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The map label for this gene is nirA [H]

Identifier: 170748717

GI number: 170748717

Start: 2431118

End: 2432935

Strand: Reverse

Name: nirA [H]

Synonym: Mrad2831_2299

Alternate gene names: 170748717

Gene position: 2432935-2431118 (Counterclockwise)

Preceding gene: 170748718

Following gene: 170748716

Centisome position: 40.03

GC content: 71.18

Gene sequence:

>1818_bases
ATGTCCGAGACCACCACCCAGAGCGCCGCGGGCGCGTTCGAGGCCGAGCAGAAGCGCTACCTCGAGGGCTTCGCCTCCGG
CATCGCCGCCGTCCGCCTGACCGGCGGCCTCGCCCCGGGCGGGGCCGGGGCGGCCGCGCCGCCCGCCGAGCCCACGGGCC
CGGACGCGATCCACATCAAGGCGCAGGACCGGACCGTCCGGGACGGCGGCAAGCTCTGCGATCAGGAGAAGTGGAAGCGC
GCCGAGAACCCGTTCGACGGCCACAATCGCCTCATCCGGGAGGCCGCCGCCGGCAAGCAGCCGAAGCCCGAGGACAATTT
CCGCTGGCGCTACCACGGGATGTTCTGGGTCGCGCCGAATCAGGTCTCGTACATGTGCCGGCTGCGGATCCCGAACGGGA
TCCTGCACCACTGGCAGTTCGCGGGCGTCGCCGACCTCGCCGACCAGCACGGCGGCGGCTACGTCCACGTGACCACCCGG
GCGAACCTGCAGGTGCGGGAGATCGATCCCGAGCACGCCCAGCCCTTCCTCGACGGGCTCACCGATATCGGCCTCACGGC
CCGGGGCGCGGGCGCCGACAACATCCGCAACGTCACCGGCTCCTCGACCGCCGGCATCGACGCCCAGGAGCTGCTGGACA
CGCGCCCGCTCGCCAAGTCCTGGCACAACTGGATCCTGAACGACCGCTCCCTCTACGGCCTGCCGCGCAAGTTCAACGTC
GCCTTCGACGGCAGCGGCGTCATGCCGACCCTGGAGGAGACGAACGACATCGGCTTCCAGGCCGTGGAGGTGCTGGACGG
GGCCGCCGTGCCGCCCGGCATCTACATGCGCCTCGTGCTCGGCGGCATCTCGGGCCACCGGGACCTCGCCCGCGACACCG
GCATCGTCCTGAAGCCGGAGGATTGCAACGCCGTCGCCGACGCGATCATCCGCGTCTTCATCGAGAACGGCGACCGGACG
AACCGCAACAAGAGCCGGATGAAGTACGTCCTCGACGCCTGGGGCTTCGACAAGTACCTCGCCGCCGTCGAGGACAAGCT
CGGCCGCAAGCTCGACCGGGTCGCGCCGGAGCACGTGGCCCCGCGCAAACCCACCGACCGCTTCGCCCATGTCGGCGTCC
ACGCCCAGAAGCAGCCCGGCCTCAACTGGATCGGCGTCGTCCTGCCGGTCGGCAAGATGACCAGCGATCAGGTCCGGGCG
CTGGCGAAGGTCGCGGCGGAGTGCGGCGACGGCCAGATCCGCCTCACCGTCTGGCAGAACTTCATCGTCTCCGGGGTTCC
GGACGCGAAGGTCGCCGAGGTGGAAGCCCGCGTGGAAGCGCTCGGCCTCACCACCAAGGCCGCGGGCATCCGCTCCGGCC
TCGTGGCCTGCACGGGCGCGCGGGGCTGCAAGTTCGCGGCCTCCGACACCAAGGGCCACGCCCTGATCATCGCCGACCAC
GTCGAGAAGGCCGTGCCGAACCTCGACGTGCCGGTGAACGTCCACCTCACCGGCTGCCATCACAGCTGCGCCCAGCACTA
CATCGGCGATATCGGGCTGATCGGCGCCAAGGTCGTGGTCTCGGAGGAGGGCGACACCGTCGAGGGCTACGACGTCGTGG
TCGGCGGCGGCTTCGCCGAGAACCCGAAGATCGGCACCGAGATCTGGAAGGCCGTGAAGGCCGAGGACGCGCCCGCCCGC
GTCGAGGCGCTGCTGCGCGCCTACCTCGCCCTCCGCCAGGGCCCCGAGGAGAGCTTCCAGGCCTTCACCAGCCGCACCGG
CGCGGAGGCGCTCCGCGACGCCGCCGAGGACCAGCCCGCCCTGAAGGCCGCCGCATGA

Upstream 100 bases:

>100_bases
TGCAAGGGCCTGATCCGCTCCGGTGCCCCGATCGAGAACGTGGACGACTTGATGTTCGGGCGCCCCGCGCCCGAGCCGCT
CGCTGCTTGAGGAGGCGCGG

Downstream 100 bases:

>100_bases
CCCAGCACGTCTCGCCCCCCCTCGTCCTGATCCCCGAGACCGCGCCCTTCAACGAGGACCAGCGCGCCTGGCTCTCGGGC
TACTTCGCGGCCCTGCTCGG

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MSETTTQSAAGAFEAEQKRYLEGFASGIAAVRLTGGLAPGGAGAAAPPAEPTGPDAIHIKAQDRTVRDGGKLCDQEKWKR
AENPFDGHNRLIREAAAGKQPKPEDNFRWRYHGMFWVAPNQVSYMCRLRIPNGILHHWQFAGVADLADQHGGGYVHVTTR
ANLQVREIDPEHAQPFLDGLTDIGLTARGAGADNIRNVTGSSTAGIDAQELLDTRPLAKSWHNWILNDRSLYGLPRKFNV
AFDGSGVMPTLEETNDIGFQAVEVLDGAAVPPGIYMRLVLGGISGHRDLARDTGIVLKPEDCNAVADAIIRVFIENGDRT
NRNKSRMKYVLDAWGFDKYLAAVEDKLGRKLDRVAPEHVAPRKPTDRFAHVGVHAQKQPGLNWIGVVLPVGKMTSDQVRA
LAKVAAECGDGQIRLTVWQNFIVSGVPDAKVAEVEARVEALGLTTKAAGIRSGLVACTGARGCKFAASDTKGHALIIADH
VEKAVPNLDVPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAENPKIGTEIWKAVKAEDAPAR
VEALLRAYLALRQGPEESFQAFTSRTGAEALRDAAEDQPALKAAA

Sequences:

>Translated_605_residues
MSETTTQSAAGAFEAEQKRYLEGFASGIAAVRLTGGLAPGGAGAAAPPAEPTGPDAIHIKAQDRTVRDGGKLCDQEKWKR
AENPFDGHNRLIREAAAGKQPKPEDNFRWRYHGMFWVAPNQVSYMCRLRIPNGILHHWQFAGVADLADQHGGGYVHVTTR
ANLQVREIDPEHAQPFLDGLTDIGLTARGAGADNIRNVTGSSTAGIDAQELLDTRPLAKSWHNWILNDRSLYGLPRKFNV
AFDGSGVMPTLEETNDIGFQAVEVLDGAAVPPGIYMRLVLGGISGHRDLARDTGIVLKPEDCNAVADAIIRVFIENGDRT
NRNKSRMKYVLDAWGFDKYLAAVEDKLGRKLDRVAPEHVAPRKPTDRFAHVGVHAQKQPGLNWIGVVLPVGKMTSDQVRA
LAKVAAECGDGQIRLTVWQNFIVSGVPDAKVAEVEARVEALGLTTKAAGIRSGLVACTGARGCKFAASDTKGHALIIADH
VEKAVPNLDVPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAENPKIGTEIWKAVKAEDAPAR
VEALLRAYLALRQGPEESFQAFTSRTGAEALRDAAEDQPALKAAA
>Mature_604_residues
SETTTQSAAGAFEAEQKRYLEGFASGIAAVRLTGGLAPGGAGAAAPPAEPTGPDAIHIKAQDRTVRDGGKLCDQEKWKRA
ENPFDGHNRLIREAAAGKQPKPEDNFRWRYHGMFWVAPNQVSYMCRLRIPNGILHHWQFAGVADLADQHGGGYVHVTTRA
NLQVREIDPEHAQPFLDGLTDIGLTARGAGADNIRNVTGSSTAGIDAQELLDTRPLAKSWHNWILNDRSLYGLPRKFNVA
FDGSGVMPTLEETNDIGFQAVEVLDGAAVPPGIYMRLVLGGISGHRDLARDTGIVLKPEDCNAVADAIIRVFIENGDRTN
RNKSRMKYVLDAWGFDKYLAAVEDKLGRKLDRVAPEHVAPRKPTDRFAHVGVHAQKQPGLNWIGVVLPVGKMTSDQVRAL
AKVAAECGDGQIRLTVWQNFIVSGVPDAKVAEVEARVEALGLTTKAAGIRSGLVACTGARGCKFAASDTKGHALIIADHV
EKAVPNLDVPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAENPKIGTEIWKAVKAEDAPARV
EALLRAYLALRQGPEESFQAFTSRTGAEALRDAAEDQPALKAAA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=573, Percent_Identity=23.7347294938918, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322597, Length=537, Percent_Identity=22.7188081936685, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 64945; Mature: 64814

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETTTQSAAGAFEAEQKRYLEGFASGIAAVRLTGGLAPGGAGAAAPPAEPTGPDAIHIK
CCCCCCCHHCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
AQDRTVRDGGKLCDQEKWKRAENPFDGHNRLIREAAAGKQPKPEDNFRWRYHGMFWVAPN
ECCCEECCCHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
QVSYMCRLRIPNGILHHWQFAGVADLADQHGGGYVHVTTRANLQVREIDPEHAQPFLDGL
CEEEEEEEECCCCHHHHHHHCCHHHHHHCCCCCEEEEEEECCEEEEECCHHHHHHHHHHH
TDIGLTARGAGADNIRNVTGSSTAGIDAQELLDTRPLAKSWHNWILNDRSLYGLPRKFNV
HHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEECCCCEEEE
AFDGSGVMPTLEETNDIGFQAVEVLDGAAVPPGIYMRLVLGGISGHRDLARDTGIVLKPE
EECCCCCCCCHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCEECCC
DCNAVADAIIRVFIENGDRTNRNKSRMKYVLDAWGFDKYLAAVEDKLGRKLDRVAPEHVA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHCC
PRKPTDRFAHVGVHAQKQPGLNWIGVVLPVGKMTSDQVRALAKVAAECGDGQIRLTVWQN
CCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEHH
FIVSGVPDAKVAEVEARVEALGLTTKAAGIRSGLVACTGARGCKFAASDTKGHALIIADH
HHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCEEECCCCCCCEEEEEHH
VEKAVPNLDVPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAE
HHHHCCCCCCCEEEEEECCHHHHHHHHCCCEEEECEEEEEECCCCCCCCEEEEEECCCCC
NPKIGTEIWKAVKAEDAPARVEALLRAYLALRQGPEESFQAFTSRTGAEALRDAAEDQPA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCC
LKAAA
CCCCC
>Mature Secondary Structure 
SETTTQSAAGAFEAEQKRYLEGFASGIAAVRLTGGLAPGGAGAAAPPAEPTGPDAIHIK
CCCCCCHHCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
AQDRTVRDGGKLCDQEKWKRAENPFDGHNRLIREAAAGKQPKPEDNFRWRYHGMFWVAPN
ECCCEECCCHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
QVSYMCRLRIPNGILHHWQFAGVADLADQHGGGYVHVTTRANLQVREIDPEHAQPFLDGL
CEEEEEEEECCCCHHHHHHHCCHHHHHHCCCCCEEEEEEECCEEEEECCHHHHHHHHHHH
TDIGLTARGAGADNIRNVTGSSTAGIDAQELLDTRPLAKSWHNWILNDRSLYGLPRKFNV
HHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEECCCCEEEE
AFDGSGVMPTLEETNDIGFQAVEVLDGAAVPPGIYMRLVLGGISGHRDLARDTGIVLKPE
EECCCCCCCCHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCEECCC
DCNAVADAIIRVFIENGDRTNRNKSRMKYVLDAWGFDKYLAAVEDKLGRKLDRVAPEHVA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHCC
PRKPTDRFAHVGVHAQKQPGLNWIGVVLPVGKMTSDQVRALAKVAAECGDGQIRLTVWQN
CCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEHH
FIVSGVPDAKVAEVEARVEALGLTTKAAGIRSGLVACTGARGCKFAASDTKGHALIIADH
HHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCEEECCCCCCCEEEEEHH
VEKAVPNLDVPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAE
HHHHCCCCCCCEEEEEECCHHHHHHHHCCCEEEECEEEEEECCCCCCCCEEEEEECCCCC
NPKIGTEIWKAVKAEDAPARVEALLRAYLALRQGPEESFQAFTSRTGAEALRDAAEDQPA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCC
LKAAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]