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The map label for this gene is rfbF [H]

Identifier: 170025408

GI number: 170025408

Start: 3489990

End: 3490763

Strand: Reverse

Name: rfbF [H]

Synonym: YPK_3191

Alternate gene names: 170025408

Gene position: 3490763-3489990 (Counterclockwise)

Preceding gene: 170025409

Following gene: 170025407

Centisome position: 74.44

GC content: 43.93

Gene sequence:

>774_bases
GTGAAAGCAGTCATTTTGGCCGGAGGCCTTGGAACCCGTCTTAGTGAAGAGACCGTAGTAAAACCAAAACCAATGGTAGA
GATTGGTGGGAAGCCTATTCTCTGGCATATTATGAAACTTTACTCTTCTTATGGCATCAATGATTTCGTTATTTGCTGTG
GTTATAAAGGCTATGTCATTAAAGAATATTTTGCGAATTACTTTATGCACATGTCGGATATTACCTTCTGCATGCGTGAT
AATGAAATGATAGTACACCAAAAGCGAGTCGAACCCTGGAATGTCACGTTAGTAGACACCGGCGAAGATTCTATGACCGG
TGGCCGACTGAGGCGTGTAAAAGATTACGTCAAAGACGATGAGGCTTTCTGTTTCACCTATGGTGATGGTGTGAGTGACG
TCAATATTGCTGAATTAATTGAATTCCATAAGAGCCACGGCAAGCAAGCCACATTAACGGCAACCTATCCCCCAGGCCGT
TTTGGTGCGCTGGATATTAAAGATAAACAAGTACGTAGTTTTAAAGAAAAACCAAAAGGCGATGGCGCATTGATCAACGG
CGGTTATTTTGTTTTGTCGCCTAAAGTTATCGATCTGATCGATGGTGATAAGTCTACTTGGGAGCAGGAACCTTTAATGA
CATTAGCTGCGCAGGGGGAGTTGATGGCGTTTGAACATGCCGGGTTCTGGCAGCCAATGGATACGTTGCGTGACAAGATC
TATCTGCATGAACTATGGGAAGAAGGCAGGGCACCTTGGAAGGTATGGGAATAA

Upstream 100 bases:

>100_bases
TTCATCAATCATGGATTAGCTGAAAATAAATTTTTCTCTGATGCCTTTGTGCCATCAAAATAACTTTGAGAGATCAAAGT
AATACGTAATGGAGATTCAA

Downstream 100 bases:

>100_bases
CAAAATGATTAATAATAGTTTCTGGCAAGGTAAACGGGTTTTTGTAACAGGCCATACTGGGTTTAAAGGTGGCTGGTTGA
GTTTATGGTTGCAAACCATG

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKAVILAGGLGTRLSEETVVKPKPMVEIGGKPILWHIMKLYSSYGINDFVICCGYKGYVIKEYFANYFMHMSDITFCMRD
NEMIVHQKRVEPWNVTLVDTGEDSMTGGRLRRVKDYVKDDEAFCFTYGDGVSDVNIAELIEFHKSHGKQATLTATYPPGR
FGALDIKDKQVRSFKEKPKGDGALINGGYFVLSPKVIDLIDGDKSTWEQEPLMTLAAQGELMAFEHAGFWQPMDTLRDKI
YLHELWEEGRAPWKVWE

Sequences:

>Translated_257_residues
MKAVILAGGLGTRLSEETVVKPKPMVEIGGKPILWHIMKLYSSYGINDFVICCGYKGYVIKEYFANYFMHMSDITFCMRD
NEMIVHQKRVEPWNVTLVDTGEDSMTGGRLRRVKDYVKDDEAFCFTYGDGVSDVNIAELIEFHKSHGKQATLTATYPPGR
FGALDIKDKQVRSFKEKPKGDGALINGGYFVLSPKVIDLIDGDKSTWEQEPLMTLAAQGELMAFEHAGFWQPMDTLRDKI
YLHELWEEGRAPWKVWE
>Mature_257_residues
MKAVILAGGLGTRLSEETVVKPKPMVEIGGKPILWHIMKLYSSYGINDFVICCGYKGYVIKEYFANYFMHMSDITFCMRD
NEMIVHQKRVEPWNVTLVDTGEDSMTGGRLRRVKDYVKDDEAFCFTYGDGVSDVNIAELIEFHKSHGKQATLTATYPPGR
FGALDIKDKQVRSFKEKPKGDGALINGGYFVLSPKVIDLIDGDKSTWEQEPLMTLAAQGELMAFEHAGFWQPMDTLRDKI
YLHELWEEGRAPWKVWE

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=246, Percent_Identity=27.6422764227642, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI11761619, Length=246, Percent_Identity=27.6422764227642, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI133931050, Length=248, Percent_Identity=31.0483870967742, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320148, Length=268, Percent_Identity=32.4626865671642, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355443, Length=259, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24644084, Length=259, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 29293; Mature: 29293

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILAGGLGTRLSEETVVKPKPMVEIGGKPILWHIMKLYSSYGINDFVICCGYKGYVI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHH
KEYFANYFMHMSDITFCMRDNEMIVHQKRVEPWNVTLVDTGEDSMTGGRLRRVKDYVKDD
HHHHHHHHHHHHHEEEEEECCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCC
EAFCFTYGDGVSDVNIAELIEFHKSHGKQATLTATYPPGRFGALDIKDKQVRSFKEKPKG
CEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCEECCCHHHHHHHHHCCCC
DGALINGGYFVLSPKVIDLIDGDKSTWEQEPLMTLAAQGELMAFEHAGFWQPMDTLRDKI
CCEEEECCEEEECCEEEEEECCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHH
YLHELWEEGRAPWKVWE
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAVILAGGLGTRLSEETVVKPKPMVEIGGKPILWHIMKLYSSYGINDFVICCGYKGYVI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHH
KEYFANYFMHMSDITFCMRDNEMIVHQKRVEPWNVTLVDTGEDSMTGGRLRRVKDYVKDD
HHHHHHHHHHHHHEEEEEECCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCC
EAFCFTYGDGVSDVNIAELIEFHKSHGKQATLTATYPPGRFGALDIKDKQVRSFKEKPKG
CEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCEECCCHHHHHHHHHCCCC
DGALINGGYFVLSPKVIDLIDGDKSTWEQEPLMTLAAQGELMAFEHAGFWQPMDTLRDKI
CCEEEECCEEEECCEEEEEECCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHH
YLHELWEEGRAPWKVWE
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]