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The map label for this gene is gcl [H]

Identifier: 16763897

GI number: 16763897

Start: 577467

End: 579248

Strand: Direct

Name: gcl [H]

Synonym: STM0517

Alternate gene names: 16763897

Gene position: 577467-579248 (Clockwise)

Preceding gene: 16763896

Following gene: 16763898

Centisome position: 11.89

GC content: 54.32

Gene sequence:

>1782_bases
ATGGCAAAAATGAGAGCCGTGGATGCGGCAATGTATGTGCTGGAAAAAGAAGGTATCACCACAGCATTCGGCGTTCCTGG
GGCTGCAATCAACCCCTTCTATTCTGCAATGCGTAAGCATGGAGGCATTCGCCATATACTGGCGCGTCATGTGGAAGGCG
CTTCGCATATGGCTGAGGGCTACACACGTGCGGCGGCAGGTAATATTGGGGTCTGCCTGGGCACTTCCGGCCCGGCGGGA
ACCGACATGATCACCGCGCTCTATTCCGCTTCTGCCGACTCCATCCCGATTTTATGCATTACCGGCCAGGCGCCACGAGC
GCGTCTGCACAAGGAAGATTTCCAGGCAGTCGATATTGAAGCGATTGCAAAACCAGTCAGCAAGATGGCAGTGACGGTTC
GTGAGGCGGCGCTGGTGCCGCGCGTATTACAACAGGCTTTTCATCTGATGCGCTCAGGCCGCCCAGGTCCTGTGCTGGTT
GATTTACCGTTTGATGTCCAGGTTGCGGAGATCGAATTCGATCCGGATATGTATGAGCCGTTGCCGGTATATAAGCCAGC
CGCCAGCCGCGTACAGATTGAAAAGGCGCTGGAGATGCTGATTCAGTCCGAGCGTCCGGTAATTGTCGCGGGCGGCGGGG
TTATTAACGCCGACGCCGCGCCACTGCTGCAGCAGTTTGCCGAACTGACCAACGTACCGGTGATCCCTACCCTGATGGGC
TGGGGCTGTATCCCGGACGATCACCCCTTAATGGCGGGGATGGTCGGCCTGCAAACGGCGCACCGCTACGGCAACGCCAC
TCTGCTGGCATCCGATATGGTTTTTGGCATCGGTAACCGCTTCGCCAACCGTCATACGGGGTCCGTTGAGAAATATACTC
AGGGGCGAAAAATCATCCATATCGATATTGAACCGACGCAAATTGGTCGCGTGTTGTGCCCGGATCTAGGCATCGTTTCG
GACGCCAAAGCGGCGCTAACGTTGCTGATCGATGTCGCGCAGGAGATGCAAAAGGCGGGGCGTTTGCCATGCCGGAAGAC
CTGGGTGGACGAATGCCAGCAGCGTAAACGGACTTTGCTGCGTAAAACCCACTTTGACAACGTGCCAGTGAAGCCGCAGC
GCGTCTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTGTGCTATGTCACCACCATTGGATTGTCGCAAATCGCGGCA
GCGCAGATGCTGCATGTGTTTAAAGACAGACACTGGATCAACTGCGGCCAGGCGGGGCCTCTTGGCTGGACAATCCCGGC
GGCATTAGGGGTTTGTGCTGCCGATCCGCAACGCAATGTGGTGGCGATTTCCGGCGATTTCGACTTCCAGTTCCTGATTG
AAGAGTTAGCCGTCGGCGCGCAGTTTAACATTCCGTATATCCATGTGCTGGTTAACAACGCTTATCTTGGTCTGATTCGC
CAGTCGCAACGCGCATTTGACATGGACTACTGCGTACAGCTTGCCTTTGAAAATATCAACTCCAGCGAAGTGAATGGCTA
TGGCGTTGACCATGTAAAAGTGGCGGAAGGGTTAGGCTGTAAAGCAATTCGTGTCTTTAAACCGGAAGATATTGCGCCTG
CATTTGAACAAGCCAAAGCGCTAATGGCGCAATATCGCGTTCCGGTGGTGGTGGAAGTCATTCTGGAACGCGTAACCAAT
ATTTCCATGGGCAGCGAACTGGATAACGTAACCGAGTTTGAAGAGGTTGCGGATAGTGCGAAAGATGCGCCAACAGAAAC
CTGTTTTATGAAATATGAGTAA

Upstream 100 bases:

>100_bases
ACCCCCTGTAGCCTGAGCCGCATCCCGCTCGCTGGAGCGGGACTTTTTTAAAATTTTTAAAAGTTGGAAACTTTTTCCAA
TAAAAAGAGGTAGCAATAAA

Downstream 100 bases:

>100_bases
GGGAGGTAATCGATGTTACGTTTCTCTGCCAATCTTTCTATGCTGTTTACGGAATATGATTTCCTGGAACGCTTTGATAA
AGCGGCGTTGTCGGGATTTC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRAAAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
DLPFDVQVAEIEFDPDMYEPLPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELTNVPVIPTLMG
WGCIPDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIHIDIEPTQIGRVLCPDLGIVS
DAKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTN
ISMGSELDNVTEFEEVADSAKDAPTETCFMKYE

Sequences:

>Translated_593_residues
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRAAAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
DLPFDVQVAEIEFDPDMYEPLPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELTNVPVIPTLMG
WGCIPDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIHIDIEPTQIGRVLCPDLGIVS
DAKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTN
ISMGSELDNVTEFEEVADSAKDAPTETCFMKYE
>Mature_592_residues
AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRAAAGNIGVCLGTSGPAGT
DMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVD
LPFDVQVAEIEFDPDMYEPLPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELTNVPVIPTLMGW
GCIPDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIHIDIEPTQIGRVLCPDLGIVSD
AKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAA
QMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIRQ
SQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNI
SMGSELDNVTEFEEVADSAKDAPTETCFMKYE

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=572, Percent_Identity=23.0769230769231, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI21361361, Length=601, Percent_Identity=23.9600665557404, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1786717, Length=593, Percent_Identity=96.4586846543002, Blast_Score=1191, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=33.8028169014084, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1790104, Length=476, Percent_Identity=34.0336134453782, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=26.0948905109489, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788716, Length=486, Percent_Identity=26.7489711934156, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=503, Percent_Identity=25.844930417495, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=503, Percent_Identity=25.844930417495, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17542570, Length=543, Percent_Identity=24.1252302025783, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=30.6397306397306, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6320816, Length=563, Percent_Identity=23.6234458259325, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=497, Percent_Identity=22.1327967806841, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=501, Percent_Identity=21.7564870259481, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922626, Length=489, Percent_Identity=24.5398773006135, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64756; Mature: 64624

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
YTRAAAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELTNVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCCHHHHC
WGCIPDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIH
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLL
EECCHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
LMAQYRVPVVVEVILERVTNISMGSELDNVTEFEEVADSAKDAPTETCFMKYE
HHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECC
>Mature Secondary Structure 
AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
YTRAAAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELTNVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCCHHHHC
WGCIPDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIH
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLL
EECCHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
LMAQYRVPVVVEVILERVTNISMGSELDNVTEFEEVADSAKDAPTETCFMKYE
HHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]