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The map label for this gene is nasA [H]

Identifier: 170748715

GI number: 170748715

Start: 2426732

End: 2429428

Strand: Reverse

Name: nasA [H]

Synonym: Mrad2831_2297

Alternate gene names: 170748715

Gene position: 2429428-2426732 (Counterclockwise)

Preceding gene: 170748716

Following gene: 170748714

Centisome position: 39.97

GC content: 73.45

Gene sequence:

>2697_bases
ATGACCCAGCCAGTGGCGGCGCCCACGGTGCGGACCACCTGCCCCTATTGCGGGGTCGGGTGCGGCGTGCTCGCGACCCC
GGACGGCCAGGGCGGCGCCACGATCGCGGGCGACGCCGAGCACCCGGCCAATTTCGGCCGCCTGTGCTCGAAGGGCTCGG
CGCTCGGCGAGACCCTCTCGCTGGAGAACCGCCTGCTCCACCCGCAGGTCGACGGCGCCCAGGTCTCCTGGGACGCCGCT
CTCGACACCGTCGCGAAGAAGCTCGGCGCGGTCGCCGCGGAGCACGGGCCGGACGCGATCGCCTTCTACCTGTCGGGGCA
GCTCCTCACCGAGGATTACTACGTCGCCAACAAGCTCGCGAAGGGCTTCATCGGCACGCCGCACGTGGACACCAACTCGC
GGCTCTGCATGTCGAGCGCGGTCGCGGCCCACCGCCGGGCCTTCGGCTCCGACACGGTGCCGGGCTGCTACGAGGATCTC
GACGAGGCCGACCTGATCGTGCTGGTCGGCTCCAACACCGCGTGGTGCCACCCGATCCTGTTCCGCCGCATGGCCGACGC
CAAGGAGCGCCGCGGCACCAAGTTCGTGGTCATCGATCCCCGCCGCACCCAGACCGCGCAGGAATCCGACCTGTTCCTCG
GCATCCGGCCCGGCTCCGACACCGCCCTGTTCGCGGGCCTGCTGGTCTACCTGTCCGACAACGGCTTCCGCGACCGCGCC
TTCGTCGATGCCCACACGGCCGGCCTCGACGGCGCCCTCGACCGCGCCCGCCAGATCGCGCCGGATCTCGCGGCGGTCGC
CGCCGTCACCGGGGTGGCGGAGGCCGACATCGCGACCTTCTACGCCCTGTTCGCCGGCACGCGCCGGGTCGTCACGGCCT
TCTCGCAGGGGGTGAACCAGTCGGCTCAGGGGACCGACAAGGGCAACAGCATCATCAACTGCCACCTCATCACCGGCCGC
ATCGGCGAGACCGGGATGGGGCCCTTCTCCCTGACCGGCCAGCCGAACGCCATGGGCGGGCGCGAGGTCGGGGGCTTGGC
CAACATGCTGGCCGCCCACATGCACTTCACTCCCGAGGAGGTGGACCGGGTGCGCCGCTACTGGGGCGCCCCGCGGATCA
