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The map label for this gene is bisC [H]

Identifier: 170681133

GI number: 170681133

Start: 3940209

End: 3942542

Strand: Reverse

Name: bisC [H]

Synonym: EcSMS35_3871

Alternate gene names: 170681133

Gene position: 3942542-3940209 (Counterclockwise)

Preceding gene: 170680625

Following gene: 170681569

Centisome position: 77.79

GC content: 54.76

Gene sequence:

>2334_bases
TTGGCCAACTCATCCTCACGATATTCCGTTCTGACTGCCGCCCACTGGGGGCCTATGCTGGTTGAAACCGACGGCGAAAC
CGTGTTTAGCTCGCGTGGCGCGTTAGCCACAGGAATGGAAAACTCCTTGCAGAGCGCGGTTCGCGACCAGGTTCACAGCA
ATACGCGGGTGCGATTTCCAATGGTGCGCAAAGGCTTTCTTGCGTCACCGGAAAATCCGCAAGGCATTCGTGGGCAGGAT
GAATTTGTTCGCGTGAGTTGGGATGAGGCGCTGGAGCTTATTCACCATCAACATAAACGCATTCGTGAGGCTTATGGTCC
GGCATCGATTTTTGCGGGTTCCTACGGCTGGCGTTCAAACGGCGTGCTGCATAAGGCCTCGACATTATTACAACGCTATA
TGGCGCTGGCAGGCGGTTATACTGGGCATCTGGGGGATTATTCGACCGGCGCGGCACAGGCGATCATGCCGTATGTCGTG
GGGGGCAGCGAAGTTTATCAACAGCAAACCAGTTGGCCGCTGGTGCTGGAACATAGCGATGTCGTGGTGCTGTGGAGTGC
TAACCCACTCAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCCGCACTGCGTGACA
GCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGATTTCTTTGGCGATAAAATGGAATGGGTGGCA
CCGCACATGGGCACCGATGTTGCGCTGATGCTGGGGATCGCCTATACGCTGGTGGAAAATGGTTGGCACGACGAAGCGTT
TCTGGCGCGTTGCACCACAGGTTATGCCGTCTTCGCCTCTTATTTGCTGGGCGAGAGTGACGGAATAGCGAAAAACGCCG
AATGGGCGGCAGAGATTTGTGGTGTTGGCGCAGCGAAAATCCGCGAGCTGGCGGCTCTTTTCCACCAAAATACCACCATG
CTGATGGCTGGCTGGGGAATGCAACGTCAACAGTTTGGCGAGCAAAAGCACTGGATGATCGTCACGCTGGCGGCAATGTT
GGGGCAAATCGGCACACCCGGCGGCGGTTTTGGTCTTTCTTACCATTTTGCCAATGGTGGTAACCCCACGCGCCGCGCTG
CGGTGCTCTCTTCCATGCAGGGTAGCTTGCCTGGCGGCACCGATGCGGTGGATAAAATCCCTGTTGCCCGCATTGTTGAA
GCACTGGAAAACCCCGGTGGCGCATATCAACATAACGGTATGGACCGACATTTCCCGGATATTCGTTTTATCTGGTGGGC
GGGCGGTGCCAACTTTACTCATCATCAGGATACCAATCGCCTGATCCGTGCCTGGCAAAAACCGGAGCTGGTGGTGATCT
CTGAATGCTTCTGGACGGCTGCGGCGAAACACGCGGATATCGTTCTGCCTGCGACCACTTCGTTTGAGCGTAATGATCTC
ACCATGACCGGCGATTACAGTAATCAGCATCTGGTGCCGATGAAGCAAGTGGTGCCTCCACGTTATGAAGCGCGTAATGA
TTTTGATGTCTTTGCCGAGTTGAGTGAACGTTGGGAGAAGGGCGGCTATGCGCGGTTTACGGAAGGAAAAAGTGAGCTGC
AATGGCTGGAAACGTTTTATAACGTTGCCCGGCAGCGCGGGGCAAGCCAGCAGGTTGAATTGCCGCCATTTGCTGAGTTC
TGGGAAGCCAACCAGTTAATTGAGATGCCGGAAAACCCGGACAGCGAGCGGTTTATTCGCTTCGCCGATTTTCGCCGCGA
TCCGCAGGCGCATCCATTAAAAACCGCCAGCGGTAAGATTGAAATCTTCTCGCAGCGTATTGCCGATTACGCTTATCCGG
ATTGCCCTGGGCATCCAATGTGGCTGGAGCCGGACGAATGGCAGGGCAATGCCGAACCGGAACAGTTGCAGGTACTTTCT
GCTCATCCGGCACATCGTCTGCACAGCCAGCTGAATTACAGTTCTCTGCGCGAATTGTACGCGGTGGCAAATCGTGAGCC
TGTCACCATTCATCCTGACGATGCCCAGGCGCGCGGCATACAAGATGGCGATATTGTTCGGTTGTGGAACGCACGCGGGC
AAATTCTTGCCGGAGCGGTCATTAGCGAGGGAATTAAACCTGGCGTGATTTGCATTCATGAAGGGGCATGGCCGGATCTG
GATTTAACCGCTGACGGTATTTGTAAAAACGGCGCGGTGAACGTTCTGACCAAAGATCTCCGCAGCTCGCGGCTGGGGAA
TGGCTGTGCGGGTAATACGGCGCTGGCATGGCTGGAAAAATACAACGGTCCGGAACTGACACTTACAGCGTTTGAACCAC
CGGCCAGCTCATAA

Upstream 100 bases:

>100_bases
ATTATGCCGCGTGAACATGAAGATTATTCCTGGGAATACTCGGAAATTTGTAAGTAATATTTAACTGCTCAATACATCTA
ACCTTTCAGGAGTCTTCGGT

Downstream 100 bases:

>100_bases
TCCAGGTGGGTAGTTGGGTTTCATCTTGCCATGCGCAGTCGACAATGTGAAAACCCTGCGCATGGTAAAAATCTATCGCC
GGTTGATTTTTTTGATAAAC

Product: biotin sulfoxide reductase

Products: Biotin. [C]

Alternate protein names: BDS reductase; BSO reductase [H]

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQD
EFVRVSWDEALELIHHQHKRIREAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVA
PHMGTDVALMLGIAYTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAALFHQNTTM
LMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDKIPVARIVE
ALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDL
TMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEF
WEANQLIEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLS
AHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQDGDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDL
DLTADGICKNGAVNVLTKDLRSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS

Sequences:

>Translated_777_residues
MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQD
EFVRVSWDEALELIHHQHKRIREAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVA
PHMGTDVALMLGIAYTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAALFHQNTTM
LMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDKIPVARIVE
ALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDL
TMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEF
WEANQLIEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLS
AHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQDGDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDL
DLTADGICKNGAVNVLTKDLRSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS
>Mature_776_residues
ANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDE
FVRVSWDEALELIHHQHKRIREAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVG
GSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAP
HMGTDVALMLGIAYTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAALFHQNTTML
MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDKIPVARIVEA
LENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLT
MTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFW
EANQLIEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLSA
HPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQDGDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLD
LTADGICKNGAVNVLTKDLRSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS

Specific function: This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source. It reduces a spontaneous oxidation product of biotin, D-biotin D-sulfoxide (BSO or BDS), back to biotin [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI145693196, Length=777, Percent_Identity=98.0694980694981, Blast_Score=1587, Evalue=0.0,
Organism=Escherichia coli, GI87081994, Length=773, Percent_Identity=63.3893919793014, Blast_Score=1056, Evalue=0.0,
Organism=Escherichia coli, GI1787231, Length=790, Percent_Identity=42.7848101265823, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI87081797, Length=757, Percent_Identity=32.4966974900925, Blast_Score=310, Evalue=3e-85,
Organism=Escherichia coli, GI1787870, Length=753, Percent_Identity=30.6772908366534, Blast_Score=298, Evalue=1e-81,
Organism=Escherichia coli, GI171474008, Length=753, Percent_Identity=30.4116865869854, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI3868721, Length=474, Percent_Identity=23.4177215189873, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006658
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006655 [H]

Pfam domain/function: PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 85993; Mature: 85861

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2 ; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFP
CCCCCCCEEEEEEECCCCEEEECCCCCEECCCCCCHHCHHHHHHHHHHHHHHCCCEEEEH
MVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIREAYGPASIFAGSYGWRSN
HHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCC
GVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSD
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHCCCCCEEEECCC
VVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVA
EEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCHHHHC
PHMGTDVALMLGIAYTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEIC
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GVGAAKIRELAALFHQNTTMLMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLS
CCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEE
YHFANGGNPTRRAAVLSSMQGSLPGGTDAVDKIPVARIVEALENPGGAYQHNGMDRHFPD
EEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCC
IRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDL
EEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCC
TMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFY
EEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
NVARQRGASQQVELPPFAEFWEANQLIEMPENPDSERFIRFADFRRDPQAHPLKTASGKI
HHHHHCCCCCCCCCCCHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCH
EIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELY
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCHHHHHHHCCHHHHHHHH
AVANREPVTIHPDDAQARGIQDGDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDL
HHCCCCCEEECCCCHHHCCCCCCCEEEEECCCCCEEEHHHHHCCCCCCEEEEECCCCCCC
DLTADGICKNGAVNVLTKDLRSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
ANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFP
CCCCCCEEEEEEECCCCEEEECCCCCEECCCCCCHHCHHHHHHHHHHHHHHCCCEEEEH
MVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIREAYGPASIFAGSYGWRSN
HHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCC
GVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSD
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHCCCCCEEEECCC
VVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVA
EEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCHHHHC
PHMGTDVALMLGIAYTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEIC
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GVGAAKIRELAALFHQNTTMLMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLS
CCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEE
YHFANGGNPTRRAAVLSSMQGSLPGGTDAVDKIPVARIVEALENPGGAYQHNGMDRHFPD
EEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCC
IRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDL
EEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCC
TMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFY
EEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
NVARQRGASQQVELPPFAEFWEANQLIEMPENPDSERFIRFADFRRDPQAHPLKTASGKI
HHHHHCCCCCCCCCCCHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCH
EIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELY
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCHHHHHHHCCHHHHHHHH
AVANREPVTIHPDDAQARGIQDGDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDL
HHCCCCCEEECCCCHHHCCCCCCCEEEEECCCCCEEEHHHHHCCCCCCEEEEECCCCCCC
DLTADGICKNGAVNVLTKDLRSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Spontaneous Oxidation Product Of Biotin, D-Biotin D-Sulfoxide (Bso Or Bds), [C]

Specific reaction: Reduces A Spontaneous Oxidation Product Of Biotin, D-Biotin D-Sulfoxide (Bso Or Bds), Back To Biotin. [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180922; 8041620; 9278503 [H]