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The map label for this gene is glpK

Identifier: 170748596

GI number: 170748596

Start: 2319444

End: 2320949

Strand: Reverse

Name: glpK

Synonym: Mrad2831_2178

Alternate gene names: 170748596

Gene position: 2320949-2319444 (Counterclockwise)

Preceding gene: 170748597

Following gene: 170748595

Centisome position: 38.19

GC content: 69.59

Gene sequence:

>1506_bases
ATGAGCCGTTACGTCGGGGCCATCGACCAGGGTACCACCAGCAGCCGCTTCATCGTGTTCGACCGGGAGGGCAGCGTCAT
CGCGCTCGCCCAGGCGGAGCACGCGCAGATCTACCCGGCCCCCGGCCATGTCGAGCACGACGCCACGGAGATCTGGACGA
AGACGCAGAGCGTGATGCGCGAGGCGCTGGAGAAGGGCGGGCTCCAGCCCTCCGACCTCGCGGCGGTCGGCATCACGAAC
CAGCGCGAGACCACGCTGATCTGGGATCGGAAGACCGGCAAGCCCCTGCACAACGCCCTGGTCTGGCAGGACACCCGCAA
CGACCGCCTGGTGGCCGAGTTCGCCCGCGACGGCGGCCGCGACCGGTTCCGCGACCTCACCGGCCTGCCGCTGGCGAGCT
ACTTCTCCGGGCTCAAGCTCCGCTGGCTGCTCGACCATGTCGCGGGTGCCCGCGCGAAGGCCGAGGCCGGCGACGTGCTG
TTCGGCAACATCGACACGTGGCTCGTCTGGAACCTCACCGGCGGCACCGAGGGCGGCCTCCACGTCACCGACGTGACCAA
CGCCAGCCGCACGCAGCTCATGTCGCTCAAGACCCTGGAATGGGACGAGGGCATGCTCCGGACCTTCGGCATCCCGAAGG
CGATGCTGCCGAAGATCGTCTCGTCGAGCGAGGTCTACGGCGAGACCCGGGCACCGTTCGCGGGCGTGCCGATCGCCGGC
ATCCTCGGCGACCAGCAGGCGGCCCTGTTCGGCCAGACCTGCTTCGCGCCGGGCGAGGCCAAGAACACCTACGGCACCGG
TTGCTTCGCGCTGATGAACACCGGCGAGGAGCCGGTCCCGTCGAAGGCCGGCCTCGTCACCACGCTGGCTTACCGCCTCG
ACGGCCAGAAGCCGGCCTACGCGCTGGAGGGTTCGATCGCCATCACGGGCGCCCTGGTGCAGTGGCTGCGCGACAACCTG
CACATGATCAAGGATTCCGCCGAGGTCGAGACGCTCGCCACCACGGTCGAGGACAACGGCGGCGTCTACTTCGTGCCGGC
CTTCTCGGGCCTCTACGCGCCGCACTGGAACGAGGGCGCCCGGGGCCTGATCATCGGCCTCACCCGCTACGTCAACCGCG
GCCACATCGCCCGCTCGGTGCTGGAGGCGACCGCCTTCCAGACCCACGAGGTGCTGGACGCGATGGCCAAGGATTCCGGC
ATCCCCGTCAAGGAGTTGCGCGCCGACGGCGGCATGGTCGCGAACAACACGCTGATGCAGTTCCAGGCCGACATGCTCGA
CGTGCCGGTGGTGCGCCCGAAGGTGGCCGAGACCACCGCGCTCGGCGCCGCCTACGCGGCAGGTCTTGCGGTCGGCTACT
GGAAGGGGCTCGACGACCTCAAGCGCAACTGGGGCGTCGACAAGCGCTGGACGCCGAAGATGGATGCCGGCCGGCGCGAG
ACCATCGCCGCCGCCTGGAGCCGGGCCGTGCAGCGCTCCTTCGACTGGAAGACGGACGAGGATTGA

Upstream 100 bases:

>100_bases
GCGCGCCTCGCCGCCTACCTCGACCGCAGGGCCGAGGCCGCCTGATGCTTTCGAACCCGATCCGGCGGCAGACCGGTCGG
GCCTATGAAGGGAGGGAACG

Downstream 100 bases:

