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The map label for this gene is fcl [H]

Identifier: 170025399

GI number: 170025399

Start: 3479306

End: 3480271

Strand: Reverse

Name: fcl [H]

Synonym: YPK_3182

Alternate gene names: 170025399

Gene position: 3480271-3479306 (Counterclockwise)

Preceding gene: 170025400

Following gene: 170025398

Centisome position: 74.22

GC content: 41.51

Gene sequence:

>966_bases
ATGGATAAGAAACGCGTATTTATTGCCGGGCATCGTGGCATGGTGGGTTCTGCTATTGTACGTCAACTTGAAAACCGTAA
TGATATTGAATTGATCATTAGGGATCGTACTGAACTTGACCTCATGTCTCAATCCGCTGTGCAAAAATTTTTTGCTACTG
AAAAAATTGATGAAATCTATTTGGCTGCGGCAAAAGTGGGGGGGATTCAGGCCAATAATAATTATCCGGCAGAGTTCATC
TACCAAAACTTAATGATCGAGTGCAATATTATTCACGCGGCTCATTTAGCTGGCATTCAAAAATTATTATTTTTGGGGTC
TTCTTGTATTTATCCAAAATTGGCTGCACAACCAATGACAGAGGAGGCTCTGTTAACTGGCGTCTTGGAACCAACGAATG
AACCTTATGCCATCGCCAAGATAGCCGGTATCAAACTGTGCGAATCTTATAATCGTCAATATGGTCGCGATTATCGCAGT
GTTATGCCAACCAACCTTTATGGTGAAAATGACAATTTTCACCCCGAAAATTCCCATGTCATTCCTGCCTTATTACGTCG
CTTCCATGAGGCTAAAATTCGTAATGATAAGGAAATGGTTGTGTGGGGAACGGGTAAACCAATGCGTGAGTTCCTGCATG
TAGATGATATGGCTGCTGCCAGTGTGCATGTCATGGAGCTGTCTGATCAAATTTATCAAACCAATACTCAACCAATGCTT
TCGCATATTAATGTCGGAACGGGTGTGGATTGCACTATTCGTGAATTGGCAGAAACTATGGCTAAAGTTGTTGGTTTCAC
CGGAAATTTAGTTTTTGATTCAACTAAGCCGGACGGAACACCACGAAAATTGATGGACGTAAGCCGCTTGGCTAAACTCG
GGTGGTGTTATCAGATTTCGCTTGAAGTAGGTTTAACGATGACTTATCAATGGTTCTTGGCTCATCAGAATAACTTCAGA
AAATAG

Upstream 100 bases:

>100_bases
GTGCTGAAATGGTCGCTAGTGACTTAGAGCAGGCTAAACAGCATGCATTGTTAAAGGCTAATGGCTTTGACGTATCCATT
TCGTTGGAGAATTAATACAA

Downstream 100 bases:

>100_bases
TTTTATTTGCTATTTGCTATTTGCTATTTGCTATTTGCTATTTGCTATTTGCTATTTGCTATTTGGATATGGCGACTCTT
AAGTTGGTTAATTAAGTTTT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIYLAAAKVGGIQANNNYPAEFI
YQNLMIECNIIHAAHLAGIQKLLFLGSSCIYPKLAAQPMTEEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGENDNFHPENSHVIPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQISLEVGLTMTYQWFLAHQNNFR
K

Sequences:

>Translated_321_residues
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIYLAAAKVGGIQANNNYPAEFI
YQNLMIECNIIHAAHLAGIQKLLFLGSSCIYPKLAAQPMTEEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGENDNFHPENSHVIPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQISLEVGLTMTYQWFLAHQNNFR
K
>Mature_321_residues
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIYLAAAKVGGIQANNNYPAEFI
YQNLMIECNIIHAAHLAGIQKLLFLGSSCIYPKLAAQPMTEEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGENDNFHPENSHVIPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQISLEVGLTMTYQWFLAHQNNFR
K

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=289, Percent_Identity=29.0657439446367, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI7657641, Length=348, Percent_Identity=22.7011494252874, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788365, Length=320, Percent_Identity=75.3125, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=22.9461756373938, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554428, Length=322, Percent_Identity=28.2608695652174, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI19922778, Length=327, Percent_Identity=30.8868501529052, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36289; Mature: 36289

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIY
CCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LAAAKVGGIQANNNYPAEFIYQNLMIECNIIHAAHLAGIQKLLFLGSSCIYPKLAAQPMT
HHHHHHCCEECCCCCCHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHCCCCC
EEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGENDNFHPENSHV
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCC
IPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCHHHHHEEEEHHHHHHHHCCCCHHH
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQIS
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
LEVGLTMTYQWFLAHQNNFRK
EEECEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIY
CCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LAAAKVGGIQANNNYPAEFIYQNLMIECNIIHAAHLAGIQKLLFLGSSCIYPKLAAQPMT
HHHHHHCCEECCCCCCHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHCCCCC
EEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGENDNFHPENSHV
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCC
IPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCHHHHHEEEEHHHHHHHHCCCCHHH
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQIS
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
LEVGLTMTYQWFLAHQNNFRK
EEECEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]