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The map label for this gene is thiG [H]

Identifier: 17231011

GI number: 17231011

Start: 4240062

End: 4242020

Strand: Reverse

Name: thiG [H]

Synonym: all3519

Alternate gene names: 17231011

Gene position: 4242020-4240062 (Counterclockwise)

Preceding gene: 17231012

Following gene: 17231010

Centisome position: 66.14

GC content: 48.95

Gene sequence:

>1959_bases
ATGACTAGGGACATTGTAATTATTGGTGGCGGCGTTATTGGTCTGGCGATCGCCGTTGAACTTAAATTGCGCGGGGCAGA
AGTCACCGTGATTTGTCGTGATTTCCAGGCTGCTGCTGCTCACGCCGCCGCCGGGATGTTAGCCCCCGATGCCGAACAAA
TCACAGATGGAGCGATGAAGTCGCTATGCTGGCGATCGCGTTCTTTATATTCCGAATGGACAAGCAAGTTAGAAGATTTA
ACGGGTTTAAACACTGGTTACTGGCCTTGTGGCATCCTAGCGCCAATTTATGAAGGGCAGGAGAGCAAGGGTGTTAGGGT
GCAGGAGGGTGAAGGAGAATCACCTGCTTATTGGTTAGAAAAAGCCGCTATTCATCAATACCAACCAGGGTTAGGTGAGG
ATGTGGTTGGTGGTTGGTGGTATCCAGAGGATGCCCAAGTGAATAACCAAGCACTAGCGCGTGTGCTATGGGCTGCGGCG
GAAAGCCTTGGTGTGGAACTTAAAGACGGAATTACAGTAGAAGGATTATTACAACAGCAGGGACAGGTAGTAGGTGTCCA
AACCAACACCGGCATCATTCGGGCTGAACACTATGTTTTAGCTACAGGTGCTTGGGCCAATGAATTATTACCCTTACCCG
TAACCCCTCGCAAAGGGCAAATGTTACGCCTGCGTGTGCCGGAGTCTGTACCGGAATTGCCTTTAAAGCGGGTTTTATTT
GGCAAAAATATTTACATTGTACCGAGACGTGAGCGCTCTATTATTGTTGGGGCAACAAGTGAAGATGTCGGCTTTACTCC
TCACAACACCCCCGCCGGCATTCAAACTTTACTCCAAGGCGCAATTCGTCTCTATCCTCAGTTACAGGACTATCCCATTC
AAGAGTTTTGGTGGGGCTTCCGTCCAGCCACTCCAGATGAATTACCTATACTAGGCACTAGTCACTGTCCCAATTTAACT
TTAGCTACTGGTCATTATCGTAACGGCATCTTGCTAGCACCAATAACCGCCGCACTTATAGCCGATTTAATCGTAGAACA
AAAATCTGACCCCCTACTGTCCCATTTCCACTATTCACGCAGCCAAAAACAGGCATCTACCATCCCCATGTTGACCCACT
CCGCCAACTTTTCTAACGGACACACCAAAAACCCCCCACTCCCCACTCTAGACTCACCCCTCATCATCGCAGGCAAATCC
TTTCATTCCCGTTTGATGACAGGGACAGGCAAATATCGCAGCATAGAAGAAATGCAGCAAAGTGTTGTTGCTAGCGGTTG
CGAAATTGTCACGGTGGCGGTGCGGCGAGTCCAAACCAAAACCCCAGGCCATGAAGGTTTAGCCGAAGCCCTGGACTGGT
CAAAAATTTGGATGTTGCCGAATACAGCTGGCTGTCAAACCGCAGAAGAAGCCATTCGTGTGGCTCGTTTGGGAAGAGAA
ATGGCTAAGTTATTAGGTCAAGAAGATAATAATTTTGTCAAGTTAGAAGTTATACCAGACCCTAAGTATTTACTTCCCGA
CCCCATTGGTACATTACAAGCCGCCGAACAGTTAGTGAAAGAAGGTTTCGCTGTCTTACCTTATATCAATGCCGACCCCA
TGCTAGCCAAGCATTTGGAAGATGTCGGCTGTGCTACAGTCATGCCATTAGCGTCACCCATTGGCTCAGGACAGGGTTTA
AAAACCACCGCCAACATTCAAATTATCATCGAAAATGCCAAGATCCCTGTAGTGGTAGATGCTGGCATTGGTGCGCCCTC
AGAAGCCTCCCAGGCGATGGAATTAGGGGCAGATGCCCTATTGATTAATAGTGCGATCGCCCTTGCTCAAAACCCAGCCG
CAATGGCTCAAGCCATGAACCTCGCAACAGTTGCCGGTCGTCTAGCCTACCTCGCAGGTAGAATGCCCATGAAAACCTAT
GCCAGTGCTAGCTCACCAGTCACAGGTACGATTAGTTAG

