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The map label for this gene is nifA

Identifier: 16519979

GI number: 16519979

Start: 154853

End: 156637

Strand: Direct

Name: nifA

Synonym: NGR_a01260

Alternate gene names: 16519979

Gene position: 154853-156637 (Clockwise)

Preceding gene: 16519980

Following gene: 16519978

Centisome position: 28.88

GC content: 56.3

Gene sequence:

>1785_bases
GTGCAAGCGCAAGCACTTGATAATGTCAGCAAGGCGGCATTTTCTCGTAGGGAGCCAAGGGCCGTTCGTACGACGCTCAA
GGTGTTTCCTCGTAAGAATTGGCTCGATGAGTGTATGACTGAATTATCCCCTAAAACCATGCACAAACGGGACTTGGGGT
GCAGCGGTCTCTATCGGATATCGAAGGTTTTGATCACTTCAGCCAGCCTCGAGATCAAGCTTGCCAATGTGATTAACACC
CTCCCCGCACTTTTGCCAATGCGGCGCGGCGCAATCGTCGTTGTTGGCGCTGAAGGAGAGCCCGAGACAACTGCGACGTT
CGGGGTTGAGCCTCCATCATCAGGCGCGCGCCACATTGCTGCGAAGGCTGCAATAGATAGAATCGTCGCAAAAGGTGCGC
CGCTGGTCGTACCGGACACTTGCAAGTCGGAACTGTTCCAGGATGAGCTTCAGAGTATCGTGAGCGGCACTGGCCAAGTC
ACCTTCATTGGTGTCCCGATGAAGGCCGATCAAGAAACGCTTGGAACATTATGGATCGACCGCGCCAAGGATGGCGCAGC
CACCAGAACACAATTTGAGGAAGAGGTGCGTTTCCTGTCGATGGTCGCCAACCTTGCGGCACGAGCGGTTCGGCTGAATG
GCCACGAGAGCCGCGATAGTCGACCGATCGGCGAGGAGGGCGATCGCAAGATTAGTGGAGGCGACAAGGAACTGCCTGAA
CCAACCCGACAACGGCCTACGAGAATCGACTGGATTGTCGGGGAAAGCCCCGCACTCAGGCAAGTGGTTGAAAGCGTCAA
AGTCGTTGCACAAACCAACTCTGCGGTGCTTCTCAGGGGCGAAAGCGGCACAGGCAAGGAATTCTTTGCAAAGGCAATCC
ACGAGCTTTCACCTCGCAGAAAAAAGCCGTTTGTGAAGTTGAATTGCGCGGCACTGTCTGCAGGCGTCCTGGAATCGGAA
CTGTTTGGGCATGAGAAGGGCGCCTTTACGGGGGCCATCTCTCAGCGCGCAGGCCGTTTCGAACTGGCGGATGGCGGGAC
GCTGTTGCTCGACGAAATCGGCGAGATCTCGCCGGCCTTTCAAGCGAAACTGTTGCGCGTCTTGCAGGAAGGTGAGCTTG
AGCGAGTTGGCGGCACAAAAACGCTCAAGGTGGACGTCCGACTCATATGCGCCACCAACAAGGACCTGGAGACGGCGGTG
GCGGAGGGGGAGTTCCGGGCTGACCTTTATTATCGCATCAATGTAGTGCCTCTCTTTTTACCGCCTCTCAGGGAGCGAAA
TGGAGATATTCCACGCCTTGCGAAGGTTTTCCTCGACCGGTTCAACAGGGAAAACAATCGCAATCTCGAATTCACGCCTG
CGGCACTCGAGATCCTGTCAAGATGCAAATTTCCCGGCAACGTCCGAGAGCTTGAAAACTGCGTCCGCAGGACCGCCACT
CTCGCACGTTCGGCGACGATTGTTCCATCAGATTTCTCCTGCCAGAATGACCAGTGCTTTTCTTCAATGCTCGGGAAAAC
TGCTGACAGACCGCTTGGCAGCCATTCGCTCAATGGATTGGCCATGAGCAAACGTTTGCCAGTCGAATCGCCGGCCAGTC
TCGGTTACCCCGCCGGCCCTGGCGGCTTAACGGTGGCACCACATCTATCGGACCGCGAGCTGCTAATCAGCGCGATGGAG
AAGGCCGGCTGGGTTCAGGCGAAGGCAGCTCGGATCCTCGGCCTCACACCGCGGCAAGTCGGCTATGCTTTACGGCGGCA
TCATATACAGGTGAAAAAAATCTAG

Upstream 100 bases:

>100_bases
TGGAGCTATCCGCGAGGTGGATTCGGTGTGCTCTTTAAATTCGGATGACTTGCATCTCACTTTTGTCTAACAAGATGGGT
ACGTTGACGGGCGCTCACAG

Downstream 100 bases:

>100_bases
AGACGGTGAGCCCTCTTATCTGGGCTCGGCCATTCCCCTCCTCCTCAGGCGCGCGGGTGTATCGTCCAATTTGGCCGCTT
CTGTCGCGAATTCGACAAAA

Product: nitrogen fixation protein NifA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MQAQALDNVSKAAFSRREPRAVRTTLKVFPRKNWLDECMTELSPKTMHKRDLGCSGLYRISKVLITSASLEIKLANVINT
LPALLPMRRGAIVVVGAEGEPETTATFGVEPPSSGARHIAAKAAIDRIVAKGAPLVVPDTCKSELFQDELQSIVSGTGQV
TFIGVPMKADQETLGTLWIDRAKDGAATRTQFEEEVRFLSMVANLAARAVRLNGHESRDSRPIGEEGDRKISGGDKELPE
PTRQRPTRIDWIVGESPALRQVVESVKVVAQTNSAVLLRGESGTGKEFFAKAIHELSPRRKKPFVKLNCAALSAGVLESE
LFGHEKGAFTGAISQRAGRFELADGGTLLLDEIGEISPAFQAKLLRVLQEGELERVGGTKTLKVDVRLICATNKDLETAV
AEGEFRADLYYRINVVPLFLPPLRERNGDIPRLAKVFLDRFNRENNRNLEFTPAALEILSRCKFPGNVRELENCVRRTAT
LARSATIVPSDFSCQNDQCFSSMLGKTADRPLGSHSLNGLAMSKRLPVESPASLGYPAGPGGLTVAPHLSDRELLISAME
KAGWVQAKAARILGLTPRQVGYALRRHHIQVKKI

Sequences:

>Translated_594_residues
MQAQALDNVSKAAFSRREPRAVRTTLKVFPRKNWLDECMTELSPKTMHKRDLGCSGLYRISKVLITSASLEIKLANVINT
LPALLPMRRGAIVVVGAEGEPETTATFGVEPPSSGARHIAAKAAIDRIVAKGAPLVVPDTCKSELFQDELQSIVSGTGQV
TFIGVPMKADQETLGTLWIDRAKDGAATRTQFEEEVRFLSMVANLAARAVRLNGHESRDSRPIGEEGDRKISGGDKELPE
PTRQRPTRIDWIVGESPALRQVVESVKVVAQTNSAVLLRGESGTGKEFFAKAIHELSPRRKKPFVKLNCAALSAGVLESE
LFGHEKGAFTGAISQRAGRFELADGGTLLLDEIGEISPAFQAKLLRVLQEGELERVGGTKTLKVDVRLICATNKDLETAV
AEGEFRADLYYRINVVPLFLPPLRERNGDIPRLAKVFLDRFNRENNRNLEFTPAALEILSRCKFPGNVRELENCVRRTAT
LARSATIVPSDFSCQNDQCFSSMLGKTADRPLGSHSLNGLAMSKRLPVESPASLGYPAGPGGLTVAPHLSDRELLISAME
KAGWVQAKAARILGLTPRQVGYALRRHHIQVKKI
>Mature_594_residues
MQAQALDNVSKAAFSRREPRAVRTTLKVFPRKNWLDECMTELSPKTMHKRDLGCSGLYRISKVLITSASLEIKLANVINT
LPALLPMRRGAIVVVGAEGEPETTATFGVEPPSSGARHIAAKAAIDRIVAKGAPLVVPDTCKSELFQDELQSIVSGTGQV
TFIGVPMKADQETLGTLWIDRAKDGAATRTQFEEEVRFLSMVANLAARAVRLNGHESRDSRPIGEEGDRKISGGDKELPE
PTRQRPTRIDWIVGESPALRQVVESVKVVAQTNSAVLLRGESGTGKEFFAKAIHELSPRRKKPFVKLNCAALSAGVLESE
LFGHEKGAFTGAISQRAGRFELADGGTLLLDEIGEISPAFQAKLLRVLQEGELERVGGTKTLKVDVRLICATNKDLETAV
AEGEFRADLYYRINVVPLFLPPLRERNGDIPRLAKVFLDRFNRENNRNLEFTPAALEILSRCKFPGNVRELENCVRRTAT
LARSATIVPSDFSCQNDQCFSSMLGKTADRPLGSHSLNGLAMSKRLPVESPASLGYPAGPGGLTVAPHLSDRELLISAME
KAGWVQAKAARILGLTPRQVGYALRRHHIQVKKI

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI87082117, Length=237, Percent_Identity=55.6962025316456, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1789087, Length=233, Percent_Identity=57.0815450643777, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1790437, Length=338, Percent_Identity=44.6745562130178, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788550, Length=291, Percent_Identity=47.0790378006873, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=44.5820433436532, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1790299, Length=337, Percent_Identity=42.1364985163205, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI87081872, Length=346, Percent_Identity=41.6184971098266, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788905, Length=238, Percent_Identity=49.1596638655462, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1789233, Length=245, Percent_Identity=44.8979591836735, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=258, Percent_Identity=43.4108527131783, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=45.3061224489796, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=39.4422310756972, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI87081858, Length=238, Percent_Identity=31.5126050420168, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFA_RHISN (Q53206)

Other databases:

- EMBL:   Z68203
- EMBL:   U00090
- RefSeq:   NP_444099.1
- ProteinModelPortal:   Q53206
- SMR:   Q53206
- GeneID:   962486
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01260
- HOGENOM:   HBG740884
- GO:   GO:0005622
- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00065
- TIGRFAMs:   TIGR01817

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 64886; Mature: 64886

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQALDNVSKAAFSRREPRAVRTTLKVFPRKNWLDECMTELSPKTMHKRDLGCSGLYRI
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHCCCCHHHHHH
SKVLITSASLEIKLANVINTLPALLPMRRGAIVVVGAEGEPETTATFGVEPPSSGARHIA
HHHHHHHCCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHH
AKAAIDRIVAKGAPLVVPDTCKSELFQDELQSIVSGTGQVTFIGVPMKADQETLGTLWID
HHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHEEE
RAKDGAATRTQFEEEVRFLSMVANLAARAVRLNGHESRDSRPIGEEGDRKISGGDKELPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
PTRQRPTRIDWIVGESPALRQVVESVKVVAQTNSAVLLRGESGTGKEFFAKAIHELSPRR
HHHCCCCEEEEEECCCHHHHHHHHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHCCCHH
KKPFVKLNCAALSAGVLESELFGHEKGAFTGAISQRAGRFELADGGTLLLDEIGEISPAF
CCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEECCCCEEHHHHHCCCCHHH
QAKLLRVLQEGELERVGGTKTLKVDVRLICATNKDLETAVAEGEFRADLYYRINVVPLFL
HHHHHHHHHCCCHHHCCCCEEEEEEEEEEEECCCCHHHHHHCCCEEEEEEEEEEEEEEEC
PPLRERNGDIPRLAKVFLDRFNRENNRNLEFTPAALEILSRCKFPGNVRELENCVRRTAT
CCHHHCCCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LARSATIVPSDFSCQNDQCFSSMLGKTADRPLGSHSLNGLAMSKRLPVESPASLGYPAGP
HHHCCEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCC
GGLTVAPHLSDRELLISAMEKAGWVQAKAARILGLTPRQVGYALRRHHIQVKKI
CCCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCEEEECC
>Mature Secondary Structure
MQAQALDNVSKAAFSRREPRAVRTTLKVFPRKNWLDECMTELSPKTMHKRDLGCSGLYRI
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHCCCCHHHHHH
SKVLITSASLEIKLANVINTLPALLPMRRGAIVVVGAEGEPETTATFGVEPPSSGARHIA
HHHHHHHCCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHH
AKAAIDRIVAKGAPLVVPDTCKSELFQDELQSIVSGTGQVTFIGVPMKADQETLGTLWID
HHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHEEE
RAKDGAATRTQFEEEVRFLSMVANLAARAVRLNGHESRDSRPIGEEGDRKISGGDKELPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
PTRQRPTRIDWIVGESPALRQVVESVKVVAQTNSAVLLRGESGTGKEFFAKAIHELSPRR
HHHCCCCEEEEEECCCHHHHHHHHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHCCCHH
KKPFVKLNCAALSAGVLESELFGHEKGAFTGAISQRAGRFELADGGTLLLDEIGEISPAF
CCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEECCCCEEHHHHHCCCCHHH
QAKLLRVLQEGELERVGGTKTLKVDVRLICATNKDLETAVAEGEFRADLYYRINVVPLFL
HHHHHHHHHCCCHHHCCCCEEEEEEEEEEEECCCCHHHHHHCCCEEEEEEEEEEEEEEEC
PPLRERNGDIPRLAKVFLDRFNRENNRNLEFTPAALEILSRCKFPGNVRELENCVRRTAT
CCHHHCCCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LARSATIVPSDFSCQNDQCFSSMLGKTADRPLGSHSLNGLAMSKRLPVESPASLGYPAGP
HHHCCEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCC
GGLTVAPHLSDRELLISAMEKAGWVQAKAARILGLTPRQVGYALRRHHIQVKKI
CCCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424