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The map label for this gene is def [H]

Identifier: 167621968

GI number: 167621968

Start: 31920

End: 32519

Strand: Reverse

Name: def [H]

Synonym: Shal_0027

Alternate gene names: 167621968

Gene position: 32519-31920 (Counterclockwise)

Preceding gene: 167621972

Following gene: 167621967

Centisome position: 0.62

GC content: 42.83

Gene sequence:

>600_bases
ATGCCGCTAACGACTAAAATTGAGCCATTAGCCAAAGTCAGTATAACTAACTGGCTCAGATTTGACAGACTTGTTAAGAG
TTTAGGTATGAGTTTATTAAAAGTTTTACAATTTCCAGACGAAAGATTACGTACTGTAGCAAAGCCGATCGCAGAGTTTA
ACGCTGCTCTTCAAACACAAATCGACGATATGTTCGAAACGATGTACGAAGAGAAGGGAATTGGTCTAGCTTCAACCCAG
GTTAATTACCACCATCAACTTATCGTTATGGATCTTCAAGATGATGTTGAGCGTCCTAAAGTGTTCATCAACTTGGAGAT
TGTTGCCAAAAGTGGTGACTTCTGTAACGAAGAAGGCTGTTTATCTGTTCCTGGCGTCTACGCTAAAGTCGACCGCGCCG
AGTTCGTAACAATCAAAGCCCTCGATCGCGATGGTAATGAGTTCTCTTTAGAGGCTGATGGTTTGTTTGCGATCTGCTTA
CAGCATGAGCTAGACCATCTATCGGGCAAGCTATTTGTTGACTACCTGTCACCACTCAAGCGTCAACGTATCAAACAGAA
GCTAGAAAAAGCCGCTCGTCTCGAAGCCAAACAAGGATAA

Upstream 100 bases:

>100_bases
ACCAACTATAAGTGAAGCAAGTAATATTAACCGTTTCATCAGGGTGTCCATGCTATTTGCTCTTTTATTAGGGTATATAT
AGAGCTTTTGCTGTTATTGA

Downstream 100 bases:

>100_bases
ATTTTTGAAACCATTAAATGTCATTTTTGCAGGAACGCCAGACTTTGCTGCACGCCATCTTCAAGCGCTAATCGATTCAG
AGCATAATGTTATTGCGGTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MPLTTKIEPLAKVSITNWLRFDRLVKSLGMSLLKVLQFPDERLRTVAKPIAEFNAALQTQIDDMFETMYEEKGIGLASTQ
VNYHHQLIVMDLQDDVERPKVFINLEIVAKSGDFCNEEGCLSVPGVYAKVDRAEFVTIKALDRDGNEFSLEADGLFAICL
QHELDHLSGKLFVDYLSPLKRQRIKQKLEKAARLEAKQG

Sequences:

>Translated_199_residues
MPLTTKIEPLAKVSITNWLRFDRLVKSLGMSLLKVLQFPDERLRTVAKPIAEFNAALQTQIDDMFETMYEEKGIGLASTQ
VNYHHQLIVMDLQDDVERPKVFINLEIVAKSGDFCNEEGCLSVPGVYAKVDRAEFVTIKALDRDGNEFSLEADGLFAICL
QHELDHLSGKLFVDYLSPLKRQRIKQKLEKAARLEAKQG
>Mature_198_residues
PLTTKIEPLAKVSITNWLRFDRLVKSLGMSLLKVLQFPDERLRTVAKPIAEFNAALQTQIDDMFETMYEEKGIGLASTQV
NYHHQLIVMDLQDDVERPKVFINLEIVAKSGDFCNEEGCLSVPGVYAKVDRAEFVTIKALDRDGNEFSLEADGLFAICLQ
HELDHLSGKLFVDYLSPLKRQRIKQKLEKAARLEAKQG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=61.9047619047619, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22582; Mature: 22451

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTTKIEPLAKVSITNWLRFDRLVKSLGMSLLKVLQFPDERLRTVAKPIAEFNAALQTQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IDDMFETMYEEKGIGLASTQVNYHHQLIVMDLQDDVERPKVFINLEIVAKSGDFCNEEGC
HHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCCCC
LSVPGVYAKVDRAEFVTIKALDRDGNEFSLEADGLFAICLQHELDHLSGKLFVDYLSPLK
CCCCCHHEECCCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHH
RQRIKQKLEKAARLEAKQG
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLTTKIEPLAKVSITNWLRFDRLVKSLGMSLLKVLQFPDERLRTVAKPIAEFNAALQTQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IDDMFETMYEEKGIGLASTQVNYHHQLIVMDLQDDVERPKVFINLEIVAKSGDFCNEEGC
HHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCCCC
LSVPGVYAKVDRAEFVTIKALDRDGNEFSLEADGLFAICLQHELDHLSGKLFVDYLSPLK
CCCCCHHEECCCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHH
RQRIKQKLEKAARLEAKQG
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]