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The map label for this gene is nudE [H]

Identifier: 167626067

GI number: 167626067

Start: 5063690

End: 5064250

Strand: Reverse

Name: nudE [H]

Synonym: Shal_4162

Alternate gene names: 167626067

Gene position: 5064250-5063690 (Counterclockwise)

Preceding gene: 167626069

Following gene: 167626066

Centisome position: 96.89

GC content: 43.49

Gene sequence:

>561_bases
ATGTGTATGACTAAAAAAAATGAGAAACCTGAAATTTTGCACGCTGAAGTCGTTGCTAGAAGTCGACTGTTTCAAATTGA
ACGAGTGCACTTGAGGTTTTCCAATCAAGTCGAGCGTCAGTATGAGCGTATGAAAGGCAATAATCGCGGTGCGGTAATGG
TTGTGCCTGTGCTCAACGGTACTGAGCTGTTGTTAGGTCGAGAATATGCTGCAGGTACTCATAGCTATGAGCTAGGGTTT
CCTAAGGGGTTGATCGATCCCGGAGAAGAGGCCCATGAAGCAGCAAATCGCGAGTTACAGGAAGAGATAGGTTATGGGGC
GAGAAAACTGACCCATTTAATGGAGCTAAGCCTAGCACCGGGTTATTTTGCTAGTAAAATGCAGATATTTTTAGCTGAAG
ACCTTTACGAAAGTATCCTCGAAGGCGATGAGCCAGAACCTATCGATATGGTTCCTTGGCCATTAACTGAATGGCAAGAT
CTACTGAATGAGGACGATTTTTCAGAGTCTCGTAGTGTAAGTGCTTTATTTTTAGCGCAAAAACATCTACAACTAAGATA
G

Upstream 100 bases:

>100_bases
GGAAACATAGTTACGCGATCCTAGAAAATTCATGTTGTAGATTGGGTGAACAATAGTAAGATTATACTGAATTAAATGAT
AAGTCGGCAGCGGCACTGGA

Downstream 100 bases:

>100_bases
ACAAAACGACGCAGGTCATGGGTGTTAGGCTGTTGGAGTAGTTATGAATCCAGAGAATTATGTAGATCAAGTCATTGAAA
TCGCAACATTAGCAGGCAAG

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MCMTKKNEKPEILHAEVVARSRLFQIERVHLRFSNQVERQYERMKGNNRGAVMVVPVLNGTELLLGREYAAGTHSYELGF
PKGLIDPGEEAHEAANRELQEEIGYGARKLTHLMELSLAPGYFASKMQIFLAEDLYESILEGDEPEPIDMVPWPLTEWQD
LLNEDDFSESRSVSALFLAQKHLQLR

Sequences:

>Translated_186_residues
MCMTKKNEKPEILHAEVVARSRLFQIERVHLRFSNQVERQYERMKGNNRGAVMVVPVLNGTELLLGREYAAGTHSYELGF
PKGLIDPGEEAHEAANRELQEEIGYGARKLTHLMELSLAPGYFASKMQIFLAEDLYESILEGDEPEPIDMVPWPLTEWQD
LLNEDDFSESRSVSALFLAQKHLQLR
>Mature_186_residues
MCMTKKNEKPEILHAEVVARSRLFQIERVHLRFSNQVERQYERMKGNNRGAVMVVPVLNGTELLLGREYAAGTHSYELGF
PKGLIDPGEEAHEAANRELQEEIGYGARKLTHLMELSLAPGYFASKMQIFLAEDLYESILEGDEPEPIDMVPWPLTEWQD
LLNEDDFSESRSVSALFLAQKHLQLR

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=182, Percent_Identity=60.4395604395604, Blast_Score=221, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21302; Mature: 21302

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMTKKNEKPEILHAEVVARSRLFQIERVHLRFSNQVERQYERMKGNNRGAVMVVPVLNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
TELLLGREYAAGTHSYELGFPKGLIDPGEEAHEAANRELQEEIGYGARKLTHLMELSLAP
CEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GYFASKMQIFLAEDLYESILEGDEPEPIDMVPWPLTEWQDLLNEDDFSESRSVSALFLAQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
KHLQLR
HHHCCC
>Mature Secondary Structure
MCMTKKNEKPEILHAEVVARSRLFQIERVHLRFSNQVERQYERMKGNNRGAVMVVPVLNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
TELLLGREYAAGTHSYELGFPKGLIDPGEEAHEAANRELQEEIGYGARKLTHLMELSLAP
CEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GYFASKMQIFLAEDLYESILEGDEPEPIDMVPWPLTEWQDLLNEDDFSESRSVSALFLAQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
KHLQLR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]