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The map label for this gene is rfbA [H]

Identifier: 169833959

GI number: 169833959

Start: 416408

End: 417277

Strand: Direct

Name: rfbA [H]

Synonym: SPH_0466

Alternate gene names: 169833959

Gene position: 416408-417277 (Clockwise)

Preceding gene: 169834375

Following gene: 169833448

Centisome position: 18.54

GC content: 43.45

Gene sequence:

>870_bases
ATGAAAGGTATTATTCTTGCAGGCGGCTCAGGTACCCGCCTGTACCCACTTACTCGGGCTGCGTCAAAACAGCTGATGCC
GGTTTATGATAAACCTATGATTTATTATCCGTTGTCGACATTAATGTTGGCTGGAATTAAAGATATTTTGATTATCTCAA
CTCCTCAAGATTTGCCCCGTTTTAAGGACTTGCTCTTGGATGGTTCCGAATTTGGGATCAAGCTTTCCTATGCGGAACAA
CCTAGTCCCGATGGACTTGCTCAGGCTTTTCTTATCGGTGAAGAATTTATCGGTGACGATAGCGTTGCCTTGATTTTAGG
CGACAATATCTACCATGGTCCTGGTCTGAGCACAATGCTTCAAAAAGCAGCCAAGAAAGAGAAAGGTGCGACTGTTTTTG
GCTACCAAGTGAAGGATCCAGAGCGTTTTGGTGTGGTTGAGTTTGATACAGACATGAATGCTATTTCTATAGAAGAAAAA
CCGGAGTATCCTCGCTCCAACTATGCAGTGACAGGACTGTATTTCTATGATAATGATGTTGTGGAGATTGCTAAACAGAT
CAAACCTAGTGCTCGTGGCGAGTTAGAAATTACAGACGTTAACAAGGCTTACCTAAATCGTGGTGACCTTTCTGTTGAGC
TGATGGGGCGTGGTTTTGCCTGGTTGGATACGGGAACCCATGAAAGCTTGCTAGAAGCTTCTCAGTATATTGAAACGGTT
CAGCGTATGCAGAACGTTCAAGTGGCAAATCTAGAAGAAATTTCCTATCGTATGGGCTACATTAGTCGTGAAGACGTGCT
GGAATTGGCTCAGCCTCTTAAGAAAAATGAATACGGACAATATTTGCTCCGTTTGATTGGAGAAGCATAG

Upstream 100 bases:

>100_bases
ATTTATGATTGAGATAGACTCATAACTTAATATTTTTATCCTAAGAAAATTCGTTCATATTCAATATGAATGAAAAACAA
CGAAGAAAGGTTATTTCATT

Downstream 100 bases:

>100_bases
ATGTCAGATAATTTTTTTGGAAAGACACTTGTGGTACGCAAGATTGATGCTATACCAGGACTGCTAGAGTTTGATATTCC
CGTTCATGGAGACAATCGTG

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLAGIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQ
PSPDGLAQAFLIGEEFIGDDSVALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRGFAWLDTGTHESLLEASQYIETV
QRMQNVQVANLEEISYRMGYISREDVLELAQPLKKNEYGQYLLRLIGEA

Sequences:

>Translated_289_residues
MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLAGIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQ
PSPDGLAQAFLIGEEFIGDDSVALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRGFAWLDTGTHESLLEASQYIETV
QRMQNVQVANLEEISYRMGYISREDVLELAQPLKKNEYGQYLLRLIGEA
>Mature_289_residues
MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLAGIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQ
PSPDGLAQAFLIGEEFIGDDSVALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRGFAWLDTGTHESLLEASQYIETV
QRMQNVQVANLEEISYRMGYISREDVLELAQPLKKNEYGQYLLRLIGEA

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=239, Percent_Identity=25.5230125523013, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI11761619, Length=253, Percent_Identity=24.5059288537549, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788351, Length=288, Percent_Identity=67.3611111111111, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1790224, Length=286, Percent_Identity=65.7342657342657, Blast_Score=396, Evalue=1e-112,
Organism=Escherichia coli, GI1787488, Length=236, Percent_Identity=26.6949152542373, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=237, Percent_Identity=24.8945147679325, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=254, Percent_Identity=27.1653543307087, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21355443, Length=249, Percent_Identity=23.6947791164659, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24644084, Length=249, Percent_Identity=23.6947791164659, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32228; Mature: 32228

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLAGIKDILIISTPQDLPR
CCCEEEECCCCCEEEEEHHHHCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCHHHH
FKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSVALILGDNIYHGPGLSTML
HHHHHCCCCHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEECCCCHHHHH
QKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYPRSNYAVTGLYFYDNDV
HHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCHH
VEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRGFAWLDTGTHESLLEASQYIETV
HHHHHHCCCCCCCCEEEEECCHHHHCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHH
QRMQNVQVANLEEISYRMGYISREDVLELAQPLKKNEYGQYLLRLIGEA
HHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLAGIKDILIISTPQDLPR
CCCEEEECCCCCEEEEEHHHHCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCHHHH
FKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSVALILGDNIYHGPGLSTML
HHHHHCCCCHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEECCCCHHHHH
QKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYPRSNYAVTGLYFYDNDV
HHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCHH
VEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRGFAWLDTGTHESLLEASQYIETV
HHHHHHCCCCCCCCEEEEECCHHHHCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHH
QRMQNVQVANLEEISYRMGYISREDVLELAQPLKKNEYGQYLLRLIGEA
HHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]