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The map label for this gene is nifE

Identifier: 16519995

GI number: 16519995

Start: 135420

End: 136910

Strand: Reverse

Name: nifE

Synonym: NGR_a01100

Alternate gene names: 16519995

Gene position: 136910-135420 (Counterclockwise)

Preceding gene: 16520018

Following gene: 16519996

Centisome position: 25.54

GC content: 59.15

Gene sequence:

>1491_bases
ATGTCTTCGCTCAATGTCAAAATCCAAGATGTCTTCGATGAGCCTGCCTGTGAGAGAAATCGCAGCAAGGATTCCAACGC
GCGCAGTAAAGGCTGTTCGAAACCGCTGACCCCCGGCGCGGCGGCCGGAGGCTGCGCCTTCGATGGCGCCAAGATCGTGC
TGCAGCCGATCACCGACGTTGCGCATCTGGTTCATGCACCACTCGCCTGCGAAGGCAATTCCTGGGACAACCGAGGTACG
GCGTCGTCCGGTCCGACGCTTTGGCGCACCAGCTTCACGACCGATCTCACCGAGCAAGACATAGTGATGGGGAACAGCGA
GCGGAAGCTCTTCAAAGCGATCCGCCAGATTAGAGAAGCGTATCAGCCGCCGGCAATCTTTGTCTATGCGACGTGTGTAA
CGGCACTTATCGGCGACGACATCGAAGCGATCTGCAAGCGCGCTGAGGAAACGTGCGGCCTACCGGTGGTGCCGATCAAT
TCGCCGGGCTTCGTCGGTTCGAAGAACCTCGGCAACAAGCTGGCTGGCGAGGCGTTGCTCGACCATGTCATCGGCACGGT
GGAGCCAGATGATGCCGGCCCTTGCGACATCAATATCCTTGGCGAGTTCAACCTGTCTGGTGAGTTCTGGTTGGTAAAGC
CACTCTTGGAGAGGCTTGGCATTCGTGTTCGCGCCTGCATTCCCGGTGACGCCCGCTATCTCGACATTGCCTCCGCTCAT
CGCGCTCGAGCAGCCATGTTGGTGTGCTCTACGGCGTTGATCAACCTTGCCCGCAAAATGGAGGAGCGCTGGGATATCCC
GTTCTTCGAGGGATCCTTCTACGGCATCACCGCCACCTCGGAAGCACTTCGACAGATTGCCGATCTGCTCGTAAAGAAGG
GTACCGATCTAGAGATCCTCGACCGCACCGATGCACTCATTGCGGAGGAGGAGGCGATTGCGTGGAAAAAGCTCGAGGAA
TACCGGCCGCGACTTAAAGGCAAGCGGGTGCTCATCAACACCGGCGGCGTGAAATCCTGGTCGCTTGTTCATGCGCTGAT
GGAGATCGGCATGGAGATCGTTGGCACCTCGGTCAAGAAATCGACGGTCGAGGACAAGGAGCGCATCAGACAGGTGCTTA
AGGACGATCTCCAGATGTTCGAGTCGATGTCGCCCCGTGAGCTCTACGCCATGCTCTCAGAACATAAGGCCGACATCATG
CTGTCGGGCGGGCGCACGCAATTCATTGCGCTGAAGGCCAAGATGCCCTGGGTCGATATCAACCAAGAGCGTCACCGCCC
CTATGCGGGCTATGACGGCACGGTGGAACTCGTGCGCCAGATCGACCTTGCGATTCACAACCCGGTCTGGTCGCAGGTGA
GGGAGCCGGCACCGTGGGAAAGCCGTCCTGCCACACAGGAACCACTGCCGAACACGCGCCCCGACACCGATGCTACGAGT
GTCGGGCGCTCAATTCGCCGGCACGACGCCGGTGACTTCGTTGAGTGTTGA

Upstream 100 bases:

>100_bases
ATGCAGACGGCGGTGACCGATTATTCCTATGACCTGACCCGCTAAGAACGGCGGTCGGCAAAACGCCGGCCGTCTCCTGA
TGCAATCTGGAGATTAGCGA

Downstream 100 bases:

>100_bases
GGAAAGCAAATGGTCCACATCCATCGCCAATCTAAATCAGCGACGGTCAATCCGCTCAAGTCGTCCCAACCGCTGGGTGC
CGCGTTAGCCTTTCTCGGGG

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSSLNVKIQDVFDEPACERNRSKDSNARSKGCSKPLTPGAAAGGCAFDGAKIVLQPITDVAHLVHAPLACEGNSWDNRGT
ASSGPTLWRTSFTTDLTEQDIVMGNSERKLFKAIRQIREAYQPPAIFVYATCVTALIGDDIEAICKRAEETCGLPVVPIN
SPGFVGSKNLGNKLAGEALLDHVIGTVEPDDAGPCDINILGEFNLSGEFWLVKPLLERLGIRVRACIPGDARYLDIASAH
RARAAMLVCSTALINLARKMEERWDIPFFEGSFYGITATSEALRQIADLLVKKGTDLEILDRTDALIAEEEAIAWKKLEE
YRPRLKGKRVLINTGGVKSWSLVHALMEIGMEIVGTSVKKSTVEDKERIRQVLKDDLQMFESMSPRELYAMLSEHKADIM
LSGGRTQFIALKAKMPWVDINQERHRPYAGYDGTVELVRQIDLAIHNPVWSQVREPAPWESRPATQEPLPNTRPDTDATS
VGRSIRRHDAGDFVEC

Sequences:

>Translated_496_residues
MSSLNVKIQDVFDEPACERNRSKDSNARSKGCSKPLTPGAAAGGCAFDGAKIVLQPITDVAHLVHAPLACEGNSWDNRGT
ASSGPTLWRTSFTTDLTEQDIVMGNSERKLFKAIRQIREAYQPPAIFVYATCVTALIGDDIEAICKRAEETCGLPVVPIN
SPGFVGSKNLGNKLAGEALLDHVIGTVEPDDAGPCDINILGEFNLSGEFWLVKPLLERLGIRVRACIPGDARYLDIASAH
RARAAMLVCSTALINLARKMEERWDIPFFEGSFYGITATSEALRQIADLLVKKGTDLEILDRTDALIAEEEAIAWKKLEE
YRPRLKGKRVLINTGGVKSWSLVHALMEIGMEIVGTSVKKSTVEDKERIRQVLKDDLQMFESMSPRELYAMLSEHKADIM
LSGGRTQFIALKAKMPWVDINQERHRPYAGYDGTVELVRQIDLAIHNPVWSQVREPAPWESRPATQEPLPNTRPDTDATS
VGRSIRRHDAGDFVEC
>Mature_495_residues
SSLNVKIQDVFDEPACERNRSKDSNARSKGCSKPLTPGAAAGGCAFDGAKIVLQPITDVAHLVHAPLACEGNSWDNRGTA
SSGPTLWRTSFTTDLTEQDIVMGNSERKLFKAIRQIREAYQPPAIFVYATCVTALIGDDIEAICKRAEETCGLPVVPINS
PGFVGSKNLGNKLAGEALLDHVIGTVEPDDAGPCDINILGEFNLSGEFWLVKPLLERLGIRVRACIPGDARYLDIASAHR
ARAAMLVCSTALINLARKMEERWDIPFFEGSFYGITATSEALRQIADLLVKKGTDLEILDRTDALIAEEEAIAWKKLEEY
RPRLKGKRVLINTGGVKSWSLVHALMEIGMEIVGTSVKKSTVEDKERIRQVLKDDLQMFESMSPRELYAMLSEHKADIML
SGGRTQFIALKAKMPWVDINQERHRPYAGYDGTVELVRQIDLAIHNPVWSQVREPAPWESRPATQEPLPNTRPDTDATSV
GRSIRRHDAGDFVEC

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor)

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFE_RHISN (P55673)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444115.1
- ProteinModelPortal:   P55673
- SMR:   P55673
- GeneID:   962479
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01100
- HOGENOM:   HBG392742
- ProtClustDB:   PRK14478
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973
- TIGRFAMs:   TIGR01283

Pfam domain/function: PF00148 Oxidored_nitro

EC number: NA

Molecular weight: Translated: 54794; Mature: 54663

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLNVKIQDVFDEPACERNRSKDSNARSKGCSKPLTPGAAAGGCAFDGAKIVLQPITDV
CCCCCEEHHHHHCCCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AHLVHAPLACEGNSWDNRGTASSGPTLWRTSFTTDLTEQDIVMGNSERKLFKAIRQIREA
HHHHHCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCCHHEECCCHHHHHHHHHHHHHH
YQPPAIFVYATCVTALIGDDIEAICKRAEETCGLPVVPINSPGFVGSKNLGNKLAGEALL
HCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHH
DHVIGTVEPDDAGPCDINILGEFNLSGEFWLVKPLLERLGIRVRACIPGDARYLDIASAH
HHHHCCCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHCCCEEEEECCCCCCEEEHHHHH
RARAAMLVCSTALINLARKMEERWDIPFFEGSFYGITATSEALRQIADLLVKKGTDLEIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECHHHHHHHHHHHHHHCCCCEEEE
DRTDALIAEEEAIAWKKLEEYRPRLKGKRVLINTGGVKSWSLVHALMEIGMEIVGTSVKK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHH
STVEDKERIRQVLKDDLQMFESMSPRELYAMLSEHKADIMLSGGRTQFIALKAKMPWVDI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCEEEEEEEECCCEEEC
NQERHRPYAGYDGTVELVRQIDLAIHNPVWSQVREPAPWESRPATQEPLPNTRPDTDATS
CHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
VGRSIRRHDAGDFVEC
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSLNVKIQDVFDEPACERNRSKDSNARSKGCSKPLTPGAAAGGCAFDGAKIVLQPITDV
CCCCEEHHHHHCCCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AHLVHAPLACEGNSWDNRGTASSGPTLWRTSFTTDLTEQDIVMGNSERKLFKAIRQIREA
HHHHHCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCCHHEECCCHHHHHHHHHHHHHH
YQPPAIFVYATCVTALIGDDIEAICKRAEETCGLPVVPINSPGFVGSKNLGNKLAGEALL
HCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHH
DHVIGTVEPDDAGPCDINILGEFNLSGEFWLVKPLLERLGIRVRACIPGDARYLDIASAH
HHHHCCCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHCCCEEEEECCCCCCEEEHHHHH
RARAAMLVCSTALINLARKMEERWDIPFFEGSFYGITATSEALRQIADLLVKKGTDLEIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECHHHHHHHHHHHHHHCCCCEEEE
DRTDALIAEEEAIAWKKLEEYRPRLKGKRVLINTGGVKSWSLVHALMEIGMEIVGTSVKK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHH
STVEDKERIRQVLKDDLQMFESMSPRELYAMLSEHKADIMLSGGRTQFIALKAKMPWVDI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCEEEEEEEECCCEEEC
NQERHRPYAGYDGTVELVRQIDLAIHNPVWSQVREPAPWESRPATQEPLPNTRPDTDATS
CHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
VGRSIRRHDAGDFVEC
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424