Length=195, Percent_Identity=29.7435897435897, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4758504, Length=218, Percent_Identity=29.3577981651376, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI83715985, Length=185, Percent_Identity=30.8108108108108, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI20149619, Length=200, Percent_Identity=27.5, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87082100, Length=213, Percent_Identity=30.9859154929577, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082160, Length=206, Percent_Identity=29.126213592233, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=29.7619047619048, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787335, Length=201, Percent_Identity=24.3781094527363, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790717, Length=200, Percent_Identity=25, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789208, Length=225, Percent_Identity=25.7777777777778, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789057, Length=175, Percent_Identity=29.7142857142857, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=234, Percent_Identity=29.9145299145299, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI193203115, Length=235, Percent_Identity=30.2127659574468, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508895, Length=193, Percent_Identity=30.0518134715026, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564282, Length=200, Percent_Identity=27.5, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=203, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=212, Percent_Identity=28.3018867924528, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=209, Percent_Identity=32.0574162679426, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=206, Percent_Identity=27.6699029126214, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=216, Percent_Identity=28.2407407407407, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=215, Percent_Identity=27.4418604651163, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=246, Percent_Identity=28.0487804878049, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=196, Percent_Identity=26.0204081632653, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=207, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=213, Percent_Identity=26.2910798122066, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=223, Percent_Identity=29.1479820627803, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=222, Percent_Identity=29.2792792792793, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=213, Percent_Identity=27.2300469483568, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323882, Length=203, Percent_Identity=28.0788177339901, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355319, Length=204, Percent_Identity=32.3529411764706, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644339, Length=191, Percent_Identity=29.8429319371728, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17737361, Length=192, Percent_Identity=32.2916666666667, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24581726, Length=209, Percent_Identity=30.1435406698565, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281364892, Length=229, Percent_Identity=27.9475982532751, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364494, Length=256, Percent_Identity=28.90625, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357041, Length=199, Percent_Identity=29.1457286432161, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI18859837, Length=205, Percent_Identity=30.2439024390244, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28571526, Length=201, Percent_Identity=28.8557213930348, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI23397609, Length=200, Percent_Identity=32.5, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21355631, Length=199, Percent_Identity=27.6381909547739, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571387, Length=207, Percent_Identity=29.4685990338164, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640492, Length=247, Percent_Identity=23.4817813765182, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24639444, Length=197, Percent_Identity=28.4263959390863, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221510726, Length=196, Percent_Identity=29.0816326530612, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007236, Length=230, Percent_Identity=27.3913043478261, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24641388, Length=210, Percent_Identity=32.3809523809524, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 32016; Mature: 32016

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFAGGVAVITGGASGFGKAFARLGAGLGMKLVLADIQQDALDAAVAGFRAQGVEVIGV
CCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
RVDVSREADVQALADAAIRTFGKVNLLFNNAGVGAGGLIWENSQQDWDWVLGVNLHGVIH
EEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCEEEEECCHHHHHH
GVRIFTPLMLAEAAKDPSYAGHIVNTASMAGLLNAPAMGIYNVSKHAVVALTESLYQDLS
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHH
LVTEQIRCSVLCPYFVPTGITQSHRNRPQDLANAAPPTRSQLVAQAMTDKAVSAGKVSAE
HHHHHHHHHEECCHHCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHH
QVGQMTFDAIRDERFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVESLRG
HHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKQFAGGVAVITGGASGFGKAFARLGAGLGMKLVLADIQQDALDAAVAGFRAQGVEVIGV
CCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
RVDVSREADVQALADAAIRTFGKVNLLFNNAGVGAGGLIWENSQQDWDWVLGVNLHGVIH
EEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCEEEEECCHHHHHH
GVRIFTPLMLAEAAKDPSYAGHIVNTASMAGLLNAPAMGIYNVSKHAVVALTESLYQDLS
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHH
LVTEQIRCSVLCPYFVPTGITQSHRNRPQDLANAAPPTRSQLVAQAMTDKAVSAGKVSAE
HHHHHHHHHEECCHHCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHH
QVGQMTFDAIRDERFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVESLRG
HHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726; 7764263 [H]