Upstream 100 bases:

>100_bases
TTTTGAATTCACCCCTACTTGTACTAGTCGTTGGTTACAAGTTATGAGTTATTAGTTATGTACTAATGGTAAATAGCTAA
TATATAAATACTAATGACTA

Downstream 100 bases:

>100_bases
TCAATAGTCAATAGTCAATAGTCCATATGGACTATTGACCAATAAGTAATGACAATTCACCCTTCGGGTGATGCTCGTTC
CTCTCTACGAGACGCTGCGC

Product: thiamin biosynthesis protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MTRDIVIIGGGVIGLAIAVELKLRGAEVTVICRDFQAAAAHAAAGMLAPDAEQITDGAMKSLCWRSRSLYSEWTSKLEDL
TGLNTGYWPCGILAPIYEGQESKGVRVQEGEGESPAYWLEKAAIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAA
ESLGVELKDGITVEGLLQQQGQVVGVQTNTGIIRAEHYVLATGAWANELLPLPVTPRKGQMLRLRVPESVPELPLKRVLF
GKNIYIVPRRERSIIVGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGFRPATPDELPILGTSHCPNLT
LATGHYRNGILLAPITAALIADLIVEQKSDPLLSHFHYSRSQKQASTIPMLTHSANFSNGHTKNPPLPTLDSPLIIAGKS
FHSRLMTGTGKYRSIEEMQQSVVASGCEIVTVAVRRVQTKTPGHEGLAEALDWSKIWMLPNTAGCQTAEEAIRVARLGRE
MAKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKHLEDVGCATVMPLASPIGSGQGL
KTTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADALLINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPMKTY
ASASSPVTGTIS

Sequences:

>Translated_652_residues
MTRDIVIIGGGVIGLAIAVELKLRGAEVTVICRDFQAAAAHAAAGMLAPDAEQITDGAMKSLCWRSRSLYSEWTSKLEDL
TGLNTGYWPCGILAPIYEGQESKGVRVQEGEGESPAYWLEKAAIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAA
ESLGVELKDGITVEGLLQQQGQVVGVQTNTGIIRAEHYVLATGAWANELLPLPVTPRKGQMLRLRVPESVPELPLKRVLF
GKNIYIVPRRERSIIVGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGFRPATPDELPILGTSHCPNLT
LATGHYRNGILLAPITAALIADLIVEQKSDPLLSHFHYSRSQKQASTIPMLTHSANFSNGHTKNPPLPTLDSPLIIAGKS
FHSRLMTGTGKYRSIEEMQQSVVASGCEIVTVAVRRVQTKTPGHEGLAEALDWSKIWMLPNTAGCQTAEEAIRVARLGRE
MAKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKHLEDVGCATVMPLASPIGSGQGL
KTTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADALLINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPMKTY
ASASSPVTGTIS
>Mature_651_residues
TRDIVIIGGGVIGLAIAVELKLRGAEVTVICRDFQAAAAHAAAGMLAPDAEQITDGAMKSLCWRSRSLYSEWTSKLEDLT
GLNTGYWPCGILAPIYEGQESKGVRVQEGEGESPAYWLEKAAIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAAE
SLGVELKDGITVEGLLQQQGQVVGVQTNTGIIRAEHYVLATGAWANELLPLPVTPRKGQMLRLRVPESVPELPLKRVLFG
KNIYIVPRRERSIIVGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGFRPATPDELPILGTSHCPNLTL
ATGHYRNGILLAPITAALIADLIVEQKSDPLLSHFHYSRSQKQASTIPMLTHSANFSNGHTKNPPLPTLDSPLIIAGKSF
HSRLMTGTGKYRSIEEMQQSVVASGCEIVTVAVRRVQTKTPGHEGLAEALDWSKIWMLPNTAGCQTAEEAIRVARLGREM
AKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKHLEDVGCATVMPLASPIGSGQGLK
TTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADALLINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPMKTYA
SASSPVTGTIS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=258, Percent_Identity=52.7131782945737, Blast_Score=252, Evalue=5e-68,
Organism=Escherichia coli, GI1787438, Length=398, Percent_Identity=27.1356783919598, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 70114; Mature: 69983

