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The map label for this gene is nasD [H]

Identifier: 170748718

GI number: 170748718

Start: 2432946

End: 2434214

Strand: Reverse

Name: nasD [H]

Synonym: Mrad2831_2300

Alternate gene names: 170748718

Gene position: 2434214-2432946 (Counterclockwise)

Preceding gene: 170748719

Following gene: 170748717

Centisome position: 40.05

GC content: 73.84

Gene sequence:

>1269_bases
ATGGCGACATCCCCTAAGGACACACGCGGACACGACGCGCGCGCCGGGAACGGCCCGCCGCGCGAGAGGCTGGTCGTCGT
CGGCAACGGCATGGCCTCGCTGCGCTTCCTGGAGCGGCTGACCGAGGCCGCGCCCGGCCGCTACGACATCACCTGCATCG
GGGCCGAGCCCACGGCCGCCTACAACCGGGTCCTGCTCTCCTCGCTGCTCGGCGGCGAGGTCGACGAGGCGGCCTGCGCC
TTCCGGGGCCTCGACTGGTACGCGGATCACGGCATCCGCCTGATCACCGGCGCGCCCGTGACGCAGATCGATCGGGAGAA
CGGGCTCGTCATCGTGGGCGAGACCCACGTCGTGCCCTTCGACAGGCTGGTCCTCGCGGTCGGCTCCCTGCCGATCCGCC
TGCCCAAACCCGGCATGGACCTTCCGGGCGTCATCACCTTCCGCGACCTCGCCGACGTGGCGGCGATCCGCGCGGCGGCC
GTCCGGCACGCCCGGGCCGTCGTCATCGGCGGCGGCCTCCTGGGCCTCGAGGCGGCGGTCGGGCTCGCGCGCCTCGGGGT
CGAGACCACGCTGCTCCACGTCATGGACCGGCTGATGGAGCGCCAGCTCGATCACCACGCCGCCGGGCTGGTGAAGCGCG
CCATGGAGGCGCGCGGCGTGCGGGTCATCCTCGAGGCCGACACCGCCCGCGTCGAGGGCGCGTCCCGGGTCGAGCGCCTG
GTCCTGGCGGACGGCACCGCGCTCCCGGCCGACCTCGTGGTGATGTCGGTGGGCGTGCGCCCCTCGGTGGCGCTGGCCCA
GGAAGCGGGGCTGGCGATCGGCCGGGGGATCACGGTCGACGACCGGATGACGACCTCGGACCCGCGCATCTTCGCGCTCG
GCGAGTGCGCCGAGCACCGCGGCCAGATCTACGGCCTGGTCGAGCCCGCCTACGAGCACGCCGACACGCTCGCCCGCCAC
CTCGCCGGCGAGGCGGCCGAGTACCGGGGCACGGCCCTGTCCACGAGCCTGAAGGTGTCGGGCCTGCCGGTCTTCTCGGC
CGGGATCGTGGACACGCCCGACGATGCCGAGGCGATCGTGCTCTCCGACCCGGGTGCCGGCCTCTACCGCAAGCTGCTGG
TGCGCGACGGCCGACTGATCGGCGCGGTGTTCGTCGGCGACATCGCCGAGCAGGGCGCCTGCAAGGGCCTGATCCGCTCC
GGTGCCCCGATCGAGAACGTGGACGACTTGATGTTCGGGCGCCCCGCGCCCGAGCCGCTCGCTGCTTGA

Upstream 100 bases:

>100_bases
CTCCCTGAGGCTGAAGCCTGCCGGGAGATGACCGGCAGCGGCTTTTTCGTGGGCGGTCCGCCAACGACCCTGCAATCGAG
GTTCCAGACGCGATCGAGCG

Downstream 100 bases:

>100_bases
GGAGGCGCGGATGTCCGAGACCACCACCCAGAGCGCCGCGGGCGCGTTCGAGGCCGAGCAGAAGCGCTACCTCGAGGGCT
TCGCCTCCGGCATCGCCGCC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MATSPKDTRGHDARAGNGPPRERLVVVGNGMASLRFLERLTEAAPGRYDITCIGAEPTAAYNRVLLSSLLGGEVDEAACA
FRGLDWYADHGIRLITGAPVTQIDRENGLVIVGETHVVPFDRLVLAVGSLPIRLPKPGMDLPGVITFRDLADVAAIRAAA
VRHARAVVIGGGLLGLEAAVGLARLGVETTLLHVMDRLMERQLDHHAAGLVKRAMEARGVRVILEADTARVEGASRVERL
VLADGTALPADLVVMSVGVRPSVALAQEAGLAIGRGITVDDRMTTSDPRIFALGECAEHRGQIYGLVEPAYEHADTLARH
LAGEAAEYRGTALSTSLKVSGLPVFSAGIVDTPDDAEAIVLSDPGAGLYRKLLVRDGRLIGAVFVGDIAEQGACKGLIRS
GAPIENVDDLMFGRPAPEPLAA

Sequences:

>Translated_422_residues
MATSPKDTRGHDARAGNGPPRERLVVVGNGMASLRFLERLTEAAPGRYDITCIGAEPTAAYNRVLLSSLLGGEVDEAACA
FRGLDWYADHGIRLITGAPVTQIDRENGLVIVGETHVVPFDRLVLAVGSLPIRLPKPGMDLPGVITFRDLADVAAIRAAA
VRHARAVVIGGGLLGLEAAVGLARLGVETTLLHVMDRLMERQLDHHAAGLVKRAMEARGVRVILEADTARVEGASRVERL
VLADGTALPADLVVMSVGVRPSVALAQEAGLAIGRGITVDDRMTTSDPRIFALGECAEHRGQIYGLVEPAYEHADTLARH
LAGEAAEYRGTALSTSLKVSGLPVFSAGIVDTPDDAEAIVLSDPGAGLYRKLLVRDGRLIGAVFVGDIAEQGACKGLIRS
GAPIENVDDLMFGRPAPEPLAA
>Mature_421_residues
ATSPKDTRGHDARAGNGPPRERLVVVGNGMASLRFLERLTEAAPGRYDITCIGAEPTAAYNRVLLSSLLGGEVDEAACAF
RGLDWYADHGIRLITGAPVTQIDRENGLVIVGETHVVPFDRLVLAVGSLPIRLPKPGMDLPGVITFRDLADVAAIRAAAV
RHARAVVIGGGLLGLEAAVGLARLGVETTLLHVMDRLMERQLDHHAAGLVKRAMEARGVRVILEADTARVEGASRVERLV
LADGTALPADLVVMSVGVRPSVALAQEAGLAIGRGITVDDRMTTSDPRIFALGECAEHRGQIYGLVEPAYEHADTLARHL
AGEAAEYRGTALSTSLKVSGLPVFSAGIVDTPDDAEAIVLSDPGAGLYRKLLVRDGRLIGAVFVGDIAEQGACKGLIRSG
APIENVDDLMFGRPAPEPLAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=278, Percent_Identity=25.1798561151079, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI226437568, Length=278, Percent_Identity=25.1798561151079, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI65787454, Length=278, Percent_Identity=25.1798561151079, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI22202629, Length=191, Percent_Identity=27.2251308900524, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4757732, Length=191, Percent_Identity=27.2251308900524, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789765, Length=412, Percent_Identity=35.4368932038835, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789065, Length=333, Percent_Identity=27.027027027027, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788892, Length=215, Percent_Identity=27.4418604651163, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=304, Percent_Identity=24.0131578947368, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24639257, Length=338, Percent_Identity=26.3313609467456, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281359715, Length=338, Percent_Identity=26.3313609467456, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281359713, Length=338, Percent_Identity=26.3313609467456, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24639250, Length=338, Percent_Identity=26.3313609467456, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18543267, Length=338, Percent_Identity=26.3313609467456, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24639252, Length=338, Percent_Identity=26.3313609467456, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585130, Length=287, Percent_Identity=22.2996515679443, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28573993, Length=217, Percent_Identity=28.110599078341, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581020, Length=217, Percent_Identity=28.110599078341, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 44381; Mature: 44249

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSPKDTRGHDARAGNGPPRERLVVVGNGMASLRFLERLTEAAPGRYDITCIGAEPTAA
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
YNRVLLSSLLGGEVDEAACAFRGLDWYADHGIRLITGAPVTQIDRENGLVIVGETHVVPF
HHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCEEEEECCCCEEEECCCCEEEEECCCCCCH
DRLVLAVGSLPIRLPKPGMDLPGVITFRDLADVAAIRAAAVRHARAVVIGGGLLGLEAAV
HHHHHHHCCCCEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHH
GLARLGVETTLLHVMDRLMERQLDHHAAGLVKRAMEARGVRVILEADTARVEGASRVERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHEE
VLADGTALPADLVVMSVGVRPSVALAQEAGLAIGRGITVDDRMTTSDPRIFALGECAEHR
EEECCCCCCHHHEEEECCCCCCHHHHHHCCCEECCCCEECCCCCCCCCCEEEECHHHHCC
GQIYGLVEPAYEHADTLARHLAGEAAEYRGTALSTSLKVSGLPVFSAGIVDTPDDAEAIV
CCEEEEECHHHHHHHHHHHHHHCCHHHHCCCEEECEEEECCCCEEECCCCCCCCCCCEEE
LSDPGAGLYRKLLVRDGRLIGAVFVGDIAEQGACKGLIRSGAPIENVDDLMFGRPAPEPL
EECCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCC
AA
CC
>Mature Secondary Structure 
ATSPKDTRGHDARAGNGPPRERLVVVGNGMASLRFLERLTEAAPGRYDITCIGAEPTAA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
YNRVLLSSLLGGEVDEAACAFRGLDWYADHGIRLITGAPVTQIDRENGLVIVGETHVVPF
HHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCEEEEECCCCEEEECCCCEEEEECCCCCCH
DRLVLAVGSLPIRLPKPGMDLPGVITFRDLADVAAIRAAAVRHARAVVIGGGLLGLEAAV
HHHHHHHCCCCEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHH
GLARLGVETTLLHVMDRLMERQLDHHAAGLVKRAMEARGVRVILEADTARVEGASRVERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHEE
VLADGTALPADLVVMSVGVRPSVALAQEAGLAIGRGITVDDRMTTSDPRIFALGECAEHR
EEECCCCCCHHHEEEECCCCCCHHHHHHCCCEECCCCEECCCCCCCCCCEEEECHHHHCC
GQIYGLVEPAYEHADTLARHLAGEAAEYRGTALSTSLKVSGLPVFSAGIVDTPDDAEAIV
CCEEEEECHHHHHHHHHHHHHHCCHHHHCCCEEECEEEECCCCEEECCCCCCCCCCCEEE
LSDPGAGLYRKLLVRDGRLIGAVFVGDIAEQGACKGLIRSGAPIENVDDLMFGRPAPEPL
EECCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]