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The map label for this gene is fixC

Identifier: 16519981

GI number: 16519981

Start: 153185

End: 154492

Strand: Direct

Name: fixC

Synonym: NGR_a01240

Alternate gene names: 16519981

Gene position: 153185-154492 (Clockwise)

Preceding gene: 16519982

Following gene: 16519980

Centisome position: 28.57

GC content: 58.03

Gene sequence:

>1308_bases
ATGACCGAAGAAAGATTCGACGCCATCGTCGTCGGTGCCGGTATGTCTGGAAACGCGGCTGCATACACCATGGCAAGTCG
CGGTCTAAAAGTGTTGCAGTTGGAGCGCGGAGAATATCCGGGCTCCAAGAACGTCCAAGGCGCCATCTTGTACGCCAACA
TGCTGGAGACAATCATTCCCGATTTCCGGGATGATGCACCTCTTGAGCGGCATCTGGTCGAGCAGCGATTCTGGGTAACG
GACGACACGTCACACACCGGAATGCACTACCGGTCGGACGACTTCAATGAGCCGGCGCCAAACCGTTACACGATCATTCG
CGCCCAGTTCGACAAGTGGTTTTCGCGGAAAGTCCGAGCGGCGGGCGCGATAGTTTTGTGCGAGACGACGGTGACGGAAC
TGGCTCTTGATGCTGCCGGCAAGGTGATAGGCGTCCATACCGATCGAGCGGGCGGGGTCATCCATGCGGACGTGGTCGTG
CTCGCCGAAGGCGTAAACGGCCTCCTCGGCACGAGAGCACGATTTCGCAAGGTGCCGAAACCCGAAGCCGTGGCACTCGC
TGTCAAGGAAATGCATTTCCTACCGGAAGAGGTCATCAATGAACGCTTCGGCCTTACCGGCGATCAAGGCTGTGTTATTG
AGGCGGCAGGGACAATCTCGCGCGGAATGGCCGGCTTGGGATTCCTCTACACCAACAAGGAATCGATCTCTCTTGGCATC
GGCTGCCTTGTCTCCCATTTCGCAGCGACCCTGGAAAGTCCTTACGCGCTACTTGACGCATTCAAGAACCACCCATCAAT
CCGGCCGCTACTTGCGGGCTCGGAGATTAAGGAATATGCGGCGCACCTCATTCCGGAGGGCGGATTCAAGGCAATTCCGC
AGCTCTTTGGCGATGGCTGGGTCGTGGTTGGCGACGCGGCGCAGCTCAACAACGCCGTGCACCGCGAGGGCTCGAACCTC
GCCATGACGTCGGGCCGCATCGCCGGCGAGGCGATCGCCGCAATCAAGAGCCGAAAACACCCGATGACGAGTGACAATCT
CTCACTTTACAAGGCCATGCTGGACAAGTCTTTCGTTCTGAAAGACCTCAGAAAGTACAAGGACATGCCCGCTCTGCTCC
ACACGAATTCACACAATTTCTTCACGACTTATCCGCAGTTGATGTCGCAGGCGGCGCAAAACTTCGTGCGCGTCGACGGC
ACACCCAAGATCGAGAAGGAAAGAGCGACCACGGCCGCTCTTATTAAAGCGCGTTCGCGATGGGGACTGGTCAGCGACGC
AGTCCGCTTGGCACTGGCATGGCGCTAA

Upstream 100 bases:

>100_bases
GTCACCGATGCTGTCAGCTTTCTGCCGGCGTTGACGGAAGCTTTCACCAAACGGTTGGCGCCGGCCAATCGTCTGAGAGT
TGTGAACTGAGGTAAAACCT

Downstream 100 bases:

>100_bases
AGGGGAGCATTGAGATGACAGCTTCAGTCGTTCGCATTGAAGACAAGCTTTACCAAAACCGTTACCTGGTGGACGCGGGG
CGGCCGCACATCAAGGTGCG

Product: nitrogen fixation protein FixC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTEERFDAIVVGAGMSGNAAAYTMASRGLKVLQLERGEYPGSKNVQGAILYANMLETIIPDFRDDAPLERHLVEQRFWVT
DDTSHTGMHYRSDDFNEPAPNRYTIIRAQFDKWFSRKVRAAGAIVLCETTVTELALDAAGKVIGVHTDRAGGVIHADVVV
LAEGVNGLLGTRARFRKVPKPEAVALAVKEMHFLPEEVINERFGLTGDQGCVIEAAGTISRGMAGLGFLYTNKESISLGI
GCLVSHFAATLESPYALLDAFKNHPSIRPLLAGSEIKEYAAHLIPEGGFKAIPQLFGDGWVVVGDAAQLNNAVHREGSNL
AMTSGRIAGEAIAAIKSRKHPMTSDNLSLYKAMLDKSFVLKDLRKYKDMPALLHTNSHNFFTTYPQLMSQAAQNFVRVDG
TPKIEKERATTAALIKARSRWGLVSDAVRLALAWR

Sequences:

>Translated_435_residues
MTEERFDAIVVGAGMSGNAAAYTMASRGLKVLQLERGEYPGSKNVQGAILYANMLETIIPDFRDDAPLERHLVEQRFWVT
DDTSHTGMHYRSDDFNEPAPNRYTIIRAQFDKWFSRKVRAAGAIVLCETTVTELALDAAGKVIGVHTDRAGGVIHADVVV
LAEGVNGLLGTRARFRKVPKPEAVALAVKEMHFLPEEVINERFGLTGDQGCVIEAAGTISRGMAGLGFLYTNKESISLGI
GCLVSHFAATLESPYALLDAFKNHPSIRPLLAGSEIKEYAAHLIPEGGFKAIPQLFGDGWVVVGDAAQLNNAVHREGSNL
AMTSGRIAGEAIAAIKSRKHPMTSDNLSLYKAMLDKSFVLKDLRKYKDMPALLHTNSHNFFTTYPQLMSQAAQNFVRVDG
TPKIEKERATTAALIKARSRWGLVSDAVRLALAWR
>Mature_434_residues
TEERFDAIVVGAGMSGNAAAYTMASRGLKVLQLERGEYPGSKNVQGAILYANMLETIIPDFRDDAPLERHLVEQRFWVTD
DTSHTGMHYRSDDFNEPAPNRYTIIRAQFDKWFSRKVRAAGAIVLCETTVTELALDAAGKVIGVHTDRAGGVIHADVVVL
AEGVNGLLGTRARFRKVPKPEAVALAVKEMHFLPEEVINERFGLTGDQGCVIEAAGTISRGMAGLGFLYTNKESISLGIG
CLVSHFAATLESPYALLDAFKNHPSIRPLLAGSEIKEYAAHLIPEGGFKAIPQLFGDGWVVVGDAAQLNNAVHREGSNLA
MTSGRIAGEAIAAIKSRKHPMTSDNLSLYKAMLDKSFVLKDLRKYKDMPALLHTNSHNFFTTYPQLMSQAAQNFVRVDGT
PKIEKERATTAALIKARSRWGLVSDAVRLALAWR

Specific function: Could be required for the formation of a functional nitrogenase Fe protein. Probably accepts electrons from fixA/fixB and reduces a quinone

COG id: COG0644

COG function: function code C; Dehydrogenases (flavoproteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF-QO/fixC family

Homologues:

Organism=Homo sapiens, GI119703746, Length=422, Percent_Identity=25.5924170616114, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786227, Length=412, Percent_Identity=38.3495145631068, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1787991, Length=405, Percent_Identity=37.5308641975309, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87082155, Length=416, Percent_Identity=32.4519230769231, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI25144720, Length=400, Percent_Identity=23, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324932, Length=362, Percent_Identity=27.9005524861878, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24652256, Length=418, Percent_Identity=25.5980861244019, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXC_RHISN (Q53208)

Other databases:

- EMBL:   Z68203
- EMBL:   U00090
- RefSeq:   NP_444101.1
- ProteinModelPortal:   Q53208
- GeneID:   962191
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01240
- HOGENOM:   HBG295204
- ProtClustDB:   CLSK2299088
- InterPro:   IPR002218

Pfam domain/function: PF01134 GIDA

EC number: 1.5.5.-

Molecular weight: Translated: 47537; Mature: 47406

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEERFDAIVVGAGMSGNAAAYTMASRGLKVLQLERGEYPGSKNVQGAILYANMLETIIP
CCCCCEEEEEEECCCCCCCCEEHHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHCC
DFRDDAPLERHLVEQRFWVTDDTSHTGMHYRSDDFNEPAPNRYTIIRAQFDKWFSRKVRA
CCCCCCCHHHHHHHHHCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHH
AGAIVLCETTVTELALDAAGKVIGVHTDRAGGVIHADVVVLAEGVNGLLGTRARFRKVPK
CCEEEEEEHHHHHHHHHHCCCEEEEEECCCCCEEEEHHEEEECCCCHHHCCHHHHHCCCC
PEAVALAVKEMHFLPEEVINERFGLTGDQGCVIEAAGTISRGMAGLGFLYTNKESISLGI
CHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCHHHHCCCCCEEEEECCCHHHHHH
GCLVSHFAATLESPYALLDAFKNHPSIRPLLAGSEIKEYAAHLIPEGGFKAIPQLFGDGW
HHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHCCCE
VVVGDAAQLNNAVHREGSNLAMTSGRIAGEAIAAIKSRKHPMTSDNLSLYKAMLDKSFVL
EEECCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KDLRKYKDMPALLHTNSHNFFTTYPQLMSQAAQNFVRVDGTPKIEKERATTAALIKARSR
HHHHHHCCCCCEEEECCCCEEECHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHH
WGLVSDAVRLALAWR
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
TEERFDAIVVGAGMSGNAAAYTMASRGLKVLQLERGEYPGSKNVQGAILYANMLETIIP
CCCCEEEEEEECCCCCCCCEEHHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHCC
DFRDDAPLERHLVEQRFWVTDDTSHTGMHYRSDDFNEPAPNRYTIIRAQFDKWFSRKVRA
CCCCCCCHHHHHHHHHCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHH
AGAIVLCETTVTELALDAAGKVIGVHTDRAGGVIHADVVVLAEGVNGLLGTRARFRKVPK
CCEEEEEEHHHHHHHHHHCCCEEEEEECCCCCEEEEHHEEEECCCCHHHCCHHHHHCCCC
PEAVALAVKEMHFLPEEVINERFGLTGDQGCVIEAAGTISRGMAGLGFLYTNKESISLGI
CHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCHHHHCCCCCEEEEECCCHHHHHH
GCLVSHFAATLESPYALLDAFKNHPSIRPLLAGSEIKEYAAHLIPEGGFKAIPQLFGDGW
HHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHCCCE
VVVGDAAQLNNAVHREGSNLAMTSGRIAGEAIAAIKSRKHPMTSDNLSLYKAMLDKSFVL
EEECCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KDLRKYKDMPALLHTNSHNFFTTYPQLMSQAAQNFVRVDGTPKIEKERATTAALIKARSR
HHHHHHCCCCCEEEECCCCEEECHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHH
WGLVSDAVRLALAWR
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424