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Identifier: 16519907

GI number: 16519907

Start: 259575

End: 260591

Strand: Direct

Name: Not Available

Synonym: NGR_a01980

Alternate gene names: 16519907

Gene position: 259575-260591 (Clockwise)

Preceding gene: 16519909

Following gene: 16519904

Centisome position: 48.41

GC content: 62.83

Gene sequence:

>1017_bases
GTGGATGAGCCCGCTTGTAATGGCACCTCGAACGAATGGCAGCGGCCGCAACTGATCGTCGCCGGCCCTCGCGCCGCACG
GCGACGGCAAATGTTGGCATCGCTTGACCTGAACACGCTGTTGGCTCTCGAGGCGCTGCTCGAGCACCGCAACGTGACGC
AGGCAGCCCGGCACCTGGGCCTGAGCCAACCGTCGGTAAGCCGGGCCTTGATCAGGCTGCGTGGCGTTTTCAATGATGAT
CTGTTGGTTCGCGGCTCAAGCGGCATGGTTCCGACGCCTCACGCCCAACGCCTGGGCCAGATGCTACCGCCGGTGTTGGA
TTCGATCCGCGGGATGGTGGACCCCGGCCTTGATCAGGGAGAATGGCGATTAACGGCAAGAATGGCAATGCCTGATCATC
AAGCGATCGTGCTGCTACCGCCTTTCCTGCCGCTGATGCGCGAGCGCGCGCCTAACCTGGACATAGTGACCGATTCGCTC
CTAGCGCTCCGACGCCTTGAGCAAGGCGAAATTGATTTAGCCGTTGGCCAGATCGGTGAGGCTCCACCGGGCTACTTTCG
GCGCAGGCTCTACAACGACCGCTTTGCTTGCCTGCTACGCAACGGCCACCCAGCCCTGGAGCAGGAATCGATAATCGACA
CCTTCTCAGCCCTGCGCCACGCCGCCATTGCCTCGGATACCAAAGACGGTTTCGGCCGGGTGCATGATGATCTGGTCAAG
TTGGATCTCCAGGATCCCGATCCGGTGCTGGTTTCCAATGTCCTGACGGCCGGATTGGCGATCGTGTCGACCGATCTGGT
GCTGGTCGTACCACGCCGCGTCGCGACCAGGAACGCCGCCTTGCTGCCGCTAGTGATTGTCGACCCGCCTGTGGAACTGC
CGCCCTACGAGGTCGCGCTGATCTGGCACGAGCGTTGCCATCGCGACCCGGACCATCGGTGGTTGCGCCAGGAGATCGCC
GCCGCGGCAACGGCAACAGAGCAAGGGCAGTCGACCGATGCTGCGTCCAGGCAATGA

Upstream 100 bases:

>100_bases
AAACCCCTGATGTGCTCAGCATGCTCGGGCTACTCCGCACCGCCAGTTCTCCGAGACGTTAGCCTGAGCACACTGCGTAC
GAAGACAGAAGGAGAGGCTC

Downstream 100 bases:

>100_bases
GTCCGAAATCTGGCCGCCTTCGGTCCATATCGGCGACGGTTGGTTACCCTCTCCTCATGCGCCGGCCGGCGAAGACGCCG
CTGCGCCAATACAACGAGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Symbiotic regulator homolog 1

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MDEPACNGTSNEWQRPQLIVAGPRAARRRQMLASLDLNTLLALEALLEHRNVTQAARHLGLSQPSVSRALIRLRGVFNDD
LLVRGSSGMVPTPHAQRLGQMLPPVLDSIRGMVDPGLDQGEWRLTARMAMPDHQAIVLLPPFLPLMRERAPNLDIVTDSL
LALRRLEQGEIDLAVGQIGEAPPGYFRRRLYNDRFACLLRNGHPALEQESIIDTFSALRHAAIASDTKDGFGRVHDDLVK
LDLQDPDPVLVSNVLTAGLAIVSTDLVLVVPRRVATRNAALLPLVIVDPPVELPPYEVALIWHERCHRDPDHRWLRQEIA
AAATATEQGQSTDAASRQ

Sequences:

>Translated_338_residues
MDEPACNGTSNEWQRPQLIVAGPRAARRRQMLASLDLNTLLALEALLEHRNVTQAARHLGLSQPSVSRALIRLRGVFNDD
LLVRGSSGMVPTPHAQRLGQMLPPVLDSIRGMVDPGLDQGEWRLTARMAMPDHQAIVLLPPFLPLMRERAPNLDIVTDSL
LALRRLEQGEIDLAVGQIGEAPPGYFRRRLYNDRFACLLRNGHPALEQESIIDTFSALRHAAIASDTKDGFGRVHDDLVK
LDLQDPDPVLVSNVLTAGLAIVSTDLVLVVPRRVATRNAALLPLVIVDPPVELPPYEVALIWHERCHRDPDHRWLRQEIA
AAATATEQGQSTDAASRQ
>Mature_338_residues
MDEPACNGTSNEWQRPQLIVAGPRAARRRQMLASLDLNTLLALEALLEHRNVTQAARHLGLSQPSVSRALIRLRGVFNDD
LLVRGSSGMVPTPHAQRLGQMLPPVLDSIRGMVDPGLDQGEWRLTARMAMPDHQAIVLLPPFLPLMRERAPNLDIVTDSL
LALRRLEQGEIDLAVGQIGEAPPGYFRRRLYNDRFACLLRNGHPALEQESIIDTFSALRHAAIASDTKDGFGRVHDDLVK
LDLQDPDPVLVSNVLTAGLAIVSTDLVLVVPRRVATRNAALLPLVIVDPPVELPPYEVALIWHERCHRDPDHRWLRQEIA
AAATATEQGQSTDAASRQ

Specific function: Transcriptional activator that regulates the expression of genes involved in symbiosis. Among other targets it acts on the nolWBTUV operon

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI87081684, Length=312, Percent_Identity=24.6794871794872, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI87081766, Length=292, Percent_Identity=24.3150684931507, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790147, Length=299, Percent_Identity=25.0836120401338, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYRM1_RHISN (P55619)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444027.1
- ProteinModelPortal:   P55619
- SMR:   P55619
- GeneID:   962183
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01980
- HOGENOM:   HBG682110
- ProtClustDB:   CLSK807611
- GO:   GO:0009877
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 37350; Mature: 37350

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEPACNGTSNEWQRPQLIVAGPRAARRRQMLASLDLNTLLALEALLEHRNVTQAARHLG
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCC
LSQPSVSRALIRLRGVFNDDLLVRGSSGMVPTPHAQRLGQMLPPVLDSIRGMVDPGLDQG
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EWRLTARMAMPDHQAIVLLPPFLPLMRERAPNLDIVTDSLLALRRLEQGEIDLAVGQIGE
CEEEEEEEECCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
APPGYFRRRLYNDRFACLLRNGHPALEQESIIDTFSALRHAAIASDTKDGFGRVHDDLVK
CCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEE
LDLQDPDPVLVSNVLTAGLAIVSTDLVLVVPRRVATRNAALLPLVIVDPPVELPPYEVAL
ECCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHCCCCEEEEEEECCCCCCCCCEEEE
IWHERCHRDPDHRWLRQEIAAAATATEQGQSTDAASRQ
EEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDEPACNGTSNEWQRPQLIVAGPRAARRRQMLASLDLNTLLALEALLEHRNVTQAARHLG
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCC
LSQPSVSRALIRLRGVFNDDLLVRGSSGMVPTPHAQRLGQMLPPVLDSIRGMVDPGLDQG
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EWRLTARMAMPDHQAIVLLPPFLPLMRERAPNLDIVTDSLLALRRLEQGEIDLAVGQIGE
CEEEEEEEECCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
APPGYFRRRLYNDRFACLLRNGHPALEQESIIDTFSALRHAAIASDTKDGFGRVHDDLVK
CCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEE
LDLQDPDPVLVSNVLTAGLAIVSTDLVLVVPRRVATRNAALLPLVIVDPPVELPPYEVAL
ECCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHCCCCEEEEEEECCCCCCCCCEEEE
IWHERCHRDPDHRWLRQEIAAAATATEQGQSTDAASRQ
EEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424