Click here to switch to the map view.

The map label for this gene is cysG [H]

Identifier: 170748714

GI number: 170748714

Start: 2425056

End: 2426528

Strand: Reverse

Name: cysG [H]

Synonym: Mrad2831_2296

Alternate gene names: 170748714

Gene position: 2426528-2425056 (Counterclockwise)

Preceding gene: 170748715

Following gene: 170748708

Centisome position: 39.92

GC content: 76.1

Gene sequence:

>1473_bases
GTGACCGAGACCCCCCTGCGCGCAGCCCGCGCCCCCCGCGAGACGAAGGCCGCCGGCCTGGAGCCGCTGGCGGTGCTGCC
CGTCTTCGTGCCCCTCCGCGGCAAGCGGGTCGTCCTCGCCGGCGCGTCGGGCGGCGCCCCCTGGAAGGTCAAGGTCCTCG
CCGCGGCCGGCGCCCACGTCGACGTCTACGCGCCCGAGCCCGGCGAGGAGCTGCGCGCCGTGCCGGGCGAGATCGTCGAC
GGGCGGGTGGAGCTGCACGAACGCGCCTGGAGCCCGGACGACCTCTGGGGCGCGGCCTTCTGCATCGGCGCGATGGAGGA
CGAAACCGAGTGCGTCGCCTTCGTGGCGGCCGCCCGCGGGGCCGGCGCCATCGTCAACGCGGTGGACCGGCCGCACCTCT
GCGACGTGAAGTTCGGCGCCATCGTCAACCGCTCGCCGCTCGTGGTCGGCATCTCGACCGAGGGCGCCGCCCCGGTCTTC
GGCCAGACCGTGCGTGCCCGGATCGAGGCGATGCTGCCCCGCGGGTTCGCCCGCTGGGTCGCGGCCGCCCGCGACTGGCG
CGACGGCGTCACCGCTCGCTTCCACGGCTTCGCCGACCGGCGCCTCTTCTGGGAGCGCTTCACCGACCGGGCCTTCGCGG
AGCCCGACCGGGCGCCGTCGGAGCGGGATCTCGCCGAGCTGCTCGGCGAGACCGAGACCGCCCCGAAGGGCGGCGCCGTG
ACGCTGGTCGGGGCCGGCCCCGGCGACGCGGAGCTCCTCACGCTGAAGGCGCTGCGGGCGCTGCGCAACGCCGACGTCAT
CCTCTACGACGACCTCGTCGCCCCCGAGATCCTCGACTACGCCCGCCGCGAGGCCCGCACCATGCTGGTCGGCAAGACCG
GCCACGGCCCGTCCTGCCGCCAGGACGACATCAACGCGCTGATGGTGCAGCTCGCCCGCTCCGGCAAGCAGGTGGTGCGG
CTGAAATCCGGCGACCCGCTGGTCTTCGGCCGGGCGGGCGAGGAGATCGAGGCCTGCCGGGCCGCCGGCATCCCGGTGAC
CGTGGTTCCGGGCGTCTCGGCCGCGCAGGGTGCGGCGGCGTCCCTGGGCCTGTCGCTGACCCATCGCGACGCCGCGCGCC
GCCTGCAATTCGTCACCGGCCACGACCGCCGCGGCGCTCTGCCGGACGATCTGAACTGGGGCGCGCTCGCCGATCCGAGC
GTCACCACGGTGGTCTACATGCCGAAGCGGACCCTGCGGGCGCTGCTCGCACGCGCCGTGGCGGAGGGCCTGGCGCCCGC
GACGCCGGCGCTGCTGGTGTTCAACGCCACCCGCCCGAACCAGCAGGCGCTGCGCGGCACGGCCGCGGACCTCGCCGACC
GGGTCGAGGCGGCCGGCCTCGACGGTCCGGCGCTCCTGATGGTGGGCGAGGCCTTCTCGGAGATGCGGGGCGCGCTCCCG
GCCCAGGCCGAGCAGAGCCGCGTCGCGGTCTGA

Upstream 100 bases:

>100_bases
CCGCGCGATCCCTGGAGCCTTCCTTCGAGGCTGCTGCGCGGCTCCTCGGGGCGAGGGGGCGGACGGGAGTGGCCGGCTCG
CGACGATCCAGGACAGGCCC

Downstream 100 bases:

>100_bases
GGCCGGATGGCGTGGGCGGCGTCGTCCGCCCGCCGGACGGCATGCGCCACGCCCTCCGGCCCCCGGGCGTGCTTCAAGTC
AGCGGCTGTGGCGCATTCGG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTETPLRAARAPRETKAAGLEPLAVLPVFVPLRGKRVVLAGASGGAPWKVKVLAAAGAHVDVYAPEPGEELRAVPGEIVD
GRVELHERAWSPDDLWGAAFCIGAMEDETECVAFVAAARGAGAIVNAVDRPHLCDVKFGAIVNRSPLVVGISTEGAAPVF
GQTVRARIEAMLPRGFARWVAAARDWRDGVTARFHGFADRRLFWERFTDRAFAEPDRAPSERDLAELLGETETAPKGGAV
TLVGAGPGDAELLTLKALRALRNADVILYDDLVAPEILDYARREARTMLVGKTGHGPSCRQDDINALMVQLARSGKQVVR
LKSGDPLVFGRAGEEIEACRAAGIPVTVVPGVSAAQGAAASLGLSLTHRDAARRLQFVTGHDRRGALPDDLNWGALADPS
VTTVVYMPKRTLRALLARAVAEGLAPATPALLVFNATRPNQQALRGTAADLADRVEAAGLDGPALLMVGEAFSEMRGALP
AQAEQSRVAV

Sequences:

>Translated_490_residues
MTETPLRAARAPRETKAAGLEPLAVLPVFVPLRGKRVVLAGASGGAPWKVKVLAAAGAHVDVYAPEPGEELRAVPGEIVD
GRVELHERAWSPDDLWGAAFCIGAMEDETECVAFVAAARGAGAIVNAVDRPHLCDVKFGAIVNRSPLVVGISTEGAAPVF
GQTVRARIEAMLPRGFARWVAAARDWRDGVTARFHGFADRRLFWERFTDRAFAEPDRAPSERDLAELLGETETAPKGGAV
TLVGAGPGDAELLTLKALRALRNADVILYDDLVAPEILDYARREARTMLVGKTGHGPSCRQDDINALMVQLARSGKQVVR
LKSGDPLVFGRAGEEIEACRAAGIPVTVVPGVSAAQGAAASLGLSLTHRDAARRLQFVTGHDRRGALPDDLNWGALADPS
VTTVVYMPKRTLRALLARAVAEGLAPATPALLVFNATRPNQQALRGTAADLADRVEAAGLDGPALLMVGEAFSEMRGALP
AQAEQSRVAV
>Mature_489_residues
TETPLRAARAPRETKAAGLEPLAVLPVFVPLRGKRVVLAGASGGAPWKVKVLAAAGAHVDVYAPEPGEELRAVPGEIVDG
RVELHERAWSPDDLWGAAFCIGAMEDETECVAFVAAARGAGAIVNAVDRPHLCDVKFGAIVNRSPLVVGISTEGAAPVFG
QTVRARIEAMLPRGFARWVAAARDWRDGVTARFHGFADRRLFWERFTDRAFAEPDRAPSERDLAELLGETETAPKGGAVT
LVGAGPGDAELLTLKALRALRNADVILYDDLVAPEILDYARREARTMLVGKTGHGPSCRQDDINALMVQLARSGKQVVRL
KSGDPLVFGRAGEEIEACRAAGIPVTVVPGVSAAQGAAASLGLSLTHRDAARRLQFVTGHDRRGALPDDLNWGALADPSV
TTVVYMPKRTLRALLARAVAEGLAPATPALLVFNATRPNQQALRGTAADLADRVEAAGLDGPALLMVGEAFSEMRGALPA
QAEQSRVAV

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=447, Percent_Identity=33.7807606263982, Blast_Score=238, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6322922, Length=247, Percent_Identity=32.3886639676113, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 51804; Mature: 51673

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETPLRAARAPRETKAAGLEPLAVLPVFVPLRGKRVVLAGASGGAPWKVKVLAAAGAHV
CCCCCCHHHCCCCHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCCEEEEEEEECCCEE
DVYAPEPGEELRAVPGEIVDGRVELHERAWSPDDLWGAAFCIGAMEDETECVAFVAAARG
EEECCCCCHHHHCCCCHHHCCCHHHHHHCCCCCHHHHHHHEECCCCCCHHHHHHHHHHCC
AGAIVNAVDRPHLCDVKFGAIVNRSPLVVGISTEGAAPVFGQTVRARIEAMLPRGFARWV
CCHHHHCCCCCCCCCEECCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
AAARDWRDGVTARFHGFADRRLFWERFTDRAFAEPDRAPSERDLAELLGETETAPKGGAV
HHHHHHHCCCCEEECCCHHHHHHHHHHHCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEE
TLVGAGPGDAELLTLKALRALRNADVILYDDLVAPEILDYARREARTMLVGKTGHGPSCR
EEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCCC
QDDINALMVQLARSGKQVVRLKSGDPLVFGRAGEEIEACRAAGIPVTVVPGVSAAQGAAA
HHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCEEEECCCCCCCCCHH
SLGLSLTHRDAARRLQFVTGHDRRGALPDDLNWGALADPSVTTVVYMPKRTLRALLARAV
HHCEEEHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHH
AEGLAPATPALLVFNATRPNQQALRGTAADLADRVEAAGLDGPALLMVGEAFSEMRGALP
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCC
AQAEQSRVAV
CCCCCCCCCC
>Mature Secondary Structure 
TETPLRAARAPRETKAAGLEPLAVLPVFVPLRGKRVVLAGASGGAPWKVKVLAAAGAHV
CCCCCHHHCCCCHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCCEEEEEEEECCCEE
DVYAPEPGEELRAVPGEIVDGRVELHERAWSPDDLWGAAFCIGAMEDETECVAFVAAARG
EEECCCCCHHHHCCCCHHHCCCHHHHHHCCCCCHHHHHHHEECCCCCCHHHHHHHHHHCC
AGAIVNAVDRPHLCDVKFGAIVNRSPLVVGISTEGAAPVFGQTVRARIEAMLPRGFARWV
CCHHHHCCCCCCCCCEECCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
AAARDWRDGVTARFHGFADRRLFWERFTDRAFAEPDRAPSERDLAELLGETETAPKGGAV
HHHHHHHCCCCEEECCCHHHHHHHHHHHCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEE
TLVGAGPGDAELLTLKALRALRNADVILYDDLVAPEILDYARREARTMLVGKTGHGPSCR
EEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCCC
QDDINALMVQLARSGKQVVRLKSGDPLVFGRAGEEIEACRAAGIPVTVVPGVSAAQGAAA
HHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCEEEECCCCCCCCCHH
SLGLSLTHRDAARRLQFVTGHDRRGALPDDLNWGALADPSVTTVVYMPKRTLRALLARAV
HHCEEEHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHH
AEGLAPATPALLVFNATRPNQQALRGTAADLADRVEAAGLDGPALLMVGEAFSEMRGALP
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCC
AQAEQSRVAV
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA