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The map label for this gene is msbB1 [H]

Identifier: 170024372

GI number: 170024372

Start: 2368925

End: 2369887

Strand: Reverse

Name: msbB1 [H]

Synonym: YPK_2138

Alternate gene names: 170024372

Gene position: 2369887-2368925 (Counterclockwise)

Preceding gene: 170024373

Following gene: 170024368

Centisome position: 50.54

GC content: 46.94

Gene sequence:

>963_bases
ATGCACAAAGAAAAAAATGACGCAACTGGTTTTATTCCGGTATTTCAGAAAACCTTCCTCCATCCGCGTTTCTGGGGGGT
TTGGTTAGGTGCTGGAGCGATTGCGGCGCTCGCTTATATTCCACCCAAATTCCGTGATCCTTTATTAGCTGGCATAGGGC
GTCTCGCTGGGAAATTTGCTAAAAGCGCCCGTCGTCGCGCTCGTATTAATCTTCTTTATTGTATGCCTGAGTTACCCGAG
TCTGAACGCGAACACATTATTGACCAGATGTTTGCTACCGCAGCTCAACCGTTAATGATGATGGCAGAACTTTGTTTTCG
TGATCCGAAAAAAGTGCTGACTCGCGTGCACTGGCATGGACAGGAAATACTGGATGAGCTGCAACAACAAGAGCGTAATG
TTATTTTGTTGGTCCCCCATGCCTGGTCCATTGATATTCCGGCTATGCTGCTAGCGGAGCAGGGCAAGCCAGTTGCAGGT
ATGTTCCATCATCAACGTAATCCATTGGTGGACTATCTATGGAACAGTGCGCGTTTACATTTCGGTGGTCGGATCCATGC
CCGCGAAAGTGGCATCAAACCTTTTATCAGTTCAGTACGCCAAGGATTCTGGGGTTACTACTTACCTGATGAAGATTATG
GTCCTGAGCAAAGTGAATTTGTTGATTTCTTCGCGACCTATAAGGCGACATTACCGGCAATAGGCCGTTTAATGAAAGTC
TGCCGTGCGGCCATTGTACCGATGTTCCCTGTTTATAATTATAGAGAGCATCGTCTTGATATCTATATCCGTCCGCCAAT
GGATGATTTGGCGGATGCTGATGACGCCTATATTGCCCGCCGGATGAATGAGGAAGTGGAGTTGTTGGTTAAGCCGAATC
CAGAGCAATATACATGGATCTTAAAGCTGTTAAAAACCCGGAAAGAGGGTGAAACCGAGCCCTATGTGCGCAAAGATCTT
TAG

Upstream 100 bases:

>100_bases
ACGTTGTCGCCTGGAAAGATGAAGGTTGCCAAAAAGAGTGTCTGTATAACGTGTCTTCTGTAATGATGATTAGCCCACCG
CCAATTCTAAGAGTTTCCCC

Downstream 100 bases:

>100_bases
GGTGTGATAACCGTTCAAGAGGGCGGTTAAATACAGCACCATTCAATGTAAAACGAGTAAATACAAACACGGCGAAATAT
AAAACAGTTAAAGGTAAAAG

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MHKEKNDATGFIPVFQKTFLHPRFWGVWLGAGAIAALAYIPPKFRDPLLAGIGRLAGKFAKSARRRARINLLYCMPELPE
SEREHIIDQMFATAAQPLMMMAELCFRDPKKVLTRVHWHGQEILDELQQQERNVILLVPHAWSIDIPAMLLAEQGKPVAG
MFHHQRNPLVDYLWNSARLHFGGRIHARESGIKPFISSVRQGFWGYYLPDEDYGPEQSEFVDFFATYKATLPAIGRLMKV
CRAAIVPMFPVYNYREHRLDIYIRPPMDDLADADDAYIARRMNEEVELLVKPNPEQYTWILKLLKTRKEGETEPYVRKDL

Sequences:

>Translated_320_residues
MHKEKNDATGFIPVFQKTFLHPRFWGVWLGAGAIAALAYIPPKFRDPLLAGIGRLAGKFAKSARRRARINLLYCMPELPE
SEREHIIDQMFATAAQPLMMMAELCFRDPKKVLTRVHWHGQEILDELQQQERNVILLVPHAWSIDIPAMLLAEQGKPVAG
MFHHQRNPLVDYLWNSARLHFGGRIHARESGIKPFISSVRQGFWGYYLPDEDYGPEQSEFVDFFATYKATLPAIGRLMKV
CRAAIVPMFPVYNYREHRLDIYIRPPMDDLADADDAYIARRMNEEVELLVKPNPEQYTWILKLLKTRKEGETEPYVRKDL
>Mature_320_residues
MHKEKNDATGFIPVFQKTFLHPRFWGVWLGAGAIAALAYIPPKFRDPLLAGIGRLAGKFAKSARRRARINLLYCMPELPE
SEREHIIDQMFATAAQPLMMMAELCFRDPKKVLTRVHWHGQEILDELQQQERNVILLVPHAWSIDIPAMLLAEQGKPVAG
MFHHQRNPLVDYLWNSARLHFGGRIHARESGIKPFISSVRQGFWGYYLPDEDYGPEQSEFVDFFATYKATLPAIGRLMKV
CRAAIVPMFPVYNYREHRLDIYIRPPMDDLADADDAYIARRMNEEVELLVKPNPEQYTWILKLLKTRKEGETEPYVRKDL

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=320, Percent_Identity=65, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1787292, Length=313, Percent_Identity=31.629392971246, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87082094, Length=305, Percent_Identity=27.8688524590164, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37089; Mature: 37089

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKEKNDATGFIPVFQKTFLHPRFWGVWLGAGAIAALAYIPPKFRDPLLAGIGRLAGKFA
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KSARRRARINLLYCMPELPESEREHIIDQMFATAAQPLMMMAELCFRDPKKVLTRVHWHG
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
QEILDELQQQERNVILLVPHAWSIDIPAMLLAEQGKPVAGMFHHQRNPLVDYLWNSARLH
HHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHCCCCCHHHHHHCCCEEE
FGGRIHARESGIKPFISSVRQGFWGYYLPDEDYGPEQSEFVDFFATYKATLPAIGRLMKV
CCCEEECCCCCCHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
CRAAIVPMFPVYNYREHRLDIYIRPPMDDLADADDAYIARRMNEEVELLVKPNPEQYTWI
HHHHHHHHCCCCCCCCCEEEEEECCCCHHHCCCCHHHHHHHCCCCEEEEECCCCHHHHHH
LKLLKTRKEGETEPYVRKDL
HHHHHHHCCCCCCCCHHCCC
>Mature Secondary Structure
MHKEKNDATGFIPVFQKTFLHPRFWGVWLGAGAIAALAYIPPKFRDPLLAGIGRLAGKFA
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KSARRRARINLLYCMPELPESEREHIIDQMFATAAQPLMMMAELCFRDPKKVLTRVHWHG
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
QEILDELQQQERNVILLVPHAWSIDIPAMLLAEQGKPVAGMFHHQRNPLVDYLWNSARLH
HHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHCCCCCHHHHHHCCCEEE
FGGRIHARESGIKPFISSVRQGFWGYYLPDEDYGPEQSEFVDFFATYKATLPAIGRLMKV
CCCEEECCCCCCHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
CRAAIVPMFPVYNYREHRLDIYIRPPMDDLADADDAYIARRMNEEVELLVKPNPEQYTWI
HHHHHHHHCCCCCCCCCEEEEEECCCCHHHCCCCHHHHHHHCCCCEEEEECCCCHHHHHH
LKLLKTRKEGETEPYVRKDL
HHHHHHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]