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The map label for this gene is pyrE

Identifier: 170026366

GI number: 170026366

Start: 4589790

End: 4590437

Strand: Reverse

Name: pyrE

Synonym: YPK_4159

Alternate gene names: 170026366

Gene position: 4590437-4589790 (Counterclockwise)

Preceding gene: 170026367

Following gene: 170026362

Centisome position: 97.89

GC content: 50.77

Gene sequence:

>648_bases
ATGAAAGCCTATCAGCGCGAGTTTATCGAGTTTGCGCTTAACAAGCAGGTGTTGAAATTTGGTGAATTTACCCTGAAGTC
TGGGCGCATTAGCCCTTACTTCTTCAATGCCGGGTTGTTTAACACCGGGCTGGATCTGGCGAAACTGGGCCGCTTTTATG
CTGCTGCATTAATGGATTGTGGCGTCGAGTTCGATCTCCTGTTTGGGCCCGCCTACAAAGGTATCCCTATTGCGACAACA
ACCGCCGTGGCATTGGCAGAGCATCATGAGCGCGACGTGCCTTACTGTTTTAACCGTAAAGAAGCGAAAACTCACGGTGA
AGGTGGCAATCTTGTCGGTAGCCCATTACAAGGGCGGGTGATGCTAGTGGATGATGTGATAACCGCCGGTACCGCTATTC
GTGAATCAATGGAGATTATCAATGCGCAGGGCGCGACCCTCGCAGGTGTGATGATTTCACTGGATCGCCAGGAGCGTGGG
CGTGGTGAGATTTCGGCGATTCAGGAAGTTGAACGTGATTATCACTGTAAGGTCATTGCTATCGTGACGCTGAATGATGT
GATTCGCTATCTGGAAGATAAGCCAGAGATGGCTGAGCATTTGGTGGCAGTGCGGCAGTATCGCGAGCAATATGGGGTTA
CCCTTTAG

Upstream 100 bases:

>100_bases
TTACCTCAGTTATAAATCACGTTAGCCATCGGGTAATAAACCAAATTAACACATAACGTTATTAACCAAAGCAGTCACAA
TCTGGAGAGGAGACGTACCA

Downstream 100 bases:

>100_bases
CTGTAACGCTAATAACATGGGGTAATCCCGAATCAAGCTACGCCAAGAGGGCCGTTTGCCCTTTTTGCGTAGCTTGATTC
GCTGTTCTCTCTATGTCTTA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKAYQREFIEFALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGLDLAKLGRFYAAALMDCGVEFDLLFGPAYKGIPIATT
TAVALAEHHERDVPYCFNRKEAKTHGEGGNLVGSPLQGRVMLVDDVITAGTAIRESMEIINAQGATLAGVMISLDRQERG
RGEISAIQEVERDYHCKVIAIVTLNDVIRYLEDKPEMAEHLVAVRQYREQYGVTL

Sequences:

>Translated_215_residues
MKAYQREFIEFALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGLDLAKLGRFYAAALMDCGVEFDLLFGPAYKGIPIATT
TAVALAEHHERDVPYCFNRKEAKTHGEGGNLVGSPLQGRVMLVDDVITAGTAIRESMEIINAQGATLAGVMISLDRQERG
RGEISAIQEVERDYHCKVIAIVTLNDVIRYLEDKPEMAEHLVAVRQYREQYGVTL
>Mature_215_residues
MKAYQREFIEFALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGLDLAKLGRFYAAALMDCGVEFDLLFGPAYKGIPIATT
TAVALAEHHERDVPYCFNRKEAKTHGEGGNLVGSPLQGRVMLVDDVITAGTAIRESMEIINAQGATLAGVMISLDRQERG
RGEISAIQEVERDYHCKVIAIVTLNDVIRYLEDKPEMAEHLVAVRQYREQYGVTL

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=198, Percent_Identity=24.2424242424242, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=85.9154929577465, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17508631, Length=202, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323530, Length=222, Percent_Identity=47.2972972972973, Blast_Score=197, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=43.4389140271493, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_YERP3 (A7FCT0)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001399058.1
- ProteinModelPortal:   A7FCT0
- SMR:   A7FCT0
- STRING:   A7FCT0
- GeneID:   5384616
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_0057
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- ProtClustDB:   PRK00455
- BioCyc:   YPSE349747:YPSIP31758_0057-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23954; Mature: 23954

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 99-99 BINDING 100-100 BINDING 103-103 BINDING 105-105 BINDING 128-128 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYQREFIEFALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGLDLAKLGRFYAAALMDC
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHC
GVEFDLLFGPAYKGIPIATTTAVALAEHHERDVPYCFNRKEAKTHGEGGNLVGSPLQGRV
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCE
MLVDDVITAGTAIRESMEIINAQGATLAGVMISLDRQERGRGEISAIQEVERDYHCKVIA
EEEHHHHHHHHHHHHHHHHHCCCCCEEEHHEEEECHHHCCCCHHHHHHHHHHCCCEEEEE
IVTLNDVIRYLEDKPEMAEHLVAVRQYREQYGVTL
EEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKAYQREFIEFALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGLDLAKLGRFYAAALMDC
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHC
GVEFDLLFGPAYKGIPIATTTAVALAEHHERDVPYCFNRKEAKTHGEGGNLVGSPLQGRV
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCE
MLVDDVITAGTAIRESMEIINAQGATLAGVMISLDRQERGRGEISAIQEVERDYHCKVIA
EEEHHHHHHHHHHHHHHHHHCCCCCEEEHHEEEECHHHCCCCHHHHHHHHHHCCCEEEEE
IVTLNDVIRYLEDKPEMAEHLVAVRQYREQYGVTL
EEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA