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The map label for this gene is nifB

Identifier: 16519978

GI number: 16519978

Start: 156863

End: 158344

Strand: Direct

Name: nifB

Synonym: NGR_a01270

Alternate gene names: 16519978

Gene position: 156863-158344 (Clockwise)

Preceding gene: 16519979

Following gene: 16519977

Centisome position: 29.26

GC content: 58.43

Gene sequence:

>1482_bases
ATGAGTGCACCGATAATTTCGCTTGAAAGCCTGACGAACACGACATCCTCTGGGCAGTTGCTGACAACCGCAAAATCCGG
TGGTTGCGCGTCCTCGTCCTGCGGCTCGTCCTCAAGGCCAACCGACATGGATTCTGCCACCTGGGAGAAGATCAAGGATC
ACCCTTGCTTTTCAGAGGAAGCGCACCACTATTTCGCCCGTATGCACGTCGCAGTCGCACCAGCCTGCAACATCCAATGC
AATTACTGCAATCGCAAATATGACTGCGCCAACGAAAGTCGGCCTGGCGTCGTATCCGAAAAGCTGACACCCGACCAGGC
GCTGCGCAAGGTGGTTGCGGTCGCCAACGAAGTGCCGCAGCTTTCAGTTCTCGGCATCGCGGGCCCGGGCGATGCTTGCT
ACGACTGGAACAAGACAAGGGCGACTTTCGAACGCGTCGCCAGCGAGATTCCGGACATCAAGCTGTGCATTTCCACCAAT
GGACTTGCTCTACCGGAGCATGTCGACAAGTTGGCGGAAATGAACGTCAGCCACGTGACAATCACCATCAACATGGTCGA
CCCCGCCGTCGGCGAGAAGATCTACCCGTGGATCTTTTATGGGCATCGCCGCTACACCGGCGTGGATGCTGCCAAAATCC
TGCATGAGCAGCAGATGTTGGGACTTGAGATGCTGACCGCACGCGGCATCCTCACCAAGATCAATTCGGTTATGATCCCC
GGCGTCAATGACCTGCACCTGATCGAGGTCAACAAATGGGTCAAGGAGCGCGGCGCGTTTCTGCACAATGTCATGCCGCT
GATTTCAGACCCGGCACACGGAACCTCCTTTGGCCTGACCGGACAGCGCGGCCCAAATGCGCTCGAACTGAAGGCGCTTC
ACGACCGTCTTGAAGGCGGTGCCAAGCTGATGCGTCACTGTCGACAGTGCCGTGCCGATGCAGTCGGCCTTCTCGGCACT
GACCGTGGCCAGGAGTTCACGCTCGACCACGTTCCGCTCGAACCCCATTACGACGGTGCCAAGCGTCAGGCCTACCGGGA
GGTGGTCGCACGCATACGCGATGACCATTTAGAGGCTAAGGAGAAAGCGATCGCAACGGTCGCATCTGCGAACATCCGTG
GATCGCTTCAGGTGGCTGTCGCAACGAAAGGCGGCGGACGCATCAATGAACACTTCGGCCATGCGAAGGAGTTCCAGGTT
TACGAAGCCTCGCAAACAGGGATTAAATTCGTGGGGCACCGCAAGGTCGAGCCGTATTGCCATGGCGGCTGGGGCGAGGA
CGCCGCCCTCGCTGGCATCATCGCTGCACTCGACGGCATAGACATCGTGCTCTGCGCAAGGATAGGAGATTGTCCGAAGG
AGAGGCTCATGGAAGCTGGGATCCGGGCAACGGACACCTATGGCTATGACTACATCGAGGCCGCGATCAGCGCTCTTCAC
GCGACAGAGTTTGGTACCGCCCCATCGCAGGCAACGGCTTGA

Upstream 100 bases:

>100_bases
TCGAACACGAAGCGCCAAACTATTGATATTTCGATATTATCCTGTTGGCACAGCGCTTGCTTTTTTGGACGACGACTTTT
TACTCCAGTGGAGCCGTTTG

Downstream 100 bases:

>100_bases
GCTTGGCCCATCGATCGAGACCTAACCAGGAGCTGAATAATATGGCCTTCAAAATCATCGCATCCCAGTGCACCCAGTGC
GGCGCCTGCGAGTTCGAATG

Product: nitrogen fixation protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSAPIISLESLTNTTSSGQLLTTAKSGGCASSSCGSSSRPTDMDSATWEKIKDHPCFSEEAHHYFARMHVAVAPACNIQC
NYCNRKYDCANESRPGVVSEKLTPDQALRKVVAVANEVPQLSVLGIAGPGDACYDWNKTRATFERVASEIPDIKLCISTN
GLALPEHVDKLAEMNVSHVTITINMVDPAVGEKIYPWIFYGHRRYTGVDAAKILHEQQMLGLEMLTARGILTKINSVMIP
GVNDLHLIEVNKWVKERGAFLHNVMPLISDPAHGTSFGLTGQRGPNALELKALHDRLEGGAKLMRHCRQCRADAVGLLGT
DRGQEFTLDHVPLEPHYDGAKRQAYREVVARIRDDHLEAKEKAIATVASANIRGSLQVAVATKGGGRINEHFGHAKEFQV
YEASQTGIKFVGHRKVEPYCHGGWGEDAALAGIIAALDGIDIVLCARIGDCPKERLMEAGIRATDTYGYDYIEAAISALH
ATEFGTAPSQATA

Sequences:

>Translated_493_residues
MSAPIISLESLTNTTSSGQLLTTAKSGGCASSSCGSSSRPTDMDSATWEKIKDHPCFSEEAHHYFARMHVAVAPACNIQC
NYCNRKYDCANESRPGVVSEKLTPDQALRKVVAVANEVPQLSVLGIAGPGDACYDWNKTRATFERVASEIPDIKLCISTN
GLALPEHVDKLAEMNVSHVTITINMVDPAVGEKIYPWIFYGHRRYTGVDAAKILHEQQMLGLEMLTARGILTKINSVMIP
GVNDLHLIEVNKWVKERGAFLHNVMPLISDPAHGTSFGLTGQRGPNALELKALHDRLEGGAKLMRHCRQCRADAVGLLGT
DRGQEFTLDHVPLEPHYDGAKRQAYREVVARIRDDHLEAKEKAIATVASANIRGSLQVAVATKGGGRINEHFGHAKEFQV
YEASQTGIKFVGHRKVEPYCHGGWGEDAALAGIIAALDGIDIVLCARIGDCPKERLMEAGIRATDTYGYDYIEAAISALH
ATEFGTAPSQATA
>Mature_492_residues
SAPIISLESLTNTTSSGQLLTTAKSGGCASSSCGSSSRPTDMDSATWEKIKDHPCFSEEAHHYFARMHVAVAPACNIQCN
YCNRKYDCANESRPGVVSEKLTPDQALRKVVAVANEVPQLSVLGIAGPGDACYDWNKTRATFERVASEIPDIKLCISTNG
LALPEHVDKLAEMNVSHVTITINMVDPAVGEKIYPWIFYGHRRYTGVDAAKILHEQQMLGLEMLTARGILTKINSVMIPG
VNDLHLIEVNKWVKERGAFLHNVMPLISDPAHGTSFGLTGQRGPNALELKALHDRLEGGAKLMRHCRQCRADAVGLLGTD
RGQEFTLDHVPLEPHYDGAKRQAYREVVARIRDDHLEAKEKAIATVASANIRGSLQVAVATKGGGRINEHFGHAKEFQVY
EASQTGIKFVGHRKVEPYCHGGWGEDAALAGIIAALDGIDIVLCARIGDCPKERLMEAGIRATDTYGYDYIEAAISALHA
TEFGTAPSQATA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFB_RHISN (Q53205)

Other databases:

- EMBL:   Z68203
- EMBL:   U00090
- RefSeq:   NP_444098.1
- ProteinModelPortal:   Q53205
- SMR:   Q53205
- GeneID:   962467
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01270
- HOGENOM:   HBG424428
- ProtClustDB:   CLSK864402
- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.30.420.130
- SMART:   SM00729
- TIGRFAMs:   TIGR01290

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM; SSF53146 FeMo-co_biosynth

EC number: NA

Molecular weight: Translated: 53507; Mature: 53376

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPIISLESLTNTTSSGQLLTTAKSGGCASSSCGSSSRPTDMDSATWEKIKDHPCFSEE
CCCCEEEHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHH
AHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPDQALRKVVAVANEVPQ
HHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
LSVLGIAGPGDACYDWNKTRATFERVASEIPDIKLCISTNGLALPEHVDKLAEMNVSHVT
EEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCEEEE
ITINMVDPAVGEKIYPWIFYGHRRYTGVDAAKILHEQQMLGLEMLTARGILTKINSVMIP
EEEEEECCCCCCCCCCEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
GVNDLHLIEVNKWVKERGAFLHNVMPLISDPAHGTSFGLTGQRGPNALELKALHDRLEGG
CCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCH
AKLMRHCRQCRADAVGLLGTDRGQEFTLDHVPLEPHYDGAKRQAYREVVARIRDDHLEAK
HHHHHHHHHHHHHCEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EKAIATVASANIRGSLQVAVATKGGGRINEHFGHAKEFQVYEASQTGIKFVGHRKVEPYC
HHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCCCHHEEEEECCCCCEEEEECCCCCCCC
HGGWGEDAALAGIIAALDGIDIVLCARIGDCPKERLMEAGIRATDTYGYDYIEAAISALH
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCEECCCCHHHHHHHHHHHH
ATEFGTAPSQATA
HHHCCCCCCCCCC
>Mature Secondary Structure 
SAPIISLESLTNTTSSGQLLTTAKSGGCASSSCGSSSRPTDMDSATWEKIKDHPCFSEE
CCCEEEHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHH
AHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPDQALRKVVAVANEVPQ
HHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
LSVLGIAGPGDACYDWNKTRATFERVASEIPDIKLCISTNGLALPEHVDKLAEMNVSHVT
EEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCEEEE
ITINMVDPAVGEKIYPWIFYGHRRYTGVDAAKILHEQQMLGLEMLTARGILTKINSVMIP
EEEEEECCCCCCCCCCEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
GVNDLHLIEVNKWVKERGAFLHNVMPLISDPAHGTSFGLTGQRGPNALELKALHDRLEGG
CCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCH
AKLMRHCRQCRADAVGLLGTDRGQEFTLDHVPLEPHYDGAKRQAYREVVARIRDDHLEAK
HHHHHHHHHHHHHCEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EKAIATVASANIRGSLQVAVATKGGGRINEHFGHAKEFQVYEASQTGIKFVGHRKVEPYC
HHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCCCHHEEEEECCCCCEEEEECCCCCCCC
HGGWGEDAALAGIIAALDGIDIVLCARIGDCPKERLMEAGIRATDTYGYDYIEAAISALH
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCEECCCCHHHHHHHHHHHH
ATEFGTAPSQATA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424