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDIVIIGGGVIGLAIAVELKLRGAEVTVICRDFQAAAAHAAAGMLAPDAEQITDGAMK
CCCEEEEECCCCEEEEEEEEEEECCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHH
SLCWRSRSLYSEWTSKLEDLTGLNTGYWPCGILAPIYEGQESKGVRVQEGEGESPAYWLE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCHHHHHH
KAAIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAAESLGVELKDGITVEGLLQQQ
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
GQVVGVQTNTGIIRAEHYVLATGAWANELLPLPVTPRKGQMLRLRVPESVPELPLKRVLF
CCEEEEEECCCEEEECEEEEEECCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHC
GKNIYIVPRRERSIIVGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGF
CCCEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCC
RPATPDELPILGTSHCPNLTLATGHYRNGILLAPITAALIADLIVEQKSDPLLSHFHYSR
CCCCCCCCCEEECCCCCCCEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SQKQASTIPMLTHSANFSNGHTKNPPLPTLDSPLIIAGKSFHSRLMTGTGKYRSIEEMQQ
HHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHH
SVVASGCEIVTVAVRRVQTKTPGHEGLAEALDWSKIWMLPNTAGCQTAEEAIRVARLGRE
HHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
MAKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKHLE
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHH
DVGCATVMPLASPIGSGQGLKTTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADAL
HCCCHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCHH
LINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPMKTYASASSPVTGTIS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEECCC
>Mature Secondary Structure 
TRDIVIIGGGVIGLAIAVELKLRGAEVTVICRDFQAAAAHAAAGMLAPDAEQITDGAMK
CCEEEEECCCCEEEEEEEEEEECCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHH
SLCWRSRSLYSEWTSKLEDLTGLNTGYWPCGILAPIYEGQESKGVRVQEGEGESPAYWLE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCHHHHHH
KAAIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAAESLGVELKDGITVEGLLQQQ
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
GQVVGVQTNTGIIRAEHYVLATGAWANELLPLPVTPRKGQMLRLRVPESVPELPLKRVLF
CCEEEEEECCCEEEECEEEEEECCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHC
GKNIYIVPRRERSIIVGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGF
CCCEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCC
RPATPDELPILGTSHCPNLTLATGHYRNGILLAPITAALIADLIVEQKSDPLLSHFHYSR
CCCCCCCCCEEECCCCCCCEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SQKQASTIPMLTHSANFSNGHTKNPPLPTLDSPLIIAGKSFHSRLMTGTGKYRSIEEMQQ
HHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHH
SVVASGCEIVTVAVRRVQTKTPGHEGLAEALDWSKIWMLPNTAGCQTAEEAIRVARLGRE
HHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
MAKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKHLE
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHH
DVGCATVMPLASPIGSGQGLKTTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADAL
HCCCHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCHH
LINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPMKTYASASSPVTGTIS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA