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The map label for this gene is metG [H]

Identifier: 16273191

GI number: 16273191

Start: 1351859

End: 1353907

Strand: Reverse

Name: metG [H]

Synonym: HI1276

Alternate gene names: 16273191

Gene position: 1353907-1351859 (Counterclockwise)

Preceding gene: 16273193

Following gene: 16273190

Centisome position: 73.98

GC content: 40.12

Gene sequence:

>2049_bases
ATGACAACTCAACCCCGTAAAATTTTAGTCACTTGCGCCTTGCCTTATGCCAATGGGGCAATTCATTTAGGCCATATGTT
AGAACATATCCAAGCGGATATTTGGGTGCGTTTTCAACGTATGCGTGGCAATAAAATCCATTTTGTCTGTGCGGACGATG
CGCACGGCACGCCGATTATGTTGAATGCTGATAAACTTGGCATTACACCTGAAGAATTAATTGCCAAAGCAAAAGCCGAT
CACATTCGTGATTTTGCAGGTTTTAATATTAGTTTTGATAACTATCATTCCACTCACAGCGAAGAAAACAAACAGCTCAC
CGCAGAAATTTACAATAAACTCAAAGCCAATGGTTTTATTAAGAGTAAAGTTATTTCTCAGTTATTCGACCCTGAAAAAA
ATATGTTCTTGCCTGATCGTTTTGTGAAAGGCACTTGCCCGAAATGCAAAGCAGAAGATCAATATGGCGATAACTGCGAA
GTGTGTGCTTCTACTTATAGCCCAATGGATTTAATTAATCCTCGTTCAGCTGTTTCTGGCACAACACCTATCGTAAAAGA
ATCTGAACACTTTTTCTTTGACTTGCCTGCATTTGAAGGGATGTTAAAAGAATGGACTCGTTCTGGCTCACTTCAATCTG
AAATTGCCAACAAAATGCAAGAATGGTTTGAGAGCGATTTACAACAATGGGATATTTCACGTGATGCACCTTATTTCGGT
TTTGAAATTCCCGGCGCAAAAGATAAATTCTTCTATGTTTGGTTAGATGCGCCAATCGGCTACATGGCTTCATTCAAAAA
TCTATGCAATCGTGAAGGCATTGATTTCAATGAATTTTGGGCTGAAGGCAGTGATGCAGAGCTTTATCATTTCATCGGTA
AAGACATCGTTTATTTCCACAGCCTATTCTGGCCTGCAATGCTTGAAGGCAGTGGCTACCGTAAACCGACTAATGTGTTC
GCTCATGGCTATGTCACTGTGGATGGGGCGAAGATGTCAAAATCTCGCGGCACATTCATTCAAGCCAGCACTTATTTGAA
TCATATCGATCCTGAATGTTTGCGTTATTACTATGCAGCAAAATTAAATGATCGCATTGAAGATTTAGACTTTAACCTTG
AAGATTTCGTACAACGTGTCAATACGGATATTGTAAACAAATTAGTGAATTTAGCTTCACGCAATGCGGGCTTTATTGCG
AAACGCTTTGAAGGCAAACTTGCGGATAAACTGGAAGACAAATCATTATTTGCAGAATTTACCGCCCAAGCCGAGCAAAT
CGCTGCTTATTACGAAAGCCGTGAATACAATAAAACCATTCGTGAGATTATGGCATTAACAGATAAAGCCAATAAATACA
TTGATGAAAAAGCCCCTTGGGTGATTGCAAAAGAAGAAGGCAAAGAGGCAGAATTGCAAGCCGTATGTTCAATGGGTATT
GAGCTTTTCCGCGTATTGATGTCTTACTTAAAACCTGTTCTTCCAAAACTAGCAGAACGTGCAGAAACCTTCTTACAAGC
TGAATTACGTTGGGATAATATTCATCAACCATTACTTGGTCATACCCTTGCACCGTTTAAAGCCTTATTCTCTCGTTTAG
AGAAAAAACAAATTGATGCCGTTGTGGAAGAAACTAAAGCCTTATTTGCAGCAGCCAATAAAGCCGCAGAAAAAACTGAA
GCAAAACCAACCGCACTTTCTGCCGTTGAACCTATTGCTGAAACCATCACGATTGATGATTTCGCTAAACTTGATATGCG
TGTAGCAAAAGTATTGAAATGCGAAGCGGTGCCAGAATCCAATAAACTTTTACGTTTTGAATTAGATCTTGGTGATCATA
CTCGCCAAGTCTTCTCTGGCATTAAAGCGGCTTACAATAAACCTGAAGAATTAGAAGGTCGTTTTGTGATTATGGTGGCA
AACCTTGCACCACGTAAAATGAAATTCGGCGTGTCTGAGGGAATGATTCTATCCGCAGGAACAGGCGGTAGTGACTTATT
CTTACTTTCTGCTGATAGCGGTGTCACTGCGGGCATGCAAGTAAAATAA

Upstream 100 bases:

>100_bases
AAAGTGCGGTATGTTAAGTTAAAGTTTTTCAAACTAGAAAAAATCCCCTATTTAAAGTAAGATAAGCACAATTTTTGTAT
GCTAAAAAAGAGAATAATAA

Downstream 100 bases:

>100_bases
TCAATTCAATCTAAATGAGGGCAACATTACGTTGCCTTTTTATTTTCTTGTTGAAAATAGATGACTTATAACCTACCATA
ACACTCAACTATTTATCTAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MTTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIMLNADKLGITPEELIAKAKAD
HIRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
VCASTYSPMDLINPRSAVSGTTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESDLQQWDISRDAPYFG
FEIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVF
AHGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAAKLNDRIEDLDFNLEDFVQRVNTDIVNKLVNLASRNAGFIA
KRFEGKLADKLEDKSLFAEFTAQAEQIAAYYESREYNKTIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGI
ELFRVLMSYLKPVLPKLAERAETFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDAVVEETKALFAAANKAAEKTE
AKPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPESNKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVA
NLAPRKMKFGVSEGMILSAGTGGSDLFLLSADSGVTAGMQVK

Sequences:

>Translated_682_residues
MTTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIMLNADKLGITPEELIAKAKAD
HIRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
VCASTYSPMDLINPRSAVSGTTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESDLQQWDISRDAPYFG
FEIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVF
AHGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAAKLNDRIEDLDFNLEDFVQRVNTDIVNKLVNLASRNAGFIA
KRFEGKLADKLEDKSLFAEFTAQAEQIAAYYESREYNKTIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGI
ELFRVLMSYLKPVLPKLAERAETFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDAVVEETKALFAAANKAAEKTE
AKPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPESNKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVA
NLAPRKMKFGVSEGMILSAGTGGSDLFLLSADSGVTAGMQVK
>Mature_681_residues
TTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIMLNADKLGITPEELIAKAKADH
IRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCEV
CASTYSPMDLINPRSAVSGTTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESDLQQWDISRDAPYFGF
EIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFA
HGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAAKLNDRIEDLDFNLEDFVQRVNTDIVNKLVNLASRNAGFIAK
RFEGKLADKLEDKSLFAEFTAQAEQIAAYYESREYNKTIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGIE
LFRVLMSYLKPVLPKLAERAETFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDAVVEETKALFAAANKAAEKTEA
KPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPESNKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVAN
LAPRKMKFGVSEGMILSAGTGGSDLFLLSADSGVTAGMQVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=635, Percent_Identity=28.503937007874, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI24308436, Length=515, Percent_Identity=25.631067961165, Blast_Score=171, Evalue=3e-42,
Organism=Escherichia coli, GI1788432, Length=684, Percent_Identity=68.2748538011696, Blast_Score=1015, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=583, Percent_Identity=28.9879931389365, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI212645096, Length=512, Percent_Identity=25.1953125, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=408, Percent_Identity=25.9803921568627, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321704, Length=554, Percent_Identity=29.2418772563177, Blast_Score=253, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=22.2018348623853, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655311, Length=555, Percent_Identity=29.009009009009, Blast_Score=257, Evalue=2e-68,
Organism=Drosophila melanogaster, GI21358483, Length=512, Percent_Identity=25.1953125, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 77024; Mature: 76892

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIM
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LNADKLGITPEELIAKAKADHIRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFI
EECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCH
KSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYSPMDLINPRSAVSG
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCHHCCC
TTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESDLQQWDISRDAPYFG
CCCCEECCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FEIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFH
EECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
SLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAA
HHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHH
KLNDRIEDLDFNLEDFVQRVNTDIVNKLVNLASRNAGFIAKRFEGKLADKLEDKSLFAEF
HHCCHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHH
TAQAEQIAAYYESREYNKTIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
ELFRVLMSYLKPVLPKLAERAETFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHH
VVEETKALFAAANKAAEKTEAKPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPES
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
NKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVANLAPRKMKFGVSEGMILSAG
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCHHCCCCCCCEEEEEC
TGGSDLFLLSADSGVTAGMQVK
CCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TTQPRKILVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGNKIHFVCADDAHGTPIM
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LNADKLGITPEELIAKAKADHIRDFAGFNISFDNYHSTHSEENKQLTAEIYNKLKANGFI
EECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCH
KSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCEVCASTYSPMDLINPRSAVSG
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCCHHCCC
TTPIVKESEHFFFDLPAFEGMLKEWTRSGSLQSEIANKMQEWFESDLQQWDISRDAPYFG
CCCCEECCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FEIPGAKDKFFYVWLDAPIGYMASFKNLCNREGIDFNEFWAEGSDAELYHFIGKDIVYFH
EECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
SLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSKSRGTFIQASTYLNHIDPECLRYYYAA
HHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHH
KLNDRIEDLDFNLEDFVQRVNTDIVNKLVNLASRNAGFIAKRFEGKLADKLEDKSLFAEF
HHCCHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHH
TAQAEQIAAYYESREYNKTIREIMALTDKANKYIDEKAPWVIAKEEGKEAELQAVCSMGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
ELFRVLMSYLKPVLPKLAERAETFLQAELRWDNIHQPLLGHTLAPFKALFSRLEKKQIDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHH
VVEETKALFAAANKAAEKTEAKPTALSAVEPIAETITIDDFAKLDMRVAKVLKCEAVPES
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
NKLLRFELDLGDHTRQVFSGIKAAYNKPEELEGRFVIMVANLAPRKMKFGVSEGMILSAG
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCHHCCCCCCCEEEEEC
TGGSDLFLLSADSGVTAGMQVK
CCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA