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The map label for this gene is rph

Identifier: 170026367

GI number: 170026367

Start: 4590604

End: 4591320

Strand: Reverse

Name: rph

Synonym: YPK_4160

Alternate gene names: 170026367

Gene position: 4591320-4590604 (Counterclockwise)

Preceding gene: 170026378

Following gene: 170026366

Centisome position: 97.91

GC content: 51.32

Gene sequence:

>717_bases
ATGCGTCCAGCAGACCGAGCAGCTCAACAAGTTCGCCCACTGACTTTGACCCGTAATTACACGAAACACGCTGAAGGTTC
AGTGTTGGTTGAATTCGGCGATACCAAAGTATTGTGTACCGCCACAGTTGAAGAAGGTGTTCCACGCTTTCTGAAAGGCC
AGGGCCAAGGGTGGATAACGGCAGAATATGGCATGTTGCCTCGTTCGACCCATAGCCGTAATGCGCGCGAAGCCGCAAAA
GGTAAACAAGGTGGGCGTACTCTTGAAATCCAACGTCTGATCGCCCGTTCTTTACGTGCAGCGGTCGATTTGAAAAAGTT
GGGTGAATTTACCATCACCTTAGACTGCGATGTATTGCAAGCGGATGGTGGCACCCGTACCGCCTCAATCAGTGGTGCTT
GTGTAGCATTGGCTGACGCGCTGAACAAACTGGTTGCTAGTGGTAAATTGAAAGCTAACCCGATGAAGGGCCTGGTTGCT
GCTGTGTCTGTCGGGATCGTTAAAGGCGAGGCTCTTTGCGATCTGGAATACGTAGAAGATTCTGCGGCAGAAACAGATAT
GAATGTGGTGATGATGGAAGATGGCCGGATGATTGAGGTGCAAGGCACCGCTGAAGGTGAGCCGTTCAGTCACGAAGAAT
TATTGGCGTTGCTGGATCTGGCCCGCGGAGGGATAGAAACCATCTTCCAGGCGCAGAAGGCGGCATTGGAATCATAA

Upstream 100 bases:

>100_bases
ATGGGGGGATTATAGCTTCGCAGGTGCGGGCAGGATAGGCATTACATCACTAAGGCCGTATAATGCGCGACCAATATAGA
TTTCAAGCCGGAGAAAGCCC

Downstream 100 bases:

>100_bases
ATTTATTAGGCGACGGATAGTCGCCTTTTTTTACCCGCTAACCTGGAATGCCAAGTGTTCGCGCATTTACCTCAGTTATA
AATCACGTTAGCCATCGGGT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPADRAAQQVRPLTLTRNYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWITAEYGMLPRSTHSRNAREAAK
GKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQADGGTRTASISGACVALADALNKLVASGKLKANPMKGLVA
AVSVGIVKGEALCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLALLDLARGGIETIFQAQKAALES

Sequences:

>Translated_238_residues
MRPADRAAQQVRPLTLTRNYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWITAEYGMLPRSTHSRNAREAAK
GKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQADGGTRTASISGACVALADALNKLVASGKLKANPMKGLVA
AVSVGIVKGEALCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLALLDLARGGIETIFQAQKAALES
>Mature_238_residues
MRPADRAAQQVRPLTLTRNYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWITAEYGMLPRSTHSRNAREAAK
GKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQADGGTRTASISGACVALADALNKLVASGKLKANPMKGLVA
AVSVGIVKGEALCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLALLDLARGGIETIFQAQKAALES

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=223, Percent_Identity=88.7892376681614, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71981632, Length=186, Percent_Identity=27.4193548387097, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_YERP3 (A7FCS9)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001399057.1
- ProteinModelPortal:   A7FCS9
- SMR:   A7FCS9
- STRING:   A7FCS9
- GeneID:   5385287
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_0056
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   YPSE349747:YPSIP31758_0056-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25415; Mature: 25415

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPADRAAQQVRPLTLTRNYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWIT
CCCHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEEEHHHCCHHHHCCCCCEEEE
AEYGMLPRSTHSRNAREAAKGKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQ
EECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGACVALADALNKLVASGKLKANPMKGLVAAVSVGIVKGEALCDLEYVED
CCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLALLDLARGGIETIFQAQKAALES
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPADRAAQQVRPLTLTRNYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWIT
CCCHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEEEHHHCCHHHHCCCCCEEEE
AEYGMLPRSTHSRNAREAAKGKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQ
EECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGACVALADALNKLVASGKLKANPMKGLVAAVSVGIVKGEALCDLEYVED
CCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLALLDLARGGIETIFQAQKAALES
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA