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Identifier: 16520001

GI number: 16520001

Start: 128358

End: 129896

Strand: Reverse

Name: Not Available

Synonym: NGR_a01040

Alternate gene names: 16520001

Gene position: 129896-128358 (Counterclockwise)

Preceding gene: 16520000

Following gene: 16520002

Centisome position: 24.23

GC content: 59.52

Gene sequence:

>1539_bases
ATGTCAGAACCGTTCTTTAGGCCGGGGTCGGGGCCCGCGCCCGGCCACCCTACTGCAGAACCTTGGAGAAGAAGGAAGAG
GTGGTACTCGCCAAGCGATGGTCGAGCTGGCACTGCACGGTCGCATTTGCCACAGCGGTCGACGAGCCATGATGGAGTGT
GTTCGCGTATCTGTGCATTTGTCCTATGTATGGTCGCACTGCAATTTCTGATGACCTCAGCAATGGCGGCCGACGAGAGC
CCGCTTCGCGAAGCCGAGGTTGCAAATTTCATGCTGGGCAACGGCATGGAGGTAGTCGTCATACCCGATCACCGGGCGCC
GATCGTCACGCAGATGATCTGGTATAAGGTCGGCAATGCCGACGAGCCACCCGGCAAATCCGGCATTGCTCATTTCCTGG
AGCATCTGATGTTCAAGGGAACGAAGAAGCATCCGTCCGGAGAGTTTAGCGCAAAGATCGCGGAGATCGGCGGTGAGGAG
AACGCCTTCACGGGTTCCGACTACACCGCCTACCATCAGACGGTCACGCCAGAGTCGCTCAGAACAATGATGGAATTCGA
AGCTGACCGGATGCGCCATCTCGTTCTCACCGATGCGGTGATCGTACCCGAACGCGATGTCATTCTTGAAGAGCGACGCT
GGCGCGTCGAGAACGATCCGGAGCAGCTACTTGAGGAGGAGATGCAGGCGACCTTGTATCAGAACCACCCTTACCGCATC
CCGACGATTGGCTGGATGCATGAAATGGAACAGCTTAATCGCGAAGACGCACTAAAGTTCTATGACCGCTACTATGCCCC
CAACAATGCCATTCTGGTCGTGGCAGGCGACGTGGACGCCGGCAGGGTGCGGCAGCTAGCGGACGAGACTTTCGGGACCC
TGCCTCGCGGTCCGGATCTGCCGGCGCGGGTGAGGCCGCAAGAGCCGGAGCAGAACACTAAACGGATCGTCGCGCTCACC
GACCCGCGCGTCACGGTGCCGAGCTTCCAAAAATCCTGGGTAACGACGTCCTACGGAACAGCCGAGCAGGGCGAAGCGGA
AGCCCTCGATATCCTGTCGGAGATTCTCGGCGGGGGAACGCGCAGCCGGATCTATCAGGAGCTGGTGGTCAAACAGGCAA
TAGCCTCCTCTGGCGGGGCCTATTTCAACGGAAGGTCGCTCGATCCGTCGAGCTTCACGGTTTTTGGCTCGCCGCGCGGC
GAGGCGAAGATTGAGGAGGTGGAGGATGCGATAGACGCTGAAATTCGGAAGATCATCGAGTTCGGCATTACCGACGTCGA
GCTCGAGAAGGCCAAAAACCGCTTCGTGCGCTCGATAATCTTCGCGCGCGACAGCCAGTCGGGAATGGCCGGCATTTATG
GGGCGGCGCTTGCGACAGGCGATACTGCACACGACGTAGAGGCGTGGCCACTTAGGATCCGCGCCGTAAAGGCGGCAGAG
GTGCAAGCGGCTGCCAGGAAATACCTCAGTCCCGACCGGTCCGTAGCGGGGTATCTGCTGCCCCGCGAAAGCGCTACCTC
AGGAGATAAAAGCCGATGA

Upstream 100 bases:

>100_bases
AGTTCGATCCAGGTACTTGAATTTAAACGGGTTTTCCGTGGCACGTATCTTGCTCTGCTGACGTGTCAGCGTCGACGAGA
ATTGTGCGGAGAATGTCGAA

Downstream 100 bases:

>100_bases
TATCGTTCAGCCGACTGCGCATCGGGGCCAAGCCCTCACTCGTCGTGGCATGCATATTGCTGGGCCTTGCCGCGTGTCCA
GCGCTTGCGGGCATGGCCAT

Product: Zn-dependent protease, M16 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSEPFFRPGSGPAPGHPTAEPWRRRKRWYSPSDGRAGTARSHLPQRSTSHDGVCSRICAFVLCMVALQFLMTSAMAADES
PLREAEVANFMLGNGMEVVVIPDHRAPIVTQMIWYKVGNADEPPGKSGIAHFLEHLMFKGTKKHPSGEFSAKIAEIGGEE
NAFTGSDYTAYHQTVTPESLRTMMEFEADRMRHLVLTDAVIVPERDVILEERRWRVENDPEQLLEEEMQATLYQNHPYRI
PTIGWMHEMEQLNREDALKFYDRYYAPNNAILVVAGDVDAGRVRQLADETFGTLPRGPDLPARVRPQEPEQNTKRIVALT
DPRVTVPSFQKSWVTTSYGTAEQGEAEALDILSEILGGGTRSRIYQELVVKQAIASSGGAYFNGRSLDPSSFTVFGSPRG
EAKIEEVEDAIDAEIRKIIEFGITDVELEKAKNRFVRSIIFARDSQSGMAGIYGAALATGDTAHDVEAWPLRIRAVKAAE
VQAAARKYLSPDRSVAGYLLPRESATSGDKSR

Sequences:

>Translated_512_residues
MSEPFFRPGSGPAPGHPTAEPWRRRKRWYSPSDGRAGTARSHLPQRSTSHDGVCSRICAFVLCMVALQFLMTSAMAADES
PLREAEVANFMLGNGMEVVVIPDHRAPIVTQMIWYKVGNADEPPGKSGIAHFLEHLMFKGTKKHPSGEFSAKIAEIGGEE
NAFTGSDYTAYHQTVTPESLRTMMEFEADRMRHLVLTDAVIVPERDVILEERRWRVENDPEQLLEEEMQATLYQNHPYRI
PTIGWMHEMEQLNREDALKFYDRYYAPNNAILVVAGDVDAGRVRQLADETFGTLPRGPDLPARVRPQEPEQNTKRIVALT
DPRVTVPSFQKSWVTTSYGTAEQGEAEALDILSEILGGGTRSRIYQELVVKQAIASSGGAYFNGRSLDPSSFTVFGSPRG
EAKIEEVEDAIDAEIRKIIEFGITDVELEKAKNRFVRSIIFARDSQSGMAGIYGAALATGDTAHDVEAWPLRIRAVKAAE
VQAAARKYLSPDRSVAGYLLPRESATSGDKSR
>Mature_511_residues
SEPFFRPGSGPAPGHPTAEPWRRRKRWYSPSDGRAGTARSHLPQRSTSHDGVCSRICAFVLCMVALQFLMTSAMAADESP
LREAEVANFMLGNGMEVVVIPDHRAPIVTQMIWYKVGNADEPPGKSGIAHFLEHLMFKGTKKHPSGEFSAKIAEIGGEEN
AFTGSDYTAYHQTVTPESLRTMMEFEADRMRHLVLTDAVIVPERDVILEERRWRVENDPEQLLEEEMQATLYQNHPYRIP
TIGWMHEMEQLNREDALKFYDRYYAPNNAILVVAGDVDAGRVRQLADETFGTLPRGPDLPARVRPQEPEQNTKRIVALTD
PRVTVPSFQKSWVTTSYGTAEQGEAEALDILSEILGGGTRSRIYQELVVKQAIASSGGAYFNGRSLDPSSFTVFGSPRGE
AKIEEVEDAIDAEIRKIIEFGITDVELEKAKNRFVRSIIFARDSQSGMAGIYGAALATGDTAHDVEAWPLRIRAVKAAEV
QAAARKYLSPDRSVAGYLLPRESATSGDKSR

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family

Homologues:

Organism=Homo sapiens, GI46593007, Length=207, Percent_Identity=29.4685990338164, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI94538354, Length=222, Percent_Identity=29.2792792792793, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI155969707, Length=272, Percent_Identity=26.8382352941176, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1787770, Length=234, Percent_Identity=28.2051282051282, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI2367164, Length=212, Percent_Identity=26.4150943396226, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71999683, Length=200, Percent_Identity=25, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17553678, Length=441, Percent_Identity=24.7165532879819, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994338, Length=90, Percent_Identity=40, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25146563, Length=90, Percent_Identity=40, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25146566, Length=90, Percent_Identity=40, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32566665, Length=90, Percent_Identity=40, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17557500, Length=95, Percent_Identity=36.8421052631579, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323192, Length=389, Percent_Identity=24.4215938303342, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323421, Length=259, Percent_Identity=23.5521235521236, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21357875, Length=215, Percent_Identity=28.8372093023256, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24646943, Length=215, Percent_Identity=28.8372093023256, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI221513245, Length=290, Percent_Identity=22.7586206896552, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4WA_RHISN (P55679)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444121.1
- ProteinModelPortal:   P55679
- SMR:   P55679
- GeneID:   962487
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01040
- HOGENOM:   HBG634529
- ProtClustDB:   CLSK785110
- GO:   GO:0006508
- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863
- Gene3D:   G3DSA:3.30.830.10

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C; SSF63411 Metalloenz_metal-bd

EC number: 3.4.99.- [C]

Molecular weight: Translated: 56887; Mature: 56756

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00143 INSULINASE

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

HASH(0xbd0d00c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPFFRPGSGPAPGHPTAEPWRRRKRWYSPSDGRAGTARSHLPQRSTSHDGVCSRICAF
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
VLCMVALQFLMTSAMAADESPLREAEVANFMLGNGMEVVVIPDHRAPIVTQMIWYKVGNA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCC
DEPPGKSGIAHFLEHLMFKGTKKHPSGEFSAKIAEIGGEENAFTGSDYTAYHQTVTPESL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEHCCCCCHHHH
RTMMEFEADRMRHLVLTDAVIVPERDVILEERRWRVENDPEQLLEEEMQATLYQNHPYRI
HHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEC
PTIGWMHEMEQLNREDALKFYDRYYAPNNAILVVAGDVDAGRVRQLADETFGTLPRGPDL
CCHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
PARVRPQEPEQNTKRIVALTDPRVTVPSFQKSWVTTSYGTAEQGEAEALDILSEILGGGT
CCCCCCCCCCCCCCEEEEECCCCEECCCCHHCCEECCCCCCCCCHHHHHHHHHHHHCCCH
RSRIYQELVVKQAIASSGGAYFNGRSLDPSSFTVFGSPRGEAKIEEVEDAIDAEIRKIIE
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
FGITDVELEKAKNRFVRSIIFARDSQSGMAGIYGAALATGDTAHDVEAWPLRIRAVKAAE
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHH
VQAAARKYLSPDRSVAGYLLPRESATSGDKSR
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCH
>Mature Secondary Structure 
SEPFFRPGSGPAPGHPTAEPWRRRKRWYSPSDGRAGTARSHLPQRSTSHDGVCSRICAF
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
VLCMVALQFLMTSAMAADESPLREAEVANFMLGNGMEVVVIPDHRAPIVTQMIWYKVGNA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCC
DEPPGKSGIAHFLEHLMFKGTKKHPSGEFSAKIAEIGGEENAFTGSDYTAYHQTVTPESL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEHCCCCCHHHH
RTMMEFEADRMRHLVLTDAVIVPERDVILEERRWRVENDPEQLLEEEMQATLYQNHPYRI
HHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEC
PTIGWMHEMEQLNREDALKFYDRYYAPNNAILVVAGDVDAGRVRQLADETFGTLPRGPDL
CCHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
PARVRPQEPEQNTKRIVALTDPRVTVPSFQKSWVTTSYGTAEQGEAEALDILSEILGGGT
CCCCCCCCCCCCCCEEEEECCCCEECCCCHHCCEECCCCCCCCCHHHHHHHHHHHHCCCH
RSRIYQELVVKQAIASSGGAYFNGRSLDPSSFTVFGSPRGEAKIEEVEDAIDAEIRKIIE
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
FGITDVELEKAKNRFVRSIIFARDSQSGMAGIYGAALATGDTAHDVEAWPLRIRAVKAAE
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHH
VQAAARKYLSPDRSVAGYLLPRESATSGDKSR
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424