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The map label for this gene is fanE [H]

Identifier: 170679645

GI number: 170679645

Start: 3999816

End: 4000496

Strand: Reverse

Name: fanE [H]

Synonym: EcSMS35_3925

Alternate gene names: 170679645

Gene position: 4000496-3999816 (Counterclockwise)

Preceding gene: 170681449

Following gene: 170682510

Centisome position: 78.93

GC content: 39.06

Gene sequence:

>681_bases
ATGAAAAAAATAAATCTCATGCCAGTATTGGCTTTGCTGCTGATCTGCTTTAATAGCCATGCTGCCTTTGTTCTCAATAG
TACGCGTTATATTTTTGCGGGCGATAAGGATAACATTTCAGTCCAGGTCAATAATGAATCTTCTCAAGAGTATGGCGGTC
AAATCTGGATCGATAATGCAAATGCAAGAGATCAGAACGTTTACTTTGCCCCCAGTCCGACCTTTTTCAAAGTTTCTGGT
GGACATAAACAAATTTTGCGTCTCCTGAAAATTAATGACAGTTTACCCAAAGACAAAGAATCACTGTTTTGGGTCAACGT
CCAGGAAATCCCTAAAGCACCAAAAGAGGGCACAAACTCCCTTGCCATAGCATTGCATACTCAGGTTAAGATGATTTATC
GGCCTGAGTCACTAAAAGAGGGTAGGGAAGATGCGGAGAAAAATATCAAGATCATCCATGAGGGGGGAAATACGATTCTA
TATAATAACTCTCCGTACTATTTTGCAGTGATTAATGTAAAGCAAAATGGTCATGATGTGAAACTTAGCGAAGAAACTAA
AAATAAGATCGCGGCTTTTGCGCCTTTTGAGAAGGTATCTCTGGGAAGTAAACTTTCAGGAAAAGATATTTCAATTGTTT
CTTTTGATGATTATGGCGTAGACCGGGAATATAAACTCTAG

Upstream 100 bases:

>100_bases
GATGAAGGTGTTATTTCGCTTGACCAGTGTCAATTTAATACGCAGTCACTGAAGAAAAACTATGACCAAACTCAGGAGAT
TCACTGTGAGTAACAATGTA

Downstream 100 bases:

>100_bases
CCCAAACCTTTATTATTAAGGAGAATGCTATGCAGAAATTATTATTTCTTGTATTTACGCTATTTTTTATTTCGCCACAT
AGTCATGCGGCGGTGGATTG

Product: putative fimbrial assembly chaperone FanE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKKINLMPVLALLLICFNSHAAFVLNSTRYIFAGDKDNISVQVNNESSQEYGGQIWIDNANARDQNVYFAPSPTFFKVSG
GHKQILRLLKINDSLPKDKESLFWVNVQEIPKAPKEGTNSLAIALHTQVKMIYRPESLKEGREDAEKNIKIIHEGGNTIL
YNNSPYYFAVINVKQNGHDVKLSEETKNKIAAFAPFEKVSLGSKLSGKDISIVSFDDYGVDREYKL

Sequences:

>Translated_226_residues
MKKINLMPVLALLLICFNSHAAFVLNSTRYIFAGDKDNISVQVNNESSQEYGGQIWIDNANARDQNVYFAPSPTFFKVSG
GHKQILRLLKINDSLPKDKESLFWVNVQEIPKAPKEGTNSLAIALHTQVKMIYRPESLKEGREDAEKNIKIIHEGGNTIL
YNNSPYYFAVINVKQNGHDVKLSEETKNKIAAFAPFEKVSLGSKLSGKDISIVSFDDYGVDREYKL
>Mature_226_residues
MKKINLMPVLALLLICFNSHAAFVLNSTRYIFAGDKDNISVQVNNESSQEYGGQIWIDNANARDQNVYFAPSPTFFKVSG
GHKQILRLLKINDSLPKDKESLFWVNVQEIPKAPKEGTNSLAIALHTQVKMIYRPESLKEGREDAEKNIKIIHEGGNTIL
YNNSPYYFAVINVKQNGHDVKLSEETKNKIAAFAPFEKVSLGSKLSGKDISIVSFDDYGVDREYKL

Specific function: Mediates assembly of pili by forming soluble multimeric complexes with pili subunits as an intermediate step in the assembly process. This protein is involved in K99 pili assembly [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1789532, Length=227, Percent_Identity=30.3964757709251, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1790771, Length=211, Percent_Identity=28.436018957346, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1786743, Length=226, Percent_Identity=28.7610619469027, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786936, Length=224, Percent_Identity=26.7857142857143, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788428, Length=228, Percent_Identity=26.7543859649123, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787171, Length=232, Percent_Identity=27.5862068965517, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1786333, Length=195, Percent_Identity=29.7435897435897, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788677, Length=205, Percent_Identity=25.8536585365854, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87081806, Length=177, Percent_Identity=25.9887005649718, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082203, Length=231, Percent_Identity=24.6753246753247, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25442; Mature: 25442

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKINLMPVLALLLICFNSHAAFVLNSTRYIFAGDKDNISVQVNNESSQEYGGQIWIDNA
CCCCCHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCEEEEECCCCCHHCCCEEEEECC
NARDQNVYFAPSPTFFKVSGGHKQILRLLKINDSLPKDKESLFWVNVQEIPKAPKEGTNS
CCCCCEEEECCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCCCCCCCCE
LAIALHTQVKMIYRPESLKEGREDAEKNIKIIHEGGNTILYNNSPYYFAVINVKQNGHDV
EEEEEEEEEEEEECCHHHHHHHHHHCCCEEEEEECCCEEEECCCCEEEEEEEEECCCCEE
KLSEETKNKIAAFAPFEKVSLGSKLSGKDISIVSFDDYGVDREYKL
EECCHHCCCEEEECCHHHHCCCCCCCCCEEEEEEECCCCCCCEECC
>Mature Secondary Structure
MKKINLMPVLALLLICFNSHAAFVLNSTRYIFAGDKDNISVQVNNESSQEYGGQIWIDNA
CCCCCHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCEEEEECCCCCHHCCCEEEEECC
NARDQNVYFAPSPTFFKVSGGHKQILRLLKINDSLPKDKESLFWVNVQEIPKAPKEGTNS
CCCCCEEEECCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCCCCCCCCE
LAIALHTQVKMIYRPESLKEGREDAEKNIKIIHEGGNTILYNNSPYYFAVINVKQNGHDV
EEEEEEEEEEEEECCHHHHHHHHHHCCCEEEEEECCCEEEECCCCEEEEEEEEECCCCEE
KLSEETKNKIAAFAPFEKVSLGSKLSGKDISIVSFDDYGVDREYKL
EECCHHCCCEEEECCHHHHCCCCCCCCCEEEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1713284; 7908067; 1982454 [H]