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The map label for this gene is nifK1

Identifier: 16520018

GI number: 16520018

Start: 136966

End: 138507

Strand: Reverse

Name: nifK1

Synonym: NGR_a01110

Alternate gene names: 16520018

Gene position: 138507-136966 (Counterclockwise)

Preceding gene: 16519993

Following gene: 16519995

Centisome position: 25.83

GC content: 63.62

Gene sequence:

>1542_bases
ATGCCGCAATCGGCTGAGAAGATACTCGACCATGCGCCGCTGTTTCGCGAGCCGGAATACCGGCAGATGCTCGCGGAGAA
GAAGCTGAACTTCGAATGTCCGCACCCCGAACGGCTCGTCACGGACCAGCGCGAATACAGCAAGGGCTGGGAATATCGCG
AGAAAAACCTCGCCCGCGAGGCGCTCGTCGTCAACCCCGCCAAGGCCTGTCAACCGTTGGGGGCGGTGTTCGCAGCCGCC
GGCTTCGAGCGGACGATGTCGTTCGTCCATGGCAGTCAGGGCTGCGTGGCCTATTATCGCTCGCACTTGTCGCGCCACTT
CAAGGAGCCGGCTTCGGCCGTTTCGTCCTCGATGACCGAGGATGCGGCGGTGTTCGGCGGCCTGAAGAACATGGTCGACG
GGCTCGCCAATACCTACGCGCTCTACGATCCGAAGATGATTGCCGTCTCCACCACCTGTATGGCCGAGGTCATCGGCGAC
GACCTGCATGGCTTCATTGAGAACGCCAAGAGCGAAGGCGCAGTCCCGCCCGAATTCGACGTGCCGTTCGCTCACACGCC
CGCCTTCGTCGGCAGCCATGTCGACGGCTATGACAGCATGGTCAAGGGCATCCTGGAGCACTTCTGGAAGGGCCAGGCGC
GCACCCAAGCGGCCGGCACGATCAACATCATCCCGGGCTTCGACGGCTTTTGCGTCGGCAACAACCGCGAGCTTCAGCGC
CTGCTCACCCTGATGGGCGTGTCCTACACCTTCATCCAGGATGCCTCCGACCAGTTCGATACGCCGTCCGACGGCGAATA
CCGCATGTATGACGGGGGCACGACGATCAAGGCGCTGCGGGCGGCACTCAATGCCGAGGCGACGCTGTCGCTGCAGCACT
ACAACAGCCGCAAGACGCTCGAATATTGCCGGGAGGTCGGTCAGGCCACCGCCGCCTTCCATTACCCGCTCGGGATCAAC
GCCACCGACGCGTTCCTGATGAAGGTGTCGGCGATTTCCGGCCGGGAAATCCCCGAGACGATACGCCTGGAACGCGGCCG
GCTGGTCGACGCCATGGCCGACAGCCAATCCTGGCTGCATGGCAAGACATACGCGATCTACGGCGATCCGGACTTCGTCT
ACGCCATGGCCCGCTTCGTCATGGAGACCGGCGGCGAGCCGCGGCATTGCCTCGCCACCAACGGCACGGCGGCCTGGCAG
GCCGAGATGACCGAGCTGCTCGCCTCTTCTCCCTTCGGCAAGCAGGCAAAGGTCTGGCCGGGAAAGGATCTCTGGGCCCT
GCGCTCGCTGCTCTTCACCGAGCCGGTCGACCTGCTGATCGGCAATTCCTACGGCAAGTATCTCGAGCGCGATACCGGCA
CGCCGCTGATCCGGCTGATGTTCCCGATCTTCGACCGCCACCACCACCACCGCTTTCCGCTCATGGGCTACCAGGGCGGC
CTGCGCCTGCTGACGACGATCCTCGACACGATCTTCGACCGCCTCGATCGCGAAACGATGCAGACGGCGGTGACCGATTA
TTCCTATGACCTGACCCGCTAA

Upstream 100 bases:

>100_bases
AATGATCGGCCGGTCTTGCGCGGCACGAGGACCGCGGCAAGACCGGAAACCTCTCGAACATCCCTGGGCCTAAGAGGCCA
GATCAAGAAGGTCAAACACC

Downstream 100 bases:

>100_bases
GAACGGCGGTCGGCAAAACGCCGGCCGTCTCCTGATGCAATCTGGAGATTAGCGAATGTCTTCGCTCAATGTCAAAATCC
AAGATGTCTTCGATGAGCCT

Product: nitrogenase molybdenum-iron protein beta chain NifK1

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MPQSAEKILDHAPLFREPEYRQMLAEKKLNFECPHPERLVTDQREYSKGWEYREKNLAREALVVNPAKACQPLGAVFAAA
GFERTMSFVHGSQGCVAYYRSHLSRHFKEPASAVSSSMTEDAAVFGGLKNMVDGLANTYALYDPKMIAVSTTCMAEVIGD
DLHGFIENAKSEGAVPPEFDVPFAHTPAFVGSHVDGYDSMVKGILEHFWKGQARTQAAGTINIIPGFDGFCVGNNRELQR
LLTLMGVSYTFIQDASDQFDTPSDGEYRMYDGGTTIKALRAALNAEATLSLQHYNSRKTLEYCREVGQATAAFHYPLGIN
ATDAFLMKVSAISGREIPETIRLERGRLVDAMADSQSWLHGKTYAIYGDPDFVYAMARFVMETGGEPRHCLATNGTAAWQ
AEMTELLASSPFGKQAKVWPGKDLWALRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
LRLLTTILDTIFDRLDRETMQTAVTDYSYDLTR

Sequences:

>Translated_513_residues
MPQSAEKILDHAPLFREPEYRQMLAEKKLNFECPHPERLVTDQREYSKGWEYREKNLAREALVVNPAKACQPLGAVFAAA
GFERTMSFVHGSQGCVAYYRSHLSRHFKEPASAVSSSMTEDAAVFGGLKNMVDGLANTYALYDPKMIAVSTTCMAEVIGD
DLHGFIENAKSEGAVPPEFDVPFAHTPAFVGSHVDGYDSMVKGILEHFWKGQARTQAAGTINIIPGFDGFCVGNNRELQR
LLTLMGVSYTFIQDASDQFDTPSDGEYRMYDGGTTIKALRAALNAEATLSLQHYNSRKTLEYCREVGQATAAFHYPLGIN
ATDAFLMKVSAISGREIPETIRLERGRLVDAMADSQSWLHGKTYAIYGDPDFVYAMARFVMETGGEPRHCLATNGTAAWQ
AEMTELLASSPFGKQAKVWPGKDLWALRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
LRLLTTILDTIFDRLDRETMQTAVTDYSYDLTR
>Mature_512_residues
PQSAEKILDHAPLFREPEYRQMLAEKKLNFECPHPERLVTDQREYSKGWEYREKNLAREALVVNPAKACQPLGAVFAAAG
FERTMSFVHGSQGCVAYYRSHLSRHFKEPASAVSSSMTEDAAVFGGLKNMVDGLANTYALYDPKMIAVSTTCMAEVIGDD
LHGFIENAKSEGAVPPEFDVPFAHTPAFVGSHVDGYDSMVKGILEHFWKGQARTQAAGTINIIPGFDGFCVGNNRELQRL
LTLMGVSYTFIQDASDQFDTPSDGEYRMYDGGTTIKALRAALNAEATLSLQHYNSRKTLEYCREVGQATAAFHYPLGINA
TDAFLMKVSAISGREIPETIRLERGRLVDAMADSQSWLHGKTYAIYGDPDFVYAMARFVMETGGEPRHCLATNGTAAWQA
EMTELLASSPFGKQAKVWPGKDLWALRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGGL
RLLTTILDTIFDRLDRETMQTAVTDYSYDLTR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFK_RHISN (P19067)

Other databases:

- EMBL:   U00090
- EMBL:   U00090
- EMBL:   M26963
- PIR:   PS0046
- PIR:   T10830
- RefSeq:   NP_444114.1
- RefSeq:   NP_444138.1
- ProteinModelPortal:   P19067
- SMR:   P19067
- GeneID:   962471
- GeneID:   962485
- GenomeReviews:   U00090_GR
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a00870
- KEGG:   rhi:NGR_a01110
- HOGENOM:   HBG336444
- ProtClustDB:   CLSK864415
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976
- TIGRFAMs:   TIGR01286

Pfam domain/function: PF00148 Oxidored_nitro

EC number: =1.18.6.1

Molecular weight: Translated: 57303; Mature: 57171

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSAEKILDHAPLFREPEYRQMLAEKKLNFECPHPERLVTDQREYSKGWEYREKNLARE
CCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHH
ALVVNPAKACQPLGAVFAAAGFERTMSFVHGSQGCVAYYRSHLSRHFKEPASAVSSSMTE
HHEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAVFGGLKNMVDGLANTYALYDPKMIAVSTTCMAEVIGDDLHGFIENAKSEGAVPPEFD
HHHHHHHHHHHHHHHHHCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VPFAHTPAFVGSHVDGYDSMVKGILEHFWKGQARTQAAGTINIIPGFDGFCVGNNRELQR
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEECCCHHHHH
LLTLMGVSYTFIQDASDQFDTPSDGEYRMYDGGTTIKALRAALNAEATLSLQHYNSRKTL
HHHHHCCHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHH
EYCREVGQATAAFHYPLGINATDAFLMKVSAISGREIPETIRLERGRLVDAMADSQSWLH
HHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHCCCHHHC
GKTYAIYGDPDFVYAMARFVMETGGEPRHCLATNGTAAWQAEMTELLASSPFGKQAKVWP
CCEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
GKDLWALRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
CCHHHHHHHHHHHCCHHHEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
LRLLTTILDTIFDRLDRETMQTAVTDYSYDLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PQSAEKILDHAPLFREPEYRQMLAEKKLNFECPHPERLVTDQREYSKGWEYREKNLARE
CCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHH
ALVVNPAKACQPLGAVFAAAGFERTMSFVHGSQGCVAYYRSHLSRHFKEPASAVSSSMTE
HHEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAVFGGLKNMVDGLANTYALYDPKMIAVSTTCMAEVIGDDLHGFIENAKSEGAVPPEFD
HHHHHHHHHHHHHHHHHCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VPFAHTPAFVGSHVDGYDSMVKGILEHFWKGQARTQAAGTINIIPGFDGFCVGNNRELQR
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEECCCHHHHH
LLTLMGVSYTFIQDASDQFDTPSDGEYRMYDGGTTIKALRAALNAEATLSLQHYNSRKTL
HHHHHCCHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHH
EYCREVGQATAAFHYPLGINATDAFLMKVSAISGREIPETIRLERGRLVDAMADSQSWLH
HHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHCCCHHHC
GKTYAIYGDPDFVYAMARFVMETGGEPRHCLATNGTAAWQAEMTELLASSPFGKQAKVWP
CCEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
GKDLWALRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
CCHHHHHHHHHHHCCHHHEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
LRLLTTILDTIFDRLDRETMQTAVTDYSYDLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485