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The map label for this gene is tgt [H]

Identifier: 170746469

GI number: 170746469

Start: 22407

End: 23546

Strand: Reverse

Name: tgt [H]

Synonym: Mrad2831_0019

Alternate gene names: 170746469

Gene position: 23546-22407 (Counterclockwise)

Preceding gene: 170746470

Following gene: 170746467

Centisome position: 0.39

GC content: 71.58

Gene sequence:

>1140_bases
ATGACCGACAGCGCTCCCTTCCGCTTCGACCTGCTGGCCCGGGACGGCACCGCCCGGACCGGCATGATCGCGACGCCGCG
CGGCGAGATCCGGACCCCGGCCTTCATGCCGGTGGGCACCGCCGCGACGGTCAAGGCGATGTATCCCGGCCAGGTCCGTG
ACCTCGGCGCCGATGTGGTGCTGGGCAACACCTATCACCTGATGCTGCGGCCCGGCCCGGAGCGCATGGCCCGGCTCGGC
GGCCTGCACCGCTTCATGAACTGGGACCGGCCGATCCTGACGGATTCCGGCGGCTTTCAAGTGATGTCGCTCTCCGCCCT
GCGGAAGATCGACGAGCAGGGCGTGACCTTCCGCTCACACATCGACGGCACAGCGCACCACATGAGCCCGGAGCGCTCCA
TCGAGATTCAGGGTCTGCTCGGCTCCGACATCCAGATGCAGCTCGACGAGTGCGTCCGCCTGCCGGCGGATCACGCGGCC
ATCGAGAAGGCCATGCACCTGTCCCTGCGCTGGGCCGAGCGCTGCCGCGTCGCGTTCGGCGAGCAGCCCGGGCGAGCCCT
GTTCGGCATCGTCCAGGGCGGGGACGTGCCTGCCCTGCGCGTCGAGAGCGCCCGCGCGCTGACCGATCTCGATCTCAAGG
GCTACGCGGTGGGCGGCCTCGCCGTGGGCGAGCCCCAGGCCGTCATGTTCGCCATGATCGAGACGGTGGAGCCGCACCTG
CCGGCCGCGAAGCCCCGCTACCTGATGGGCGTCGGCACGCCGGACGACATCCTCGGCGCGGTGGGGCGCGGCATCGACAT
GTTCGACTGCGTGATGCCGACCCGCGCCGGCCGGCACGGCCTCGCCTACACGCGCCACGGGCGGGTCAACCTGCGCAACG
CCCGCCACGCGGAAGACACCGCGCCGCTCGACGTGGCCTCGACCTGTCCGGCCGCGCGGGACTATTCCCGGGCCTACCTG
CACCACCTCGTGCGCTCGAACGAGATCCTCGGGATGATGCTGCTGACGTGGAACAACCTCGCCTACTACCAGGACCTGAT
GGCCGGCGCCCGCGCGGCGATCCGCGACGGCCGCTACGCGGATTACTGCGCCGAGACCCGCGCGGGCTGGGCCAGCGCCG
AGCGCGCCGCCGCCGCTTGA

Upstream 100 bases:

>100_bases
CGATGCGCGCGGCCTACGCGCACGCGATCGGCGCCGGGTACCGGTTCTACTCCTACGGCGACGCCAGCCTGCTCTTCCCG
GGCCCGCGCGCGGACACTCC

Downstream 100 bases:

>100_bases
CCGGGCATCACGGGTGGGCGAGCACCCGGTAGACCCCATCCGGATCGTCGCGGCCGGTGGCGACGGCGCTCGCGAGATCC
GAGACGATGCGCGCCGCCAC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTDSAPFRFDLLARDGTARTGMIATPRGEIRTPAFMPVGTAATVKAMYPGQVRDLGADVVLGNTYHLMLRPGPERMARLG
GLHRFMNWDRPILTDSGGFQVMSLSALRKIDEQGVTFRSHIDGTAHHMSPERSIEIQGLLGSDIQMQLDECVRLPADHAA
IEKAMHLSLRWAERCRVAFGEQPGRALFGIVQGGDVPALRVESARALTDLDLKGYAVGGLAVGEPQAVMFAMIETVEPHL
PAAKPRYLMGVGTPDDILGAVGRGIDMFDCVMPTRAGRHGLAYTRHGRVNLRNARHAEDTAPLDVASTCPAARDYSRAYL
HHLVRSNEILGMMLLTWNNLAYYQDLMAGARAAIRDGRYADYCAETRAGWASAERAAAA

Sequences:

>Translated_379_residues
MTDSAPFRFDLLARDGTARTGMIATPRGEIRTPAFMPVGTAATVKAMYPGQVRDLGADVVLGNTYHLMLRPGPERMARLG
GLHRFMNWDRPILTDSGGFQVMSLSALRKIDEQGVTFRSHIDGTAHHMSPERSIEIQGLLGSDIQMQLDECVRLPADHAA
IEKAMHLSLRWAERCRVAFGEQPGRALFGIVQGGDVPALRVESARALTDLDLKGYAVGGLAVGEPQAVMFAMIETVEPHL
PAAKPRYLMGVGTPDDILGAVGRGIDMFDCVMPTRAGRHGLAYTRHGRVNLRNARHAEDTAPLDVASTCPAARDYSRAYL
HHLVRSNEILGMMLLTWNNLAYYQDLMAGARAAIRDGRYADYCAETRAGWASAERAAAA
>Mature_378_residues
TDSAPFRFDLLARDGTARTGMIATPRGEIRTPAFMPVGTAATVKAMYPGQVRDLGADVVLGNTYHLMLRPGPERMARLGG
LHRFMNWDRPILTDSGGFQVMSLSALRKIDEQGVTFRSHIDGTAHHMSPERSIEIQGLLGSDIQMQLDECVRLPADHAAI
EKAMHLSLRWAERCRVAFGEQPGRALFGIVQGGDVPALRVESARALTDLDLKGYAVGGLAVGEPQAVMFAMIETVEPHLP
AAKPRYLMGVGTPDDILGAVGRGIDMFDCVMPTRAGRHGLAYTRHGRVNLRNARHAEDTAPLDVASTCPAARDYSRAYLH
HLVRSNEILGMMLLTWNNLAYYQDLMAGARAAIRDGRYADYCAETRAGWASAERAAAA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=362, Percent_Identity=43.9226519337017, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1786607, Length=359, Percent_Identity=54.0389972144847, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17544680, Length=359, Percent_Identity=42.3398328690808, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI19920510, Length=362, Percent_Identity=44.7513812154696, Blast_Score=313, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41336; Mature: 41205

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSAPFRFDLLARDGTARTGMIATPRGEIRTPAFMPVGTAATVKAMYPGQVRDLGADVV
CCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCEE
LGNTYHLMLRPGPERMARLGGLHRFMNWDRPILTDSGGFQVMSLSALRKIDEQGVTFRSH
ECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHCCCEEEHH
IDGTAHHMSPERSIEIQGLLGSDIQMQLDECVRLPADHAAIEKAMHLSLRWAERCRVAFG
CCCHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
EQPGRALFGIVQGGDVPALRVESARALTDLDLKGYAVGGLAVGEPQAVMFAMIETVEPHL
CCCCCEEEEEEECCCCCEEEECCCHHHCCCCCCCEEECCEECCCCHHHHHHHHHHHCCCC
PAAKPRYLMGVGTPDDILGAVGRGIDMFDCVMPTRAGRHGLAYTRHGRVNLRNARHAEDT
CCCCCCEEEECCCCHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCCCCC
APLDVASTCPAARDYSRAYLHHLVRSNEILGMMLLTWNNLAYYQDLMAGARAAIRDGRYA
CCCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCH
DYCAETRAGWASAERAAAA
HHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
TDSAPFRFDLLARDGTARTGMIATPRGEIRTPAFMPVGTAATVKAMYPGQVRDLGADVV
CCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCEE
LGNTYHLMLRPGPERMARLGGLHRFMNWDRPILTDSGGFQVMSLSALRKIDEQGVTFRSH
ECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHCCCEEEHH
IDGTAHHMSPERSIEIQGLLGSDIQMQLDECVRLPADHAAIEKAMHLSLRWAERCRVAFG
CCCHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
EQPGRALFGIVQGGDVPALRVESARALTDLDLKGYAVGGLAVGEPQAVMFAMIETVEPHL
CCCCCEEEEEEECCCCCEEEECCCHHHCCCCCCCEEECCEECCCCHHHHHHHHHHHCCCC
PAAKPRYLMGVGTPDDILGAVGRGIDMFDCVMPTRAGRHGLAYTRHGRVNLRNARHAEDT
CCCCCCEEEECCCCHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCCCCC
APLDVASTCPAARDYSRAYLHHLVRSNEILGMMLLTWNNLAYYQDLMAGARAAIRDGRYA
CCCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCH
DYCAETRAGWASAERAAAA
HHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]