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The map label for this gene is ascD

Identifier: 170025409

GI number: 170025409

Start: 3490801

End: 3491790

Strand: Reverse

Name: ascD

Synonym: YPK_3192

Alternate gene names: 170025409

Gene position: 3491790-3490801 (Counterclockwise)

Preceding gene: 170025410

Following gene: 170025408

Centisome position: 74.46

GC content: 37.37

Gene sequence:

>990_bases
ATGTCATTAAATGTTAAGCTGCATCCATCAGGTATTATTTTTACTTCCGATGGAACATCTACAATATTAGATGCGGCTCT
GGATAGTAATATACATATTGAATACAGCTGCAAAGATGGAACCTGTGGTTCTTGTAAGGCAATATTGATTTCTGGTGAAG
TAGACAGTGCGGAAAATACCTTTTTAACTGAGGAAGATGTTGCTAAAGGTGCAATCCTCACTTGTTGCTCTAAGGCTAAA
TCTGATATTGAGTTAGATGTTAATTATTATCCAGAGTTAAGTCATATACAAAAAAAAACTTATCCATGTAAATTAGATAG
CATTGAATTTATTGGTGAAGATATTGCCATTCTCTCCTTACGTTTGCCACCAACGGCCAAAATACAGTATCTGGCGGGCC
AATACATTGATTTAATTATTAATGGACAGCGCCGTAGTTACTCTATTGCTAATGCTCCAGGTGGTAATGGCAATATCGAA
TTACACGTACGTAAAGTTGTTAATGGTGTATTCAGCAACATCATTTTTAATGAGTTAAAATTACAGCAACTTTTACGAAT
TGAAGGTCCTCAAGGGACCTTTTTCGTTCGTGAAGATAATCTCCCTATTGTTTTTCTTGCTGGTGGAACAGGTTTTGCAC
CAGTGAAATCAATGGTTGAGGCGTTGATCAATAAAAATGACCAACGGCAGGTTCATATCTATTGGGGAATGCCAGCAGGG
CATAATTTCTATTCTGACATTGCCAATGAGTGGGCTATAAAACACCCTAACATTCATTATGTTCCTGTTGTATCAGGCGA
TGATAGTACTTGGACCGGAGCCACTGGTTTTGTACATCAAGCGGTGCTTGAAGATATACCCGATCTCAGCTTATTTAATG
TTTATGCCTGTGGTTCATTAGCGATGATTACTGCTGCTCGTAATGATTTCATCAATCATGGATTAGCTGAAAATAAATTT
TTCTCTGATGCCTTTGTGCCATCAAAATAA

Upstream 100 bases:

>100_bases
ATTTGCTTTTTTGTTTCTATTTTAGTCACTGAATTTTTTTGACTTAAGTTTGTATTGAGTACGTCTAATAAACCTTACAT
AATATATTTGGACTTATTTT

Downstream 100 bases:

>100_bases
CTTTGAGAGATCAAAGTAATACGTAATGGAGATTCAAGTGAAAGCAGTCATTTTGGCCGGAGGCCTTGGAACCCGTCTTA
GTGAAGAGACCGTAGTAAAA

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSLNVKLHPSGIIFTSDGTSTILDAALDSNIHIEYSCKDGTCGSCKAILISGEVDSAENTFLTEEDVAKGAILTCCSKAK
SDIELDVNYYPELSHIQKKTYPCKLDSIEFIGEDIAILSLRLPPTAKIQYLAGQYIDLIINGQRRSYSIANAPGGNGNIE
LHVRKVVNGVFSNIIFNELKLQQLLRIEGPQGTFFVREDNLPIVFLAGGTGFAPVKSMVEALINKNDQRQVHIYWGMPAG
HNFYSDIANEWAIKHPNIHYVPVVSGDDSTWTGATGFVHQAVLEDIPDLSLFNVYACGSLAMITAARNDFINHGLAENKF
FSDAFVPSK

Sequences:

>Translated_329_residues
MSLNVKLHPSGIIFTSDGTSTILDAALDSNIHIEYSCKDGTCGSCKAILISGEVDSAENTFLTEEDVAKGAILTCCSKAK
SDIELDVNYYPELSHIQKKTYPCKLDSIEFIGEDIAILSLRLPPTAKIQYLAGQYIDLIINGQRRSYSIANAPGGNGNIE
LHVRKVVNGVFSNIIFNELKLQQLLRIEGPQGTFFVREDNLPIVFLAGGTGFAPVKSMVEALINKNDQRQVHIYWGMPAG
HNFYSDIANEWAIKHPNIHYVPVVSGDDSTWTGATGFVHQAVLEDIPDLSLFNVYACGSLAMITAARNDFINHGLAENKF
FSDAFVPSK
>Mature_328_residues
SLNVKLHPSGIIFTSDGTSTILDAALDSNIHIEYSCKDGTCGSCKAILISGEVDSAENTFLTEEDVAKGAILTCCSKAKS
DIELDVNYYPELSHIQKKTYPCKLDSIEFIGEDIAILSLRLPPTAKIQYLAGQYIDLIINGQRRSYSIANAPGGNGNIEL
HVRKVVNGVFSNIIFNELKLQQLLRIEGPQGTFFVREDNLPIVFLAGGTGFAPVKSMVEALINKNDQRQVHIYWGMPAGH
NFYSDIANEWAIKHPNIHYVPVVSGDDSTWTGATGFVHQAVLEDIPDLSLFNVYACGSLAMITAARNDFINHGLAENKFF
SDAFVPSK

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI2367314, Length=230, Percent_Identity=30.8695652173913, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322146, Length=194, Percent_Identity=28.8659793814433, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASCD_YERPE (P68641)

Other databases:

- EMBL:   AJ251713
- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0378
- RefSeq:   NP_668397.1
- RefSeq:   NP_992202.1
- RefSeq:   YP_002348024.1
- ProteinModelPortal:   P68641
- SMR:   P68641
- GeneID:   1146014
- GeneID:   1175931
- GeneID:   2764213
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3116
- KEGG:   ypk:y1067
- KEGG:   ypm:YP_0814
- HOGENOM:   HBG680293
- OMA:   PGGLFTD
- ProtClustDB:   PRK07609
- BioCyc:   YPES187410:Y1067-MONOMER
- BioCyc:   YPES214092:YPO3116-MONOMER
- GO:   GO:0006810
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR00410

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1; SSF54292 Ferredoxin; SSF63380 Riboflavin_synthase_like_b-brl

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 36047; Mature: 35916

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNVKLHPSGIIFTSDGTSTILDAALDSNIHIEYSCKDGTCGSCKAILISGEVDSAENT
CCEEEEECCCEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCC
FLTEEDVAKGAILTCCSKAKSDIELDVNYYPELSHIQKKTYPCKLDSIEFIGEDIAILSL
EECHHHHHCCHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHHCCCCEEEEEE
RLPPTAKIQYLAGQYIDLIINGQRRSYSIANAPGGNGNIELHVRKVVNGVFSNIIFNELK
ECCCCCEEEEECCCEEEEEEECCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
LQQLLRIEGPQGTFFVREDNLPIVFLAGGTGFAPVKSMVEALINKNDQRQVHIYWGMPAG
HHHHHEECCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCC
HNFYSDIANEWAIKHPNIHYVPVVSGDDSTWTGATGFVHQAVLEDIPDLSLFNVYACGSL
CHHHHHHHHHCEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCE
AMITAARNDFINHGLAENKFFSDAFVPSK
EEEEEHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLNVKLHPSGIIFTSDGTSTILDAALDSNIHIEYSCKDGTCGSCKAILISGEVDSAENT
CEEEEECCCEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCC
FLTEEDVAKGAILTCCSKAKSDIELDVNYYPELSHIQKKTYPCKLDSIEFIGEDIAILSL
EECHHHHHCCHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHHCCCCEEEEEE
RLPPTAKIQYLAGQYIDLIINGQRRSYSIANAPGGNGNIELHVRKVVNGVFSNIIFNELK
ECCCCCEEEEECCCEEEEEEECCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
LQQLLRIEGPQGTFFVREDNLPIVFLAGGTGFAPVKSMVEALINKNDQRQVHIYWGMPAG
HHHHHEECCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCC
HNFYSDIANEWAIKHPNIHYVPVVSGDDSTWTGATGFVHQAVLEDIPDLSLFNVYACGSL
CHHHHHHHHHCEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCE
AMITAARNDFINHGLAENKFFSDAFVPSK
EEEEEHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430