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The map label for this gene is gmd [H]

Identifier: 170025400

GI number: 170025400

Start: 3480277

End: 3481398

Strand: Reverse

Name: gmd [H]

Synonym: YPK_3183

Alternate gene names: 170025400

Gene position: 3481398-3480277 (Counterclockwise)

Preceding gene: 170025401

Following gene: 170025399

Centisome position: 74.24

GC content: 41.0

Gene sequence:

>1122_bases
ATGACTAAGATTGCGCTCATTACTGGTATTACAGGACAAGATGGCTCTTATTTGGCTGAGTTTTTATTAGAAAAGGGTTA
TGAGGTTCATGGTATTAAGCGCCGAGCTTCATCTTTTAATACCAGCCGCATTGATCATATTTATCAAGATCGCCATGAGA
CGAATCCACGCTTCTTTTTACATTATGGTGACTTGACTGACACGTCGAATTTAATTCGTTTGGTCCAGGAAATTCAGCCT
GATGAGATTTATAATTTGGGTGCACAATCTCATGTGGCTGTTTCATTTGAATCACCGGAATATACTGCCGATGTTGATGC
CATGGGGACACTGCGGTTGCTAGAGGCCATTCGTATCAATGGGCTTGAAAAGAAGACTCGTTTCTATCAAGCCTCAACCT
CAGAGCTTTATGGTTTAGTGCAGGAAACGCCTCAGCGTGAAACAACGCCATTCTATCCGCGTTCTCCTTATGCTGTTGCC
AAAATGTATGCTTACTGGATTACAGTAAATTACCGTGAATCATATGGAATGTATGCTTGTAACGGAATTTTATTTAACCA
TGAGTCTCCTCGTCGTGGTGAAACATTTGTTACTCGGAAGATTACCCGTGCTGTTGCGAATATTGCATTAGGTCTAGAAA
AATGTCTCTATTTAGGCAATATAGATTCGCTACGTGACTGGGGCCATGCGAAAGATTATGTGCGTATGCAATGGATGATG
TTGCAACAAGATAAGCCAGAAGATTTTGTTATTGCAACAGGCAAGCAAATTACCGTTCGTGAGTTTGTACGTATGTCAGC
AAGAGAAGCAGGGATTGAATTGGAATTTAGTGGAGAAGGCGTTGAAGAGGTTGCCACTGTTGTTGCTATTAATGGTAACC
ATATTTCTTCTGTCAATATTGGAGATGTAATAGTCCGTGTTGATCCACGTTATTTCCGTCCTGCTGAAGTTGAAACATTA
TTAGGCGATCCAACTAAGGCTAAAAAAGTATTAGGCTGGGTACCTGAAATTACGGTTGAAGAAATGTGTGCTGAAATGGT
CGCTAGTGACTTAGAGCAGGCTAAACAGCATGCATTGTTAAAGGCTAATGGCTTTGACGTATCCATTTCGTTGGAGAATT
AA

Upstream 100 bases:

>100_bases
CTATGGCATGAAGCAAGCGGCTAAGTTCTCATGGGATAAGTGTTATCAAGAAACCTTAGATGTTTATAAAGAATTGAACA
AATAAAATGGATTACAATAT

Downstream 100 bases:

>100_bases
TACAAATGGATAAGAAACGCGTATTTATTGCCGGGCATCGTGGCATGGTGGGTTCTGCTATTGTACGTCAACTTGAAAAC
CGTAATGATATTGAATTGAT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTKIALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDRHETNPRFFLHYGDLTDTSNLIRLVQEIQP
DEIYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRINGLEKKTRFYQASTSELYGLVQETPQRETTPFYPRSPYAVA
KMYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAVANIALGLEKCLYLGNIDSLRDWGHAKDYVRMQWMM
LQQDKPEDFVIATGKQITVREFVRMSAREAGIELEFSGEGVEEVATVVAINGNHISSVNIGDVIVRVDPRYFRPAEVETL
LGDPTKAKKVLGWVPEITVEEMCAEMVASDLEQAKQHALLKANGFDVSISLEN

Sequences:

>Translated_373_residues
MTKIALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDRHETNPRFFLHYGDLTDTSNLIRLVQEIQP
DEIYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRINGLEKKTRFYQASTSELYGLVQETPQRETTPFYPRSPYAVA
KMYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAVANIALGLEKCLYLGNIDSLRDWGHAKDYVRMQWMM
LQQDKPEDFVIATGKQITVREFVRMSAREAGIELEFSGEGVEEVATVVAINGNHISSVNIGDVIVRVDPRYFRPAEVETL
LGDPTKAKKVLGWVPEITVEEMCAEMVASDLEQAKQHALLKANGFDVSISLEN
>Mature_372_residues
TKIALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDRHETNPRFFLHYGDLTDTSNLIRLVQEIQPD
EIYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRINGLEKKTRFYQASTSELYGLVQETPQRETTPFYPRSPYAVAK
MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAVANIALGLEKCLYLGNIDSLRDWGHAKDYVRMQWMML
QQDKPEDFVIATGKQITVREFVRMSAREAGIELEFSGEGVEEVATVVAINGNHISSVNIGDVIVRVDPRYFRPAEVETLL
GDPTKAKKVLGWVPEITVEEMCAEMVASDLEQAKQHALLKANGFDVSISLEN

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=352, Percent_Identity=59.9431818181818, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1788366, Length=373, Percent_Identity=79.6246648793566, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=28.9398280802292, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788353, Length=205, Percent_Identity=27.3170731707317, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=351, Percent_Identity=65.2421652421652, Blast_Score=471, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI133901790, Length=363, Percent_Identity=62.534435261708, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17539424, Length=363, Percent_Identity=62.534435261708, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901788, Length=363, Percent_Identity=62.534435261708, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901786, Length=363, Percent_Identity=62.534435261708, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17539422, Length=363, Percent_Identity=62.534435261708, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24158427, Length=358, Percent_Identity=59.2178770949721, Blast_Score=445, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 42258; Mature: 42127

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDRHETNPRFFL
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHHHHCCCCCEEEE
HYGDLTDTSNLIRLVQEIQPDEIYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRIN
EECCCCCHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
GLEKKTRFYQASTSELYGLVQETPQRETTPFYPRSPYAVAKMYAYWITVNYRESYGMYAC
CCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEEEEEEECCCCCEEEE
NGILFNHESPRRGETFVTRKITRAVANIALGLEKCLYLGNIDSLRDWGHAKDYVRMQWMM
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHH
LQQDKPEDFVIATGKQITVREFVRMSAREAGIELEFSGEGVEEVATVVAINGNHISSVNI
HCCCCCCCEEEECCCEEHHHHHHHHHHHHCCEEEEECCCCHHHEEEEEEECCCEEEEEEC
GDVIVRVDPRYFRPAEVETLLGDPTKAKKVLGWVPEITVEEMCAEMVASDLEQAKQHALL
CEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEE
KANGFDVSISLEN
EECCEEEEEEECC
>Mature Secondary Structure 
TKIALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDRHETNPRFFL
CEEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHHHHCCCCCEEEE
HYGDLTDTSNLIRLVQEIQPDEIYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRIN
EECCCCCHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
GLEKKTRFYQASTSELYGLVQETPQRETTPFYPRSPYAVAKMYAYWITVNYRESYGMYAC
CCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEEEEEEECCCCCEEEE
NGILFNHESPRRGETFVTRKITRAVANIALGLEKCLYLGNIDSLRDWGHAKDYVRMQWMM
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHH
LQQDKPEDFVIATGKQITVREFVRMSAREAGIELEFSGEGVEEVATVVAINGNHISSVNI
HCCCCCCCEEEECCCEEHHHHHHHHHHHHCCEEEEECCCCHHHEEEEEEECCCEEEEEEC
GDVIVRVDPRYFRPAEVETLLGDPTKAKKVLGWVPEITVEEMCAEMVASDLEQAKQHALL
CEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEE
KANGFDVSISLEN
EECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]