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The map label for this gene is metF [H]

Identifier: 170026300

GI number: 170026300

Start: 4523439

End: 4524323

Strand: Reverse

Name: metF [H]

Synonym: YPK_4093

Alternate gene names: 170026300

Gene position: 4524323-4523439 (Counterclockwise)

Preceding gene: 170026301

Following gene: 170026296

Centisome position: 96.48

GC content: 49.72

Gene sequence:

>885_bases
ATGAGTTTTTTTCACGCAAACCAGCGAGAAGCGCTGAACCAGAGTCTGTCTGAGCTACAAGGTCAGATTAATGTTTCTTT
TGAGTTCTTCCCGCCGCGCACCAGCGAGATGGAGGATACCCTGTGGAGCTCGATTGATCGTCTTAGCACGTTGAAACCTA
AATTTGTTTCCGTGACCTATGGGGCGAACTCGGGTGAGCGTGACCGTACTCACAGCATCATCAAAGGGATTAAGGAGCGC
ACAGGCCTGGAAGCTGCGCCGCATCTGACCTGTATTGATGCTTCTCCGACTCAGTTGCGTGATATCGCGATTGACTACTG
GAATAGCGGTATCCGACATATCGTTGCCTTACGGGGTGATTTGCCGCCAAACAGCGGAAAACCCGAAATGTATGCCTGCG
ATTTGGTGGGTTTGCTAAAAGATGTGGGTGATTTTGATATCTCCGTGGCGGCGTACCCAGAAATTCATCCAGAAGCGAAA
AGCGCCCAAGCTGACTTGATCAATCTGAAACGTAAGATTGATGCAGGTGCTAACCGGGCCATCACCCAATTTTTCTTTGA
CGTTGAGAGTTACCTGCGTTTTCGTGACCGCTGTGTTGCCACCGGGATTGACGTTGAGATCGTACCGGGCATTTTGCCGG
TTTCTAATTTTAAGCAGTTACAGCGTTTTGCCACCATGACCAATGTACGGGTCCCAAATTGGATGACATCTATTTTTGAC
GGTCTGGATAATGACCCAGAGACGCGCAAAATGGTGGGGGCATCGGTCGCGATGGACATGGTGAAGATCCTCAGCCGCGA
AGGGGTGAAGGATTTCCATTTTTATACTTTGAACCGGGCTGAGTTGAGTTATGCGATTTGTCATACCCTTGGGGTGAGGC
CTTAG

Upstream 100 bases:

>100_bases
CCAACTTCCGTCATTTTCTATTTAGACGTCTAAACGTATAGACGCTCATAAATAATGACAAATAACATCAGCCGTCATAA
TGGCAATGGAGTAACAGGGT

Downstream 100 bases:

>100_bases
TTTTTTAAAGGGGGGAACGGTTTTAATCTCCTGGTGGTTTGGTGGTTTGGTGGTTTGGTGGTTTGGTGGTTTGGTGGTTT
GGTGGTTTGGTGGTTTGGTG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSFFHANQREALNQSLSELQGQINVSFEFFPPRTSEMEDTLWSSIDRLSTLKPKFVSVTYGANSGERDRTHSIIKGIKER
TGLEAAPHLTCIDASPTQLRDIAIDYWNSGIRHIVALRGDLPPNSGKPEMYACDLVGLLKDVGDFDISVAAYPEIHPEAK
SAQADLINLKRKIDAGANRAITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPNWMTSIFD
GLDNDPETRKMVGASVAMDMVKILSREGVKDFHFYTLNRAELSYAICHTLGVRP

Sequences:

>Translated_294_residues
MSFFHANQREALNQSLSELQGQINVSFEFFPPRTSEMEDTLWSSIDRLSTLKPKFVSVTYGANSGERDRTHSIIKGIKER
TGLEAAPHLTCIDASPTQLRDIAIDYWNSGIRHIVALRGDLPPNSGKPEMYACDLVGLLKDVGDFDISVAAYPEIHPEAK
SAQADLINLKRKIDAGANRAITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPNWMTSIFD
GLDNDPETRKMVGASVAMDMVKILSREGVKDFHFYTLNRAELSYAICHTLGVRP
>Mature_293_residues
SFFHANQREALNQSLSELQGQINVSFEFFPPRTSEMEDTLWSSIDRLSTLKPKFVSVTYGANSGERDRTHSIIKGIKERT
GLEAAPHLTCIDASPTQLRDIAIDYWNSGIRHIVALRGDLPPNSGKPEMYACDLVGLLKDVGDFDISVAAYPEIHPEAKS
AQADLINLKRKIDAGANRAITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPNWMTSIFDG
LDNDPETRKMVGASVAMDMVKILSREGVKDFHFYTLNRAELSYAICHTLGVRP

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=300, Percent_Identity=34.6666666666667, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=86.3945578231292, Blast_Score=548, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI25147441, Length=296, Percent_Identity=30.4054054054054, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25147436, Length=296, Percent_Identity=30.4054054054054, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=32.6530612244898, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321313, Length=291, Percent_Identity=31.2714776632302, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32976; Mature: 32845

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFHANQREALNQSLSELQGQINVSFEFFPPRTSEMEDTLWSSIDRLSTLKPKFVSVTY
CCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
GANSGERDRTHSIIKGIKERTGLEAAPHLTCIDASPTQLRDIAIDYWNSGIRHIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCHHHEEEEEECC
LPPNSGKPEMYACDLVGLLKDVGDFDISVAAYPEIHPEAKSAQADLINLKRKIDAGANRA
CCCCCCCCCEEHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPNWMTSIFD
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHC
GLDNDPETRKMVGASVAMDMVKILSREGVKDFHFYTLNRAELSYAICHTLGVRP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SFFHANQREALNQSLSELQGQINVSFEFFPPRTSEMEDTLWSSIDRLSTLKPKFVSVTY
CCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
GANSGERDRTHSIIKGIKERTGLEAAPHLTCIDASPTQLRDIAIDYWNSGIRHIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCHHHEEEEEECC
LPPNSGKPEMYACDLVGLLKDVGDFDISVAAYPEIHPEAKSAQADLINLKRKIDAGANRA
CCCCCCCCCEEHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPNWMTSIFD
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHC
GLDNDPETRKMVGASVAMDMVKILSREGVKDFHFYTLNRAELSYAICHTLGVRP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA