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The map label for this gene is spoT [H]

Identifier: 170026385

GI number: 170026385

Start: 4605689

End: 4607797

Strand: Direct

Name: spoT [H]

Synonym: YPK_4178

Alternate gene names: 170026385

Gene position: 4605689-4607797 (Clockwise)

Preceding gene: 170026384

Following gene: 170026386

Centisome position: 98.21

GC content: 48.98

Gene sequence:

>2109_bases
TTGTACCTGTTTGAAAGCCTGAATCTGCTGATTCAACGTTACCTGCCAGAGGAGCAGATTAAGCGCCTCAAACAGGCATA
TCTTGTCGCACGTGATGCTCACGAGGGGCAGACACGCTCCAGCGGTGAGCCTTATATCACTCACCCGGTTGCTGTGGCCT
GTATTCTCGCGGAGATGCGGCTCGACTACGAAACCTTAATGGCGGCGCTATTACATGACGTCATTGAAGATACACCTGCT
ACATATCAAGATATGGAACAGCTGTTTGGGAAAAGCGTAGCCGAGTTAGTCGAGGGTGTATCTAAACTCGATAAACTGAA
CTTCCGTGACAAGAAAGAAGCCCAGGCGGAAAACTTCCGCAAAATGATCATGGCAATGGTGCAAGACATCCGCGTCATTT
TGATCAAACTGGCTGACCGTACACACAATATGCGCACACTGGGCTCTTTGCGCCCGGATAAACGTCGGCGCATTGCTCGC
GAAACCCTTGAGATATACAGCCCACTCGCCCACCGGCTGGGTATTCATCATTTAAAAACCGAGCTGGAAGAGCTGGGTTT
TGAAGCACTCTATCCCAATCGTTACCGCGTCATTAAAGAAGTGGTAAAAGCCGCGCGCGGTAACCGCAAAGAGATGATTC
AGAAGATCCTCGCGGAGATTGAAGGGCGCTTGACCGAGGCTGGCATTCCTTGCCGGGTCAGTGGCCGTGAGAAGCATCTG
TATTCCATCTACTGCAAGATGCACCTGAAAGAACAGCGCTTCCATTCCATCATGGATATCTATGCTTTTCGGGTTATCGT
CAAAGAAGTCGATACTTGCTACCGCGTGTTGGGGCAGGCTCATAGCCTGTATAAACCCCGCCCTGGGCGCGTAAAAGACT
ATATCGCTATCCCTAAAGCTAACGGCTACCAATCATTACATACGTCATTAATCGGTCCCCATGGCGTACCGGTAGAAGTA
CAAATCCGTACTGAAGATATGGACCAGATGGCCGAAATGGGGGTTGCTGCACACTGGGCTTATAAAGAACAAGGTGAGTC
CGGTACCACGGCTCAAATCCGTGCTCAGCGGTGGATGCAAAGCTTGCTGGAGTTACAACAAAGCGCGGGTAGCTCATTTG
AATTTATTGAAAGCGTGAAATCTGATTTATTCCCTGATGAGATCTACGTTTTCACCCCGGAAGGGCGCATTGTGGAATTA
CCGGCTGGTGCCACACCGGTCGATTTTGCCTATGTTGTGCATACCGATATCGGCCATGCCTGCGTAGGTGCCCGCGTTGA
TCGTCAGCCGTACCCGCTCTCTCAGCCACTGAGTAGTGGCCAGACGGTTGAGATTATTACCGCTCCAGGAGCCAGACCAA
ATGCTGCCTGGCTGAATTTTGTCGTCAGTTCAAAAGCGCGAGCCAAAATACGTCAATTACTGAAAAATCTTAAACGTGAT
GAATCGGTCAGCCTTGGTCGCCGGTTACTGAATCATGCATTAGGAAATGGGCGTAAACTCTCTGATATTTCTGAAGAAAA
TATCAAACACGAGTTGGATAGAATGAAACTGGCTACGGTAGATGACTTGCTGGCAGAAATTGGTTTAGGTAATGCCATGA
GCGTAGTGGTGGCTAAAAACCTATTGGGCGATCCATCAACACTGGGCACCTCTGCTTCTGGTACCCGCAAGTTACCGATC
AAAGGTGCCGATGGCGTACTTATCACGTTTGCCAAGTGCTGCCGTCCAATCCCTGGTGACCCTATTATCGCCCATATCAG
CCCAGGTAAAGGGTTGGTTATTCATCACGAATCTTGTCGGAATATCCGTGGTTACCAGAAAGAGCCTGAGAAATTTATGG
CTGTAGAATGGGACCAAGAGACTGAACAAGAGTTTATTGCCGAAATTAAAGTCGATATGTTTAACCAACAAGGTGCGCTT
GCCAATCTGACCGCCGCGATTAACGCCGCCGAATCCAATATTCAGAGTTTGAATACAGAAGAAAAAGATGGCCGGGTATA
CAGCGCCTTTATTCGCCTGACCACTCGGAACCGGGTCCATCTGGCTAACATTATGCGTAAAATCCGTATCATGCCAGATG
TGGTTAAAGTTAGCCGTAATCGCAATTAA

Upstream 100 bases:

>100_bases
GTTCGTGAGCGCCAAGAGCAGCAAGAGCAGGAAGCCGCAGAGATCCAAGCGGTTACCGCCATTGCTGAAGGTCGTCGTTA
ATTAGACTGCGAGTCTGCCT

Downstream 100 bases:

>100_bases
CGCCTATGAATCCTCAACGCTATGCGCGGATTTGTGACATGCTTGCCACCAGGCAACCCGATTTAACGGTCTGTCTGGAG
CAAGTCCACAAACCTCATAA

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMRLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLNFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHLKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILAEIEGRLTEAGIPCRVSGREKHL
YSIYCKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKANGYQSLHTSLIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEQGESGTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYVVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
ESVSLGRRLLNHALGNGRKLSDISEENIKHELDRMKLATVDDLLAEIGLGNAMSVVVAKNLLGDPSTLGTSASGTRKLPI
KGADGVLITFAKCCRPIPGDPIIAHISPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDQETEQEFIAEIKVDMFNQQGAL
ANLTAAINAAESNIQSLNTEEKDGRVYSAFIRLTTRNRVHLANIMRKIRIMPDVVKVSRNRN

Sequences:

>Translated_702_residues
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMRLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLNFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHLKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILAEIEGRLTEAGIPCRVSGREKHL
YSIYCKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKANGYQSLHTSLIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEQGESGTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYVVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
ESVSLGRRLLNHALGNGRKLSDISEENIKHELDRMKLATVDDLLAEIGLGNAMSVVVAKNLLGDPSTLGTSASGTRKLPI
KGADGVLITFAKCCRPIPGDPIIAHISPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDQETEQEFIAEIKVDMFNQQGAL
ANLTAAINAAESNIQSLNTEEKDGRVYSAFIRLTTRNRVHLANIMRKIRIMPDVVKVSRNRN
>Mature_702_residues
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMRLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLNFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHLKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILAEIEGRLTEAGIPCRVSGREKHL
YSIYCKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKANGYQSLHTSLIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEQGESGTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYVVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
ESVSLGRRLLNHALGNGRKLSDISEENIKHELDRMKLATVDDLLAEIGLGNAMSVVVAKNLLGDPSTLGTSASGTRKLPI
KGADGVLITFAKCCRPIPGDPIIAHISPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDQETEQEFIAEIKVDMFNQQGAL
ANLTAAINAAESNIQSLNTEEKDGRVYSAFIRLTTRNRVHLANIMRKIRIMPDVVKVSRNRN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=702, Percent_Identity=91.4529914529915, Blast_Score=1342, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=680, Percent_Identity=32.9411764705882, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25143118, Length=139, Percent_Identity=33.8129496402878, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24650996, Length=123, Percent_Identity=39.8373983739837, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79169; Mature: 79169

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLNFRDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHLKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILAEIEGRLTEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCEEECCCCCHH
YSIYCKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSLIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEQGESGTTAQIRAQRWMQ
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
SLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYVVHTDIGHA
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCCEEEEEECCCCCH
CVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ESVSLGRRLLNHALGNGRKLSDISEENIKHELDRMKLATVDDLLAEIGLGNAMSVVVAKN
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLGDPSTLGTSASGTRKLPIKGADGVLITFAKCCRPIPGDPIIAHISPGKGLVIHHESCR
HCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEECHHHC
NIRGYQKEPEKFMAVEWDQETEQEFIAEIKVDMFNQQGALANLTAAINAAESNIQSLNTE
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
EKDGRVYSAFIRLTTRNRVHLANIMRKIRIMPDVVKVSRNRN
CCCCCEEEEEEEHHCCCCHHHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLNFRDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHLKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILAEIEGRLTEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCEEECCCCCHH
YSIYCKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSLIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEQGESGTTAQIRAQRWMQ
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
SLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYVVHTDIGHA
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCCEEEEEECCCCCH
CVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ESVSLGRRLLNHALGNGRKLSDISEENIKHELDRMKLATVDDLLAEIGLGNAMSVVVAKN
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLGDPSTLGTSASGTRKLPIKGADGVLITFAKCCRPIPGDPIIAHISPGKGLVIHHESCR
HCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEECHHHC
NIRGYQKEPEKFMAVEWDQETEQEFIAEIKVDMFNQQGALANLTAAINAAESNIQSLNTE
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
EKDGRVYSAFIRLTTRNRVHLANIMRKIRIMPDVVKVSRNRN
CCCCCEEEEEEEHHCCCCHHHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]