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The map label for this gene is fixX

Identifier: 16519980

GI number: 16519980

Start: 154507

End: 154800

Strand: Direct

Name: fixX

Synonym: NGR_a01250

Alternate gene names: 16519980

Gene position: 154507-154800 (Clockwise)

Preceding gene: 16519981

Following gene: 16519979

Centisome position: 28.82

GC content: 53.4

Gene sequence:

>294_bases
ATGACAGCTTCAGTCGTTCGCATTGAAGACAAGCTTTACCAAAACCGTTACCTGGTGGACGCGGGGCGGCCGCACATCAA
GGTGCGGCAGCATCAGTCTCCGAGCCCAAACCTGCTTGCGCTGACCGTTGTTTGCCCAGCGAAATGCTACGAGGTGAATG
AAGACGGCCAAGTAGAGGTTATTGCGGATGGATGCATGGAATGCGGGACATGTAGAGTACTGTGCGAAGAGAGCGGTGAC
ATTGATTGGAGCTATCCGCGAGGTGGATTCGGTGTGCTCTTTAAATTCGGATGA

Upstream 100 bases:

>100_bases
GAGCGACCACGGCCGCTCTTATTAAAGCGCGTTCGCGATGGGGACTGGTCAGCGACGCAGTCCGCTTGGCACTGGCATGG
CGCTAAAGGGGAGCATTGAG

Downstream 100 bases:

>100_bases
CTTGCATCTCACTTTTGTCTAACAAGATGGGTACGTTGACGGGCGCTCACAGGTGCAAGCGCAAGCACTTGATAATGTCA
GCAAGGCGGCATTTTCTCGT

Product: nitrogen fixation protein FixX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 97; Mature: 96

Protein sequence:

>97_residues
MTASVVRIEDKLYQNRYLVDAGRPHIKVRQHQSPSPNLLALTVVCPAKCYEVNEDGQVEVIADGCMECGTCRVLCEESGD
IDWSYPRGGFGVLFKFG

Sequences:

>Translated_97_residues
MTASVVRIEDKLYQNRYLVDAGRPHIKVRQHQSPSPNLLALTVVCPAKCYEVNEDGQVEVIADGCMECGTCRVLCEESGD
IDWSYPRGGFGVLFKFG
>Mature_96_residues
TASVVRIEDKLYQNRYLVDAGRPHIKVRQHQSPSPNLLALTVVCPAKCYEVNEDGQVEVIADGCMECGTCRVLCEESGDI
DWSYPRGGFGVLFKFG

Specific function: Could be a 3Fe-4S cluster-containing protein

COG id: COG2440

COG function: function code C; Ferredoxin-like protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To ferredoxins from P.putida and C.tartarivorum, ferredoxin I from A.vinelandii, ferredoxin II from D.desulfuricans

Homologues:

Organism=Escherichia coli, GI1787992, Length=95, Percent_Identity=38.9473684210526, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1786228, Length=95, Percent_Identity=42.1052631578947, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI87082156, Length=57, Percent_Identity=42.1052631578947, Blast_Score=62, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXX_RHISN (Q53207)

Other databases:

- EMBL:   Z68203
- EMBL:   U00090
- RefSeq:   NP_444100.1
- GeneID:   962468
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01250
- HOGENOM:   HBG498683
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012206
- PIRSF:   PIRSF036548

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 10781; Mature: 10650

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASVVRIEDKLYQNRYLVDAGRPHIKVRQHQSPSPNLLALTVVCPAKCYEVNEDGQVEV
CCCEEEEECCHHCCCEEEEECCCCCEEEECCCCCCCCEEEEEEECCCEEEEECCCCCEEE
IADGCMECGTCRVLCEESGDIDWSYPRGGFGVLFKFG
EECCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
TASVVRIEDKLYQNRYLVDAGRPHIKVRQHQSPSPNLLALTVVCPAKCYEVNEDGQVEV
CCEEEEECCHHCCCEEEEECCCCCEEEECCCCCCCCEEEEEEECCCEEEEECCCCCEEE
IADGCMECGTCRVLCEESGDIDWSYPRGGFGVLFKFG
EECCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424