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The map label for this gene is hemK [H]

Identifier: 170024409

GI number: 170024409

Start: 2416889

End: 2417719

Strand: Reverse

Name: hemK [H]

Synonym: YPK_2178

Alternate gene names: 170024409

Gene position: 2417719-2416889 (Counterclockwise)

Preceding gene: 170024410

Following gene: 170024408

Centisome position: 51.56

GC content: 53.07

Gene sequence:

>831_bases
ATGAATTATCAGCATTGGCTGTCGTTGGCGGCAGCCCGTTTTACCCACAGTGACAGCCCTAAGCGTGATGCTGAAATCCT
ACTGAGTTTTGTGACAGGTAAGGCCCGGACCTATCTGTTGGCTTTTGGTGAAACGGAAATAACGGCAGAACAACTACTGT
GGCTCGAAACACTGGCGAACCGTCGGGAGCAAGGTGAACCTATTGCTTATCTGGTTGGGGAACGCGAGTTCTGGTCTTTA
CCGCTGAGTGTTTCCTCTGCAACGCTTATCCCACGGCCTGACACCGAATGTCTGGTGGAGCAGGCGCTGGCACGTTTGCC
CTCAATGCCTTGTCGTATTTTGGACCTGGGGACGGGTACTGGTGCAATTGCGTTGGCACTGGCCAGTGAACGGCGTGATT
GCGCGGTTATCGCCGTAGATATCAACGCTGATGCTGTCGCTTTGGCCCGCCATAATGCAAAAAAACTCGCGATAGATAAT
GTGTGTTTTTTGCAAGGCAGTTGGTTTGAGCCAGTGAGCGGGCGTTTTGCGCTTATCGCCAGTAACCCCCCTTATATTGA
TGCCAATGACCCTCATCTTAATGAAGGCGATGTGCGTTATGAGCCGCATAGTGCATTGGTTGCGGCCGCTGAGGGGATGG
CCGATCTGGCCGCGATTGTCAGCCAGGCACCGGGCTACTTGGAACCCGGCGGTTGGCTCATGCTAGAGCATGGCTGGCAG
CAGGCCAACGCAGTGCAAGAACGGCTTAAAAATTCGGGATTTAGTGCGGTAATGACCTGTAAAGATTATGGCAATAATGA
CCGGGTGACATTGGGGCAATGGTCCGTGTAA

Upstream 100 bases:

>100_bases
GGCTTGATGAAGTGATGGAAGGTAAACTGGATATGCTCATCCAGCCCATTGTGCAGGAATATCAAGCTGACCAACTGTCT
GCATTGTCAGAGCAAGACTA

Downstream 100 bases:

>100_bases
CCACGCGCAGCTAATAGCGCTGCAACATCAAGTAAAAAGAGATAAAACAAGGAATCGATAGTCATGTGGGTTGATTACAT
CGCCATGAAATATTTGCATT

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLANRREQGEPIAYLVGEREFWSL
PLSVSSATLIPRPDTECLVEQALARLPSMPCRILDLGTGTGAIALALASERRDCAVIAVDINADAVALARHNAKKLAIDN
VCFLQGSWFEPVSGRFALIASNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLEPGGWLMLEHGWQ
QANAVQERLKNSGFSAVMTCKDYGNNDRVTLGQWSV

Sequences:

>Translated_276_residues
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLANRREQGEPIAYLVGEREFWSL
PLSVSSATLIPRPDTECLVEQALARLPSMPCRILDLGTGTGAIALALASERRDCAVIAVDINADAVALARHNAKKLAIDN
VCFLQGSWFEPVSGRFALIASNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLEPGGWLMLEHGWQ
QANAVQERLKNSGFSAVMTCKDYGNNDRVTLGQWSV
>Mature_276_residues
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLANRREQGEPIAYLVGEREFWSL
PLSVSSATLIPRPDTECLVEQALARLPSMPCRILDLGTGTGAIALALASERRDCAVIAVDINADAVALARHNAKKLAIDN
VCFLQGSWFEPVSGRFALIASNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLEPGGWLMLEHGWQ
QANAVQERLKNSGFSAVMTCKDYGNNDRVTLGQWSV

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=236, Percent_Identity=33.0508474576271, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=63.2727272727273, Blast_Score=359, Evalue=1e-101,
Organism=Escherichia coli, GI87082085, Length=203, Percent_Identity=31.5270935960591, Blast_Score=90, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24582226, Length=208, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30165; Mature: 30165

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLAN
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHH
RREQGEPIAYLVGEREFWSLPLSVSSATLIPRPDTECLVEQALARLPSMPCRILDLGTGT
HHHCCCCEEEEECCCEEEECCEECCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GAIALALASERRDCAVIAVDINADAVALARHNAKKLAIDNVCFLQGSWFEPVSGRFALIA
CEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCEEEECCEEEECCCCCCCCCCCEEEEE
SNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLEPGGWLMLEHGWQ
CCCCEECCCCCCCCCCCEEECCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEECCCH
QANAVQERLKNSGFSAVMTCKDYGNNDRVTLGQWSV
HHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCC
>Mature Secondary Structure
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLAN
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHH
RREQGEPIAYLVGEREFWSLPLSVSSATLIPRPDTECLVEQALARLPSMPCRILDLGTGT
HHHCCCCEEEEECCCEEEECCEECCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GAIALALASERRDCAVIAVDINADAVALARHNAKKLAIDNVCFLQGSWFEPVSGRFALIA
CEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCEEEECCEEEECCCCCCCCCCCEEEEE
SNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLEPGGWLMLEHGWQ
CCCCEECCCCCCCCCCCEEECCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEECCCH
QANAVQERLKNSGFSAVMTCKDYGNNDRVTLGQWSV
HHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]