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The map label for this gene is mtlF [H]

Identifier: 169832843

GI number: 169832843

Start: 458464

End: 458901

Strand: Direct

Name: mtlF [H]

Synonym: SPH_0506

Alternate gene names: 169832843

Gene position: 458464-458901 (Clockwise)

Preceding gene: 169834153

Following gene: 169833317

Centisome position: 20.42

GC content: 36.07

Gene sequence:

>438_bases
ATGAAACTTGAAAAACATTTGATTAAGCTTAATAAACAATTTTCTAACAAGGAGGAAGCTATTTGTTATTGTGGGCAAGT
TCTTTATGAGGGTGGATATGTTAATGAAGACTATATTGAAGCCATGATTGAGCGAGATAAAGAGCTATCTGTTTACATGG
GTAACTTTATCGCCATACCGCATGGAACAGATGCAGCAAAAAATGATGTCCTTAAGTCTGGTATTACAGTCGTTCAAGTC
CCTAGAGGGGTTGATTTTGGGAATGTATCTAACCCTCAAGTGGCAACGGTTCTTTTTGGTATTGCTGGTATTGGTAATGA
ACACTTAGAAATTATTCAGAAAATTTCTATCTTCTGTGCAGATGTGGATAATGTTCTTAAACTAGCAGATGCTCAGTCAA
AAGAGGAAGTATTGCGCTTATTTGATGCTGTTGAATAA

Upstream 100 bases:

>100_bases
CATCTTTATACAGAGATTTACAAGACGGGAAATCAATCCATTATTTATCAGATGCTAAATACTATTTTTAACGAAAAAAT
TAAGAAATTGGAGAACTAAT

Downstream 100 bases:

>100_bases
TTGAATTTCGTCATTTGTCATCTAGTATATATGTCCCTCAAATAGGAAAAGGAGAAATTGAATGAAACATTCTGTTCATT
TTGGTGCCGGTAATATCGGT

Product: mannitol-specific phosphotransferase enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIERDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQV
PRGVDFGNVSNPQVATVLFGIAGIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE

Sequences:

>Translated_145_residues
MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIERDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQV
PRGVDFGNVSNPQVATVLFGIAGIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE
>Mature_145_residues
MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIERDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQV
PRGVDFGNVSNPQVATVLFGIAGIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=140, Percent_Identity=40, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI1788494, Length=134, Percent_Identity=36.5671641791045, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16063; Mature: 16063

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIERDKELSVYMGNFIAIP
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCEEECC
HGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIAGIGNEHLEIIQKISIFCA
CCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DVDNVLKLADAQSKEEVLRLFDAVE
CHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIERDKELSVYMGNFIAIP
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCEEECC
HGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIAGIGNEHLEIIQKISIFCA
CCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DVDNVLKLADAQSKEEVLRLFDAVE
CHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1322373; 12397186 [H]