TCACCGGCGAGGGGATGAAGGCGGTGGCGCTCTTCGATGCGATTGCCCGGGGCAAGATCAAGGCCCTCTGGGTCATGGGC
ACCAACCCGCTGGTCTCGATGCCGCGCGCCGACGCGATCCGCGCGTCGCTCGCCGGCCTCGACCTCTACGTGGTGTCCGA
GGCAGTGGCGGATTCCGACACCGCCCTGGGTCGCGGCAAGACCACAGTGCTGCTGCCTGCCCAGGCCTGGGGCGAGAAGG
ACGGCACCGTCACCAATTCCGAGCGCCGGATCTCGCGCCAGCGCCGCTTCCTCACGAGCCCGGGCGCGGCGCGGCCGGAC
TGGGCGGCGCTGGCCGAGGTCGGCCGCCGCCTCGGCCACGGCCGGGCCTTCGCGTTCGATTCCGCCGCGGCGATCTTCCG
CGAGCATGCCGGGCTCTCGGCCTACGAGAACAACGGCACCCGCGACTTCGACCTCGGCGCGCTCACCGACATCAGCGACG
CCGCCTACGACGCGGCCCCGCCGGTTCAGTGGCCGATCCCGAAACGCTCCGGCCTCCGGGGTGCCACCCAGGGCAAGGCT
CGTCTGTTCGGCGACGGCCGGTTCTACACCTTCGACCGGCGCGCCCGCTTCGTCGCGGTGCAGCCGCCGGCCCTCGCGCT
CGCGGCCTCGACGGCATTCCCGCTCATGCTCAACACCGGCCGGGTCCGCGACCACTGGCACACGATGACGCGCACCGGGA
AGAGCCAGCGCCTCTCGGCGCACCGGGCCGTCCCGTTCGTCGAGGTCCACCCGGACGACGCGGCGACCCACGGCTTGGTG
GAGGGCGACATCGCCCGGCTGACGACCGCGTACGGCGCGGCCGAGCTGGAAGTGACGGTCACCGACACGGTGGTGCCGGG
CAGCCTGTTCATGCCGATGCACTGGGGCGGCGCCTTCGCGTCGCGGGCCCGGGTCGGCGCGCTGGTGCGCGGCATGACCG
ACCCGGTCTCCGGTCAGCCCGAACTCAAGGCGACCCCGGCGGCCATCGCCTCCGTCGGCTACCGCGCCCGCGGTTTCCTG
CTGACGCGCCGGCAGGTGGCGATGCCCGAGGGCTGGTGGTGGGCGCGCGCCGCGATCACCGGCGGCTGGGGCGCGCAATT
CGCCACCGCCGACAGCTCCCGGGCGGTGGCGCTGACGGTCCGCGGGCTGATCCAGAGCGAGGCGCTGTCGGGCTGCGAAC
TGGCGGAATATGCCGACCACGCCCGCGACCGCTACCGCTGCGCGGTCTACGACGGCGACCGCCTCGTCGCCTGCCTCGCC
TACGCGCCCGCCGGGGAACGGCCGGACTGGGAAGCCGCCAAGGCCCTGTTCGCCGCCGAGGCGCCCGGCGCCGCCGAACG
ACGGGCGCTGCTCTCGGGCCGCGCGGCGACCGCCTCGGCGGGCCCCGTGGTCTGCGCCTGCCACGGCGTCGGCGCCGACG
TCATCGCCGCGACCATTGCCGGGGGCGCCGCATCCGTGGAGGCGGTGGGCGCAGCCTGCAAGGCCGGCACGAACTGCGGC
TCCTGCATACCGGAGATCCGCAAGATGCTGGCCGGGCAGACCGCCCGCGCCGCGTGA

