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The map label for this gene is leuA

Identifier: 167622378

GI number: 167622378

Start: 514383

End: 515954

Strand: Direct

Name: leuA

Synonym: Shal_0438

Alternate gene names: 167622378

Gene position: 514383-515954 (Clockwise)

Preceding gene: 167622376

Following gene: 167622379

Centisome position: 9.84

GC content: 48.16

Gene sequence:

>1572_bases
ATGTCTAACCGAGTGATTATATTTGACACCACCTTACGCGATGGTGAGCAAGCATTAGCAGCAAGTTTAACGGTTAAAGA
AAAGCTTCAGATTGCACTGGCGTTGGAGCGTTTAGGTGTTGATGTGATGGAGGTGGGGTTCCCAGTCTCTTCACCAGGTG
ATTTTGAGTCGGTGCAAACCATTGCAAAAACGATTAAGAATAGCCGCGTATGTGCATTGTCGAGGGCGTTAGAAAGTGAT
ATCGACGCCGCGGCTCAGGCTTTGTCGGTAGCGGAACAGTTCCGTATTCATACGTTTATCTCGACATCGACCATTCATGT
TGAAAACAAGTTAAAGCGCTCATTCGATCAAGTGCTCGATATGGCGGTAGGAGCGGTTAAGTACGCGCGCCGATTTACCG
ATGATGTGGAGTTCTCTTGTGAAGATGCCGGTCGTACCCCTATCGATAATTTGTGCCGTATGGTGGAAGAGGCCATTAAG
GCTGGAGCGCGCACCATTAATATTCCTGACACTGTTGGTTATACGGTGCCAAGCGAATTTGGCGGCATTATTCAAACCTT
GTTTAACCGCGTCCCCAATATTGATCAAGCGGTGATCTCGGTGCATTGCCATGATGACTTAGGCTTGTCAGTCGCAAACT
CTATTACCGCAGTGCAGCACGGTGCTCGCCAAATTGAATGTACCGTAAACGGCATTGGTGAGCGCGCGGGTAACTGCTCG
CTAGAAGAGATCGCCATGATCTTGTCGACCCGCAAAGGTGAGTTAGGCCTAGAGACGGGGATCAACGCTAAAGAGATCCA
CCGCACTTCAAGCTTAGTGAGCCAGCTTTGTAATATGCCAGTGCAAGCAAACAAGGCGATAGTGGGGGCGAATGCCTTTA
CTCACTCATCGGGTATTCATCAAGACGGCATGCTAAAAGCGCAAAATACCTATGAGATTATGACGCCTGAGAGTATTGGC
CTTAACCGTAATAACTTAAACATGACCTCACGCTCAGGCCGCCACGTTATCAAGCATCGTATGAGTGAGCTTGGCTATGG
TGAGCAAGATTACGATATGGATGTTTTGTATGAAGAGTTTTTAGCTCTAGCAGATAAAAAGGGTCAAGTGTTTGATTATG
ACTTAGAAGCATTGGCCTTTATGGAGGCGCAGGCTGAAGACGATGATCATTTTGAACTGCAGCAATTGGTTGTGCATTCA
GACTCAACCGAAGGCAGCGCAACAGCCACGGTAAAAGTGGCGGTTAATGGTGAGACGATTACCGAAGCCGCAACGGGTAA
CGGTCCTGTCGATGCGGCGTATAAGGCGGTTGCCCGTGCTAGTGGCTGTAAAACCAACATCACCAGTTACCAGTTAAGTG
CAAAAGGCGAAGGTCACAATGCGTTAGGCCAAGTGGATATTACCGCCAAGTATCGCGAGCAAAACTTCCATGGTGTGGGC
TTAGCAACTGATGTGGTTGAAGCCTCTGCCAAGGCCTTAATCCATGTGATGAACTTGACATGGCGCGCCGATAAAGTGGC
TGATTGCAAACAGCGTATCCAACAGAATAAACAAGAGTTCGGCAGCGTATAA

Upstream 100 bases:

>100_bases
CTAATAACGCGGGGTTTTTTGTATTTATAGCAATCGGTATTAAACGCCAAATAGCTACGAGAACGCATAACCGAACAATG
ATGTTCAATGGAGAAGATAG

Downstream 100 bases:

>100_bases
GCTTGCCCATTTAATGTCACCCAATACTTATAAATTGAAAATTAAATTAAAGAGCAAATTACAGGAGTTAGCTGACGTAT
GAGTTATCAGATTGCAGTAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQTIAKTIKNSRVCALSRALESD
IDAAAQALSVAEQFRIHTFISTSTIHVENKLKRSFDQVLDMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIK
AGARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS
LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIG
LNRNNLNMTSRSGRHVIKHRMSELGYGEQDYDMDVLYEEFLALADKKGQVFDYDLEALAFMEAQAEDDDHFELQQLVVHS
DSTEGSATATVKVAVNGETITEAATGNGPVDAAYKAVARASGCKTNITSYQLSAKGEGHNALGQVDITAKYREQNFHGVG
LATDVVEASAKALIHVMNLTWRADKVADCKQRIQQNKQEFGSV

Sequences:

>Translated_523_residues
MSNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQTIAKTIKNSRVCALSRALESD
IDAAAQALSVAEQFRIHTFISTSTIHVENKLKRSFDQVLDMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIK
AGARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS
LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIG
LNRNNLNMTSRSGRHVIKHRMSELGYGEQDYDMDVLYEEFLALADKKGQVFDYDLEALAFMEAQAEDDDHFELQQLVVHS
DSTEGSATATVKVAVNGETITEAATGNGPVDAAYKAVARASGCKTNITSYQLSAKGEGHNALGQVDITAKYREQNFHGVG
LATDVVEASAKALIHVMNLTWRADKVADCKQRIQQNKQEFGSV
>Mature_522_residues
SNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQTIAKTIKNSRVCALSRALESDI
DAAAQALSVAEQFRIHTFISTSTIHVENKLKRSFDQVLDMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIKA
GARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCSL
EEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIGL
NRNNLNMTSRSGRHVIKHRMSELGYGEQDYDMDVLYEEFLALADKKGQVFDYDLEALAFMEAQAEDDDHFELQQLVVHSD
STEGSATATVKVAVNGETITEAATGNGPVDAAYKAVARASGCKTNITSYQLSAKGEGHNALGQVDITAKYREQNFHGVGL
ATDVVEASAKALIHVMNLTWRADKVADCKQRIQQNKQEFGSV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=518, Percent_Identity=65.4440154440154, Blast_Score=700, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320019, Length=350, Percent_Identity=28.2857142857143, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=535, Percent_Identity=25.2336448598131, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320071, Length=346, Percent_Identity=28.3236994219653, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=25.2788104089219, Blast_Score=124, Evalue=4e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_SHEHH (B0TQM0)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672672.1
- ProteinModelPortal:   B0TQM0
- SMR:   B0TQM0
- GeneID:   5905667
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0438
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   SHAL458817:SHAL_0438-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56788; Mature: 56656

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IAKTIKNSRVCALSRALESDIDAAAQALSVAEQFRIHTFISTSTIHVENKLKRSFDQVLD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEHHHHHHHHHHHHHH
MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIKAGARTINIPDTVGYTVPSEF
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIH
HHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEECCCCCC
QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMSELGYGEQDYDMDVLYEEF
CCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LALADKKGQVFDYDLEALAFMEAQAEDDDHFELQQLVVHSDSTEGSATATVKVAVNGETI
HHHHCCCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCEE
TEAATGNGPVDAAYKAVARASGCKTNITSYQLSAKGEGHNALGQVDITAKYREQNFHGVG
ECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCC
LATDVVEASAKALIHVMNLTWRADKVADCKQRIQQNKQEFGSV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IAKTIKNSRVCALSRALESDIDAAAQALSVAEQFRIHTFISTSTIHVENKLKRSFDQVLD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEHHHHHHHHHHHHHH
MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIKAGARTINIPDTVGYTVPSEF
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIH
HHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEECCCCCC
QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMSELGYGEQDYDMDVLYEEF
CCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LALADKKGQVFDYDLEALAFMEAQAEDDDHFELQQLVVHSDSTEGSATATVKVAVNGETI
HHHHCCCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCEE
TEAATGNGPVDAAYKAVARASGCKTNITSYQLSAKGEGHNALGQVDITAKYREQNFHGVG
ECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCC
LATDVVEASAKALIHVMNLTWRADKVADCKQRIQQNKQEFGSV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA