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The map label for this gene is nifH

Identifier: 16519992

GI number: 16519992

Start: 140214

End: 141104

Strand: Reverse

Name: nifH

Synonym: NGR_a01130

Alternate gene names: 16519992

Gene position: 141104-140214 (Counterclockwise)

Preceding gene: 16519991

Following gene: 16519993

Centisome position: 26.32

GC content: 63.52

Gene sequence:

>891_bases
ATGGCAGGTCTGCGTCAAATCGCGTTTTACGGCAAGGGCGGTATCGGCAAGTCGACCACCTCGCAGAACACGCTCGCCGC
CCTTGTCGACCTCGGGCAGAAGATCCTCATCGTCGGCTGCGATCCCAAGGCCGACTCCACCCGGCTCATCCTCAACGCGA
AGGCGCAGGACACGGTCCTGCATCTGGCGGCAAAGGAGGGATCGGTGGAAGACCTCGAGGTCGAGGACGTGCTCAAGGTC
GGCTACAAGGGCATCAAATGCGTCGAGTCCGGCGGCCCCGAACCGGGTGTCGGCTGCGCCGGCCGCGGCGTCATCACCTC
GATCAACTTCCTGGAGGAAAATGGCGCCTATGACGATGTCGACTACGTCTCCTACGACGTGCTGGGCGACGTGGTGTGCG
GCGGCTTCGCGATGCCGATCCGCGAGAACAAGGCGCAGGAAATCTACATCGTCATGTCCGGCGAGATGATGGCGCTCTAT
GCCGCCAACAACATCGCCAAGGGGATCCTCAAATACGCCCATTCGGGCGGCGTGCGGCTCGGCGGGCTGATTTGCAACGA
GCGCCAGACGGACCGCGAGCTCGATCTCGCCGAGGCGCTGGCGGCCAAGCTCAATTCCAGGCTCATCCACTTCGTGCCGC
GCGACAACATCGTCCAGCACGCCGAGCTCAGGAAGATGACGGTGATCCAGTATGCCCCGGAGTCGCAACAGGCGGCGGAG
TATCGCGCGCTGGCCGACAAGATCCATGCCAATTCCGGCCAGGGCACCGTCCCGACCCCGATCACCATGGAGGAGCTGGA
GGACATGCTGCTCGATTTCGGCGTCATGAAGACCGACGAGCAGATGCTTGCCGAACTTCAGGCCAAGGAAGCGGCGGCAG
CGGCCCAGTGA

Upstream 100 bases:

>100_bases
GCACGGCTTTTGAAGCCTTCCTTGTGCAGGCGGCTTGAAGCTGCCGCTGTATTCGTGTTGCGGGCAACCGCGATGGTTTC
GAACAACGAAGGAAAGCAAC

Downstream 100 bases:

>100_bases
CCGCCGCCGCAGACGCTGCCCGGGACGGTGATCCGGTGCGACATTCCACCAATGGTGCCTCTTCTTGGAGGACACGCAAA
AAAGGGGGCAGGCCCAATGA

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MAGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVLHLAAKEGSVEDLEVEDVLKV
GYKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYAHSGGVRLGGLICNERQTDRELDLAEALAAKLNSRLIHFVPRDNIVQHAELRKMTVIQYAPESQQAAE
YRALADKIHANSGQGTVPTPITMEELEDMLLDFGVMKTDEQMLAELQAKEAAAAAQ

Sequences:

>Translated_296_residues
MAGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVLHLAAKEGSVEDLEVEDVLKV
GYKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYAHSGGVRLGGLICNERQTDRELDLAEALAAKLNSRLIHFVPRDNIVQHAELRKMTVIQYAPESQQAAE
YRALADKIHANSGQGTVPTPITMEELEDMLLDFGVMKTDEQMLAELQAKEAAAAAQ
>Mature_295_residues
AGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVLHLAAKEGSVEDLEVEDVLKVG
YKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYA
ANNIAKGILKYAHSGGVRLGGLICNERQTDRELDLAEALAAKLNSRLIHFVPRDNIVQHAELRKMTVIQYAPESQQAAEY
RALADKIHANSGQGTVPTPITMEELEDMLLDFGVMKTDEQMLAELQAKEAAAAAQ

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFH_RHISN (P19068)

Other databases:

- EMBL:   M26961
- EMBL:   U00090
- EMBL:   U00090
- PIR:   JS0238
- RefSeq:   NP_444112.1
- RefSeq:   NP_444136.1
- ProteinModelPortal:   P19068
- SMR:   P19068
- GeneID:   962478
- GeneID:   962500
- GenomeReviews:   U00090_GR
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a00890
- KEGG:   rhi:NGR_a01130
- HOGENOM:   HBG488165
- ProtClustDB:   PRK13234
- HAMAP:   MF_00533
- InterPro:   IPR000392
- InterPro:   IPR005977
- PIRSF:   PIRSF000363
- PRINTS:   PR00091
- TIGRFAMs:   TIGR01287

Pfam domain/function: PF00142 Fer4_NifH

EC number: =1.18.6.1

Molecular weight: Translated: 31748; Mature: 31616

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00746 NIFH_FRXC_1; PS00692 NIFH_FRXC_2; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVL
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCEEE
HLAAKEGSVEDLEVEDVLKVGYKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDV
EEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCH
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHCCCCEE
GGLICNERQTDRELDLAEALAAKLNSRLIHFVPRDNIVQHAELRKMTVIQYAPESQQAAE
EEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHEEEEEECCCHHHHHH
YRALADKIHANSGQGTVPTPITMEELEDMLLDFGVMKTDEQMLAELQAKEAAAAAQ
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVL
CCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCEEE
HLAAKEGSVEDLEVEDVLKVGYKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDV
EEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCH
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHCCCCEE
GGLICNERQTDRELDLAEALAAKLNSRLIHFVPRDNIVQHAELRKMTVIQYAPESQQAAE
EEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHEEEEEECCCHHHHHH
YRALADKIHANSGQGTVPTPITMEELEDMLLDFGVMKTDEQMLAELQAKEAAAAAQ
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2744485; 9163424