Upstream 100 bases:

>100_bases
GACGTCTACTGAGCACCGGTAACCCTCCCCCCTGTGCCGGGGGAGGGCGATGCGGCGCGGCGCTTGCTCCCGCCCAGGAC
GACCCTTCGAGGATCCAGCC

Downstream 100 bases:

>100_bases
TTATCTGATACACTCACCGGCAAGGACGGACCGGGTGATCCCATCCGATCCCCTCATCCCGAGGTGACCGCGTCCGCGGG
CCTCGAAGGAGCCCTCCAGC

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 898; Mature: 897

Protein sequence:

>898_residues
MTQPVAAPTVRTTCPYCGVGCGVLATPDGQGGATIAGDAEHPANFGRLCSKGSALGETLSLENRLLHPQVDGAQVSWDAA
LDTVAKKLGAVAAEHGPDAIAFYLSGQLLTEDYYVANKLAKGFIGTPHVDTNSRLCMSSAVAAHRRAFGSDTVPGCYEDL
DEADLIVLVGSNTAWCHPILFRRMADAKERRGTKFVVIDPRRTQTAQESDLFLGIRPGSDTALFAGLLVYLSDNGFRDRA
FVDAHTAGLDGALDRARQIAPDLAAVAAVTGVAEADIATFYALFAGTRRVVTAFSQGVNQSAQGTDKGNSIINCHLITGR
IGETGMGPFSLTGQPNAMGGREVGGLANMLAAHMHFTPEEVDRVRRYWGAPRIITGEGMKAVALFDAIARGKIKALWVMG
TNPLVSMPRADAIRASLAGLDLYVVSEAVADSDTALGRGKTTVLLPAQAWGEKDGTVTNSERRISRQRRFLTSPGAARPD
WAALAEVGRRLGHGRAFAFDSAAAIFREHAGLSAYENNGTRDFDLGALTDISDAAYDAAPPVQWPIPKRSGLRGATQGKA
RLFGDGRFYTFDRRARFVAVQPPALALAASTAFPLMLNTGRVRDHWHTMTRTGKSQRLSAHRAVPFVEVHPDDAATHGLV
EGDIARLTTAYGAAELEVTVTDTVVPGSLFMPMHWGGAFASRARVGALVRGMTDPVSGQPELKATPAAIASVGYRARGFL
LTRRQVAMPEGWWWARAAITGGWGAQFATADSSRAVALTVRGLIQSEALSGCELAEYADHARDRYRCAVYDGDRLVACLA
YAPAGERPDWEAAKALFAAEAPGAAERRALLSGRAATASAGPVVCACHGVGADVIAATIAGGAASVEAVGAACKAGTNCG
SCIPEIRKMLAGQTARAA

Sequences:

>Translated_898_residues
MTQPVAAPTVRTTCPYCGVGCGVLATPDGQGGATIAGDAEHPANFGRLCSKGSALGETLSLENRLLHPQVDGAQVSWDAA
LDTVAKKLGAVAAEHGPDAIAFYLSGQLLTEDYYVANKLAKGFIGTPHVDTNSRLCMSSAVAAHRRAFGSDTVPGCYEDL
DEADLIVLVGSNTAWCHPILFRRMADAKERRGTKFVVIDPRRTQTAQESDLFLGIRPGSDTALFAGLLVYLSDNGFRDRA
FVDAHTAGLDGALDRARQIAPDLAAVAAVTGVAEADIATFYALFAGTRRVVTAFSQGVNQSAQGTDKGNSIINCHLITGR
IGETGMGPFSLTGQPNAMGGREVGGLANMLAAHMHFTPEEVDRVRRYWGAPRIITGEGMKAVALFDAIARGKIKALWVMG
TNPLVSMPRADAIRASLAGLDLYVVSEAVADSDTALGRGKTTVLLPAQAWGEKDGTVTNSERRISRQRRFLTSPGAARPD
WAALAEVGRRLGHGRAFAFDSAAAIFREHAGLSAYENNGTRDFDLGALTDISDAAYDAAPPVQWPIPKRSGLRGATQGKA
RLFGDGRFYTFDRRARFVAVQPPALALAASTAFPLMLNTGRVRDHWHTMTRTGKSQRLSAHRAVPFVEVHPDDAATHGLV
EGDIARLTTAYGAAELEVTVTDTVVPGSLFMPMHWGGAFASRARVGALVRGMTDPVSGQPELKATPAAIASVGYRARGFL
LTRRQVAMPEGWWWARAAITGGWGAQFATADSSRAVALTVRGLIQSEALSGCELAEYADHARDRYRCAVYDGDRLVACLA
YAPAGERPDWEAAKALFAAEAPGAAERRALLSGRAATASAGPVVCACHGVGADVIAATIAGGAASVEAVGAACKAGTNCG
SCIPEIRKMLAGQTARAA
>Mature_897_residues
TQPVAAPTVRTTCPYCGVGCGVLATPDGQGGATIAGDAEHPANFGRLCSKGSALGETLSLENRLLHPQVDGAQVSWDAAL
DTVAKKLGAVAAEHGPDAIAFYLSGQLLTEDYYVANKLAKGFIGTPHVDTNSRLCMSSAVAAHRRAFGSDTVPGCYEDLD
EADLIVLVGSNTAWCHPILFRRMADAKERRGTKFVVIDPRRTQTAQESDLFLGIRPGSDTALFAGLLVYLSDNGFRDRAF
VDAHTAGLDGALDRARQIAPDLAAVAAVTGVAEADIATFYALFAGTRRVVTAFSQGVNQSAQGTDKGNSIINCHLITGRI
GETGMGPFSLTGQPNAMGGREVGGLANMLAAHMHFTPEEVDRVRRYWGAPRIITGEGMKAVALFDAIARGKIKALWVMGT
NPLVSMPRADAIRASLAGLDLYVVSEAVADSDTALGRGKTTVLLPAQAWGEKDGTVTNSERRISRQRRFLTSPGAARPDW
AALAEVGRRLGHGRAFAFDSAAAIFREHAGLSAYENNGTRDFDLGALTDISDAAYDAAPPVQWPIPKRSGLRGATQGKAR
LFGDGRFYTFDRRARFVAVQPPALALAASTAFPLMLNTGRVRDHWHTMTRTGKSQRLSAHRAVPFVEVHPDDAATHGLVE
GDIARLTTAYGAAELEVTVTDTVVPGSLFMPMHWGGAFASRARVGALVRGMTDPVSGQPELKATPAAIASVGYRARGFLL
TRRQVAMPEGWWWARAAITGGWGAQFATADSSRAVALTVRGLIQSEALSGCELAEYADHARDRYRCAVYDGDRLVACLAY
APAGERPDWEAAKALFAAEAPGAAERRALLSGRAATASAGPVVCACHGVGADVIAATIAGGAASVEAVGAACKAGTNCGS
CIPEIRKMLAGQTARAA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=804, Percent_Identity=27.7363184079602, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI3868721, Length=714, Percent_Identity=26.4705882352941, Blast_Score=241, Evalue=2e-64,
Organism=Escherichia coli, GI3868719, Length=621, Percent_Identity=23.5104669887279, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI3868720, Length=262, Percent_Identity=33.206106870229, Blast_Score=140, Evalue=5e-34,
Organism=Escherichia coli, GI1787778, Length=408, Percent_Identity=24.5098039215686, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1787870, Length=233, Percent_Identity=25.3218884120172, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 94553; Mature: 94422

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPVAAPTVRTTCPYCGVGCGVLATPDGQGGATIAGDAEHPANFGRLCSKGSALGETLS
CCCCCCCCCHHCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCHHHHHHHCCCHHHHHHH
LENRLLHPQVDGAQVSWDAALDTVAKKLGAVAAEHGPDAIAFYLSGQLLTEDYYVANKLA
HHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECEEEECHHHHHHHHH
KGFIGTPHVDTNSRLCMSSAVAAHRRAFGSDTVPGCYEDLDEADLIVLVGSNTAWCHPIL
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECCCCCHHHHHH
FRRMADAKERRGTKFVVIDPRRTQTAQESDLFLGIRPGSDTALFAGLLVYLSDNGFRDRA
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHEEHEEECCCCCCCCE
FVDAHTAGLDGALDRARQIAPDLAAVAAVTGVAEADIATFYALFAGTRRVVTAFSQGVNQ
EEEHHCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHCCCC
SAQGTDKGNSIINCHLITGRIGETGMGPFSLTGQPNAMGGREVGGLANMLAAHMHFTPEE
CCCCCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHH
VDRVRRYWGAPRIITGEGMKAVALFDAIARGKIKALWVMGTNPLVSMPRADAIRASLAGL
HHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCC
DLYVVSEAVADSDTALGRGKTTVLLPAQAWGEKDGTVTNSERRISRQRRFLTSPGAARPD
EEEEEHHHHCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
WAALAEVGRRLGHGRAFAFDSAAAIFREHAGLSAYENNGTRDFDLGALTDISDAAYDAAP
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCEECCCCCCEECCCCCCCCCHHHCCCCC
PVQWPIPKRSGLRGATQGKARLFGDGRFYTFDRRARFVAVQPPALALAASTAFPLMLNTG
CCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCEEEEEECCCHHEEECCCCCEEEEECC
RVRDHWHTMTRTGKSQRLSAHRAVPFVEVHPDDAATHGLVEGDIARLTTAYGAAELEVTV
CCHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCHHHCCCCCCHHHHHHHHCCCEEEEEEE
TDTVVPGSLFMPMHWGGAFASRARVGALVRGMTDPVSGQPELKATPAAIASVGYRARGFL
ECEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEE
LTRRQVAMPEGWWWARAAITGGWGAQFATADSSRAVALTVRGLIQSEALSGCELAEYADH
EECHHHCCCCCCEEEEEEEECCCCCEEEECCCCCEEEEEEHHHHHHHHCCCCHHHHHHHH
ARDRYRCAVYDGDRLVACLAYAPAGERPDWEAAKALFAAEAPGAAERRALLSGRAATASA
HCCCEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
GPVVCACHGVGADVIAATIAGGAASVEAVGAACKAGTNCGSCIPEIRKMLAGQTARAA
CCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQPVAAPTVRTTCPYCGVGCGVLATPDGQGGATIAGDAEHPANFGRLCSKGSALGETLS
CCCCCCCCHHCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCHHHHHHHCCCHHHHHHH
LENRLLHPQVDGAQVSWDAALDTVAKKLGAVAAEHGPDAIAFYLSGQLLTEDYYVANKLA
HHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECEEEECHHHHHHHHH
KGFIGTPHVDTNSRLCMSSAVAAHRRAFGSDTVPGCYEDLDEADLIVLVGSNTAWCHPIL
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECCCCCHHHHHH
FRRMADAKERRGTKFVVIDPRRTQTAQESDLFLGIRPGSDTALFAGLLVYLSDNGFRDRA
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHEEHEEECCCCCCCCE
FVDAHTAGLDGALDRARQIAPDLAAVAAVTGVAEADIATFYALFAGTRRVVTAFSQGVNQ
EEEHHCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHCCCC
SAQGTDKGNSIINCHLITGRIGETGMGPFSLTGQPNAMGGREVGGLANMLAAHMHFTPEE
CCCCCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHH
VDRVRRYWGAPRIITGEGMKAVALFDAIARGKIKALWVMGTNPLVSMPRADAIRASLAGL
HHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCC
DLYVVSEAVADSDTALGRGKTTVLLPAQAWGEKDGTVTNSERRISRQRRFLTSPGAARPD
EEEEEHHHHCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
WAALAEVGRRLGHGRAFAFDSAAAIFREHAGLSAYENNGTRDFDLGALTDISDAAYDAAP
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCEECCCCCCEECCCCCCCCCHHHCCCCC
PVQWPIPKRSGLRGATQGKARLFGDGRFYTFDRRARFVAVQPPALALAASTAFPLMLNTG
CCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCEEEEEECCCHHEEECCCCCEEEEECC
RVRDHWHTMTRTGKSQRLSAHRAVPFVEVHPDDAATHGLVEGDIARLTTAYGAAELEVTV
CCHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCHHHCCCCCCHHHHHHHHCCCEEEEEEE
TDTVVPGSLFMPMHWGGAFASRARVGALVRGMTDPVSGQPELKATPAAIASVGYRARGFL
ECEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEE
LTRRQVAMPEGWWWARAAITGGWGAQFATADSSRAVALTVRGLIQSEALSGCELAEYADH
EECHHHCCCCCCEEEEEEEECCCCCEEEECCCCCEEEEEEHHHHHHHHCCCCHHHHHHHH
ARDRYRCAVYDGDRLVACLAYAPAGERPDWEAAKALFAAEAPGAAERRALLSGRAATASA
HCCCEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
GPVVCACHGVGADVIAATIAGGAASVEAVGAACKAGTNCGSCIPEIRKMLAGQTARAA
CCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]