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The map label for this gene is fmt

Identifier: 167621967

GI number: 167621967

Start: 30950

End: 31915

Strand: Reverse

Name: fmt

Synonym: Shal_0026

Alternate gene names: 167621967

Gene position: 31915-30950 (Counterclockwise)

Preceding gene: 167621968

Following gene: 167621966

Centisome position: 0.61

GC content: 47.31

Gene sequence:

>966_bases
TTGAAACCATTAAATGTCATTTTTGCAGGAACGCCAGACTTTGCTGCACGCCATCTTCAAGCGCTAATCGATTCAGAGCA
TAATGTTATTGCGGTATATACTCAGCCTGATAGACCTGCTGGCCGCGGTAAAAAGCTTCAAGCTAGCCCAGTCAAAGCAT
TAGCGCTTGAAAATGGCATCGCAGTTTTTCAACCTAAATCGCTACGTGATGAAGATGCTCAAGCAGAGCTTACCGCCTTA
AATGCTGACATTATGGTTGTTGTCGCTTATGGGCTTATCTTGCCAAAAGTGGTACTCGATACGCCACGTCTTGGTTGCAT
CAATGTTCATGGTTCAATCTTGCCTCGCTGGCGTGGCGCCGCACCGATTCAGCGCGCACTATGGGCAGGCGACAGTGAAA
CTGGCGTCACCATTATGCAGATGGATATTGGTCTAGATACCGGTGATATGCTGTTAAAAACTCAGCTAAAAATCGAAGAC
AGCGATACCTCGGCAACCCTTTATGAAAAGCTAGCAGAGCAAGGCCCAAGTGCACTTATTGAAGCACTAGCGGGTATTGC
ACAAGATAGCCTGCCTGCAGAAAAACAAGATGAGAGCTTAGCCAATTACGCTGAGAAACTGTCAAAAGAGGAAGCGCGTC
TAGATTGGTCTAAATCGGCAACGGCATTATGGCGTGAGATCCGTGCCTTTAACCCTTGGCCTGTTAGTCACTACGAGCAT
GAAGGCAATACCATTAAAGTATGGCAGAGCCATGTGAGCGATGAGACCAGTTCAAAGTTACCGGGCACAATTTTGTCAGC
CGATAAAAACGGCATTAGCATTGCTACGGGTGAAGGCGTACTCATCATCACCCAGATGCAGCTTCCAGGTAAAAAGCCGT
TAAGTGTTGCCGACATCCTAAACTCTAGAGCAGACTGGTTTACCCCAGGCACTGTACTGACTAGCACCGACAATCAAGAG
GCTTAA

Upstream 100 bases:

>100_bases
GGCAAGCTATTTGTTGACTACCTGTCACCACTCAAGCGTCAACGTATCAAACAGAAGCTAGAAAAAGCCGCTCGTCTCGA
AGCCAAACAAGGATAAATTT

Downstream 100 bases:

>100_bases
TCGCTAATGAACTTAAGAGCTTTGGCCGCTAAGGTCGTTTTTCAGGTGCTAGAAAAAGGTATCTCACTTTCTGTGGCACT
GCCTGTTCAGCAAAAACATC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENGIAVFQPKSLRDEDAQAELTAL
NADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGAAPIQRALWAGDSETGVTIMQMDIGLDTGDMLLKTQLKIED
SDTSATLYEKLAEQGPSALIEALAGIAQDSLPAEKQDESLANYAEKLSKEEARLDWSKSATALWREIRAFNPWPVSHYEH
EGNTIKVWQSHVSDETSSKLPGTILSADKNGISIATGEGVLIITQMQLPGKKPLSVADILNSRADWFTPGTVLTSTDNQE
A

Sequences:

>Translated_321_residues
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENGIAVFQPKSLRDEDAQAELTAL
NADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGAAPIQRALWAGDSETGVTIMQMDIGLDTGDMLLKTQLKIED
SDTSATLYEKLAEQGPSALIEALAGIAQDSLPAEKQDESLANYAEKLSKEEARLDWSKSATALWREIRAFNPWPVSHYEH
EGNTIKVWQSHVSDETSSKLPGTILSADKNGISIATGEGVLIITQMQLPGKKPLSVADILNSRADWFTPGTVLTSTDNQE
A
>Mature_321_residues
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENGIAVFQPKSLRDEDAQAELTAL
NADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGAAPIQRALWAGDSETGVTIMQMDIGLDTGDMLLKTQLKIED
SDTSATLYEKLAEQGPSALIEALAGIAQDSLPAEKQDESLANYAEKLSKEEARLDWSKSATALWREIRAFNPWPVSHYEH
EGNTIKVWQSHVSDETSSKLPGTILSADKNGISIATGEGVLIITQMQLPGKKPLSVADILNSRADWFTPGTVLTSTDNQE
A

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=214, Percent_Identity=33.1775700934579, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI238814322, Length=317, Percent_Identity=28.0757097791798, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI164663775, Length=237, Percent_Identity=28.6919831223629, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789683, Length=311, Percent_Identity=64.3086816720257, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1788589, Length=284, Percent_Identity=29.5774647887324, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI133930964, Length=245, Percent_Identity=30.2040816326531, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319458, Length=279, Percent_Identity=26.8817204301075, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550868, Length=327, Percent_Identity=25.9938837920489, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28571984, Length=244, Percent_Identity=28.6885245901639, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585660, Length=338, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_SHEHH (B0TLC9)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672261.1
- ProteinModelPortal:   B0TLC9
- SMR:   B0TLC9
- GeneID:   5904521
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0026
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   CLSK905552
- BioCyc:   SHAL458817:SHAL_0026-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34780; Mature: 34780

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENGI
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHEEHHHCCE
AVFQPKSLRDEDAQAELTALNADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGA
EEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRALWAGDSETGVTIMQMDIGLDTGDMLLKTQLKIEDSDTSATLYEKLAEQGPSALI
CHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCHHHHH
EALAGIAQDSLPAEKQDESLANYAEKLSKEEARLDWSKSATALWREIRAFNPWPVSHYEH
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEC
EGNTIKVWQSHVSDETSSKLPGTILSADKNGISIATGEGVLIITQMQLPGKKPLSVADIL
CCCEEEEEHHHCCCCCCCCCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHH
NSRADWFTPGTVLTSTDNQEA
CCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENGI
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHEEHHHCCE
AVFQPKSLRDEDAQAELTALNADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGA
EEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRALWAGDSETGVTIMQMDIGLDTGDMLLKTQLKIEDSDTSATLYEKLAEQGPSALI
CHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCHHHHH
EALAGIAQDSLPAEKQDESLANYAEKLSKEEARLDWSKSATALWREIRAFNPWPVSHYEH
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEC
EGNTIKVWQSHVSDETSSKLPGTILSADKNGISIATGEGVLIITQMQLPGKKPLSVADIL
CCCEEEEEHHHCCCCCCCCCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHH
NSRADWFTPGTVLTSTDNQEA
CCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA