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The map label for this gene is queA

Identifier: 170746470

GI number: 170746470

Start: 23543

End: 24607

Strand: Reverse

Name: queA

Synonym: Mrad2831_0020

Alternate gene names: 170746470

Gene position: 24607-23543 (Counterclockwise)

Preceding gene: 170746474

Following gene: 170746469

Centisome position: 0.4

GC content: 73.62

Gene sequence:

>1065_bases
ATGCGCGTGGACCTCTTCGATTTCGAGTTGCCGGAGACCAGCATCGCCCTGCGCCCGGCCGAGCCGCGCGACGCCGGGCG
GATGCTCGTGGTCCGCCCCGGTGCGCCGCTGGTCGACCGGACCGTGCGCGACCTGCCCGACGCTCTCCGGGCCGGCGACG
CCCTGGTCTTCAACGACACGCGGGTGATCCCCGCCCGGTTGAACGGCGTGCGCACCCGCCCCGGCGCCCCCGGCCAGCGC
ACGGAGGTGATGCTCCACCTGCGCGAGGCCCCCGACCGGTGGCGCGCCTTCGCGCGTCCGGCCAAGCGGCTCACGGCGGG
CGACGCGCTGCGCTTCGGCGATCTGACGGCGACCGTTCTCGAGAAGGCCGAGGCCGGCGAGATCGTCCTGGCCTTCGACC
GGGCCGGGCCCGAGCTCGACGCGGCCATCGCCGCGGAGGGCGCCCTGCCGCTGCCCCCCTACATCGCGGGCAAGCGCGCC
ACGGATGCCCGCGACGCCACTGATTACCAGACGGTCTACGCCCGCAATCCCGGGGCGGTGGCGGCCCCGACGGCCGGCCT
GCACTTCTCGGACGCCCTGCTGGCCGATCTCGACGCCGCCGGTCTGCAGCGCCATCACGTCACGTTGCATGTCGGCGCCG
GGACCTTTCTGCCGGTCAAGGCCGAGGACACCGACGGGCACCGCATGCACGCGGAGATCGGCATCCTCGACGCCGCCACG
GCGGACGCCCTGAATGCCGCGCGGGCCGCCGGCGGCCGGATCGTCGCGGTCGGAACCACGGCTCTGCGCCTGCTGGAGAG
CGCGGCCCGCCCGGACGGGACACTCGCACCCTTTTCCGGACCGACCGAGATCTTCATCACCCCGGGCTACCGGTTCCGGG
CGGTCGACGCTCTCGTGACCAACTTCCACCTGCCACGCTCGACGCTGTTCATGCTGGTCTCAGCCTTCTCGGGCCTCGAG
ACGATGCGCGCGGCCTACGCGCACGCGATCGGCGCCGGGTACCGGTTCTACTCCTACGGCGACGCCAGCCTGCTCTTCCC
GGGCCCGCGCGCGGACACTCCATGA

Upstream 100 bases:

>100_bases
ATGGGTCGGGTGCCTCGCGTCTCGGTGACGCGGAAAACGGACGCGCTCCTCCGCGGTTCCGCGGTTGAAACCGCGTCCCG
TTCGGTGCAGGCAGCCGGCG

Downstream 100 bases:

>100_bases
CCGACAGCGCTCCCTTCCGCTTCGACCTGCTGGCCCGGGACGGCACCGCCCGGACCGGCATGATCGCGACGCCGCGCGGC
GAGATCCGGACCCCGGCCTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRVDLFDFELPETSIALRPAEPRDAGRMLVVRPGAPLVDRTVRDLPDALRAGDALVFNDTRVIPARLNGVRTRPGAPGQR
TEVMLHLREAPDRWRAFARPAKRLTAGDALRFGDLTATVLEKAEAGEIVLAFDRAGPELDAAIAAEGALPLPPYIAGKRA
TDARDATDYQTVYARNPGAVAAPTAGLHFSDALLADLDAAGLQRHHVTLHVGAGTFLPVKAEDTDGHRMHAEIGILDAAT
ADALNAARAAGGRIVAVGTTALRLLESAARPDGTLAPFSGPTEIFITPGYRFRAVDALVTNFHLPRSTLFMLVSAFSGLE
TMRAAYAHAIGAGYRFYSYGDASLLFPGPRADTP

Sequences:

>Translated_354_residues
MRVDLFDFELPETSIALRPAEPRDAGRMLVVRPGAPLVDRTVRDLPDALRAGDALVFNDTRVIPARLNGVRTRPGAPGQR
TEVMLHLREAPDRWRAFARPAKRLTAGDALRFGDLTATVLEKAEAGEIVLAFDRAGPELDAAIAAEGALPLPPYIAGKRA
TDARDATDYQTVYARNPGAVAAPTAGLHFSDALLADLDAAGLQRHHVTLHVGAGTFLPVKAEDTDGHRMHAEIGILDAAT
ADALNAARAAGGRIVAVGTTALRLLESAARPDGTLAPFSGPTEIFITPGYRFRAVDALVTNFHLPRSTLFMLVSAFSGLE
TMRAAYAHAIGAGYRFYSYGDASLLFPGPRADTP
>Mature_354_residues
MRVDLFDFELPETSIALRPAEPRDAGRMLVVRPGAPLVDRTVRDLPDALRAGDALVFNDTRVIPARLNGVRTRPGAPGQR
TEVMLHLREAPDRWRAFARPAKRLTAGDALRFGDLTATVLEKAEAGEIVLAFDRAGPELDAAIAAEGALPLPPYIAGKRA
TDARDATDYQTVYARNPGAVAAPTAGLHFSDALLADLDAAGLQRHHVTLHVGAGTFLPVKAEDTDGHRMHAEIGILDAAT
ADALNAARAAGGRIVAVGTTALRLLESAARPDGTLAPFSGPTEIFITPGYRFRAVDALVTNFHLPRSTLFMLVSAFSGLE
TMRAAYAHAIGAGYRFYSYGDASLLFPGPRADTP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=358, Percent_Identity=45.5307262569832, Blast_Score=260, Evalue=9e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_METRJ (B1M584)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001752730.1
- ProteinModelPortal:   B1M584
- SMR:   B1M584
- GeneID:   6136319
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_0020
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37701; Mature: 37701

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFELPETSIALRPAEPRDAGRMLVVRPGAPLVDRTVRDLPDALRAGDALVFNDT
CEEEEEECCCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECC
RVIPARLNGVRTRPGAPGQRTEVMLHLREAPDRWRAFARPAKRLTAGDALRFGDLTATVL
EEEEHHCCCEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCHHHHHH
EKAEAGEIVLAFDRAGPELDAAIAAEGALPLPPYIAGKRATDARDATDYQTVYARNPGAV
HHCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCE
AAPTAGLHFSDALLADLDAAGLQRHHVTLHVGAGTFLPVKAEDTDGHRMHAEIGILDAAT
ECCCCCCCCCHHHHHHCCCCCCEEEEEEEEECCCEEEEEECCCCCCCEEEEEEEEEECCH
ADALNAARAAGGRIVAVGTTALRLLESAARPDGTLAPFSGPTEIFITPGYRFRAVDALVT
HHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHH
NFHLPRSTLFMLVSAFSGLETMRAAYAHAIGAGYRFYSYGDASLLFPGPRADTP
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCCCC
>Mature Secondary Structure
MRVDLFDFELPETSIALRPAEPRDAGRMLVVRPGAPLVDRTVRDLPDALRAGDALVFNDT
CEEEEEECCCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECC
RVIPARLNGVRTRPGAPGQRTEVMLHLREAPDRWRAFARPAKRLTAGDALRFGDLTATVL
EEEEHHCCCEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCHHHHHH
EKAEAGEIVLAFDRAGPELDAAIAAEGALPLPPYIAGKRATDARDATDYQTVYARNPGAV
HHCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCE
AAPTAGLHFSDALLADLDAAGLQRHHVTLHVGAGTFLPVKAEDTDGHRMHAEIGILDAAT
ECCCCCCCCCHHHHHHCCCCCCEEEEEEEEECCCEEEEEECCCCCCCEEEEEEEEEECCH
ADALNAARAAGGRIVAVGTTALRLLESAARPDGTLAPFSGPTEIFITPGYRFRAVDALVT
HHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHH
NFHLPRSTLFMLVSAFSGLETMRAAYAHAIGAGYRFYSYGDASLLFPGPRADTP
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA