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The map label for this gene is epsE [H]

Identifier: 167626078

GI number: 167626078

Start: 5071956

End: 5073527

Strand: Reverse

Name: epsE [H]

Synonym: Shal_4173

Alternate gene names: 167626078

Gene position: 5073527-5071956 (Counterclockwise)

Preceding gene: 167626079

Following gene: 167626077

Centisome position: 97.07

GC content: 47.84

Gene sequence:

>1572_bases
ATGAATGATAATCAAATTCCGGTCGATGAAGAGCTAGCGCAAGTATCGAGTGAGCTTGGGCTGGTGTCTGAGGAAGAAGC
CGATCAAGTCTTCCATTCGAGCAGCAAAGAGCGATTGCCGTTTGCCTTTGCCCATCGCTTTAAGGTTGCGCTTGCTGTCG
ATGATGAAGGCAAGTTGTCGCTATATTATACTCAAGGCACGCCACTGAGTGCCCTGCTGGAAGTGCGCCGCTACTGCGGT
AAAGAGTTACCACTGATAAAGCTAGAAGACGCTGCCTTTGAGGCGCGATTGACACAAGCTTATCAGGCAAACTCTTCTGA
AGCGCAGCAGATGATGGAAGATATCGGTAATGAGATGGACTTGTTCACTCTTGCCGAAGAACTACCGCAAACTGAAGATC
TGCTAGAGGGTGATGATGATGCGCCGATTATTAAGTTAATTAATGCACTATTATCTGAAGCCATCAAAGAAGAAGCCTCC
GATATTCATATCGAAACCTATGAGAAGCAGTTGATTGTTCGCTTCCGTGTCGATGGCATTTTGAAAGAAGTCCTTAAACC
TAATCGTAAGCTGTCATCTCTGTTGGTATCACGTATCAAGGTTATGGCGCGTCTCGATATTGCCGAAAAACGGGTACCGC
AAGATGGCCGTATTTCGCTGCGTATCGCCGGTCGCGCCGTCGATGTGCGTGTATCTACCATGCCATCAAGTCATGGTGAG
CGAGTGGTACTGCGTCTACTTGATAAGAATACGGGTAATTTAGATTTAGAGCAGCTCGGTATGACCGAGTCTGTACGTCA
TCAGTTTGATGAACTTATCCGTAGACCTCACGGCATTATTTTGGTGACCGGTCCGACAGGCTCGGGTAAGAGTACCACGC
TATACGCGGGTCTTACCGAGCTGAATTCAAAAGACACCAACATTCTTACCGTTGAAGACCCGATAGAATATGAGCTTGAA
GGCATAGGTCAGACTCAGGTTAACACTAAGGTCGATATGACTTTCGCTCGTGGCTTACGTGCTATTTTGCGTCAAGATCC
TGATGTGGTGATGATTGGTGAAATTCGAGATCTTGAAACTGCACAGATTGCGGTTCAAGCGTCACTTACTGGTCATATGG
TTATCTCAACCTTACATACCAATACCGCATCGGGCGCCATTACTCGTCTGCAAGATATGGGGGTTGAACCTTTCCTTGTG
TCTTCGAGTTTACTCGGTGTGCTAGCGCAGCGTCTTATTCGAACTCTGTGCTCAAGCTGTAAAGAGGAGCACACCCCGGA
TGATCGTGAAAGAGAATTGTTAGGTATTACCGCTGACGATCCGCGGGTTATTTTCCGCGCTAAAGGCTGTCAGGCTTGTG
GTCATAATGGCTATCGTGGCCGTACAGGTATTCATGAGCTATTAACCGTCGATGATACCGTGCGTGAACTTATCCATACT
GGCCGAGGCGAGCTCGCTATTGAGAAACACATTCGTCAATCAGTACCGAGTATTCGTCACGACGGTATGAGTAAGGTGCT
TGCGGGTAAAACCACCCTCGAAGAAGTGCTGCGAGTCACTCGAGAGGAGTAG

Upstream 100 bases:

>100_bases
ACACTTAAGAGTATTTCAGACAGTAAGAATACCGATGCTGCTGAAGTGGATAAGCAGTCGACAGAGTTAGAACATACTGG
TACGGATAAAGATTCAAGCG

Downstream 100 bases:

>100_bases
TCATGCCCGCATTTGAATACAAAGCATTAGACAAGACGGGTAAGCAGCAAAAGGGCGTGGTCGAGGCCGACACCGCCAGA
CATGCCCGCACCCAGTTAAG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNDNQIPVDEELAQVSSELGLVSEEEADQVFHSSSKERLPFAFAHRFKVALAVDDEGKLSLYYTQGTPLSALLEVRRYCG
KELPLIKLEDAAFEARLTQAYQANSSEAQQMMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEAS
DIHIETYEKQLIVRFRVDGILKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
RVVLRLLDKNTGNLDLEQLGMTESVRHQFDELIRRPHGIILVTGPTGSGKSTTLYAGLTELNSKDTNILTVEDPIEYELE
GIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLV
SSSLLGVLAQRLIRTLCSSCKEEHTPDDRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLTVDDTVRELIHT
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE

Sequences:

>Translated_523_residues
MNDNQIPVDEELAQVSSELGLVSEEEADQVFHSSSKERLPFAFAHRFKVALAVDDEGKLSLYYTQGTPLSALLEVRRYCG
KELPLIKLEDAAFEARLTQAYQANSSEAQQMMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEAS
DIHIETYEKQLIVRFRVDGILKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
RVVLRLLDKNTGNLDLEQLGMTESVRHQFDELIRRPHGIILVTGPTGSGKSTTLYAGLTELNSKDTNILTVEDPIEYELE
GIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLV
SSSLLGVLAQRLIRTLCSSCKEEHTPDDRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLTVDDTVRELIHT
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE
>Mature_523_residues
MNDNQIPVDEELAQVSSELGLVSEEEADQVFHSSSKERLPFAFAHRFKVALAVDDEGKLSLYYTQGTPLSALLEVRRYCG
KELPLIKLEDAAFEARLTQAYQANSSEAQQMMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEAS
DIHIETYEKQLIVRFRVDGILKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
RVVLRLLDKNTGNLDLEQLGMTESVRHQFDELIRRPHGIILVTGPTGSGKSTTLYAGLTELNSKDTNILTVEDPIEYELE
GIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLV
SSSLLGVLAQRLIRTLCSSCKEEHTPDDRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLTVDDTVRELIHT
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=479, Percent_Identity=57.4112734864301, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=42.96875, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=46.7455621301775, Blast_Score=128, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58112; Mature: 58112

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNQIPVDEELAQVSSELGLVSEEEADQVFHSSSKERLPFAFAHRFKVALAVDDEGKLS
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHEEEEEEEEEEECCCCCEE
LYYTQGTPLSALLEVRRYCGKELPLIKLEDAAFEARLTQAYQANSSEAQQMMEDIGNEMD
EEEECCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
LFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLIVRFRVDGI
HHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHEEEHHHHHHH
LKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCC
RVVLRLLDKNTGNLDLEQLGMTESVRHQFDELIRRPHGIILVTGPTGSGKSTTLYAGLTE
EEEHHEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHH
LNSKDTNILTVEDPIEYELEGIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLET
CCCCCCEEEEECCCCCHHHCCCCCEECCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCH
AQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCSSC
HHEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KEEHTPDDRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLTVDDTVRELIHT
HHCCCCCCCCCEEEEEECCCCEEEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE
CCCCHHHHHHHHHHCCHHHHCCHHHHHHCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDNQIPVDEELAQVSSELGLVSEEEADQVFHSSSKERLPFAFAHRFKVALAVDDEGKLS
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHEEEEEEEEEEECCCCCEE
LYYTQGTPLSALLEVRRYCGKELPLIKLEDAAFEARLTQAYQANSSEAQQMMEDIGNEMD
EEEECCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
LFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLIVRFRVDGI
HHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHEEEHHHHHHH
LKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCC
RVVLRLLDKNTGNLDLEQLGMTESVRHQFDELIRRPHGIILVTGPTGSGKSTTLYAGLTE
EEEHHEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHH
LNSKDTNILTVEDPIEYELEGIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLET
CCCCCCEEEEECCCCCHHHCCCCCEECCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCH
AQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCSSC
HHEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KEEHTPDDRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLTVDDTVRELIHT
HHCCCCCCCCCEEEEEECCCCEEEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE
CCCCHHHHHHHHHHCCHHHHCCHHHHHHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]