>100_bases
GGGACGGCCGTCAGGCCCGCGCGCGGCGTGCGGGCCTGACGGCCTCGTGACCACCTCCATCCCACCCACGCCCTCGTCCT
GAGGTGCGACGGAGCCTCCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSRYVGAIDQGTTSSRFIVFDREGSVIALAQAEHAQIYPAPGHVEHDATEIWTKTQSVMREALEKGGLQPSDLAAVGITN
QRETTLIWDRKTGKPLHNALVWQDTRNDRLVAEFARDGGRDRFRDLTGLPLASYFSGLKLRWLLDHVAGARAKAEAGDVL
FGNIDTWLVWNLTGGTEGGLHVTDVTNASRTQLMSLKTLEWDEGMLRTFGIPKAMLPKIVSSSEVYGETRAPFAGVPIAG
ILGDQQAALFGQTCFAPGEAKNTYGTGCFALMNTGEEPVPSKAGLVTTLAYRLDGQKPAYALEGSIAITGALVQWLRDNL
HMIKDSAEVETLATTVEDNGGVYFVPAFSGLYAPHWNEGARGLIIGLTRYVNRGHIARSVLEATAFQTHEVLDAMAKDSG
IPVKELRADGGMVANNTLMQFQADMLDVPVVRPKVAETTALGAAYAAGLAVGYWKGLDDLKRNWGVDKRWTPKMDAGRRE
TIAAAWSRAVQRSFDWKTDED

Sequences:

>Translated_501_residues
MSRYVGAIDQGTTSSRFIVFDREGSVIALAQAEHAQIYPAPGHVEHDATEIWTKTQSVMREALEKGGLQPSDLAAVGITN
QRETTLIWDRKTGKPLHNALVWQDTRNDRLVAEFARDGGRDRFRDLTGLPLASYFSGLKLRWLLDHVAGARAKAEAGDVL
FGNIDTWLVWNLTGGTEGGLHVTDVTNASRTQLMSLKTLEWDEGMLRTFGIPKAMLPKIVSSSEVYGETRAPFAGVPIAG
ILGDQQAALFGQTCFAPGEAKNTYGTGCFALMNTGEEPVPSKAGLVTTLAYRLDGQKPAYALEGSIAITGALVQWLRDNL
HMIKDSAEVETLATTVEDNGGVYFVPAFSGLYAPHWNEGARGLIIGLTRYVNRGHIARSVLEATAFQTHEVLDAMAKDSG
IPVKELRADGGMVANNTLMQFQADMLDVPVVRPKVAETTALGAAYAAGLAVGYWKGLDDLKRNWGVDKRWTPKMDAGRRE
TIAAAWSRAVQRSFDWKTDED
>Mature_500_residues
SRYVGAIDQGTTSSRFIVFDREGSVIALAQAEHAQIYPAPGHVEHDATEIWTKTQSVMREALEKGGLQPSDLAAVGITNQ
RETTLIWDRKTGKPLHNALVWQDTRNDRLVAEFARDGGRDRFRDLTGLPLASYFSGLKLRWLLDHVAGARAKAEAGDVLF
GNIDTWLVWNLTGGTEGGLHVTDVTNASRTQLMSLKTLEWDEGMLRTFGIPKAMLPKIVSSSEVYGETRAPFAGVPIAGI
LGDQQAALFGQTCFAPGEAKNTYGTGCFALMNTGEEPVPSKAGLVTTLAYRLDGQKPAYALEGSIAITGALVQWLRDNLH
MIKDSAEVETLATTVEDNGGVYFVPAFSGLYAPHWNEGARGLIIGLTRYVNRGHIARSVLEATAFQTHEVLDAMAKDSGI
PVKELRADGGMVANNTLMQFQADMLDVPVVRPKVAETTALGAAYAAGLAVGYWKGLDDLKRNWGVDKRWTPKMDAGRRET
IAAAWSRAVQRSFDWKTDED

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=507, Percent_Identity=49.112426035503, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI4504007, Length=507, Percent_Identity=49.112426035503, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI42794763, Length=513, Percent_Identity=48.3430799220273, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI41393575, Length=511, Percent_Identity=47.5538160469667, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI88196792, Length=511, Percent_Identity=32.4853228962818, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI1790361, Length=502, Percent_Identity=51.394422310757, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1790005, Length=477, Percent_Identity=25.1572327044025, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1787789, Length=481, Percent_Identity=27.027027027027, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=43.9363817097416, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71986140, Length=522, Percent_Identity=25.4789272030651, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=29, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=553, Percent_Identity=38.878842676311, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=47.3477406679764, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24654573, Length=435, Percent_Identity=49.4252873563218, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24655555, Length=505, Percent_Identity=43.3663366336634, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655560, Length=505, Percent_Identity=43.3663366336634, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655570, Length=505, Percent_Identity=43.3663366336634, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655574, Length=505, Percent_Identity=43.3663366336634, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655565, Length=505, Percent_Identity=43.3663366336634, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI19922058, Length=538, Percent_Identity=35.6877323420074, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652871, Length=498, Percent_Identity=36.7469879518072, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24656367, Length=517, Percent_Identity=29.5938104448743, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI21357867, Length=527, Percent_Identity=29.0322580645161, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24656372, Length=351, Percent_Identity=31.0541310541311, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=26.8965517241379, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_METRJ (B1LWN6)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754856.1
- ProteinModelPortal:   B1LWN6
- SMR:   B1LWN6
- GeneID:   6138210
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_2178
- HOGENOM:   HBG511469
- OMA:   ELVTSHQ
- ProtClustDB:   CLSK2331969
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54597; Mature: 54466

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 244-244 BINDING 266-266 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYVGAIDQGTTSSRFIVFDREGSVIALAQAEHAQIYPAPGHVEHDATEIWTKTQSVMR
CCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHH
EALEKGGLQPSDLAAVGITNQRETTLIWDRKTGKPLHNALVWQDTRNDRLVAEFARDGGR
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHCEEEECCCCCHHHHHHHHCCCH
DRFRDLTGLPLASYFSGLKLRWLLDHVAGARAKAEAGDVLFGNIDTWLVWNLTGGTEGGL
HHHHHCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEECCCCCCCE
HVTDVTNASRTQLMSLKTLEWDEGMLRTFGIPKAMLPKIVSSSEVYGETRAPFAGVPIAG
EEEECCCCCHHHHHEEEEECCCCCHHHHCCCCHHHHHHHHCCCHHCCCCCCCCCCCCCCE
ILGDQQAALFGQTCFAPGEAKNTYGTGCFALMNTGEEPVPSKAGLVTTLAYRLDGQKPAY
ECCCCHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHEEEEHCCCCCCCE
ALEGSIAITGALVQWLRDNLHMIKDSAEVETLATTVEDNGGVYFVPAFSGLYAPHWNEGA
EECCCEEHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCEEEEECCCCCCCCCCCCCC
RGLIIGLTRYVNRGHIARSVLEATAFQTHEVLDAMAKDSGIPVKELRADGGMVANNTLMQ
CEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCHHHE
FQADMLDVPVVRPKVAETTALGAAYAAGLAVGYWKGLDDLKRNWGVDKRWTPKMDAGRRE
EEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHH
TIAAAWSRAVQRSFDWKTDED
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRYVGAIDQGTTSSRFIVFDREGSVIALAQAEHAQIYPAPGHVEHDATEIWTKTQSVMR
CCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHH
EALEKGGLQPSDLAAVGITNQRETTLIWDRKTGKPLHNALVWQDTRNDRLVAEFARDGGR
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHCEEEECCCCCHHHHHHHHCCCH
DRFRDLTGLPLASYFSGLKLRWLLDHVAGARAKAEAGDVLFGNIDTWLVWNLTGGTEGGL
HHHHHCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEECCCCCCCE
HVTDVTNASRTQLMSLKTLEWDEGMLRTFGIPKAMLPKIVSSSEVYGETRAPFAGVPIAG
EEEECCCCCHHHHHEEEEECCCCCHHHHCCCCHHHHHHHHCCCHHCCCCCCCCCCCCCCE
ILGDQQAALFGQTCFAPGEAKNTYGTGCFALMNTGEEPVPSKAGLVTTLAYRLDGQKPAY
ECCCCHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHEEEEHCCCCCCCE
ALEGSIAITGALVQWLRDNLHMIKDSAEVETLATTVEDNGGVYFVPAFSGLYAPHWNEGA
EECCCEEHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCEEEEECCCCCCCCCCCCCC
RGLIIGLTRYVNRGHIARSVLEATAFQTHEVLDAMAKDSGIPVKELRADGGMVANNTLMQ
CEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCHHHE
FQADMLDVPVVRPKVAETTALGAAYAAGLAVGYWKGLDDLKRNWGVDKRWTPKMDAGRRE
EEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHH
TIAAAWSRAVQRSFDWKTDED
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA