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The map label for this gene is rsmB [H]

Identifier: 167621966

GI number: 167621966

Start: 29663

End: 30943

Strand: Reverse

Name: rsmB [H]

Synonym: Shal_0025

Alternate gene names: 167621966

Gene position: 30943-29663 (Counterclockwise)

Preceding gene: 167621967

Following gene: 167621965

Centisome position: 0.59

GC content: 48.32

Gene sequence:

>1281_bases
ATGAACTTAAGAGCTTTGGCCGCTAAGGTCGTTTTTCAGGTGCTAGAAAAAGGTATCTCACTTTCTGTGGCACTGCCTGT
TCAGCAAAAACATCTCGACAGTGGTAAAGACAAGGCGCTTCTTGCCGAGCTTTGCTATGGCGTAATGCGTCATTTGCCAC
AGTTAGACAAACAAGTCAGCGACTGCATGAGCAAACAGATGAAAGGTAAGCAGCGCATAGTGCACCAACTACTGCTTGTT
GGTTGCTATCAGCTGTACTTCACTCGTATTCCTAGCCATGCGGCAATTTCTGAAACTGCCGAAGCCTGTCGTCAGCTAAA
GTTTGAGGGTTTAGTTAAGGTGGTCAATGGCGTCTTACGTAATATTCAGCGCCAAGAAAAGCCATTATCGACCGATAACG
ACACCTTAGCTTATAACACGCCAGCTTGGATCATTAAGCGTCTACAGGCAGCATATCCGGATAACTGGCAAACCGTGATC
GAGCAAAGCCATGAGCGCCCACCTATGTGGTTGCGTAACAACCAGCAGTCTCAAACTCGAGAGACCTACTTGCAGGCGCT
AGCAGGATTAGATATTGAGGCACAAGCAGGCAATAGCCCTGACTCAATCCTATTAAGCAGCCCACGAGATGTGATGCAGC
TACCGGGTTTTGAATCTGGCGCGGCATCGGTGCAAGACGGTGCAGCCCAATGGGCAGCCACATTACTGGCACCACAAGGT
GATGAGCTGGTACTCGATGCTTGTGCAGCTCCCGGTGGTAAAAGCTGTCACTTACTTGAGCTAGCACCGGATATTAAACT
GGTTGCGGTAGATTTTGATGCCAAACGTCTTGAACGTGTGCAGCAAAACCTCGATCGCTTGTCACTCAAAGCAGAACTAG
TACATGGCGATGCCGCCAATATCGACTCTTGGTGGCAAGGTGATAAGTTCGACCGTATCTTGCTCGACGCACCTTGTTCA
GCAACCGGCGTTATTCGTCGTCACCCAGATATTAAATGGCTAAGAAAGAACAACGACATCGAAGAGTTGGCACAGTTGCA
GTCGCAAATCTTCGATCATTGCTGGAAGTGGTTAAAGCCCGGTGGCACACTCTTATATGCCACATGTTCGATTTTACCAC
AGGAAAACAAAGATCAAGTCAGTGCCTTCTTAGCCAGAACACCTGACGCGACCTTAGTACCTATTGAACAACAAGCTAAC
CCAGATGATATTGGTTGGCAGATTGTTCCGGGGCAAGACAACATGGACGGCTTTTATTACGCTCGCCTAGTGAAGGATTA
G

Upstream 100 bases:

>100_bases
AAAGCCGTTAAGTGTTGCCGACATCCTAAACTCTAGAGCAGACTGGTTTACCCCAGGCACTGTACTGACTAGCACCGACA
ATCAAGAGGCTTAATCGCTA

Downstream 100 bases:

>100_bases
AACGGCAATGAAAATTATCATTTTGGGTGCGGGACAAGTTGGTGGCACGTTAGCCGAAAACTTAGTTGGCGAAAACAATG
ACATCACCATTGTCGATCAC

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNLRALAAKVVFQVLEKGISLSVALPVQQKHLDSGKDKALLAELCYGVMRHLPQLDKQVSDCMSKQMKGKQRIVHQLLLV
GCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLRNIQRQEKPLSTDNDTLAYNTPAWIIKRLQAAYPDNWQTVI
EQSHERPPMWLRNNQQSQTRETYLQALAGLDIEAQAGNSPDSILLSSPRDVMQLPGFESGAASVQDGAAQWAATLLAPQG
DELVLDACAAPGGKSCHLLELAPDIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAANIDSWWQGDKFDRILLDAPCS
ATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKPGGTLLYATCSILPQENKDQVSAFLARTPDATLVPIEQQAN
PDDIGWQIVPGQDNMDGFYYARLVKD

Sequences:

>Translated_426_residues
MNLRALAAKVVFQVLEKGISLSVALPVQQKHLDSGKDKALLAELCYGVMRHLPQLDKQVSDCMSKQMKGKQRIVHQLLLV
GCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLRNIQRQEKPLSTDNDTLAYNTPAWIIKRLQAAYPDNWQTVI
EQSHERPPMWLRNNQQSQTRETYLQALAGLDIEAQAGNSPDSILLSSPRDVMQLPGFESGAASVQDGAAQWAATLLAPQG
DELVLDACAAPGGKSCHLLELAPDIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAANIDSWWQGDKFDRILLDAPCS
ATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKPGGTLLYATCSILPQENKDQVSAFLARTPDATLVPIEQQAN
PDDIGWQIVPGQDNMDGFYYARLVKD
>Mature_426_residues
MNLRALAAKVVFQVLEKGISLSVALPVQQKHLDSGKDKALLAELCYGVMRHLPQLDKQVSDCMSKQMKGKQRIVHQLLLV
GCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLRNIQRQEKPLSTDNDTLAYNTPAWIIKRLQAAYPDNWQTVI
EQSHERPPMWLRNNQQSQTRETYLQALAGLDIEAQAGNSPDSILLSSPRDVMQLPGFESGAASVQDGAAQWAATLLAPQG
DELVLDACAAPGGKSCHLLELAPDIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAANIDSWWQGDKFDRILLDAPCS
ATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKPGGTLLYATCSILPQENKDQVSAFLARTPDATLVPIEQQAN
PDDIGWQIVPGQDNMDGFYYARLVKD

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=369, Percent_Identity=27.3712737127371, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI76150623, Length=369, Percent_Identity=27.3712737127371, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI32698918, Length=188, Percent_Identity=33.5106382978723, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI39995082, Length=191, Percent_Identity=31.413612565445, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI301336155, Length=191, Percent_Identity=31.413612565445, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI11545785, Length=320, Percent_Identity=26.875, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI40316918, Length=182, Percent_Identity=30.2197802197802, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI8922322, Length=221, Percent_Identity=30.316742081448, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI270288816, Length=221, Percent_Identity=30.316742081448, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI270288818, Length=221, Percent_Identity=30.316742081448, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI23199998, Length=221, Percent_Identity=30.316742081448, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI2367212, Length=428, Percent_Identity=52.1028037383178, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI87081985, Length=221, Percent_Identity=31.6742081447964, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=311, Percent_Identity=26.6881028938907, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25143471, Length=181, Percent_Identity=34.8066298342541, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17509941, Length=165, Percent_Identity=28.4848484848485, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71998419, Length=172, Percent_Identity=31.3953488372093, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI115534021, Length=172, Percent_Identity=31.3953488372093, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=309, Percent_Identity=26.8608414239482, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319447, Length=187, Percent_Identity=31.5508021390374, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI22024126, Length=231, Percent_Identity=30.3030303030303, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19920494, Length=175, Percent_Identity=31.4285714285714, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24668781, Length=176, Percent_Identity=32.3863636363636, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21356513, Length=176, Percent_Identity=32.3863636363636, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355201, Length=178, Percent_Identity=31.4606741573034, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47560; Mature: 47560

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRALAAKVVFQVLEKGISLSVALPVQQKHLDSGKDKALLAELCYGVMRHLPQLDKQVS
CCHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DCMSKQMKGKQRIVHQLLLVGCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIQRQEKPLSTDNDTLAYNTPAWIIKRLQAAYPDNWQTVIEQSHERPPMWLRNNQQSQTR
HHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCHHHHH
ETYLQALAGLDIEAQAGNSPDSILLSSPRDVMQLPGFESGAASVQDGAAQWAATLLAPQG
HHHHHHHHCCCEEECCCCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
DELVLDACAAPGGKSCHLLELAPDIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAAN
CCEEHEECCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHEECCCCCC
IDSWWQGDKFDRILLDAPCSATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKP
CCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCC
GGTLLYATCSILPQENKDQVSAFLARTPDATLVPIEQQANPDDIGWQIVPGQDNMDGFYY
CCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCEEE
ARLVKD
EEEECC
>Mature Secondary Structure
MNLRALAAKVVFQVLEKGISLSVALPVQQKHLDSGKDKALLAELCYGVMRHLPQLDKQVS
CCHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DCMSKQMKGKQRIVHQLLLVGCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIQRQEKPLSTDNDTLAYNTPAWIIKRLQAAYPDNWQTVIEQSHERPPMWLRNNQQSQTR
HHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCHHHHH
ETYLQALAGLDIEAQAGNSPDSILLSSPRDVMQLPGFESGAASVQDGAAQWAATLLAPQG
HHHHHHHHCCCEEECCCCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
DELVLDACAAPGGKSCHLLELAPDIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAAN
CCEEHEECCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHEECCCCCC
IDSWWQGDKFDRILLDAPCSATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKP
CCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCC
GGTLLYATCSILPQENKDQVSAFLARTPDATLVPIEQQANPDDIGWQIVPGQDNMDGFYY
CCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCEEE
ARLVKD
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]