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The map label for this gene is trmE

Identifier: 170762183

GI number: 170762183

Start: 18380

End: 19696

Strand: Reverse

Name: trmE

Synonym: UPA3_0018

Alternate gene names: 170762183

Gene position: 19696-18380 (Counterclockwise)

Preceding gene: 170762264

Following gene: 170761942

Centisome position: 2.62

GC content: 25.59

Gene sequence:

>1317_bases
ATGTCAACAATTGTTGCTTTAGCAACCGCGCCTATGAATTGTGCCATTCACATTATTCGGGTTAGTGGTCCAAAAGCTTT
TGAAATAATTAATAAAATTTCAACAATAAAAATAAAAAAAGAAACTTTTAAAATTTGGTATACAATTCTAAAAGATGATA
ATCAAATTTTAGATGAAGTTTTAATAAATACATTTGTAGCTCCAAAAACATTTACTGGAGAAGATTTAGTAGAAATTAAT
TGCCACGGAGGAATAGTGGTTGCTAATTTAATTATTAAAACATTAATTAAATATGGTTGTCAACCAGCACAACGTGGAGA
ATTTTCTCGCCGTGCTTTATTAAATAAAAAAATGGATTTATCAAAAATTGAAGCAATTAACAATTTGGTTAATGCTAAAA
ATGAATTAAGCGTTAAAGGTGTAATTGGCGCTCTATTAGGACGTGTTTCGCAATCAATTGCTGAATTTAGGCATGAGTTA
TTTATGATAATTGGGCAAATTGAAGTTAATATTGATTATCCTGAATACGATGATGTTGAACAAGTTGATGCTATTATTTT
AAAACAACGTTTATTATCTTTAGATAAAAAAATTACAAAAATTATTGATCAATCAAAAAAATTTTTACCAATCAATAAAG
GCATTAGGGTGTTAATAATTGGAAAACCAAATGTAGGTAAATCAACTCTATTAAACGCTTTATGCAATGAACAAAAAGCG
ATTGTTACAGATATTCCTGGGACAACACGTGATGTTATTGAATCCTCAATTAATATTGATAATATTACTTTAAATATCTT
AGATACTGCAGGAATTCATTTAACAAATGATTTTGTTGAAAATTTAGGAATTAATAAAGCCAAAGCATTAATTGATAAAG
TTGATTTAATTTTATATTTAATACCTGCAAACGAACAACAAGATTTGGAGTTATATGATTTAATTAAAAAACAAAAACAT
TTATTAGTTTATACAAAAAAAGATTTAGTTGATCAATACGATGATAAACAAATATATATTAATGCAAAAAATAATGATAT
TCAGTCATTGATTGATGAAATTAAAAAACTTTTTTATGTTCAAGAGTTTGACAATGCTAACATTGATGTTTTACAATCCC
AACGTCAAATTGGTATTTTAGAAAATGTTCATTATTTAATTAATAACGCAATTCTTAATTTAGAAAAAGGGGATACACTT
GATTTAATTGTCGCTGATTTAGAATTTTGCAATTTACGTTTAAATGAACTCTTAGGCATAAGTAGCGAATATGATTTTTT
AGATGATTTATTTAAAAATTTTTGTATAGGTAAATAA

Upstream 100 bases:

>100_bases
TTTTGGATTTAATGCGTGCGAATTTAAAAATTAATATTAATAAAAATAATTTGTTTACTATTATTTATACAATTATTAAT
AAAAATAAAGGTGATTAACA

Downstream 100 bases:

>100_bases
TATGTTAGAAAAAATAAAAATTATTGATACTCATACTCATCCTAATATTGAACCATTAATTGAAGAATTTGATGACATTA
TCTTTAAATGTTATGAACAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSTIVALATAPMNCAIHIIRVSGPKAFEIINKISTIKIKKETFKIWYTILKDDNQILDEVLINTFVAPKTFTGEDLVEIN
CHGGIVVANLIIKTLIKYGCQPAQRGEFSRRALLNKKMDLSKIEAINNLVNAKNELSVKGVIGALLGRVSQSIAEFRHEL
FMIIGQIEVNIDYPEYDDVEQVDAIILKQRLLSLDKKITKIIDQSKKFLPINKGIRVLIIGKPNVGKSTLLNALCNEQKA
IVTDIPGTTRDVIESSINIDNITLNILDTAGIHLTNDFVENLGINKAKALIDKVDLILYLIPANEQQDLELYDLIKKQKH
LLVYTKKDLVDQYDDKQIYINAKNNDIQSLIDEIKKLFYVQEFDNANIDVLQSQRQIGILENVHYLINNAILNLEKGDTL
DLIVADLEFCNLRLNELLGISSEYDFLDDLFKNFCIGK

Sequences:

>Translated_438_residues
MSTIVALATAPMNCAIHIIRVSGPKAFEIINKISTIKIKKETFKIWYTILKDDNQILDEVLINTFVAPKTFTGEDLVEIN
CHGGIVVANLIIKTLIKYGCQPAQRGEFSRRALLNKKMDLSKIEAINNLVNAKNELSVKGVIGALLGRVSQSIAEFRHEL
FMIIGQIEVNIDYPEYDDVEQVDAIILKQRLLSLDKKITKIIDQSKKFLPINKGIRVLIIGKPNVGKSTLLNALCNEQKA
IVTDIPGTTRDVIESSINIDNITLNILDTAGIHLTNDFVENLGINKAKALIDKVDLILYLIPANEQQDLELYDLIKKQKH
LLVYTKKDLVDQYDDKQIYINAKNNDIQSLIDEIKKLFYVQEFDNANIDVLQSQRQIGILENVHYLINNAILNLEKGDTL
DLIVADLEFCNLRLNELLGISSEYDFLDDLFKNFCIGK
>Mature_437_residues
STIVALATAPMNCAIHIIRVSGPKAFEIINKISTIKIKKETFKIWYTILKDDNQILDEVLINTFVAPKTFTGEDLVEINC
HGGIVVANLIIKTLIKYGCQPAQRGEFSRRALLNKKMDLSKIEAINNLVNAKNELSVKGVIGALLGRVSQSIAEFRHELF
MIIGQIEVNIDYPEYDDVEQVDAIILKQRLLSLDKKITKIIDQSKKFLPINKGIRVLIIGKPNVGKSTLLNALCNEQKAI
VTDIPGTTRDVIESSINIDNITLNILDTAGIHLTNDFVENLGINKAKALIDKVDLILYLIPANEQQDLELYDLIKKQKHL
LVYTKKDLVDQYDDKQIYINAKNNDIQSLIDEIKKLFYVQEFDNANIDVLQSQRQIGILENVHYLINNAILNLEKGDTLD
LIVADLEFCNLRLNELLGISSEYDFLDDLFKNFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=25.8547008547009, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI193082993, Length=454, Percent_Identity=25.9911894273128, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=24.6, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI2367268, Length=458, Percent_Identity=30.3493449781659, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=36.0655737704918, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=458, Percent_Identity=30.5676855895196, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=29.4715447154472, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45550813, Length=477, Percent_Identity=28.9308176100629, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_UREP2 (B1AI04)

Other databases:

- EMBL:   CP000942
- RefSeq:   YP_001752098.1
- ProteinModelPortal:   B1AI04
- SMR:   B1AI04
- GeneID:   6155399
- GenomeReviews:   CP000942_GR
- KEGG:   upa:UPA3_0018
- HOGENOM:   HBG746795
- OMA:   VVEIACH
- ProtClustDB:   PRK05291
- BioCyc:   UPAR505682:UPA3_0018-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49464; Mature: 49333

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 78-78 BINDING 117-117 BINDING 438-438

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIVALATAPMNCAIHIIRVSGPKAFEIINKISTIKIKKETFKIWYTILKDDNQILDEV
CCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHEEEEHHHEEEEEEEECCCHHHHHHH
LINTFVAPKTFTGEDLVEINCHGGIVVANLIIKTLIKYGCQPAQRGEFSRRALLNKKMDL
HHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCH
SKIEAINNLVNAKNELSVKGVIGALLGRVSQSIAEFRHELFMIIGQIEVNIDYPEYDDVE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHH
QVDAIILKQRLLSLDKKITKIIDQSKKFLPINKGIRVLIIGKPNVGKSTLLNALCNEQKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCE
IVTDIPGTTRDVIESSINIDNITLNILDTAGIHLTNDFVENLGINKAKALIDKVDLILYL
EEECCCCCHHHHHHHCCCCCEEEEEEEECCCCEEEHHHHHHCCCCHHHHHHHHCCEEEEE
IPANEQQDLELYDLIKKQKHLLVYTKKDLVDQYDDKQIYINAKNNDIQSLIDEIKKLFYV
ECCCCCCCCHHHHHHHCCCEEEEEECHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
QEFDNANIDVLQSQRQIGILENVHYLINNAILNLEKGDTLDLIVADLEFCNLRLNELLGI
HHCCCCCHHHHHCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEHHHHHHHHHHHHHCC
SSEYDFLDDLFKNFCIGK
CCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STIVALATAPMNCAIHIIRVSGPKAFEIINKISTIKIKKETFKIWYTILKDDNQILDEV
CCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHEEEEHHHEEEEEEEECCCHHHHHHH
LINTFVAPKTFTGEDLVEINCHGGIVVANLIIKTLIKYGCQPAQRGEFSRRALLNKKMDL
HHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCH
SKIEAINNLVNAKNELSVKGVIGALLGRVSQSIAEFRHELFMIIGQIEVNIDYPEYDDVE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHH
QVDAIILKQRLLSLDKKITKIIDQSKKFLPINKGIRVLIIGKPNVGKSTLLNALCNEQKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCE
IVTDIPGTTRDVIESSINIDNITLNILDTAGIHLTNDFVENLGINKAKALIDKVDLILYL
EEECCCCCHHHHHHHCCCCCEEEEEEEECCCCEEEHHHHHHCCCCHHHHHHHHCCEEEEE
IPANEQQDLELYDLIKKQKHLLVYTKKDLVDQYDDKQIYINAKNNDIQSLIDEIKKLFYV
ECCCCCCCCHHHHHHHCCCEEEEEECHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
QEFDNANIDVLQSQRQIGILENVHYLINNAILNLEKGDTLDLIVADLEFCNLRLNELLGI
HHCCCCCHHHHHCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEHHHHHHHHHHHHHCC
SSEYDFLDDLFKNFCIGK
CCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA