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The map label for this gene is trmH [H]

Identifier: 170026386

GI number: 170026386

Start: 4607836

End: 4608495

Strand: Direct

Name: trmH [H]

Synonym: YPK_4179

Alternate gene names: 170026386

Gene position: 4607836-4608495 (Clockwise)

Preceding gene: 170026385

Following gene: 170026387

Centisome position: 98.26

GC content: 49.7

Gene sequence:

>660_bases
ATGCTTGCCACCAGGCAACCCGATTTAACGGTCTGTCTGGAGCAAGTCCACAAACCTCATAATGTCTCCGCCATTATTCG
AACTGCCGATGCCGTGGGCATCCATCAAGTTCATGCCATCTGGCCAACAACGCAAATGTATACTCGGCTGTCCGCCGCCG
CAGGCAGTAATAGCTGGGTGCAAGTCAAAACGCATCCCCATATTGCTGATGCCATTGCTTATCTAAAATCACAAGATATG
CAAATTCTGGCGACACATCTGTCTGATAAGGCTGTTGATTTTCGTGAAATCGATTATACCCGCCCTACCTGTATTTTAAT
GGGTCAGGAAAAAACCGGTATTTCACCAGAAGCGCTGGCACTGGCCGATCAAGACATCATCATTCCAATGATCGGCATGG
TACAGTCGCTGAATGTTTCCGTCGCTTCCGCGCTAATTTTATATGAAGCACAGCGGCAACGGCAGAATGCCGGGATGTAT
AAACGGACACACAGCGTTCTGGCAGAGAGTGAGCAGCAACGGCTATTGTTTGAAGGGGGTTACCCGGTATTGGCACACGT
TGCCAAACGTAAAGGGCTCCCCCAACCTCATATTGATGAACAAGGCCAAATCATTGCCGATGCGCAATGGTGGTCGGCCA
TGCAAGCCACGGAGTCTTAA

Upstream 100 bases:

>100_bases
ACATTATGCGTAAAATCCGTATCATGCCAGATGTGGTTAAAGTTAGCCGTAATCGCAATTAACGCCTATGAATCCTCAAC
GCTATGCGCGGATTTGTGAC

Downstream 100 bases:

>100_bases
ATGAAAGGCCGCCTACTGGACGCCGTACCCCTCAGTACACTTTCCGGAGTTGGCGCAAGTCAGGCTGGAAAACTCGCCAA
GATAGGTTTGGAAACCATTC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTRLSAAAGSNSWVQVKTHPHIADAIAYLKSQDM
QILATHLSDKAVDFREIDYTRPTCILMGQEKTGISPEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMY
KRTHSVLAESEQQRLLFEGGYPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES

Sequences:

>Translated_219_residues
MLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTRLSAAAGSNSWVQVKTHPHIADAIAYLKSQDM
QILATHLSDKAVDFREIDYTRPTCILMGQEKTGISPEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMY
KRTHSVLAESEQQRLLFEGGYPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES
>Mature_219_residues
MLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTRLSAAAGSNSWVQVKTHPHIADAIAYLKSQDM
QILATHLSDKAVDFREIDYTRPTCILMGQEKTGISPEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMY
KRTHSVLAESEQQRLLFEGGYPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=216, Percent_Identity=82.8703703703704, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1790623, Length=153, Percent_Identity=33.9869281045752, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 24283; Mature: 24283

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTRLSAAAGSNSWV
CCCCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCEE
QVKTHPHIADAIAYLKSQDMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGISPEALA
EEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCCHHEE
LADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYKRTHSVLAESEQQRLLFEGG
ECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHEEEEECC
YPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES
CHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHCCC
>Mature Secondary Structure
MLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTRLSAAAGSNSWV
CCCCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCEE
QVKTHPHIADAIAYLKSQDMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGISPEALA
EEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCCHHEE
LADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYKRTHSVLAESEQQRLLFEGG
ECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHEEEEECC
YPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES
CHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]