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The map label for this gene is epsD [H]

Identifier: 167626079

GI number: 167626079

Start: 5073520

End: 5075682

Strand: Reverse

Name: epsD [H]

Synonym: Shal_4174

Alternate gene names: 167626079

Gene position: 5075682-5073520 (Counterclockwise)

Preceding gene: 167626080

Following gene: 167626078

Centisome position: 97.11

GC content: 47.2

Gene sequence:

>2163_bases
ATGAATAATAAGCGGATTAGACGCAGGCTAATCGCCGGTATGGTTATGGGGGCATCTTTATTAGCTCCTCAGCTTGCTTG
GTCTGAGCAATATGCGGCTAACTTTAAAGGCACCGATATTCAAGAGTTTATCAACATTGTTGGTAAAAACTTGGAACGGA
CTATTATTGTTGATCCAACCGTTCGGGGTAAAATTAACGTCCGTAGTTATGATCTTCTTAACGACGAACAGTACTACCAA
TTCTTCCTCAATGTTTTACAAGTCTACGGTTATGCTGTTGTCGAAATGGACAACAACATCATCAAGGTGATCAAGGATAA
AGATGCTAAAACCGCATCAATTCGCGTCGCCGATGATAGTGCCCCAGGACTCGGTGATGAGATGGTCACCCGTATCGTCG
CGCTTTATAACACCGAGGCCAAGCAACTTGCCCCTTTATTGCGCCAGCTAAATGATAACGCTGGCGGTGGTAACGTTGTT
AATTATGACCCTTCAAACGTATTGATGATCTCTGGCCGTGCGGCCGTAGTGAATAAGCTAGTTGAAATCGTACGCCGCGT
AGATAAGCAAGGTGATACTGCCGTTGAGGTGGTAAGGCTTGAGTACGCTTCTGCGGGTGAAATTGTACGTATCATCGATA
CCCTTTATCGCTCTACTGCGAATCAGGCACAGATGCCAGGTCAGGCGCCAAAAGTGGTGGCCGATGAGCGTATTAACGCG
GTAGTTGTCAGTGGCGATGAGAAGAGCCGTGAGCGTGTGGTTAAACTCATTAAGAAGCTCGATGCAGAGCAGGCAACCAC
AGGTAACACCAAGGTGCGCTACCTGCGTTATGCCAAGGCTGAAGATTTAGTTGAAGTATTAACCGGTTTTGCTGAGCAGC
TTGTTGATGACAAGGGCGCGCCACAAGGTGGCGGCAGCAAACGTCGTAACGAAATTAATATTATGGCTCACACCGATACC
AATGCCTTGGTGATTAGCGCCGAGCCTGATCAGATGCGCACCATTGAGAGTGTCATTAATCAATTGGATATACGCCGTGC
ACAGGTATTGGTTGAAGCGATTATCGTCGAAGTCGCTGAAGGTGATGATGTTGGCTTTGGTGTGCAGTGGGCCACAGAAG
CTGGCGGTGGTACCCAGTTTAATAATCTAGGCCCTACCATTGGTGAAATTGGTGCTGGTATTTGGCAAGCACAAGGTGAA
GAAGGTTCTACCGTTTGTAACGACGGCACTTGTACCGAGAACCCTGATACCCGTGGTGACATTACGCTATTGGCACAGGC
GCTAGGCAAGGTAAACGGTATGGCATGGGGCGTGGCTATGGGTGACTTTGGTGCCTTGATCCAAGCGGTTTCCAGTGATA
CCAAGTCAAACGTATTAGCAACGCCATCTATCACTACCTTAGATAACCAAGAGGCCTCGTTTATCGTCGGTGACGAAGTG
CCGATTTTGACCGGTAGCCAAAACTCCAGTAACGGTAACAGTAATCCATTCCAAACCGTTGAACGTAAAGAGGTCGGCGT
AAAGCTTAAAGTCGTCCCTCAGGTTAACGAAGGTAACGCCGTTAAGCTGACTATTGAGCAGGAAGTGTCAGGTATTAACG
GCAAAACGGGTGTCGACGTAACCTTCGCGACTCGCCGTTTAACCACCACGGTTATGGCCGACTCGGGTCAGATCGTGGTG
CTAGGTGGTTTAATTAAAGAAGAGGTGCAAGAGTCGGTACAGAAGGTACCGTTCTTAGGCGATATCCCAATTATCGGTCA
TCTGTTTAAGTCGTCTTCGAGTGGTAAGAAAAAAACCAACTTGATGGTGTTTATCAAGCCGACGATTATTCGTGACGGTA
TCACCATGGAAGGCATTGCAGGGCGTAAGTATAACTACTTCCGCGCATTACAGCTTGAACAGCAAGAGCGCGGCGTTAAC
TTGATGCCAAATACCGATGTGCCAATCCTCGAAGAGTGGAATCAGGCTGATTATTTACCACCAGAGGTCAATGAAGTACT
TAACCGTTACAAAGAAGGTAAAGGCTTAGATACTAAGATGCGTCAAACCGATCCGACACTTAAGAGTATTTCAGACAGTA
AGAATACCGATGCTGCTGAAGTGGATAAGCAGTCGACAGAGTTAGAACATACTGGTACGGATAAAGATTCAAGCGATGAA
TGA

Upstream 100 bases:

>100_bases
GTTTAGTCTGCCAGAGTAATCGCAGCGGGAAATAACAATAGAATTTGCAGTAATACCAATTGAGTTTGCCGCAACGGAAG
GTTTTAGGGGAAGAATAGCA

Downstream 100 bases:

>100_bases
TAATCAAATTCCGGTCGATGAAGAGCTAGCGCAAGTATCGAGTGAGCTTGGGCTGGTGTCTGAGGAAGAAGCCGATCAAG
TCTTCCATTCGAGCAGCAAA

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLERTIIVDPTVRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDSAPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMISGRAAVVNKLVEIVRRVDKQGDTAVEVVRLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERINA
VVVSGDEKSRERVVKLIKKLDAEQATTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGAPQGGGSKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGIWQAQGE
EGSTVCNDGTCTENPDTRGDITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQVNEGNAVKLTIEQEVSGINGKTGVDVTFATRRLTTTVMADSGQIVV
LGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTNLMVFIKPTIIRDGITMEGIAGRKYNYFRALQLEQQERGVN
LMPNTDVPILEEWNQADYLPPEVNEVLNRYKEGKGLDTKMRQTDPTLKSISDSKNTDAAEVDKQSTELEHTGTDKDSSDE

Sequences:

>Translated_720_residues
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLERTIIVDPTVRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDSAPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMISGRAAVVNKLVEIVRRVDKQGDTAVEVVRLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERINA
VVVSGDEKSRERVVKLIKKLDAEQATTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGAPQGGGSKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGIWQAQGE
EGSTVCNDGTCTENPDTRGDITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQVNEGNAVKLTIEQEVSGINGKTGVDVTFATRRLTTTVMADSGQIVV
LGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTNLMVFIKPTIIRDGITMEGIAGRKYNYFRALQLEQQERGVN
LMPNTDVPILEEWNQADYLPPEVNEVLNRYKEGKGLDTKMRQTDPTLKSISDSKNTDAAEVDKQSTELEHTGTDKDSSDE
>Mature_720_residues
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLERTIIVDPTVRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDSAPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMISGRAAVVNKLVEIVRRVDKQGDTAVEVVRLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERINA
VVVSGDEKSRERVVKLIKKLDAEQATTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGAPQGGGSKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGIWQAQGE
EGSTVCNDGTCTENPDTRGDITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQVNEGNAVKLTIEQEVSGINGKTGVDVTFATRRLTTTVMADSGQIVV
LGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTNLMVFIKPTIIRDGITMEGIAGRKYNYFRALQLEQQERGVN
LMPNTDVPILEEWNQADYLPPEVNEVLNRYKEGKGLDTKMRQTDPTLKSISDSKNTDAAEVDKQSTELEHTGTDKDSSDE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=653, Percent_Identity=46.0949464012251, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1789793, Length=344, Percent_Identity=24.7093023255814, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 78393; Mature: 78393

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLERTIIVDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCC
VRGKINVRSYDLLNDEQYYQFFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDS
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCEEEEEEECCC
APGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMISGRAAVVNKL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHHHHH
VEIVRRVDKQGDTAVEVVRLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERINA
HHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHCCCCCCCCEECCCCCCE
VVVSGDEKSRERVVKLIKKLDAEQATTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGA
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCC
PQGGGSKRRNEINIMAHTDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAE
CCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGIWQAQGEEGSTVCNDGTCTENPDTRGD
CCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCEECCCCCCCEEECCCCCCCCCCCCCH
ITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
HHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCEEEECCCC
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQVNEGNAVKLTIEQEVSGINGKTGVDV
EEEECCCCCCCCCCCCHHHHHHHHCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCEE
TFATRRLTTTVMADSGQIVVLGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTN
EEEHHEEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
LMVFIKPTIIRDGITMEGIAGRKYNYFRALQLEQQERGVNLMPNTDVPILEEWNQADYLP
EEEEEEEEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHHCCCCCCCCC
PEVNEVLNRYKEGKGLDTKMRQTDPTLKSISDSKNTDAAEVDKQSTELEHTGTDKDSSDE
HHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCCCHHHHCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLERTIIVDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCC
VRGKINVRSYDLLNDEQYYQFFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDS
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCEEEEEEECCC
APGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMISGRAAVVNKL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHHHHH
VEIVRRVDKQGDTAVEVVRLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERINA
HHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHCCCCCCCCEECCCCCCE
VVVSGDEKSRERVVKLIKKLDAEQATTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGA
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCC
PQGGGSKRRNEINIMAHTDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAE
CCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGIWQAQGEEGSTVCNDGTCTENPDTRGD
CCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCEECCCCCCCEEECCCCCCCCCCCCCH
ITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
HHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCEEEECCCC
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQVNEGNAVKLTIEQEVSGINGKTGVDV
EEEECCCCCCCCCCCCHHHHHHHHCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCEE
TFATRRLTTTVMADSGQIVVLGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTN
EEEHHEEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
LMVFIKPTIIRDGITMEGIAGRKYNYFRALQLEQQERGVNLMPNTDVPILEEWNQADYLP
EEEEEEEEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHHCCCCCCCCC
PEVNEVLNRYKEGKGLDTKMRQTDPTLKSISDSKNTDAAEVDKQSTELEHTGTDKDSSDE
HHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCCCHHHHCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]