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The map label for this gene is moaA [H]

Identifier: 167622085

GI number: 167622085

Start: 162347

End: 163333

Strand: Reverse

Name: moaA [H]

Synonym: Shal_0144

Alternate gene names: 167622085

Gene position: 163333-162347 (Counterclockwise)

Preceding gene: 167622086

Following gene: 167622082

Centisome position: 3.12

GC content: 49.85

Gene sequence:

>987_bases
ATGAGTTTAATCGTTGACACTTTTGGTCGCACGGTAGAATACCTGCGGCTATCCGTCACAGACCGCTGCGACTTTCGCTG
CGTGTACTGCATGAGTGAAGATCCCTGCTTTTTAGGCCGGGAACATGTACTTAGCCTAGAGGAATTGGCTTGGGTCGCCC
AAGCCTTTACCGAATTAGGTGTTAAGAAAATTCGTTTAACTGGTGGTGAGCCCTTAGTGCGCACTGACTGCGATCAGCTA
GTGAAGCTATTGGGTAAGTTACCAGGGCTTGAAGAGTTGTCGATGACAACTAATGGATCGCGCCTGACCCGCTTCGCGCA
AGAGATGCACGATTCCGGTTTAAATCGACTCAACATCAGTCTAGATACGTTAAAACCGGACCTATTCACAGAGCTGACTC
GTAACGGCAAAGTCGATCGCGTTATCGCCGGTATCGATGCCGCTATTGCCGCAGGCTTTAAGAAGATCAAGGTCAATGCG
GTGATCCTGCGCGGCCAAAACGATGATGAAGTCTTAGATCTGATTGAGTTTTGCCGCGAACGCGACTTAGATATCGCCTT
CATCGAAGAGATGCCACTTGGGGTGATTGATGAGCGTAAGCGCAGCCGTCACTGCAGCAGCAAAGAGATCCAAGAGATCA
TCAAACAGCGTTATGAGCTCATCCAGTCCAATAGACGCACCGGCGGTCCAGCGCGTTACTACACCATGCCCGGCAGCGAT
ATTCATGTGGGCTTTATCTCGCCTCACAGCAATAACTTCTGCCACGAGTGTAACCGCGTCCGCGTCACGGTAGAGGGCAA
ACTGCTACTCTGCTTAGGTAATGAAAACTCGGTCGATCTCAAAGCGATTGTGCGTGAATTCCCCGGTGATATTGAAAAGC
TCAAAGTCGCGATTCTAGAAGGCATTCAGCACAAGCCAAAAGAGCACCATTTTGATCCCAATGGTGAAACGCAGATCTTG
CGGTTTATGAATGCAACAGGTGGTTGA

Upstream 100 bases:

>100_bases
TCATCGTGATTGGCGAGAAAGATGAACAGTTAAATCCAGGTGACACAGTTTACATTCAACCTTTTGCCGACCTCCTTTAG
GGGTCATGTAGGTAGTTAGT

Downstream 100 bases:

>100_bases
TTTAACTGTTGCTTGAACAGTTAATTGTCGCTTGTTTGGTGGTCTGGTGTTTTCTGCAGCCACAGAGTTTGATTGTTAGC
TTGTGCTATATACACAAAAC

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSLIVDTFGRTVEYLRLSVTDRCDFRCVYCMSEDPCFLGREHVLSLEELAWVAQAFTELGVKKIRLTGGEPLVRTDCDQL
VKLLGKLPGLEELSMTTNGSRLTRFAQEMHDSGLNRLNISLDTLKPDLFTELTRNGKVDRVIAGIDAAIAAGFKKIKVNA
VILRGQNDDEVLDLIEFCRERDLDIAFIEEMPLGVIDERKRSRHCSSKEIQEIIKQRYELIQSNRRTGGPARYYTMPGSD
IHVGFISPHSNNFCHECNRVRVTVEGKLLLCLGNENSVDLKAIVREFPGDIEKLKVAILEGIQHKPKEHHFDPNGETQIL
RFMNATGG

Sequences:

>Translated_328_residues
MSLIVDTFGRTVEYLRLSVTDRCDFRCVYCMSEDPCFLGREHVLSLEELAWVAQAFTELGVKKIRLTGGEPLVRTDCDQL
VKLLGKLPGLEELSMTTNGSRLTRFAQEMHDSGLNRLNISLDTLKPDLFTELTRNGKVDRVIAGIDAAIAAGFKKIKVNA
VILRGQNDDEVLDLIEFCRERDLDIAFIEEMPLGVIDERKRSRHCSSKEIQEIIKQRYELIQSNRRTGGPARYYTMPGSD
IHVGFISPHSNNFCHECNRVRVTVEGKLLLCLGNENSVDLKAIVREFPGDIEKLKVAILEGIQHKPKEHHFDPNGETQIL
RFMNATGG
>Mature_327_residues
SLIVDTFGRTVEYLRLSVTDRCDFRCVYCMSEDPCFLGREHVLSLEELAWVAQAFTELGVKKIRLTGGEPLVRTDCDQLV
KLLGKLPGLEELSMTTNGSRLTRFAQEMHDSGLNRLNISLDTLKPDLFTELTRNGKVDRVIAGIDAAIAAGFKKIKVNAV
ILRGQNDDEVLDLIEFCRERDLDIAFIEEMPLGVIDERKRSRHCSSKEIQEIIKQRYELIQSNRRTGGPARYYTMPGSDI
HVGFISPHSNNFCHECNRVRVTVEGKLLLCLGNENSVDLKAIVREFPGDIEKLKVAILEGIQHKPKEHHFDPNGETQILR
FMNATGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=310, Percent_Identity=36.4516129032258, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI22027536, Length=310, Percent_Identity=36.4516129032258, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=35.258358662614, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI32566331, Length=316, Percent_Identity=35.126582278481, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17561210, Length=307, Percent_Identity=36.4820846905537, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI32566329, Length=307, Percent_Identity=35.5048859934853, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28574490, Length=312, Percent_Identity=41.025641025641, Blast_Score=220, Evalue=8e-58,
Organism=Drosophila melanogaster, GI28574488, Length=312, Percent_Identity=41.025641025641, Blast_Score=220, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37059; Mature: 36928

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIVDTFGRTVEYLRLSVTDRCDFRCVYCMSEDPCFLGREHVLSLEELAWVAQAFTELG
CCEEHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
VKKIRLTGGEPLVRTDCDQLVKLLGKLPGLEELSMTTNGSRLTRFAQEMHDSGLNRLNIS
CEEEEECCCCCCHHCCHHHHHHHHHCCCCCCHHEECCCCHHHHHHHHHHHHCCCCEEEEE
LDTLKPDLFTELTRNGKVDRVIAGIDAAIAAGFKKIKVNAVILRGQNDDEVLDLIEFCRE
HHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
RDLDIAFIEEMPLGVIDERKRSRHCSSKEIQEIIKQRYELIQSNRRTGGPARYYTMPGSD
CCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCE
IHVGFISPHSNNFCHECNRVRVTVEGKLLLCLGNENSVDLKAIVREFPGDIEKLKVAILE
EEEEEECCCCCCHHCCCCEEEEEEECEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHH
GIQHKPKEHHFDPNGETQILRFMNATGG
HHCCCCHHCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SLIVDTFGRTVEYLRLSVTDRCDFRCVYCMSEDPCFLGREHVLSLEELAWVAQAFTELG
CEEHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
VKKIRLTGGEPLVRTDCDQLVKLLGKLPGLEELSMTTNGSRLTRFAQEMHDSGLNRLNIS
CEEEEECCCCCCHHCCHHHHHHHHHCCCCCCHHEECCCCHHHHHHHHHHHHCCCCEEEEE
LDTLKPDLFTELTRNGKVDRVIAGIDAAIAAGFKKIKVNAVILRGQNDDEVLDLIEFCRE
HHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
RDLDIAFIEEMPLGVIDERKRSRHCSSKEIQEIIKQRYELIQSNRRTGGPARYYTMPGSD
CCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCE
IHVGFISPHSNNFCHECNRVRVTVEGKLLLCLGNENSVDLKAIVREFPGDIEKLKVAILE
EEEEEECCCCCCHHCCCCEEEEEEECEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHH
GIQHKPKEHHFDPNGETQILRFMNATGG
HHCCCCHHCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA