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The map label for this gene is prfA [H]

Identifier: 170024410

GI number: 170024410

Start: 2417719

End: 2418801

Strand: Reverse

Name: prfA [H]

Synonym: YPK_2179

Alternate gene names: 170024410

Gene position: 2418801-2417719 (Counterclockwise)

Preceding gene: 170024411

Following gene: 170024409

Centisome position: 51.58

GC content: 51.52

Gene sequence:

>1083_bases
ATGAAGTCTTCTATTGTTGCCAAACTGGAAGCGTTACAAGAGCGCCATGAAGAAGTTCTGGCATATCTTGGCGATGCCAG
CGTTATTGCCGATCAAGACCGTTTCCGCGCGCTATCACGTGAATATGCGCAATTGACGGATGTCACTCGCTGTTTTAAAG
AGTGGCGCAGCGCTCAGGATGATATCGAAGCCGCAGAAATGATGCTGGACGATCTCGAAATGCGTGAAATGGCACAGGAA
GAATTAAAAATTGCCAAAGCGCGGAGCGAAGAGCTCGAGCAGCAGCTACAAGTGTTGTTGTTGCCAAAAGATCCTGATGA
TGAACGTGACTGCTTCCTGGAAATCCGCGCGGGGACTGGCGGCGATGAAGCCGCTATTTTTGCCGGTGATATGTTCCGTA
TGTACAGCCGTTATGCCGAAACCCGCCGCTGGAAAGTTGAAATCATGAGCGCCAGCGAGGGTGAGCATGGCGGTTATAAA
GAAATTATTGCCAAAATATCTGGCGATGGCGTCTTCGGCCAGTTGAAGTTTGAATCCGGTGGTCACCGGGTTCAGCGTGT
ACCCGAAACCGAGTCTCAAGGCCGCATCCACACTTCCGCCTGTACCGTTGCTGTGATGCCCGCCATCCCAGAGGCTGAAC
TGCCAGAAATCAACGCCGGTGACTTGCGTATAGATACGTTCCGCTCGTCAGGTGCGGGTGGTCAGCATGTTAACACCACC
GATTCAGCCATCCGTATTACACATATTCCTACCGGTATCGTGGTGGAGTGTCAGGACGAGCGTTCGCAGCACAAAAACAA
AGCGAAGGCAATGTCAGTGTTAGGCGCGCGTATTCGTGCTGCTGAAATGCAAAAACGGCAATTGGCAGAGGCATCAGAGC
GCCGTAACTTGTTAGGGACCGGTGATCGTTCTGACCGTAACCGTACTTACAACTTCCCGCAGGGGCGCGTTACTGACCAT
CGTATCAACCTGACGCTGTATCGGCTTGATGAAGTGATGGAAGGTAAACTGGATATGCTCATCCAGCCCATTGTGCAGGA
ATATCAAGCTGACCAACTGTCTGCATTGTCAGAGCAAGACTAA

Upstream 100 bases:

>100_bases
CCGCCAGCGATGGCGATATGGAGCGGTTGCAATTATTACGCGACAGCCTTGGGCTGGATCAGCATTAGTTTCCTCTAATT
AATAGGGTTTAAAACCACGG

Downstream 100 bases:

>100_bases
TGAATTATCAGCATTGGCTGTCGTTGGCGGCAGCCCGTTTTACCCACAGTGACAGCCCTAAGCGTGATGCTGAAATCCTA
CTGAGTTTTGTGACAGGTAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQDDIEAAEMMLDDLEMREMAQE
ELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTGGDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYK
EIIAKISGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD

Sequences:

>Translated_360_residues
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQDDIEAAEMMLDDLEMREMAQE
ELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTGGDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYK
EIIAKISGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD
>Mature_360_residues
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQDDIEAAEMMLDDLEMREMAQE
ELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTGGDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYK
EIIAKISGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=47.0588235294118, Blast_Score=264, Evalue=9e-71,
Organism=Homo sapiens, GI34577120, Length=332, Percent_Identity=40.6626506024096, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=47.3684210526316, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=85.5555555555556, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=39.0532544378698, Blast_Score=215, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=343, Percent_Identity=31.7784256559767, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=43.1309904153355, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19921226, Length=320, Percent_Identity=37.5, Blast_Score=201, Evalue=7e-52,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40564; Mature: 40564

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQD
CCCHHHHHHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DIEAAEMMLDDLEMREMAQEELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCC
GDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYKEIIAKISGDGVFGQLKFESG
CCCCEEEHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
CCHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGT
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD
CCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQD
CCCHHHHHHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DIEAAEMMLDDLEMREMAQEELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCC
GDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYKEIIAKISGDGVFGQLKFESG
CCCCEEEHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
CCHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGT
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD
CCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA