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The map label for this gene is rpoH [H]

Identifier: 167622231

GI number: 167622231

Start: 346897

End: 347754

Strand: Direct

Name: rpoH [H]

Synonym: Shal_0290

Alternate gene names: 167622231

Gene position: 346897-347754 (Clockwise)

Preceding gene: 167622230

Following gene: 167622233

Centisome position: 6.64

GC content: 45.45

Gene sequence:

>858_bases
ATGACAGTTCAAACGCAAACAATGGCACTGATGGTTCCTCAGAGTAGTGGAAGCCTCGAGTCTTATGTTCATTCAGCACA
CAACATTCCGATGTTGGAAGCCGATACAGAATATGAGCTAGCGAAGCGTCTACAGGAGACCGGTGACCTACAGGCTGCGA
AACAGTTGATTATGTCGCACCTGCGTTTCGTAGTGCATGTTGCAAAGGGTTATTCTGGTTATGGTTTACCGCAAGCTGAC
TTAATTCAAGAAGGTAATATCGGCCTGATGAAAGCGGTTAAGCGTTTCGACCCTGATGTGGGCGTGCGCTTGGTATCGTT
TGCCGTGCATTGGATTAAAGCTGAAATTCATGAGTATGTGCTTAAAAACTGGCGCATCGTAAAAGTTGCCACCACCAAAG
CGCAGCGCAAGTTATTCTTCAATCTTCGTAAAGCTAAAAAGCGCTTAGGTTGGTTTAGCGATAGTGAAGTGGGAATGGTG
GCCGACAGTTTAGGGGTGTCGAAAGCCGATGTGACCGAGATGGAATCACGTATGGCAGCGCAAGATCCAGCATTTGATCT
TGCTAGCGACAATGATGACGAGCATGATTATGCACCTATGCATTATCTTGAAGATCATTCATCAGACTTAGCTTCTCAAG
TTGAAAGTGACAACTGGGAACAGAATAATCAAGCGCGTTTGCTATCGGCCATCAAGACTCTTGATGAGCGTAGCCAACAT
ATCCTTAAGGCGCGCTGGTTAGATGAAGATAAGACCACATTGCAAGATTTAGCATCGACCTATCAAGTGTCTGCTGAACG
TATTCGACAGTTGGAAAAGAATGCGATGAGTAAGCTGAAAGGGTGTATGGAGGTTTAA

Upstream 100 bases:

>100_bases
TTTGTCAATTAGTCGATAGTTCACAGTCCATTTCAGCAATGAGTGGATCCGCAAGTAGATTTTTAGCTAGACCTTTTGTA
AGAAAGTAGTAGGAGCGTAA

Downstream 100 bases:

>100_bases
CTCCACTAAGCCGCTTCACTAGTTTTAGCGATAATTGAAACCTGCTTACCCAAGCAGGTTTTTTTATGTCTGGAACATTT
ATGCCAAAAGCCCATGGATG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTVQTQTMALMVPQSSGSLESYVHSAHNIPMLEADTEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQAD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMV
ADSLGVSKADVTEMESRMAAQDPAFDLASDNDDEHDYAPMHYLEDHSSDLASQVESDNWEQNNQARLLSAIKTLDERSQH
ILKARWLDEDKTTLQDLASTYQVSAERIRQLEKNAMSKLKGCMEV

Sequences:

>Translated_285_residues
MTVQTQTMALMVPQSSGSLESYVHSAHNIPMLEADTEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQAD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMV
ADSLGVSKADVTEMESRMAAQDPAFDLASDNDDEHDYAPMHYLEDHSSDLASQVESDNWEQNNQARLLSAIKTLDERSQH
ILKARWLDEDKTTLQDLASTYQVSAERIRQLEKNAMSKLKGCMEV
>Mature_284_residues
TVQTQTMALMVPQSSGSLESYVHSAHNIPMLEADTEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQADL
IQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMVA
DSLGVSKADVTEMESRMAAQDPAFDLASDNDDEHDYAPMHYLEDHSSDLASQVESDNWEQNNQARLLSAIKTLDERSQHI
LKARWLDEDKTTLQDLASTYQVSAERIRQLEKNAMSKLKGCMEV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=286, Percent_Identity=66.0839160839161, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1789098, Length=260, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=27.3504273504274, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32257; Mature: 32126

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQTQTMALMVPQSSGSLESYVHSAHNIPMLEADTEYELAKRLQETGDLQAAKQLIMSH
CCCCCCEEEEEEECCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHH
LRFVVHVAKGYSGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
HHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMVADSLGVSKADVTEMESRMAA
HHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
QDPAFDLASDNDDEHDYAPMHYLEDHSSDLASQVESDNWEQNNQARLLSAIKTLDERSQH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
ILKARWLDEDKTTLQDLASTYQVSAERIRQLEKNAMSKLKGCMEV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVQTQTMALMVPQSSGSLESYVHSAHNIPMLEADTEYELAKRLQETGDLQAAKQLIMSH
CCCCCEEEEEEECCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHH
LRFVVHVAKGYSGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
HHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMVADSLGVSKADVTEMESRMAA
HHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
QDPAFDLASDNDDEHDYAPMHYLEDHSSDLASQVESDNWEQNNQARLLSAIKTLDERSQH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
ILKARWLDEDKTTLQDLASTYQVSAERIRQLEKNAMSKLKGCMEV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]