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The map label for this gene is nifD1

Identifier: 16519993

GI number: 16519993

Start: 138603

End: 140117

Strand: Reverse

Name: nifD1

Synonym: NGR_a01120

Alternate gene names: 16519993

Gene position: 140117-138603 (Counterclockwise)

Preceding gene: 16519992

Following gene: 16520018

Centisome position: 26.13

GC content: 61.72

Gene sequence:

>1515_bases
ATGAGCCTCGATTACGAGAATGACAGTGCGCTCCATCAGGAGCTGATCACGCAAGTGCTGTCGCAGTACCCACACAAGGC
GGCCAAGCGTCGCCAAAAGCACCTCAGTGTCGCATCGGACCGCGAGGCGGTCGGGGAGGAGGGCGAGACCCTCTCCGAAT
GCGACGTGAAGTCGAACATCAAGTCGATCCCCGGGGTGATGACGATCCGCGGCTGCGCCTATGCGGGCTCGAAGGGCGTG
GTCTGGGGCCCGGTCAAGGATATGGTCCACATCTCGCACGGCCCGGTTGGCTGCGGTCAATATTCCTGGTCGCAGCGCCG
CAACTATTATGTCGGCACCACCGGCGTCGACACCTTCGTGACGATGCAGTTCACCTCCGACTTTCAGGAGAAGGACATCG
TCTTTGGTGGCGACAAGAAGCTGGAACAGGTCATCGACGAGATCGAGGAGCTGTTTCCCCTCAACAACGGCATCACCATC
CAGTCCGAATGTCCGATCGGCCTGATTGGCGACGACATCGAAGCGGTGTCGCGCAAGAAGGCCGCCGAACACGAAACGAC
GATCGTGCCGGTGCGCTGCGAAGGCTTCCGCGGCGTCTCGCAGTCGCTCGGCCATCACATCGCCAACGACGCCATCCGCG
ACTGGGTGTTCGACAAGGCGGACGGCAAGACGGACGTCGAGTTCGAAACCGGTCCCTACGATGTCAACGTCATCGGCGAT
TACAACATCGGCGGCGACGCCTGGGCGTCGCGCATCCTGCTCGAGGAGATCGGGCTGCGCGTCGTCGGCAACTGGTCGGG
CGACGCCACGCTCGCGGAAGTGGAGCGGGCCCCCAGGGCCAAGCTCAACCTCATCCACTGCTACCGGTCGATGAACTACA
TCTGCCGGCACATGGAGGAAAGATACGCCATCCCCTGGATGGAATACAACTTCTTCGGCCCCTCCCAGATCGAAGCCTCT
CTGCGCAAGATAGCCAGGCATTTCGGCCCGACGATCGAAGAACGGGCCGAGAGGGTCATCGCCAAGTACCGGCCGCTGGT
CGACGCCGTGATCGACAAGTACTGGCCGCGCCTCCAGGGCAAGCGAGTGATGCTCTATGTCGGTGGTTTGCGCCCGCGCC
ACGTCATCACCGCCTATGAGGACCTCGGCATGCAGATCGTCGGCACCGGCTACGAATTCGCCCACAACGACGACTACCAG
CGCACCGGCCACTACGTGAAGACGGGCACGCTGATCTATGACGACGCGACCAGTTACGAACTGGACACGTTCATCGAGCG
GATCCGCCCCGATCTGGTCGGCTCCGGCATCAAGGAGAAGTATCCGGTGCAGAAGATGGGCATCCCGTTTCGCCAGATGC
ACTCCTGGGATTATTCCGGCCCCTATCACGGCTATGACGGCTTCGCCATCTTCGCCCGCGACATGGATCTCGCCATCAAC
AATCCGGTCTGGGATCTCTACGACGCGCCCTGGAAGAAAATGACCGTGCCGACGGCCGCAGTTGCAGCCGAATGA

Upstream 100 bases:

>100_bases
GTGACCGCCGCCGCAGACGCTGCCCGGGACGGTGATCCGGTGCGACATTCCACCAATGGTGCCTCTTCTTGGAGGACACG
CAAAAAAGGGGGCAGGCCCA

Downstream 100 bases:

>100_bases
TCGGCCGGTCTTGCGCGGCACGAGGACCGCGGCAAGACCGGAAACCTCTCGAACATCCCTGGGCCTAAGAGGCCAGATCA
AGAAGGTCAAACACCATGCC

Product: nitrogenase molybdenum-iron protein alpha chain NifD1

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSLDYENDSALHQELITQVLSQYPHKAAKRRQKHLSVASDREAVGEEGETLSECDVKSNIKSIPGVMTIRGCAYAGSKGV
VWGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGVDTFVTMQFTSDFQEKDIVFGGDKKLEQVIDEIEELFPLNNGITI
QSECPIGLIGDDIEAVSRKKAAEHETTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKADGKTDVEFETGPYDVNVIGD
YNIGGDAWASRILLEEIGLRVVGNWSGDATLAEVERAPRAKLNLIHCYRSMNYICRHMEERYAIPWMEYNFFGPSQIEAS
LRKIARHFGPTIEERAERVIAKYRPLVDAVIDKYWPRLQGKRVMLYVGGLRPRHVITAYEDLGMQIVGTGYEFAHNDDYQ
RTGHYVKTGTLIYDDATSYELDTFIERIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAIN
NPVWDLYDAPWKKMTVPTAAVAAE

Sequences:

>Translated_504_residues
MSLDYENDSALHQELITQVLSQYPHKAAKRRQKHLSVASDREAVGEEGETLSECDVKSNIKSIPGVMTIRGCAYAGSKGV
VWGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGVDTFVTMQFTSDFQEKDIVFGGDKKLEQVIDEIEELFPLNNGITI
QSECPIGLIGDDIEAVSRKKAAEHETTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKADGKTDVEFETGPYDVNVIGD
YNIGGDAWASRILLEEIGLRVVGNWSGDATLAEVERAPRAKLNLIHCYRSMNYICRHMEERYAIPWMEYNFFGPSQIEAS
LRKIARHFGPTIEERAERVIAKYRPLVDAVIDKYWPRLQGKRVMLYVGGLRPRHVITAYEDLGMQIVGTGYEFAHNDDYQ
RTGHYVKTGTLIYDDATSYELDTFIERIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAIN
NPVWDLYDAPWKKMTVPTAAVAAE
>Mature_503_residues
SLDYENDSALHQELITQVLSQYPHKAAKRRQKHLSVASDREAVGEEGETLSECDVKSNIKSIPGVMTIRGCAYAGSKGVV
WGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGVDTFVTMQFTSDFQEKDIVFGGDKKLEQVIDEIEELFPLNNGITIQ
SECPIGLIGDDIEAVSRKKAAEHETTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKADGKTDVEFETGPYDVNVIGDY
NIGGDAWASRILLEEIGLRVVGNWSGDATLAEVERAPRAKLNLIHCYRSMNYICRHMEERYAIPWMEYNFFGPSQIEASL
RKIARHFGPTIEERAERVIAKYRPLVDAVIDKYWPRLQGKRVMLYVGGLRPRHVITAYEDLGMQIVGTGYEFAHNDDYQR
TGHYVKTGTLIYDDATSYELDTFIERIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINN
PVWDLYDAPWKKMTVPTAAVAAE

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFD_RHISN (P19066)

Other databases:

- EMBL:   U00090
- EMBL:   U00090
- EMBL:   M26961
- EMBL:   M26962
- PIR:   PS0045
- PIR:   T10829
- RefSeq:   NP_444113.1
- RefSeq:   NP_444137.1
- ProteinModelPortal:   P19066
- SMR:   P19066
- GeneID:   962482
- GeneID:   962502
- GenomeReviews:   U00090_GR
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a00880
- KEGG:   rhi:NGR_a01120
- HOGENOM:   HBG392742
- ProtClustDB:   CLSK864414
- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972
- TIGRFAMs:   TIGR01862
- TIGRFAMs:   TIGR01282

Pfam domain/function: PF00148 Oxidored_nitro

EC number: =1.18.6.1

Molecular weight: Translated: 56945; Mature: 56814

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDYENDSALHQELITQVLSQYPHKAAKRRQKHLSVASDREAVGEEGETLSECDVKSNI
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHCCCCCCHHHCCHHHHH
KSIPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGVDTFV
HHCCCEEEEEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEE
TMQFTSDFQEKDIVFGGDKKLEQVIDEIEELFPLNNGITIQSECPIGLIGDDIEAVSRKK
EEEECCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHHH
AAEHETTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKADGKTDVEFETGPYDVNVIGD
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEC
YNIGGDAWASRILLEEIGLRVVGNWSGDATLAEVERAPRAKLNLIHCYRSMNYICRHMEE
CCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RYAIPWMEYNFFGPSQIEASLRKIARHFGPTIEERAERVIAKYRPLVDAVIDKYWPRLQG
HCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRVMLYVGGLRPRHVITAYEDLGMQIVGTGYEFAHNDDYQRTGHYVKTGTLIYDDATSYE
CEEEEEECCCCCCCEEEEHHHCCEEEEECCEECCCCCCHHHCCCEEEECEEEEECCCCCC
LDTFIERIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAIN
HHHHHHHHCHHHHCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCCEEEC
NPVWDLYDAPWKKMTVPTAAVAAE
CCCCCCCCCCCCCEECCHHHHCCC
>Mature Secondary Structure 
SLDYENDSALHQELITQVLSQYPHKAAKRRQKHLSVASDREAVGEEGETLSECDVKSNI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHCCCCCCHHHCCHHHHH
KSIPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGVDTFV
HHCCCEEEEEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEE
TMQFTSDFQEKDIVFGGDKKLEQVIDEIEELFPLNNGITIQSECPIGLIGDDIEAVSRKK
EEEECCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHHH
AAEHETTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKADGKTDVEFETGPYDVNVIGD
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEC
YNIGGDAWASRILLEEIGLRVVGNWSGDATLAEVERAPRAKLNLIHCYRSMNYICRHMEE
CCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RYAIPWMEYNFFGPSQIEASLRKIARHFGPTIEERAERVIAKYRPLVDAVIDKYWPRLQG
HCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRVMLYVGGLRPRHVITAYEDLGMQIVGTGYEFAHNDDYQRTGHYVKTGTLIYDDATSYE
CEEEEEECCCCCCCEEEEHHHCCEEEEECCEECCCCCCHHHCCCEEEECEEEEECCCCCC
LDTFIERIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAIN
HHHHHHHHCHHHHCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCCEEEC
NPVWDLYDAPWKKMTVPTAAVAAE
CCCCCCCCCCCCCEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485