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The map label for this gene is potD [H]

Identifier: 16273254

GI number: 16273254

Start: 1420204

End: 1421343

Strand: Reverse

Name: potD [H]

Synonym: HI1344

Alternate gene names: 16273254

Gene position: 1421343-1420204 (Counterclockwise)

Preceding gene: 16273255

Following gene: 16273252

Centisome position: 77.66

GC content: 37.81

Gene sequence:

>1140_bases
ATGCCTGTCATTCGGCAGGTGGTTTTTTACGACTCTTTAACAGGAGAACAAACAAAAATGAAAAAATTTGCAGGTTTAAT
TACTGCCAGCTTCGTAGCTGCAACTTTAACCGCTTGCAACGATAAAGATGCTAAGCAAGAAACTGCAAAAGCTACGGCTG
CTGCTAATGACACTGTGTATCTCTACACTTGGACAGAATATGTACCAGATGGTCTTTTAGATGAGTTTACTAAAGAAACT
GGCATCAAAGTTATCGTTTCAAGCCTAGAATCAAACGAAACAATGTACGCTAAACTCAAAACTCAAGGCGAGTCTGGTGG
CTATGATGTTATCGCACCTTCTAACTACTTCGTTTCTAAAATGGCACGCGAAGGAATGTTAAAAGAACTTGATCACAGCA
AATTACCTGTTCTTAAAGAATTAGATCCTGATTGGCTCAATAAACCTTATGATAAAGGTAACAAATACTCTCTTCCGCAA
CTTTTAGGTGCGCCGGGTATCGCATTCAATACCAACACTTACAAAGGCGAACAATTCACTTCTTGGGCAGACTTATGGAA
ACCTGAATTTGCAAACAAAGTTCAATTATTAGACGATGCGCGTGAAGTATTCAACATCGCTTTATTGAAAATTGGTCAAG
ATCCAAATACTCAAGATCCAGCCATTATCAAACAAGCCTATGAAGAATTATTGAAATTACGTCCAAATGTACTTTCTTTC
AATTCCGATAACCCAGCTAACTCGTTCATCTCAGGCGAAGTGGAAGTGGGTCAATTATGGAATGGTTCGGTACGTATTGC
TAAAAAAGAAAAAGCACCTTTAAATATGGTATTCCCAAAAGAGGGTCCTGTACTTTGGGTTGATACTCTTGCAATTCCTG
CAACAGCTAAAAATTCTGAAGGCGCACACAAGCTGATTAACTACATGTTAGGGAAAAAAACAGCTGAAAAATTAACCTTA
GCTATCGGTTACCCAACCTCAAATATTGAAGCGAAAAAAGCATTACCAAAAGAAATCACTGAAGATCCAGCGATTTATCC
GTCAGCTGATATATTAAAAAATAGTCACTGGCAAGATGACGTTGGCGATGCAATTCAATTCTATGAACAATATTATCAAG
AATTAAAAGCAGCAAAATAA

Upstream 100 bases:

>100_bases
GCTAATTACACGAAAAAATAACCATTAATTAACAAAAGGTTTCACAAACCTTTGAATTTAAAATAGAATACGCCTTGACG
CACAATCAAGGCGTTTTTTT

Downstream 100 bases:

>100_bases
TTGGTCAATTAGCAAAGAATTAAAATATGATTCTGTGCAATATTTCCTCTAAATAAAAACGCCCTTTCGGGCGTTATTTT
TTAAGCTTTACCGTCAGCCA

Product: spermidine/putrescine ABC transporter periplasmic-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MPVIRQVVFYDSLTGEQTKMKKFAGLITASFVAATLTACNDKDAKQETAKATAAANDTVYLYTWTEYVPDGLLDEFTKET
GIKVIVSSLESNETMYAKLKTQGESGGYDVIAPSNYFVSKMAREGMLKELDHSKLPVLKELDPDWLNKPYDKGNKYSLPQ
LLGAPGIAFNTNTYKGEQFTSWADLWKPEFANKVQLLDDAREVFNIALLKIGQDPNTQDPAIIKQAYEELLKLRPNVLSF
NSDNPANSFISGEVEVGQLWNGSVRIAKKEKAPLNMVFPKEGPVLWVDTLAIPATAKNSEGAHKLINYMLGKKTAEKLTL
AIGYPTSNIEAKKALPKEITEDPAIYPSADILKNSHWQDDVGDAIQFYEQYYQELKAAK

Sequences:

>Translated_379_residues
MPVIRQVVFYDSLTGEQTKMKKFAGLITASFVAATLTACNDKDAKQETAKATAAANDTVYLYTWTEYVPDGLLDEFTKET
GIKVIVSSLESNETMYAKLKTQGESGGYDVIAPSNYFVSKMAREGMLKELDHSKLPVLKELDPDWLNKPYDKGNKYSLPQ
LLGAPGIAFNTNTYKGEQFTSWADLWKPEFANKVQLLDDAREVFNIALLKIGQDPNTQDPAIIKQAYEELLKLRPNVLSF
NSDNPANSFISGEVEVGQLWNGSVRIAKKEKAPLNMVFPKEGPVLWVDTLAIPATAKNSEGAHKLINYMLGKKTAEKLTL
AIGYPTSNIEAKKALPKEITEDPAIYPSADILKNSHWQDDVGDAIQFYEQYYQELKAAK
>Mature_378_residues
PVIRQVVFYDSLTGEQTKMKKFAGLITASFVAATLTACNDKDAKQETAKATAAANDTVYLYTWTEYVPDGLLDEFTKETG
IKVIVSSLESNETMYAKLKTQGESGGYDVIAPSNYFVSKMAREGMLKELDHSKLPVLKELDPDWLNKPYDKGNKYSLPQL
LGAPGIAFNTNTYKGEQFTSWADLWKPEFANKVQLLDDAREVFNIALLKIGQDPNTQDPAIIKQAYEELLKLRPNVLSFN
SDNPANSFISGEVEVGQLWNGSVRIAKKEKAPLNMVFPKEGPVLWVDTLAIPATAKNSEGAHKLINYMLGKKTAEKLTLA
IGYPTSNIEAKKALPKEITEDPAIYPSADILKNSHWQDDVGDAIQFYEQYYQELKAAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=329, Percent_Identity=54.4072948328268, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1787078, Length=319, Percent_Identity=35.423197492163, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42183; Mature: 42052

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIRQVVFYDSLTGEQTKMKKFAGLITASFVAATLTACNDKDAKQETAKATAAANDTVY
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEE
LYTWTEYVPDGLLDEFTKETGIKVIVSSLESNETMYAKLKTQGESGGYDVIAPSNYFVSK
EEEEHHHCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCHHHHHH
MAREGMLKELDHSKLPVLKELDPDWLNKPYDKGNKYSLPQLLGAPGIAFNTNTYKGEQFT
HHHHHHHHHCCCCCCCHHHCCCHHHCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCC
SWADLWKPEFANKVQLLDDAREVFNIALLKIGQDPNTQDPAIIKQAYEELLKLRPNVLSF
CHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEE
NSDNPANSFISGEVEVGQLWNGSVRIAKKEKAPLNMVFPKEGPVLWVDTLAIPATAKNSE
CCCCCCCCEECCCEEECEECCCCEEEEECCCCCCEEECCCCCCEEEEEEEECCCCCCCCC
GAHKLINYMLGKKTAEKLTLAIGYPTSNIEAKKALPKEITEDPAIYPSADILKNSHWQDD
HHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHCCCHHHCCCCCCCCCHHHHCCCCCCCH
VGDAIQFYEQYYQELKAAK
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVIRQVVFYDSLTGEQTKMKKFAGLITASFVAATLTACNDKDAKQETAKATAAANDTVY
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEE
LYTWTEYVPDGLLDEFTKETGIKVIVSSLESNETMYAKLKTQGESGGYDVIAPSNYFVSK
EEEEHHHCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCHHHHHH
MAREGMLKELDHSKLPVLKELDPDWLNKPYDKGNKYSLPQLLGAPGIAFNTNTYKGEQFT
HHHHHHHHHCCCCCCCHHHCCCHHHCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCC
SWADLWKPEFANKVQLLDDAREVFNIALLKIGQDPNTQDPAIIKQAYEELLKLRPNVLSF
CHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEE
NSDNPANSFISGEVEVGQLWNGSVRIAKKEKAPLNMVFPKEGPVLWVDTLAIPATAKNSE
CCCCCCCCEECCCEEECEECCCCEEEEECCCCCCEEECCCCCCEEEEEEEECCCCCCCCC
GAHKLINYMLGKKTAEKLTLAIGYPTSNIEAKKALPKEITEDPAIYPSADILKNSHWQDD
HHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHCCCHHHCCCCCCCCCHHHHCCCCCCCH
VGDAIQFYEQYYQELKAAK
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]