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The map label for this gene is gltX

Identifier: 169824111

GI number: 169824111

Start: 470477

End: 471946

Strand: Direct

Name: gltX

Synonym: FMG_0414

Alternate gene names: 169824111

Gene position: 470477-471946 (Clockwise)

Preceding gene: 169824110

Following gene: 169824112

Centisome position: 26.17

GC content: 33.27

Gene sequence:

>1470_bases
ATGAGTGAAGTAAGAGTAAGATTTGCTCCAAGTCCAACAGGATTTTTACACATTGGTGGACTTAGAACAGCTTTATATAA
TTATTTGTATGCAAAAAGAAATAACGGTAAATTTCTTTTGAGAATTGAAGATACTGATAGAACAAGATATGTTGAAGGTG
CTATCGAAAATTTGTTGGAACAATTAAAATGGGCAGGACTTGATCCAGATGAAGGAGTTGTCCTTGATGATGAAGGAAAC
GTAACTGAAGTTGGAGAATGTGGTCCTTACATTCAATCTGACAGAGTAAAACAAGGATTATACCAAAAATATATCGACGA
ATTAATCGAAAAAGGTTATGCATATTATTGTTTCTGTTCAAAAGAAAGATTGGATCAAGTAAAAGCACAACAAAAAGCAG
ATGGTTTGATGCCAAAATACGACGGACTTTGCAGAGGAATTAGCATTGAGGATGCTAAAAAGAGAATTGCAAACGGCGAG
GAATATGTTATTAGATTGAAACTTCCTGAAAACAAAGAAATCACATTTAACGATGCGATAAAAGGTAAGATTACTTTCAA
CACAAATGATATGGACGATCAAGTTTTGATAAAATCTGACGGATTTCCAACTTATCACTTTGCAGTTGTAGTTGACGATC
ATTTGATGGGAATTACTCACATTGTTCGTGGTGATGAATGGATTTCTTCAACAGCAAAACACGTGTATTTGTATCAATGT
TTCGGATGGGATGTCCCAGAATTCGTTCACTTACCAGTTGTTTTGAACAAATCAGGTAAGAAATTATCCAAGAGAAATGA
TGACGTTGCAGTTAAAGATTTCAGAAAAAAAGGATATTTGCCAGAAGCTATTGACAATTACCTAGCTTTAGTAGGTTGGT
CAAGTGAAGATAACCAAGAAATTATGTCGATGGAAGAATTAAAACACAAATTTGATTTCAACAGAGTTTCAAAGTCGGGC
GGAGTTTTCGACACAGAAAAATTAAACTGGATTAACAGACATTACATTAAAGAAATAGAAAATGAAAAATTAGCATCAAT
GTTAAAACCATACTTGGTTGAAGATGGTGTGATTTCTGAAGATTATCCAGAATACAAATTAATAGAGATTGCTTCATTAT
TCAAAGAAGAATTGGATTACATGGCAGAAATAACTGAAAAAGTTGAATTCTTGTTCAAAGATTACGAAATGGATGACGAT
GCTAAGGAATTCTTAAACTACGAAAAATTAGACGAATTAATGAACGCACTAAAAGAAGAAATCGAATCTGTTGATGAAAT
AGAAAAAGAATTTGCATCAGGCGTTATGAAAAAAGTTCAAAAGAAAACTGGAATCAAGGGCAAGGATTTGTGGATGACTA
CAAGAGCTGTAGTTACTGGTAATGTTCACGGACCAGACTTGGATTCTATTATGGTAGTTTTAGGAAAACAAGAAGTTTTA
GATAGGATTAACAAAGCTTTAAATAGATAG

Upstream 100 bases:

>100_bases
AATTTGGCGAAATAATTGCCAGATTTGTATTAATAAATGTGAGATTTGGCGATTTGTGATATAATAAAATAAGTTAAATT
TATTTTTTATGGGGGATATT

Downstream 100 bases:

>100_bases
GAGAAGATATGAAGTTATACAATACACTGACACGAAAAAAGGAAGAATTCAAACCAATTAAAGAAAAAAATGTCGGTATA
TACGTATGTGGCCCTACTGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSEVRVRFAPSPTGFLHIGGLRTALYNYLYAKRNNGKFLLRIEDTDRTRYVEGAIENLLEQLKWAGLDPDEGVVLDDEGN
VTEVGECGPYIQSDRVKQGLYQKYIDELIEKGYAYYCFCSKERLDQVKAQQKADGLMPKYDGLCRGISIEDAKKRIANGE
EYVIRLKLPENKEITFNDAIKGKITFNTNDMDDQVLIKSDGFPTYHFAVVVDDHLMGITHIVRGDEWISSTAKHVYLYQC
FGWDVPEFVHLPVVLNKSGKKLSKRNDDVAVKDFRKKGYLPEAIDNYLALVGWSSEDNQEIMSMEELKHKFDFNRVSKSG
GVFDTEKLNWINRHYIKEIENEKLASMLKPYLVEDGVISEDYPEYKLIEIASLFKEELDYMAEITEKVEFLFKDYEMDDD
AKEFLNYEKLDELMNALKEEIESVDEIEKEFASGVMKKVQKKTGIKGKDLWMTTRAVVTGNVHGPDLDSIMVVLGKQEVL
DRINKALNR

Sequences:

>Translated_489_residues
MSEVRVRFAPSPTGFLHIGGLRTALYNYLYAKRNNGKFLLRIEDTDRTRYVEGAIENLLEQLKWAGLDPDEGVVLDDEGN
VTEVGECGPYIQSDRVKQGLYQKYIDELIEKGYAYYCFCSKERLDQVKAQQKADGLMPKYDGLCRGISIEDAKKRIANGE
EYVIRLKLPENKEITFNDAIKGKITFNTNDMDDQVLIKSDGFPTYHFAVVVDDHLMGITHIVRGDEWISSTAKHVYLYQC
FGWDVPEFVHLPVVLNKSGKKLSKRNDDVAVKDFRKKGYLPEAIDNYLALVGWSSEDNQEIMSMEELKHKFDFNRVSKSG
GVFDTEKLNWINRHYIKEIENEKLASMLKPYLVEDGVISEDYPEYKLIEIASLFKEELDYMAEITEKVEFLFKDYEMDDD
AKEFLNYEKLDELMNALKEEIESVDEIEKEFASGVMKKVQKKTGIKGKDLWMTTRAVVTGNVHGPDLDSIMVVLGKQEVL
DRINKALNR
>Mature_488_residues
SEVRVRFAPSPTGFLHIGGLRTALYNYLYAKRNNGKFLLRIEDTDRTRYVEGAIENLLEQLKWAGLDPDEGVVLDDEGNV
TEVGECGPYIQSDRVKQGLYQKYIDELIEKGYAYYCFCSKERLDQVKAQQKADGLMPKYDGLCRGISIEDAKKRIANGEE
YVIRLKLPENKEITFNDAIKGKITFNTNDMDDQVLIKSDGFPTYHFAVVVDDHLMGITHIVRGDEWISSTAKHVYLYQCF
GWDVPEFVHLPVVLNKSGKKLSKRNDDVAVKDFRKKGYLPEAIDNYLALVGWSSEDNQEIMSMEELKHKFDFNRVSKSGG
VFDTEKLNWINRHYIKEIENEKLASMLKPYLVEDGVISEDYPEYKLIEIASLFKEELDYMAEITEKVEFLFKDYEMDDDA
KEFLNYEKLDELMNALKEEIESVDEIEKEFASGVMKKVQKKTGIKGKDLWMTTRAVVTGNVHGPDLDSIMVVLGKQEVLD
RINKALNR

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=503, Percent_Identity=37.1769383697813, Blast_Score=320, Evalue=3e-87,
Organism=Homo sapiens, GI4826960, Length=308, Percent_Identity=26.6233766233766, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI62241042, Length=268, Percent_Identity=24.2537313432836, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788743, Length=487, Percent_Identity=35.3182751540041, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI226510925, Length=304, Percent_Identity=34.8684210526316, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=35.8762886597938, Blast_Score=285, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6324540, Length=511, Percent_Identity=35.4207436399217, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6321192, Length=310, Percent_Identity=23.8709677419355, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=507, Percent_Identity=39.4477317554241, Blast_Score=357, Evalue=1e-98,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_FINM2 (B0S0W2)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691722.1
- ProteinModelPortal:   B0S0W2
- SMR:   B0S0W2
- GeneID:   6019878
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0414
- HOGENOM:   HBG628189
- OMA:   YRCFTSE
- ProtClustDB:   PRK01406
- BioCyc:   FMAG334413:FMG_0414-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56325; Mature: 56194

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVRVRFAPSPTGFLHIGGLRTALYNYLYAKRNNGKFLLRIEDTDRTRYVEGAIENLLE
CCCCEEEECCCCCCEEEECHHHHHHHHHHHEEECCCEEEEEEECCCCHHHHHHHHHHHHH
QLKWAGLDPDEGVVLDDEGNVTEVGECGPYIQSDRVKQGLYQKYIDELIEKGYAYYCFCS
HHHHCCCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
KERLDQVKAQQKADGLMPKYDGLCRGISIEDAKKRIANGEEYVIRLKLPENKEITFNDAI
HHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCEEECCCE
KGKITFNTNDMDDQVLIKSDGFPTYHFAVVVDDHLMGITHIVRGDEWISSTAKHVYLYQC
EEEEEECCCCCCCEEEEECCCCCEEEEEEEECCHHHHHHHHHCCCHHHHCCCCEEEEEEE
FGWDVPEFVHLPVVLNKSGKKLSKRNDDVAVKDFRKKGYLPEAIDNYLALVGWSSEDNQE
ECCCCHHHEEECEEECCCCCHHHCCCCCHHHHHHHHCCCCHHHHHCCEEEEECCCCCCHH
IMSMEELKHKFDFNRVSKSGGVFDTEKLNWINRHYIKEIENEKLASMLKPYLVEDGVISE
HHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DYPEYKLIEIASLFKEELDYMAEITEKVEFLFKDYEMDDDAKEFLNYEKLDELMNALKEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHH
IESVDEIEKEFASGVMKKVQKKTGIKGKDLWMTTRAVVTGNVHGPDLDSIMVVLGKQEVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCHHHHH
DRINKALNR
HHHHHHHCC
>Mature Secondary Structure 
SEVRVRFAPSPTGFLHIGGLRTALYNYLYAKRNNGKFLLRIEDTDRTRYVEGAIENLLE
CCCEEEECCCCCCEEEECHHHHHHHHHHHEEECCCEEEEEEECCCCHHHHHHHHHHHHH
QLKWAGLDPDEGVVLDDEGNVTEVGECGPYIQSDRVKQGLYQKYIDELIEKGYAYYCFCS
HHHHCCCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
KERLDQVKAQQKADGLMPKYDGLCRGISIEDAKKRIANGEEYVIRLKLPENKEITFNDAI
HHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCEEECCCE
KGKITFNTNDMDDQVLIKSDGFPTYHFAVVVDDHLMGITHIVRGDEWISSTAKHVYLYQC
EEEEEECCCCCCCEEEEECCCCCEEEEEEEECCHHHHHHHHHCCCHHHHCCCCEEEEEEE
FGWDVPEFVHLPVVLNKSGKKLSKRNDDVAVKDFRKKGYLPEAIDNYLALVGWSSEDNQE
ECCCCHHHEEECEEECCCCCHHHCCCCCHHHHHHHHCCCCHHHHHCCEEEEECCCCCCHH
IMSMEELKHKFDFNRVSKSGGVFDTEKLNWINRHYIKEIENEKLASMLKPYLVEDGVISE
HHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DYPEYKLIEIASLFKEELDYMAEITEKVEFLFKDYEMDDDAKEFLNYEKLDELMNALKEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHH
IESVDEIEKEFASGVMKKVQKKTGIKGKDLWMTTRAVVTGNVHGPDLDSIMVVLGKQEVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCHHHHH
DRINKALNR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA